Rh2BG513500

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012811
Physical Location & Seq
Reverse (-)
72163795 .. 72167442
3648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG513500.1

Sequence Viewer

Length: 1113 bp
ATGACGAGTCTCAAAAGCATATCCCTAACCTTTCTTGTTGCTATTTTTGCAGTTTTATGGGCATGTAAACATCTCATAGGTTTTAATCCAAACTCAAAATACCTCAAATACTTTGAAGATCTTGGTCTCACCACCTCTTCAATATCACCTATTTTGTCTCTTCTCTTCCCATACTCCATCAACTTTAATGCTAGGCATCACAAAAAGAAAGATAATAAGGCCAAAAAAGCGTCAATTTGTGATGACTTCCCTCCTGGAATACCTCCTCCAGATACCAACACAACCTCCTATTTTTGCGTGGATCGAAATGGGTGCTGCAATTTTACTTTAGTTCAAGCAGCTGTTGATGCAGTTCCAGCTCTTAGCCAGAAAAGAACAGTATTATGGATCAATACTGGCATATACTATGAAAAAGTCATTGTTCCAATCACCAAACCAAACATAACATTCCAAGGACAAGGGTATTTATCGACTGCAATATCATGGAATGACACGGCGAATTCATCACATGGCACATTCTACAGTGGCTCTGTTCAAGTTTTTGGTGCCAACTTCATTGCTAAGAACATAAGCTTCATGAATGTGGCTCCAATTCCAAAACCTGGTGATTTTGGAGCGCAAGCTGTAGCAATTAGGGTATCGGGGGACCAATCTGCATTTTGGGGTTGTGGATTCTTTGGAGCTCAAGACACCCTCCATGATGATAAGGGCCGCCATTACTTTAAGGAATGCTATATTCAGGGGTCCATTGATTTCATCTTTGGCAATGCAAGGTCATTCTATGAGTTGGTTTCGATGGCAAACCCAGTAGCCCCAGGATCAAAGAGCATTAGCGGAGCCCTTACAGCGCATGGCAGGGTTTCAAATGAGAACACTGGATATGCATTTGTGAACTGCTTTGTTGGAGGGTCTGGAAGGGTTTTGTTAGGTCGAGCATGGAGGCCTTTCTCACGTGTGGTGTTTATCAACACAACAATGACAGACATCATAGCTCCAGAAGGCTGGAGTGATTTCAATGACCCGACAAGAGACCAGTACGCTTCACTTCATAACATTGGCACTCTCCATGGCCTAAAGAACAGTAATGGGACTTCCTCATTGAAAGAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

370

Amino Acids

40.5

Weight (kDa)

8.9

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 100 - 342 1.1e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 602
AciI CCGC 2 cut(s) 712, 834
AclWI GGATC 3 cut(s) 309, 395, 826
AcsI RAATTY 1 cut(s) 499
AcvI CACGTG 1 cut(s) 953
AfaI GTAC 1 cut(s) 1037
AfiI CCNNNNNNNGG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 952
AgsI TTSAA 7 cut(s) 116, 141, 335, 536, 864, 1015, 1102
AjnI CCWGG 3 cut(s) 253, 601, 814
AluBI AGCT 6 cut(s) 341, 359, 573, 623, 683, 992
AluI AGCT 6 cut(s) 341, 359, 573, 623, 683, 992
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 4 cut(s) 14, 131, 162, 1023
AlwI GGATC 3 cut(s) 309, 395, 826
AoxI GGCC 4 cut(s) 219, 709, 941, 1069
ApeKI GCWGC 2 cut(s) 315, 338
ApoI RAATTY 1 cut(s) 499
Asp700I GAANNNNTTC 1 cut(s) 1106
AspLEI GCGC 2 cut(s) 619, 850
AspS9I GGNCC 3 cut(s) 646, 709, 744
AsuHPI GGTGA 4 cut(s) 121, 138, 421, 617
AvaII GGWCC 2 cut(s) 646, 744
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 2 cut(s) 685, 841
BarI GAAGNNNNNNTAC 2 cut(s) 1075, 1107
BbrPI CACGTG 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 2 cut(s) 302, 350
BccI CCATC 2 cut(s) 185, 790
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 3 cut(s) 255, 603, 816
BcoDI GTCTC 4 cut(s) 14, 131, 162, 1023
BfaI CTAG 2 cut(s) 192, 1111
BfmI CTRYAG 2 cut(s) 520, 624
BglII AGATCT 1 cut(s) 118
BisI GCNGC 3 cut(s) 316, 339, 712
BlsI GCNGC 3 cut(s) 317, 340, 713
Bme1390I CCNGG 3 cut(s) 255, 603, 816
Bme18I GGWCC 2 cut(s) 646, 744
BmgT120I GGNCC 3 cut(s) 646, 709, 744
BmiI GGNNCC 5 cut(s) 547, 588, 647, 745, 838
BmrFI CCNGG 3 cut(s) 255, 603, 816
BmrI ACTGGG 1 cut(s) 800
BmsI GCATC 2 cut(s) 205, 337
BmuI ACTGGG 1 cut(s) 800
BpmI CTGGAG 3 cut(s) 252, 978, 1024
BpuEI CTTGAG 1 cut(s) 669
BsaAI YACGTR 1 cut(s) 953
BsaI GGTCTC 2 cut(s) 131, 1023
BsaJI CCNNGG 3 cut(s) 451, 814, 1066
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 400, 806, 880, 1033
Bse3DI GCAATG 2 cut(s) 555, 772
BseBI CCWGG 3 cut(s) 255, 603, 816
BseDI CCNNGG 3 cut(s) 451, 814, 1066
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMI GCAATG 2 cut(s) 555, 772
BseNI ACTGG 4 cut(s) 400, 806, 880, 1033
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 302, 350
BshFI GGCC 4 cut(s) 221, 711, 943, 1071
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 685
BslFI GGGAC 2 cut(s) 659, 1102
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 4 cut(s) 14, 131, 162, 1023
BsmFI GGGAC 2 cut(s) 659, 1102
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 4 cut(s) 221, 711, 943, 1071
Bso31I GGTCTC 2 cut(s) 131, 1023
Bsp1286I GDGCHC 2 cut(s) 685, 841
Bsp143I GATC 4 cut(s) 118, 301, 387, 818
Bsp19I CCATGG 1 cut(s) 1066
BspACI CCGC 2 cut(s) 712, 834
BspANI GGCC 4 cut(s) 221, 711, 943, 1071
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 5 cut(s) 547, 588, 647, 745, 838
BspPI GGATC 3 cut(s) 309, 395, 826
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 2 cut(s) 131, 1023
BsrDI GCAATG 2 cut(s) 555, 772
BsrI ACTGG 4 cut(s) 400, 806, 880, 1033
BssECI CCNNGG 3 cut(s) 451, 814, 1066
BssMI GATC 4 cut(s) 118, 301, 387, 818
BssT1I CCWWGG 2 cut(s) 451, 1066
Bst2UI CCWGG 3 cut(s) 255, 603, 816
Bst4CI ACNGT 3 cut(s) 379, 524, 1082
Bst6I CTCTTC 3 cut(s) 142, 165, 170
BstBAI YACGTR 1 cut(s) 953
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 2 cut(s) 362, 561
BstDSI CCRYGG 1 cut(s) 1066
BstHHI GCGC 2 cut(s) 619, 850
BstKTI GATC 4 cut(s) 121, 304, 390, 821
BstMAI GTCTC 4 cut(s) 14, 131, 162, 1023
BstMBI GATC 4 cut(s) 118, 301, 387, 818
BstMWI GCNNNNNNNGC 5 cut(s) 47, 227, 347, 356, 845
BstNI CCWGG 3 cut(s) 255, 603, 816
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 3 cut(s) 253, 601, 814
BstSFI CTRYAG 2 cut(s) 520, 624
BstV1I GCAGC 2 cut(s) 302, 350
BstX2I RGATCY 1 cut(s) 118
BstXI CCANNNNNNTGG 1 cut(s) 1002
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 4 cut(s) 221, 711, 943, 1071
BtgI CCRYGG 1 cut(s) 1066
BtsIMutI CAGTG 2 cut(s) 529, 873
Cac8I GCNNGC 1 cut(s) 621
CciI TCATGA 1 cut(s) 576
CfoI GCGC 2 cut(s) 619, 850
Cfr13I GGNCC 3 cut(s) 646, 709, 744
CseI GACGC 1 cut(s) 219
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 1 cut(s) 1036
CviAII CATG 8 cut(s) 63, 483, 509, 577, 698, 851, 936, 1067
CviQI GTAC 1 cut(s) 1036
DdeI CTNAG 2 cut(s) 362, 561
DpnI GATC 4 cut(s) 120, 303, 389, 820
DpnII GATC 4 cut(s) 118, 301, 387, 818
Eam1104I CTCTTC 3 cut(s) 142, 165, 170
EarI CTCTTC 3 cut(s) 142, 165, 170
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 2 cut(s) 451, 1066
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 685, 841
Eco31I GGTCTC 2 cut(s) 131, 1023
Eco47I GGWCC 2 cut(s) 646, 744
Eco53kI GAGCTC 1 cut(s) 683
Eco72I CACGTG 1 cut(s) 953
EcoICRI GAGCTC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 253, 601, 814
EcoT14I CCWWGG 2 cut(s) 451, 1066
EcoT22I ATGCAT 1 cut(s) 886
EcoT38I GRGCYC 2 cut(s) 685, 841
ErhI CCWWGG 2 cut(s) 451, 1066
FaeI CATG 8 cut(s) 66, 486, 512, 580, 701, 854, 939, 1070
FaqI GGGAC 2 cut(s) 659, 1102
FatI CATG 8 cut(s) 62, 482, 508, 576, 697, 850, 935, 1066
Fnu4HI GCNGC 3 cut(s) 316, 339, 712
FriOI GRGCYC 2 cut(s) 685, 841
Fsp4HI GCNGC 3 cut(s) 316, 339, 712
FspBI CTAG 2 cut(s) 192, 1111
GlaI GCGC 2 cut(s) 618, 849
GluI GCNGC 3 cut(s) 316, 339, 712
GsuI CTGGAG 3 cut(s) 252, 978, 1024
HaeIII GGCC 4 cut(s) 221, 711, 943, 1071
HgaI GACGC 1 cut(s) 219
HhaI GCGC 2 cut(s) 619, 850
Hin1II CATG 8 cut(s) 66, 486, 512, 580, 701, 854, 939, 1070
Hin6I GCGC 2 cut(s) 617, 848
HinP1I GCGC 2 cut(s) 617, 848
HindIII AAGCTT 1 cut(s) 571
HinfI GANTC 2 cut(s) 7, 672
HphI GGTGA 4 cut(s) 121, 138, 421, 617
Hpy166II GTNNAC 2 cut(s) 68, 892
Hpy188III TCNNGA 5 cut(s) 269, 577, 686, 912, 995
Hpy8I GTNNAC 2 cut(s) 68, 892
HpyAV CCTTC 2 cut(s) 909, 992
HpyCH4III ACNGT 3 cut(s) 379, 524, 1082
HpyCH4IV ACGT 1 cut(s) 952
HpyCH4V TGCA 7 cut(s) 50, 318, 350, 476, 656, 770, 884
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 227, 347, 356, 845
HpyF3I CTNAG 2 cut(s) 362, 561
HpySE526I ACGT 1 cut(s) 952
Hsp92II CATG 8 cut(s) 66, 486, 512, 580, 701, 854, 939, 1070
HspAI GCGC 2 cut(s) 617, 848
Kzo9I GATC 4 cut(s) 118, 301, 387, 818
LmnI GCTCC 5 cut(s) 592, 614, 680, 836, 997
Lsp1109I GCAGC 2 cut(s) 302, 350
LweI GCATC 2 cut(s) 205, 337
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 2 cut(s) 192, 1111
MaeII ACGT 1 cut(s) 952
MalI GATC 4 cut(s) 120, 303, 389, 820
MboI GATC 4 cut(s) 118, 301, 387, 818
MboII GAAGA 4 cut(s) 128, 129, 152, 157
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 2 cut(s) 685, 841
MluCI AATT 5 cut(s) 234, 319, 499, 591, 630
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 883
Mph1103I ATGCAT 1 cut(s) 886
MroXI GAANNNNTTC 1 cut(s) 1106
MseI TTAA 3 cut(s) 84, 186, 723
MslI CAYNNNNRTG 2 cut(s) 581, 974
MspA1I CMGCKG 1 cut(s) 341
MspR9I CCNGG 3 cut(s) 255, 603, 816
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 3 cut(s) 255, 603, 816
MwoI GCNNNNNNNGC 5 cut(s) 47, 227, 347, 356, 845
NcoI CCATGG 1 cut(s) 1066
NdeII GATC 4 cut(s) 118, 301, 387, 818
NlaIII CATG 8 cut(s) 66, 486, 512, 580, 701, 854, 939, 1070
NlaIV GGNNCC 5 cut(s) 547, 588, 647, 745, 838
NsiI ATGCAT 1 cut(s) 886
NspI RCATGY 1 cut(s) 66
PagI TCATGA 1 cut(s) 576
PceI AGGCCT 1 cut(s) 943
PctI GAATGC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 1106
PfeI GAWTC 1 cut(s) 672
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 3 cut(s) 317, 340, 713
PleI GAGTC 1 cut(s) 15
PmaCI CACGTG 1 cut(s) 953
PmlI CACGTG 1 cut(s) 953
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 253, 601, 814
PspCI CACGTG 1 cut(s) 953
PspGI CCWGG 3 cut(s) 253, 601, 814
PspN4I GGNNCC 5 cut(s) 547, 588, 647, 745, 838
PspPI GGNCC 3 cut(s) 646, 709, 744
PsuI RGATCY 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 1037
RsaNI GTAC 1 cut(s) 1036
RseI CAYNNNNRTG 2 cut(s) 581, 974
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 3 cut(s) 84, 186, 723
SatI GCNGC 3 cut(s) 316, 339, 712
Sau3AI GATC 4 cut(s) 118, 301, 387, 818
Sau96I GGNCC 3 cut(s) 646, 709, 744
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 3 cut(s) 255, 603, 816
SduI GDGCHC 2 cut(s) 685, 841
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 205, 337
SfcI CTRYAG 2 cut(s) 520, 624
SinI GGWCC 2 cut(s) 646, 744
SmiMI CAYNNNNRTG 2 cut(s) 581, 974
SmlI CTYRAG 1 cut(s) 684
SmoI CTYRAG 1 cut(s) 684
Sse9I AATT 5 cut(s) 234, 319, 499, 591, 630
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 2 cut(s) 712, 834
SspMI CTAG 2 cut(s) 192, 1111
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 3 cut(s) 253, 601, 814
StyI CCWWGG 2 cut(s) 451, 1066
TaaI ACNGT 3 cut(s) 379, 524, 1082
TaiI ACGT 1 cut(s) 955
TaqI TCGA 4 cut(s) 304, 470, 794, 931
TasI AATT 5 cut(s) 234, 319, 499, 591, 630
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 672
Tru1I TTAA 3 cut(s) 84, 186, 723
Tru9I TTAA 3 cut(s) 84, 186, 723
TscAI CASTG 2 cut(s) 529, 880
TseI GCWGC 2 cut(s) 315, 338
TspDTI ATGAA 7 cut(s) 423, 492, 544, 565, 593, 745, 1037
TspRI CASTG 2 cut(s) 529, 880
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 646, 744
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 1106
XspI CTAG 2 cut(s) 192, 1111
Zsp2I ATGCAT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.