Rh2DG525200

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012811
Physical Location & Seq
Reverse (-)
75088817 .. 75091222
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG525200.1

Sequence Viewer

Length: 1059 bp
ATGACGAGTCTCAAAAGCATATCCCTAACCTTTCTTGTTGCTATTTTTGCAGTTTTATGGGCATGTAAACATCTCATAGGTTTTAATCCAAACTCAAAATACCTCAAATACTTTGAAGATCTTGGTCTCACCACCTCTTCAATATCACCTATTTTGTCTCTTCTCTTCCCATACTCCATCAACTTTAATGCTAGGCATCACAAAAAGAAAGATAATAAGGCCAAAAAAGCGTCAATTTGTGATGACTTCCCTCCTGGAATACCTCCTCCAGATACCAACACAACCTCCTATTTTTGCGTGGATCGAAATGGGTGCTGCAATTTTACTTTAGTTCAAGCAGCTGTTGATGCAGTTCCAGCTCTTAGCCAGAAAAGAACAGTATTATGGATCAATACTGGCATATACTATGAAAAAGTCATTGTTCCAATCACCAAACCAAACATAACATTCCAAGGACAAGGGTATTTATCGACTGCAATATCATGGAATGACACGGCGAATTCATCACATGGCACATTCTACAGTGGCTCTGTTCAAGTTTTTGGTGCCAACTTCATTGCTAAGAACATAAGCTTCATGAATGTGGCTCCAATTCCAAAACCTGGTGATTTTGGAGCGCAAGCTGTAGCAATTAGGGTATCGGGGGACCAATCTGCATTTTGGGGTTGTGGATTCTTTGGAGCTCAAGACACCCTCCATGATGATAAGGGCCGCCATTACTTTAAGGAATGCTATATTCAGGGGTCCATTGATTTCATCTTTGGCAATGCAAGGTCATTCTATGAGAATTGTCAGTTGGTTTCGATGGCAAACCCAGTAGCCCCAGGATCAAAGAGCATTAGCGGAGCCCTTACAGCGCATGGCAGGGTTTCAAATGAGAACACTGGATATGCATTTGTGAACTGCTTTGTTGGAGGGTCTGGAAGGGTTTTGTTAGGTCGAGCATGGAGGCCTTTCTCACGTGTGGTGTTTATCAACACAACAATGACAGACATCATAGCTCCAGAAGGCTGGAGTGATTTCAATGACCCGACAAGAGACCAGTACGTAAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

352

Amino Acids

38.66

Weight (kDa)

8.77

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 100 - 343 7.6e-58 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 602
AciI CCGC 2 cut(s) 712, 843
AclWI GGATC 3 cut(s) 309, 395, 835
AcsI RAATTY 1 cut(s) 499
AcvI CACGTG 1 cut(s) 962
AfaI GTAC 1 cut(s) 1046
AfiI CCNNNNNNNGG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 961
AgsI TTSAA 6 cut(s) 116, 141, 335, 536, 873, 1024
AjnI CCWGG 3 cut(s) 253, 601, 823
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AluBI AGCT 6 cut(s) 341, 359, 573, 623, 683, 1001
AluI AGCT 6 cut(s) 341, 359, 573, 623, 683, 1001
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 4 cut(s) 14, 131, 162, 1032
AlwI GGATC 3 cut(s) 309, 395, 835
AoxI GGCC 3 cut(s) 219, 709, 950
ApeKI GCWGC 2 cut(s) 315, 338
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 2 cut(s) 619, 859
AspS9I GGNCC 3 cut(s) 646, 709, 744
AsuHPI GGTGA 4 cut(s) 121, 138, 421, 617
AvaII GGWCC 2 cut(s) 646, 744
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 2 cut(s) 685, 850
BbrPI CACGTG 1 cut(s) 962
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 2 cut(s) 302, 350
BccI CCATC 2 cut(s) 185, 799
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 3 cut(s) 255, 603, 825
BcoDI GTCTC 4 cut(s) 14, 131, 162, 1032
BfaI CTAG 1 cut(s) 192
BfmI CTRYAG 2 cut(s) 520, 624
BglII AGATCT 1 cut(s) 118
BisI GCNGC 3 cut(s) 316, 339, 712
BlsI GCNGC 3 cut(s) 317, 340, 713
Bme1390I CCNGG 3 cut(s) 255, 603, 825
Bme18I GGWCC 2 cut(s) 646, 744
BmgT120I GGNCC 3 cut(s) 646, 709, 744
BmiI GGNNCC 5 cut(s) 547, 588, 647, 745, 847
BmrFI CCNGG 3 cut(s) 255, 603, 825
BmrI ACTGGG 1 cut(s) 809
BmsI GCATC 2 cut(s) 205, 337
BmuI ACTGGG 1 cut(s) 809
BpmI CTGGAG 3 cut(s) 252, 987, 1033
BpuEI CTTGAG 1 cut(s) 669
BsaAI YACGTR 2 cut(s) 962, 1048
BsaI GGTCTC 2 cut(s) 131, 1032
BsaJI CCNNGG 2 cut(s) 451, 823
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 400, 815, 889, 1042
Bse3DI GCAATG 2 cut(s) 555, 772
BseBI CCWGG 3 cut(s) 255, 603, 825
BseDI CCNNGG 2 cut(s) 451, 823
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMI GCAATG 2 cut(s) 555, 772
BseNI ACTGG 4 cut(s) 400, 815, 889, 1042
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 302, 350
BshFI GGCC 3 cut(s) 221, 711, 952
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 685
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 4 cut(s) 14, 131, 162, 1032
BsmFI GGGAC 1 cut(s) 659
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 3 cut(s) 221, 711, 952
Bso31I GGTCTC 2 cut(s) 131, 1032
Bsp1286I GDGCHC 2 cut(s) 685, 850
Bsp143I GATC 4 cut(s) 118, 301, 387, 827
BspACI CCGC 2 cut(s) 712, 843
BspANI GGCC 3 cut(s) 221, 711, 952
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 5 cut(s) 547, 588, 647, 745, 847
BspPI GGATC 3 cut(s) 309, 395, 835
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 2 cut(s) 131, 1032
BsrDI GCAATG 2 cut(s) 555, 772
BsrI ACTGG 4 cut(s) 400, 815, 889, 1042
BssECI CCNNGG 2 cut(s) 451, 823
BssMI GATC 4 cut(s) 118, 301, 387, 827
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 3 cut(s) 255, 603, 825
Bst4CI ACNGT 2 cut(s) 379, 524
Bst6I CTCTTC 3 cut(s) 142, 165, 170
BstBAI YACGTR 2 cut(s) 962, 1048
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 2 cut(s) 362, 561
BstHHI GCGC 2 cut(s) 619, 859
BstKTI GATC 4 cut(s) 121, 304, 390, 830
BstMAI GTCTC 4 cut(s) 14, 131, 162, 1032
BstMBI GATC 4 cut(s) 118, 301, 387, 827
BstMWI GCNNNNNNNGC 5 cut(s) 47, 227, 347, 356, 854
BstNI CCWGG 3 cut(s) 255, 603, 825
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 3 cut(s) 253, 601, 823
BstSFI CTRYAG 2 cut(s) 520, 624
BstSNI TACGTA 1 cut(s) 1048
BstV1I GCAGC 2 cut(s) 302, 350
BstX2I RGATCY 1 cut(s) 118
BstXI CCANNNNNNTGG 1 cut(s) 1011
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 3 cut(s) 221, 711, 952
BtsIMutI CAGTG 2 cut(s) 529, 882
Cac8I GCNNGC 1 cut(s) 621
CciI TCATGA 1 cut(s) 576
CfoI GCGC 2 cut(s) 619, 859
Cfr13I GGNCC 3 cut(s) 646, 709, 744
CseI GACGC 1 cut(s) 219
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 1 cut(s) 1045
CviAII CATG 7 cut(s) 63, 483, 509, 577, 698, 860, 945
CviQI GTAC 1 cut(s) 1045
DdeI CTNAG 2 cut(s) 362, 561
DpnI GATC 4 cut(s) 120, 303, 389, 829
DpnII GATC 4 cut(s) 118, 301, 387, 827
Eam1104I CTCTTC 3 cut(s) 142, 165, 170
EarI CTCTTC 3 cut(s) 142, 165, 170
Ecl136II GAGCTC 1 cut(s) 683
Eco105I TACGTA 1 cut(s) 1048
Eco130I CCWWGG 1 cut(s) 451
Eco147I AGGCCT 1 cut(s) 952
Eco24I GRGCYC 2 cut(s) 685, 850
Eco31I GGTCTC 2 cut(s) 131, 1032
Eco47I GGWCC 2 cut(s) 646, 744
Eco53kI GAGCTC 1 cut(s) 683
Eco72I CACGTG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 253, 601, 823
EcoT14I CCWWGG 1 cut(s) 451
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 2 cut(s) 685, 850
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 7 cut(s) 66, 486, 512, 580, 701, 863, 948
FaqI GGGAC 1 cut(s) 659
FatI CATG 7 cut(s) 62, 482, 508, 576, 697, 859, 944
Fnu4HI GCNGC 3 cut(s) 316, 339, 712
FriOI GRGCYC 2 cut(s) 685, 850
Fsp4HI GCNGC 3 cut(s) 316, 339, 712
FspBI CTAG 1 cut(s) 192
GlaI GCGC 2 cut(s) 618, 858
GluI GCNGC 3 cut(s) 316, 339, 712
GsuI CTGGAG 3 cut(s) 252, 987, 1033
HaeIII GGCC 3 cut(s) 221, 711, 952
HgaI GACGC 1 cut(s) 219
HhaI GCGC 2 cut(s) 619, 859
Hin1II CATG 7 cut(s) 66, 486, 512, 580, 701, 863, 948
Hin6I GCGC 2 cut(s) 617, 857
HinP1I GCGC 2 cut(s) 617, 857
HindIII AAGCTT 1 cut(s) 571
HinfI GANTC 2 cut(s) 7, 672
HphI GGTGA 4 cut(s) 121, 138, 421, 617
Hpy166II GTNNAC 3 cut(s) 68, 901, 1051
Hpy188III TCNNGA 5 cut(s) 269, 577, 686, 921, 1004
Hpy8I GTNNAC 3 cut(s) 68, 901, 1051
HpyAV CCTTC 2 cut(s) 918, 1001
HpyCH4III ACNGT 2 cut(s) 379, 524
HpyCH4IV ACGT 2 cut(s) 961, 1047
HpyCH4V TGCA 7 cut(s) 50, 318, 350, 476, 656, 770, 893
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 227, 347, 356, 854
HpyF3I CTNAG 2 cut(s) 362, 561
HpySE526I ACGT 2 cut(s) 961, 1047
Hsp92II CATG 7 cut(s) 66, 486, 512, 580, 701, 863, 948
HspAI GCGC 2 cut(s) 617, 857
Kzo9I GATC 4 cut(s) 118, 301, 387, 827
LmnI GCTCC 5 cut(s) 592, 614, 680, 845, 1006
Lsp1109I GCAGC 2 cut(s) 302, 350
LweI GCATC 2 cut(s) 205, 337
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 1 cut(s) 192
MaeII ACGT 2 cut(s) 961, 1047
MalI GATC 4 cut(s) 120, 303, 389, 829
MboI GATC 4 cut(s) 118, 301, 387, 827
MboII GAAGA 4 cut(s) 128, 129, 152, 157
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 2 cut(s) 685, 850
MluCI AATT 7 cut(s) 234, 319, 499, 591, 630, 787, 1054
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 892
MnlI CCTC 9 cut(s) 113, 145, 261, 273, 276, 295, 704, 908, 942
Mph1103I ATGCAT 1 cut(s) 895
MseI TTAA 3 cut(s) 84, 186, 723
MslI CAYNNNNRTG 2 cut(s) 581, 983
MspA1I CMGCKG 1 cut(s) 341
MspR9I CCNGG 3 cut(s) 255, 603, 825
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 3 cut(s) 255, 603, 825
MwoI GCNNNNNNNGC 5 cut(s) 47, 227, 347, 356, 854
NdeII GATC 4 cut(s) 118, 301, 387, 827
NlaIII CATG 7 cut(s) 66, 486, 512, 580, 701, 863, 948
NlaIV GGNNCC 5 cut(s) 547, 588, 647, 745, 847
NsiI ATGCAT 1 cut(s) 895
NspI RCATGY 1 cut(s) 66
PagI TCATGA 1 cut(s) 576
PceI AGGCCT 1 cut(s) 952
PctI GAATGC 1 cut(s) 734
PfeI GAWTC 1 cut(s) 672
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 3 cut(s) 317, 340, 713
PleI GAGTC 1 cut(s) 15
PmaCI CACGTG 1 cut(s) 962
PmlI CACGTG 1 cut(s) 962
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 2 cut(s) 962, 1048
Psp124BI GAGCTC 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 253, 601, 823
PspCI CACGTG 1 cut(s) 962
PspGI CCWGG 3 cut(s) 253, 601, 823
PspN4I GGNNCC 5 cut(s) 547, 588, 647, 745, 847
PspPI GGNCC 3 cut(s) 646, 709, 744
PsuI RGATCY 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 1046
RsaNI GTAC 1 cut(s) 1045
RseI CAYNNNNRTG 2 cut(s) 581, 983
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 3 cut(s) 84, 186, 723
SatI GCNGC 3 cut(s) 316, 339, 712
Sau3AI GATC 4 cut(s) 118, 301, 387, 827
Sau96I GGNCC 3 cut(s) 646, 709, 744
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 3 cut(s) 255, 603, 825
SduI GDGCHC 2 cut(s) 685, 850
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 205, 337
SfcI CTRYAG 2 cut(s) 520, 624
SinI GGWCC 2 cut(s) 646, 744
SmiMI CAYNNNNRTG 2 cut(s) 581, 983
SmlI CTYRAG 1 cut(s) 684
SmoI CTYRAG 1 cut(s) 684
SnaBI TACGTA 1 cut(s) 1048
Sse9I AATT 7 cut(s) 234, 319, 499, 591, 630, 787, 1054
SseBI AGGCCT 1 cut(s) 952
SsiI CCGC 2 cut(s) 712, 843
SspMI CTAG 1 cut(s) 192
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 952
StyD4I CCNGG 3 cut(s) 253, 601, 823
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 379, 524
TaiI ACGT 2 cut(s) 964, 1050
TaqI TCGA 4 cut(s) 304, 470, 803, 940
TasI AATT 7 cut(s) 234, 319, 499, 591, 630, 787, 1054
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 672
Tru1I TTAA 3 cut(s) 84, 186, 723
Tru9I TTAA 3 cut(s) 84, 186, 723
TscAI CASTG 2 cut(s) 529, 889
TseI GCWGC 2 cut(s) 315, 338
TspDTI ATGAA 6 cut(s) 423, 492, 544, 565, 593, 745
TspRI CASTG 2 cut(s) 529, 889
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 646, 744
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 66
XspI CTAG 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.