RchiOBHm_Chr2g0153521

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012811
Physical Location & Seq
Reverse (-)
70849039 .. 70851299
2261 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52259

Sequence Viewer

Length: 1266 bp
ATGTATGGTTCCAAATATCTAAGGTTCCGCTCAACCTCTTTTTCTTCTCCCTTTAATCAGAAAATGACGAGTCTCAAAAGCATATCCCTAACCTTTCTTGTTGCTATTTTTGCAGTTTTATGGGCATGTAAACATCTCATAGGTTTTAATCCAAACTCAAAATACCTCAAATACTTTGAAGATCTTGGTCTCACCACCTCTTCAATATCACCTATTTTGTCTCTTCTCTTCCCATACTCCATCAACTTTAATGCTAGGCATCACAAAAAGAAAGATAATAAGGCCAAAAAAGCGTCAATTTGTGATGACTTCCCTCCTGGAATACCTCCTCCAGATACCAACACAACCTCCTATTTTTGCGTGGATCGAAATGGGTGCTGCAATTTTACTTTAGTTCAAGCAGCTGTTGATGCAGTTCCAGCTCTTAGCCAGAAAAGAACAGTATTATGGATCAATACTGGCATATACTATGAAAAAGTCATTGTTCCAATCACCAAACCAAACATAACATTCCAAGGACAAGGGTATTTATCGACTGCAATATCATGGAATGACACGGCGAATTCATCACATGGCACATTCTACAGTGGCTCTGTTCAAGTTTTTGGTGCCAACTTCATTGCTAAGAACATAAGCTTCATGAATGTGGCTCCAATTCCAAAACCTGGTGATTTTGGAGCGCAAGCTGTAGCAATTAGGGTATCGGGGGACCAATCTGCATTTTGGGGTTGTGGATTCTTTGGAGCTCAAGACACCCTCCATGATGATAAGGGCCGCCATTACTTTAAGGAATGCTATATTCAGGGGTCCATTGATTTCATCTTTGGCAATGCAAGGTCATTCTATGAGAATTGCCAGTTGGTTTCGATGGCAAACCCAGTAGCCCCAGGATCAAAGAGCATTAGCGGAGCCCTTACAGCGCATGGCAGGGTTTCAAATGAGAACACTGGATATGCATTTGTGAACTGCTTTGTTGGAGGGTCTGGAAGGGTTTTGTTAGGTCGAGCATGGAGGCCTTTCTCACGTGTGGTGTTTATCAACACAACAATGACAGACATCATAGCTCCAGAAGGCTGGAGTGATTTCAATGACCCGACAAGAGACCAGACTATATTTTATGGAGAATACAACTGCTCAGGTGCAGGGGCTAATTTGACCATGCGGGCATCTTATGTACAGAGACTCAATGACACTCAAGCTTCTCCCTTCTTGAATGTGTCCTTCATTGATGGAAATGATTGGTTACAACCCATTAATGCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

421

Amino Acids

46.43

Weight (kDa)

8.89

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 121 - 412 1.1e-67 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 608
AccB7I CCANNNNNTGG 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 30
AciI CCGC 4 cut(s) 28, 775, 906, 1162
AclWI GGATC 3 cut(s) 372, 458, 898
AcsI RAATTY 1 cut(s) 562
AcvI CACGTG 1 cut(s) 1025
AfaI GTAC 1 cut(s) 1176
AfiI CCNNNNNNNGG 1 cut(s) 665
AflIII ACRYGT 1 cut(s) 1024
AgsI TTSAA 7 cut(s) 179, 204, 398, 599, 936, 1087, 1213
AjnI CCWGG 3 cut(s) 316, 664, 886
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 7 cut(s) 404, 422, 636, 686, 746, 1064, 1199
AluI AGCT 7 cut(s) 404, 422, 636, 686, 746, 1064, 1199
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 5 cut(s) 77, 194, 225, 1095, 1174
AlwI GGATC 3 cut(s) 372, 458, 898
AoxI GGCC 3 cut(s) 282, 772, 1013
ApeKI GCWGC 2 cut(s) 378, 401
ApoI RAATTY 1 cut(s) 562
AseI ATTAAT 1 cut(s) 1254
AspLEI GCGC 2 cut(s) 682, 922
AspS9I GGNCC 3 cut(s) 709, 772, 807
AsuHPI GGTGA 4 cut(s) 184, 201, 484, 680
AvaII GGWCC 2 cut(s) 709, 807
BanI GGYRCC 1 cut(s) 608
BanII GRGCYC 2 cut(s) 748, 913
BbrPI CACGTG 1 cut(s) 1025
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 2 cut(s) 365, 413
BccI CCATC 3 cut(s) 248, 862, 1223
BceAI ACGGC 1 cut(s) 573
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BciT130I CCWGG 3 cut(s) 318, 666, 888
BcoDI GTCTC 5 cut(s) 77, 194, 225, 1095, 1174
BfaI CTAG 2 cut(s) 255, 1264
BfmI CTRYAG 2 cut(s) 583, 687
BglII AGATCT 1 cut(s) 181
BisI GCNGC 3 cut(s) 379, 402, 775
BlsI GCNGC 3 cut(s) 380, 403, 776
Bme1390I CCNGG 3 cut(s) 318, 666, 888
Bme18I GGWCC 2 cut(s) 709, 807
BmgT120I GGNCC 3 cut(s) 709, 772, 807
BmiI GGNNCC 7 cut(s) 10, 26, 610, 651, 710, 808, 910
BmrFI CCNGG 3 cut(s) 318, 666, 888
BmrI ACTGGG 1 cut(s) 872
BmsI GCATC 3 cut(s) 268, 400, 1175
BmuI ACTGGG 1 cut(s) 872
BpmI CTGGAG 3 cut(s) 315, 1050, 1096
Bpu10I CCTNAGC 1 cut(s) 1135
BpuEI CTTGAG 2 cut(s) 732, 1179
BsaAI YACGTR 1 cut(s) 1025
BsaI GGTCTC 2 cut(s) 194, 1095
BsaJI CCNNGG 2 cut(s) 514, 886
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 665
Bse1I ACTGG 4 cut(s) 463, 856, 878, 952
Bse3DI GCAATG 2 cut(s) 618, 835
BseBI CCWGG 3 cut(s) 318, 666, 888
BseDI CCNNGG 2 cut(s) 514, 886
BseLI CCNNNNNNNGG 1 cut(s) 665
BseMI GCAATG 2 cut(s) 618, 835
BseMII CTCAG 1 cut(s) 1149
BseNI ACTGG 4 cut(s) 463, 856, 878, 952
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 2 cut(s) 365, 413
BsgI GTGCAG 1 cut(s) 1161
BshFI GGCC 3 cut(s) 284, 774, 1015
BshNI GGYRCC 1 cut(s) 608
BsiHKAI GWGCWC 1 cut(s) 748
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 1 cut(s) 665
BsmAI GTCTC 5 cut(s) 77, 194, 225, 1095, 1174
BsmFI GGGAC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 3 cut(s) 284, 774, 1015
Bso31I GGTCTC 2 cut(s) 194, 1095
Bsp1286I GDGCHC 2 cut(s) 748, 913
Bsp1407I TGTACA 1 cut(s) 1174
Bsp143I GATC 4 cut(s) 181, 364, 450, 890
BspACI CCGC 4 cut(s) 28, 775, 906, 1162
BspANI GGCC 3 cut(s) 284, 774, 1015
BspCNI CTCAG 1 cut(s) 1148
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 7 cut(s) 10, 26, 610, 651, 710, 808, 910
BspPI GGATC 3 cut(s) 372, 458, 898
BspT107I GGYRCC 1 cut(s) 608
BspTNI GGTCTC 2 cut(s) 194, 1095
BsrBI CCGCTC 1 cut(s) 30
BsrDI GCAATG 2 cut(s) 618, 835
BsrGI TGTACA 1 cut(s) 1174
BsrI ACTGG 4 cut(s) 463, 856, 878, 952
BssECI CCNNGG 2 cut(s) 514, 886
BssMI GATC 4 cut(s) 181, 364, 450, 890
BssT1I CCWWGG 1 cut(s) 514
Bst2UI CCWGG 3 cut(s) 318, 666, 888
Bst4CI ACNGT 2 cut(s) 442, 587
Bst6I CTCTTC 3 cut(s) 205, 228, 233
BstAUI TGTACA 1 cut(s) 1174
BstBAI YACGTR 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 684, 1164
BstDEI CTNAG 4 cut(s) 20, 425, 624, 1135
BstHHI GCGC 2 cut(s) 682, 922
BstKTI GATC 4 cut(s) 184, 367, 453, 893
BstMAI GTCTC 5 cut(s) 77, 194, 225, 1095, 1174
BstMBI GATC 4 cut(s) 181, 364, 450, 890
BstMWI GCNNNNNNNGC 5 cut(s) 110, 290, 410, 419, 917
BstNI CCWGG 3 cut(s) 318, 666, 888
BstNSI RCATGY 1 cut(s) 129
BstSCI CCNGG 3 cut(s) 316, 664, 886
BstSFI CTRYAG 2 cut(s) 583, 687
BstV1I GCAGC 2 cut(s) 365, 413
BstX2I RGATCY 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 1 cut(s) 181
BsuRI GGCC 3 cut(s) 284, 774, 1015
BtsIMutI CAGTG 2 cut(s) 592, 945
Cac8I GCNNGC 2 cut(s) 684, 1164
CciI TCATGA 1 cut(s) 639
CfoI GCGC 2 cut(s) 682, 922
Cfr13I GGNCC 3 cut(s) 709, 772, 807
CseI GACGC 1 cut(s) 282
CsiI ACCWGGT 1 cut(s) 664
Csp6I GTAC 1 cut(s) 1175
CviAII CATG 8 cut(s) 126, 546, 572, 640, 761, 923, 1008, 1159
CviQI GTAC 1 cut(s) 1175
DdeI CTNAG 4 cut(s) 20, 425, 624, 1135
DpnI GATC 4 cut(s) 183, 366, 452, 892
DpnII GATC 4 cut(s) 181, 364, 450, 890
Eam1104I CTCTTC 3 cut(s) 205, 228, 233
EarI CTCTTC 3 cut(s) 205, 228, 233
Ecl136II GAGCTC 1 cut(s) 746
Eco130I CCWWGG 1 cut(s) 514
Eco147I AGGCCT 1 cut(s) 1015
Eco24I GRGCYC 2 cut(s) 748, 913
Eco31I GGTCTC 2 cut(s) 194, 1095
Eco47I GGWCC 2 cut(s) 709, 807
Eco53kI GAGCTC 1 cut(s) 746
Eco72I CACGTG 1 cut(s) 1025
EcoICRI GAGCTC 1 cut(s) 746
EcoRI GAATTC 1 cut(s) 562
EcoRII CCWGG 3 cut(s) 316, 664, 886
EcoT14I CCWWGG 1 cut(s) 514
EcoT22I ATGCAT 1 cut(s) 958
EcoT38I GRGCYC 2 cut(s) 748, 913
ErhI CCWWGG 1 cut(s) 514
FaeI CATG 8 cut(s) 129, 549, 575, 643, 764, 926, 1011, 1162
FaqI GGGAC 1 cut(s) 722
FatI CATG 8 cut(s) 125, 545, 571, 639, 760, 922, 1007, 1158
FauI CCCGC 1 cut(s) 1155
Fnu4HI GCNGC 3 cut(s) 379, 402, 775
FriOI GRGCYC 2 cut(s) 748, 913
Fsp4HI GCNGC 3 cut(s) 379, 402, 775
FspBI CTAG 2 cut(s) 255, 1264
GlaI GCGC 2 cut(s) 681, 921
GluI GCNGC 3 cut(s) 379, 402, 775
GsuI CTGGAG 3 cut(s) 315, 1050, 1096
HaeIII GGCC 3 cut(s) 284, 774, 1015
HgaI GACGC 1 cut(s) 282
HhaI GCGC 2 cut(s) 682, 922
Hin1II CATG 8 cut(s) 129, 549, 575, 643, 764, 926, 1011, 1162
Hin6I GCGC 2 cut(s) 680, 920
HinP1I GCGC 2 cut(s) 680, 920
HindIII AAGCTT 2 cut(s) 634, 1197
HinfI GANTC 3 cut(s) 70, 735, 1182
HphI GGTGA 4 cut(s) 184, 201, 484, 680
Hpy166II GTNNAC 2 cut(s) 131, 964
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 6 cut(s) 332, 640, 749, 984, 1067, 1210
Hpy8I GTNNAC 2 cut(s) 131, 964
HpyAV CCTTC 4 cut(s) 981, 1064, 1216, 1231
HpyCH4III ACNGT 2 cut(s) 442, 587
HpyCH4IV ACGT 1 cut(s) 1024
HpyCH4V TGCA 8 cut(s) 113, 381, 413, 539, 719, 833, 956, 1142
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 290, 410, 419, 917
HpyF3I CTNAG 4 cut(s) 20, 425, 624, 1135
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 8 cut(s) 129, 549, 575, 643, 764, 926, 1011, 1162
HspAI GCGC 2 cut(s) 680, 920
Kzo9I GATC 4 cut(s) 181, 364, 450, 890
LmnI GCTCC 5 cut(s) 655, 677, 743, 908, 1069
Lsp1109I GCAGC 2 cut(s) 365, 413
LweI GCATC 3 cut(s) 268, 400, 1175
MabI ACCWGGT 1 cut(s) 664
MaeI CTAG 2 cut(s) 255, 1264
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 1 cut(s) 1242
MalI GATC 4 cut(s) 183, 366, 452, 892
MbiI CCGCTC 1 cut(s) 30
MboI GATC 4 cut(s) 181, 364, 450, 890
MboII GAAGA 5 cut(s) 36, 191, 192, 215, 220
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 2 cut(s) 748, 913
MluCI AATT 7 cut(s) 297, 382, 562, 654, 693, 850, 1150
MlyI GAGTC 2 cut(s) 79, 1176
MmeI TCCRAC 1 cut(s) 955
Mph1103I ATGCAT 1 cut(s) 958
MseI TTAA 5 cut(s) 54, 147, 249, 786, 1254
MslI CAYNNNNRTG 2 cut(s) 644, 1046
MspA1I CMGCKG 1 cut(s) 404
MspR9I CCNGG 3 cut(s) 318, 666, 888
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 3 cut(s) 318, 666, 888
MwoI GCNNNNNNNGC 5 cut(s) 110, 290, 410, 419, 917
NdeII GATC 4 cut(s) 181, 364, 450, 890
NlaIII CATG 8 cut(s) 129, 549, 575, 643, 764, 926, 1011, 1162
NlaIV GGNNCC 7 cut(s) 10, 26, 610, 651, 710, 808, 910
NsiI ATGCAT 1 cut(s) 958
NspI RCATGY 1 cut(s) 129
PagI TCATGA 1 cut(s) 639
PceI AGGCCT 1 cut(s) 1015
PctI GAATGC 1 cut(s) 797
PfeI GAWTC 1 cut(s) 735
PflMI CCANNNNNTGG 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 3 cut(s) 380, 403, 776
PleI GAGTC 2 cut(s) 78, 1176
PmaCI CACGTG 1 cut(s) 1025
PmlI CACGTG 1 cut(s) 1025
PpsI GAGTC 2 cut(s) 78, 1176
Ppu21I YACGTR 1 cut(s) 1025
PshBI ATTAAT 1 cut(s) 1254
Psp124BI GAGCTC 1 cut(s) 748
Psp6I CCWGG 3 cut(s) 316, 664, 886
PspCI CACGTG 1 cut(s) 1025
PspGI CCWGG 3 cut(s) 316, 664, 886
PspN4I GGNNCC 7 cut(s) 10, 26, 610, 651, 710, 808, 910
PspPI GGNCC 3 cut(s) 709, 772, 807
PsuI RGATCY 1 cut(s) 181
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 1 cut(s) 1176
RsaNI GTAC 1 cut(s) 1175
RseI CAYNNNNRTG 2 cut(s) 644, 1046
SacI GAGCTC 1 cut(s) 748
SaqAI TTAA 5 cut(s) 54, 147, 249, 786, 1254
SatI GCNGC 3 cut(s) 379, 402, 775
Sau3AI GATC 4 cut(s) 181, 364, 450, 890
Sau96I GGNCC 3 cut(s) 709, 772, 807
SchI GAGTC 2 cut(s) 79, 1176
ScrFI CCNGG 3 cut(s) 318, 666, 888
SduI GDGCHC 2 cut(s) 748, 913
SexAI ACCWGGT 1 cut(s) 664
SfaNI GCATC 3 cut(s) 268, 400, 1175
SfcI CTRYAG 2 cut(s) 583, 687
SinI GGWCC 2 cut(s) 709, 807
SmiMI CAYNNNNRTG 2 cut(s) 644, 1046
SmlI CTYRAG 2 cut(s) 747, 1194
SmoI CTYRAG 2 cut(s) 747, 1194
Sse9I AATT 7 cut(s) 297, 382, 562, 654, 693, 850, 1150
SseBI AGGCCT 1 cut(s) 1015
SsiI CCGC 4 cut(s) 28, 775, 906, 1162
SspMI CTAG 2 cut(s) 255, 1264
SstI GAGCTC 1 cut(s) 748
StuI AGGCCT 1 cut(s) 1015
StyD4I CCNGG 3 cut(s) 316, 664, 886
StyI CCWWGG 1 cut(s) 514
TaaI ACNGT 2 cut(s) 442, 587
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 4 cut(s) 367, 533, 866, 1003
TasI AATT 7 cut(s) 297, 382, 562, 654, 693, 850, 1150
TatI WGTACW 1 cut(s) 1174
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 1 cut(s) 735
Tru1I TTAA 5 cut(s) 54, 147, 249, 786, 1254
Tru9I TTAA 5 cut(s) 54, 147, 249, 786, 1254
TscAI CASTG 2 cut(s) 592, 952
TseI GCWGC 2 cut(s) 378, 401
TspDTI ATGAA 7 cut(s) 486, 555, 607, 628, 656, 808, 1213
TspRI CASTG 2 cut(s) 592, 952
Van91I CCANNNNNTGG 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 709, 807
VspI ATTAAT 1 cut(s) 1254
XapI RAATTY 1 cut(s) 562
XceI RCATGY 1 cut(s) 129
XspI CTAG 2 cut(s) 255, 1264
Zsp2I ATGCAT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.