MD09G1139900.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012811
Physical Location & Seq
Forward (+)
10880349 .. 10882764
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1139900.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGAGTCTCAAAACCACATTCCTAACATATTTTTTTGCCATTTTTGCAATTTTATTGGCATCTCGAAGTCTCATAAGTATTCCAAAATCGTCATCCCTCAAATACTTTGTCAACCTAAGTGATCTCACCACCTCATCCTCAGTGCCCAATATTATTTCTTTTCTCATCCCACAAAGCATATTTGCTAGGCATCACAAAAAACATGAGGATGGGGGAAAAATAGGATCCATTTGTGATGATTTTCCACGTGGTTTTCCTCCTCCAGACACCAACACAACCTCCTACCTTTGTGTTGATCGAAATGGGTGTTGCAATTTCACATTGGTTCAAGCAGCTGTTGATGCAGTTCCAACTCCTAGCCCGAAGAGAACAATAATTTGGATTAATAAGGGCATTTACTATGAGAAAGTCACGGTTCCAAAAACCAAACCAAATATAACATTTCAAGGACAAGGGTATGAAACGACCGCAATATCATGGAACGACACAGCCAATTCAGCGCATGGCACATTTTACAGTGGCTCAGTTCAAGTTTTTGCTGACAACTTTGTTGCCAAGAACATAAGCTTCATGAATGTGGCTCCAATTCCGAAGCCTGGAGATGTGGGAGCTCAAGCTGTAGCGATTAGAATATCAGGAGACCAAGCAGCATTCTTGGGTTGTGGGTTCTTTGGAGCTCAAGACACCCTTCACGATGATAGGGGCCGCCATTACTTCAAGGATTGCTATATCCAGGGCTCCATTGATTTCATCTTTGGCAATGGAAGGTCATTGTATGAGAATTGCCAATTGATTTCGATGTCAAACCCAGTACCCCTAGGAACAAAGAGCATAAACGGCGCGGTTACAGCGCACGGCAGAGCTTCCAAGGATGACAACACTGGCTATGCATTTGTCAACTGCTTCATTGGAGGGACTGGAAGAGTATGGCTGGGTCGAGCATGGAGGCCTTTCTCTCGAGTGGTTTTTGCCAGCACTACGATGACAGACATTATAGCTCCTGAAGGGTGGAATGACTTCAATGATCCAACAAGAGACCAGACTATATTCTATGGAGAATACAATTGCTCAGGTGCAGGAGCAAATATGAGCTTGCGTGCGCCTTATGTACAGAAGCTCAATGACACACAAGCTTCTCTTTTCCTTAATGTGTCTTTCATTGATGGGGGAGATTGGCTGCAATCCATTATTAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

400

Amino Acids

43.75

Weight (kDa)

8.01

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 98 - 389 1.7e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 842
AciI CCGC 3 cut(s) 468, 706, 842
AclWI GGATC 3 cut(s) 219, 232, 1019
AcuI CTGAAG 1 cut(s) 1023
AcvI CACGTG 1 cut(s) 248
AfaI GTAC 2 cut(s) 813, 1110
AfiI CCNNNNNNNGG 1 cut(s) 596
AgsI TTSAA 5 cut(s) 329, 446, 530, 718, 1021
AjnI CCWGG 2 cut(s) 595, 732
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
Alw21I GWGCWC 2 cut(s) 613, 679
Alw26I GTCTC 4 cut(s) 11, 74, 633, 1029
AlwI GGATC 3 cut(s) 219, 232, 1019
Ama87I CYCGRG 1 cut(s) 957
AoxI GGCC 2 cut(s) 703, 947
ApeKI GCWGC 3 cut(s) 332, 647, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
AseI ATTAAT 2 cut(s) 384, 1191
Asp700I GAANNNNTTC 1 cut(s) 1016
AspA2I CCTAGG 1 cut(s) 817
AspLEI GCGC 4 cut(s) 502, 842, 853, 1102
AspS9I GGNCC 1 cut(s) 703
AsuHPI GGTGA 1 cut(s) 118
AvaI CYCGRG 1 cut(s) 957
AvrII CCTAGG 1 cut(s) 817
BaeGI GKGCMC 1 cut(s) 147
BamHI GGATCC 1 cut(s) 224
BanII GRGCYC 3 cut(s) 613, 679, 740
BbrPI CACGTG 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 613, 679
BbvI GCAGC 3 cut(s) 344, 659, 1164
BccI CCATC 2 cut(s) 203, 1157
BceAI ACGGC 2 cut(s) 853, 871
BciT130I CCWGG 2 cut(s) 597, 734
BcoDI GTCTC 4 cut(s) 11, 74, 633, 1029
BfaI CTAG 3 cut(s) 186, 357, 818
BfmI CTRYAG 1 cut(s) 618
BisI GCNGC 4 cut(s) 333, 648, 706, 1178
BlnI CCTAGG 1 cut(s) 817
BlsI GCNGC 4 cut(s) 334, 649, 707, 1179
Bme1390I CCNGG 2 cut(s) 597, 734
BmeT110I CYCGRG 1 cut(s) 957
BmgT120I GGNCC 1 cut(s) 703
BmiI GGNNCC 5 cut(s) 226, 417, 582, 704, 739
BmrFI CCNGG 2 cut(s) 597, 734
BmrI ACTGGG 1 cut(s) 803
BmsI GCATC 3 cut(s) 68, 199, 331
BmuI ACTGGG 1 cut(s) 803
BpmI CTGGAG 2 cut(s) 246, 618
Bpu10I CCTNAGC 1 cut(s) 1069
BpuEI CTTGAG 2 cut(s) 597, 663
BsaAI YACGTR 1 cut(s) 248
BsaI GGTCTC 2 cut(s) 633, 1029
BsaJI CCNNGG 3 cut(s) 733, 817, 867
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse1I ACTGG 3 cut(s) 809, 886, 922
Bse3DI GCAATG 1 cut(s) 766
BseBI CCWGG 2 cut(s) 597, 734
BseDI CCNNGG 3 cut(s) 733, 817, 867
BseGI GGATG 5 cut(s) 92, 134, 165, 214, 877
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 3 cut(s) 153, 537, 1083
BseNI ACTGG 3 cut(s) 809, 886, 922
BseRI GAGGAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 3 cut(s) 344, 659, 1164
BseYI CCCAGC 1 cut(s) 931
BsgI GTGCAG 1 cut(s) 1095
Bsh1236I CGCG 1 cut(s) 842
Bsh1285I CGRYCG 1 cut(s) 468
BshFI GGCC 2 cut(s) 705, 949
BsiEI CGRYCG 1 cut(s) 468
BsiHKAI GWGCWC 2 cut(s) 613, 679
BsiHKCI CYCGRG 1 cut(s) 957
BslFI GGGAC 1 cut(s) 928
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 4 cut(s) 11, 74, 633, 1029
BsmFI GGGAC 1 cut(s) 928
BsmI GAATGC 1 cut(s) 650
BsnI GGCC 2 cut(s) 705, 949
Bso31I GGTCTC 2 cut(s) 633, 1029
BsoBI CYCGRG 1 cut(s) 957
Bsp1286I GDGCHC 4 cut(s) 147, 613, 679, 740
Bsp1407I TGTACA 1 cut(s) 1108
Bsp143I GATC 4 cut(s) 121, 224, 295, 1024
BspACI CCGC 3 cut(s) 468, 706, 842
BspANI GGCC 2 cut(s) 705, 949
BspCNI CTCAG 3 cut(s) 152, 536, 1082
BspFNI CGCG 1 cut(s) 842
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 5 cut(s) 226, 417, 582, 704, 739
BspPI GGATC 3 cut(s) 219, 232, 1019
BspTNI GGTCTC 2 cut(s) 633, 1029
BsrDI GCAATG 1 cut(s) 766
BsrGI TGTACA 1 cut(s) 1108
BsrI ACTGG 3 cut(s) 809, 886, 922
BssECI CCNNGG 3 cut(s) 733, 817, 867
BssMI GATC 4 cut(s) 121, 224, 295, 1024
BssT1I CCWWGG 2 cut(s) 817, 867
Bst2UI CCWGG 2 cut(s) 597, 734
Bst4CI ACNGT 2 cut(s) 415, 518
Bst6I CTCTTC 2 cut(s) 359, 916
BstAUI TGTACA 1 cut(s) 1108
BstBAI YACGTR 1 cut(s) 248
BstC8I GCNNGC 3 cut(s) 973, 1094, 1098
BstDEI CTNAG 4 cut(s) 116, 139, 523, 1069
BstF5I GGATG 5 cut(s) 92, 134, 165, 214, 877
BstFNI CGCG 1 cut(s) 842
BstHHI GCGC 4 cut(s) 502, 842, 853, 1102
BstKTI GATC 4 cut(s) 124, 227, 298, 1027
BstMAI GTCTC 4 cut(s) 11, 74, 633, 1029
BstMBI GATC 4 cut(s) 121, 224, 295, 1024
BstMCI CGRYCG 1 cut(s) 468
BstMWI GCNNNNNNNGC 5 cut(s) 44, 341, 497, 837, 848
BstNI CCWGG 2 cut(s) 597, 734
BstSCI CCNGG 2 cut(s) 595, 732
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 1 cut(s) 147
BstUI CGCG 1 cut(s) 842
BstV1I GCAGC 3 cut(s) 344, 659, 1164
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BsuRI GGCC 2 cut(s) 705, 949
BtsCI GGATG 5 cut(s) 92, 134, 165, 214, 877
BtsIMutI CAGTG 3 cut(s) 147, 523, 879
Cac8I GCNNGC 3 cut(s) 973, 1094, 1098
CciI TCATGA 1 cut(s) 570
CfoI GCGC 4 cut(s) 502, 842, 853, 1102
Cfr13I GGNCC 1 cut(s) 703
Csp6I GTAC 2 cut(s) 812, 1109
CviAII CATG 5 cut(s) 203, 477, 503, 571, 942
CviQI GTAC 2 cut(s) 812, 1109
DdeI CTNAG 4 cut(s) 116, 139, 523, 1069
DpnI GATC 4 cut(s) 123, 226, 297, 1026
DpnII GATC 4 cut(s) 121, 224, 295, 1024
Eam1104I CTCTTC 2 cut(s) 359, 916
EarI CTCTTC 2 cut(s) 359, 916
Ecl136II GAGCTC 2 cut(s) 611, 677
Eco130I CCWWGG 2 cut(s) 817, 867
Eco147I AGGCCT 1 cut(s) 949
Eco24I GRGCYC 3 cut(s) 613, 679, 740
Eco31I GGTCTC 2 cut(s) 633, 1029
Eco53kI GAGCTC 2 cut(s) 611, 677
Eco57I CTGAAG 1 cut(s) 1023
Eco72I CACGTG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 957
EcoICRI GAGCTC 2 cut(s) 611, 677
EcoRII CCWGG 2 cut(s) 595, 732
EcoT14I CCWWGG 2 cut(s) 817, 867
EcoT22I ATGCAT 1 cut(s) 892
EcoT38I GRGCYC 3 cut(s) 613, 679, 740
ErhI CCWWGG 2 cut(s) 817, 867
FaeI CATG 5 cut(s) 206, 480, 506, 574, 945
FalI AAGNNNNNCTT 4 cut(s) 672, 704, 1122, 1154
FaqI GGGAC 1 cut(s) 928
FatI CATG 5 cut(s) 202, 476, 502, 570, 941
Fnu4HI GCNGC 4 cut(s) 333, 648, 706, 1178
FokI GGATG 5 cut(s) 79, 121, 152, 221, 884
FriOI GRGCYC 3 cut(s) 613, 679, 740
Fsp4HI GCNGC 4 cut(s) 333, 648, 706, 1178
FspBI CTAG 3 cut(s) 186, 357, 818
GlaI GCGC 4 cut(s) 501, 841, 852, 1101
GluI GCNGC 4 cut(s) 333, 648, 706, 1178
GsaI CCCAGC 1 cut(s) 935
GsuI CTGGAG 2 cut(s) 246, 618
HaeIII GGCC 2 cut(s) 705, 949
HhaI GCGC 4 cut(s) 502, 842, 853, 1102
Hin1II CATG 5 cut(s) 206, 480, 506, 574, 945
Hin6I GCGC 4 cut(s) 500, 840, 851, 1100
HinP1I GCGC 4 cut(s) 500, 840, 851, 1100
HincII GTYRAC 2 cut(s) 112, 898
HindII GTYRAC 2 cut(s) 112, 898
HindIII AAGCTT 2 cut(s) 565, 1131
HinfI GANTC 1 cut(s) 4
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 2 cut(s) 112, 898
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 8 cut(s) 63, 263, 571, 636, 680, 692, 957, 1001
Hpy8I GTNNAC 2 cut(s) 112, 898
HpyAV CCTTC 3 cut(s) 698, 759, 998
HpyCH4III ACNGT 2 cut(s) 415, 518
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 6 cut(s) 47, 312, 344, 890, 1076, 1180
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 341, 497, 837, 848
HpyF3I CTNAG 4 cut(s) 116, 139, 523, 1069
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 5 cut(s) 206, 480, 506, 574, 945
HspAI GCGC 4 cut(s) 500, 840, 851, 1100
Kzo9I GATC 4 cut(s) 121, 224, 295, 1024
LmnI GCTCC 6 cut(s) 586, 608, 674, 743, 1003, 1079
Lsp1109I GCAGC 3 cut(s) 344, 659, 1164
LweI GCATC 3 cut(s) 68, 199, 331
MaeI CTAG 3 cut(s) 186, 357, 818
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 2 cut(s) 409, 844
MalI GATC 4 cut(s) 123, 226, 297, 1026
MboI GATC 4 cut(s) 121, 224, 295, 1024
MboII GAAGA 2 cut(s) 376, 933
MfeI CAATTG 2 cut(s) 788, 1063
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 4 cut(s) 147, 613, 679, 740
MluCI AATT 9 cut(s) 48, 313, 375, 493, 585, 781, 788, 1063, 1192
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 374, 1052
MnlI CCTC 9 cut(s) 107, 142, 148, 199, 267, 270, 289, 905, 939
Mph1103I ATGCAT 1 cut(s) 892
MroXI GAANNNNTTC 1 cut(s) 1016
MseI TTAA 4 cut(s) 384, 1146, 1191, 1198
MslI CAYNNNNRTG 3 cut(s) 207, 575, 980
MspA1I CMGCKG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 597, 734
MunI CAATTG 2 cut(s) 788, 1063
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 2 cut(s) 597, 734
MvnI CGCG 1 cut(s) 842
MwoI GCNNNNNNNGC 5 cut(s) 44, 341, 497, 837, 848
NdeII GATC 4 cut(s) 121, 224, 295, 1024
NlaIII CATG 5 cut(s) 206, 480, 506, 574, 945
NlaIV GGNNCC 5 cut(s) 226, 417, 582, 704, 739
NmuCI GTSAC 1 cut(s) 409
NsiI ATGCAT 1 cut(s) 892
PaeR7I CTCGAG 1 cut(s) 957
PagI TCATGA 1 cut(s) 570
PceI AGGCCT 1 cut(s) 949
PctI GAATGC 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 1016
PkrI GCNGC 4 cut(s) 334, 649, 707, 1179
PleI GAGTC 1 cut(s) 12
PmaCI CACGTG 1 cut(s) 248
PmlI CACGTG 1 cut(s) 248
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 248
PshBI ATTAAT 2 cut(s) 384, 1191
Psp124BI GAGCTC 2 cut(s) 613, 679
Psp6I CCWGG 2 cut(s) 595, 732
PspCI CACGTG 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 931
PspGI CCWGG 2 cut(s) 595, 732
PspN4I GGNNCC 5 cut(s) 226, 417, 582, 704, 739
PspPI GGNCC 1 cut(s) 703
PsuI RGATCY 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 2 cut(s) 813, 1110
RsaNI GTAC 2 cut(s) 812, 1109
RseI CAYNNNNRTG 3 cut(s) 207, 575, 980
SacI GAGCTC 2 cut(s) 613, 679
SaqAI TTAA 4 cut(s) 384, 1146, 1191, 1198
SatI GCNGC 4 cut(s) 333, 648, 706, 1178
Sau3AI GATC 4 cut(s) 121, 224, 295, 1024
Sau96I GGNCC 1 cut(s) 703
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 597, 734
SduI GDGCHC 4 cut(s) 147, 613, 679, 740
SfaNI GCATC 3 cut(s) 68, 199, 331
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 957
SlaI CTCGAG 1 cut(s) 957
SmiMI CAYNNNNRTG 3 cut(s) 207, 575, 980
SmlI CTYRAG 3 cut(s) 612, 678, 957
SmoI CTYRAG 3 cut(s) 612, 678, 957
Sse9I AATT 9 cut(s) 48, 313, 375, 493, 585, 781, 788, 1063, 1192
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 3 cut(s) 468, 706, 842
SspI AATATT 1 cut(s) 151
SspMI CTAG 3 cut(s) 186, 357, 818
SstI GAGCTC 2 cut(s) 613, 679
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 2 cut(s) 595, 732
StyI CCWWGG 2 cut(s) 817, 867
TaaI ACNGT 2 cut(s) 415, 518
TaiI ACGT 1 cut(s) 250
TaqI TCGA 5 cut(s) 64, 298, 797, 937, 958
TasI AATT 9 cut(s) 48, 313, 375, 493, 585, 781, 788, 1063, 1192
TatI WGTACW 1 cut(s) 1108
TauI GCSGC 1 cut(s) 708
Tru1I TTAA 4 cut(s) 384, 1146, 1191, 1198
Tru9I TTAA 4 cut(s) 384, 1146, 1191, 1198
TscAI CASTG 3 cut(s) 147, 523, 886
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 3 cut(s) 332, 647, 1177
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 6 cut(s) 474, 559, 587, 739, 895, 1147
TspRI CASTG 3 cut(s) 147, 523, 886
VspI ATTAAT 2 cut(s) 384, 1191
XhoI CTCGAG 1 cut(s) 957
XmaJI CCTAGG 1 cut(s) 817
XmnI GAANNNNTTC 1 cut(s) 1016
XspI CTAG 3 cut(s) 186, 357, 818
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.