AT1G10830

NnrU protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010250
Physical Location & Seq
Reverse (-)
3605512 .. 3607580
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10830.1

Sequence Viewer

Length: 1104 bp
ATGGCGGTTTATCATCTCCTTCTCTCGAGCCCACCTTCGCTTCTTCTTCTCCCTCCTTCTCCTCGCCGCCCTAACCTCACCTTAATCCGCCGTATCCCAGCTCATCCTCGCCTCGGAAACTCGACCAGCTTGCTTTCTTCTTCGTCACCGGTAATTCGGAAAATTCTGGTTCGTAGCACTCTCCGGGAAGACCAACCCATCGCCTCTGACTCCGAGAGTAGTCCTACTCTACTGATCGGTGAAGATTCTGCTGCTTTTGAATTAGGGAAACAGAAGCTTGTTTCCTGGGTTTATTTCGGTGTTGTCCTTGGAGTTGTTCTCTTTATCCTCAATGTTGTTTGGATCGATAATTCCACCGGTTTCGGAAAATCATTCATCGACGCCGTCTCTAACATCTCCGGCTCACCGGAGGTTGCTATGTTAATGCTTATCCTTATATTTGCAATTGTACATAGTGGTTTAGCTAGTCTAAGGGATATAGGAGAGAAACTTATAGGTGAACGAGCATTTCGTGTTTTGTTTGCTGGAATCTCTCTTCCTTTAGCTATGAGCACCATTGTTTACTTTATAAACCATAGATATGATGGGTCACAGTTATGGCAGCTTCAGGGTGTTCCTGGAGTCCATGAAGCTATTTGGGTCGCTAATTTTGTATCCTTCTTCTTTCTCTATCCTTCAACCTTCAATCTTCTGGAGGTAGCAGCTGTTGACAAACCGAAGATGCATCTCTGGGAGACCGGCATCATGAGAATCACTCGACATCCTCAGATGGTTGGACAAATCGTTTGGTGTTTAGCACATACTCTCTGGATCGGAAACACAGTAGCTGCATCAGCTTCTCTCGGTTTGATTGCACACCATTTGTTTGGGGCGTGGAATGGAGACAGGAGATTGGCAAAAAGATATGGAGAGGATTTTGAAAGCATCAAGAAGAGAACAAGTGTGATTCCATTTGCTGCCATTTTCGAAGGACGGCAAGTTTTACCTGAGGATTACTACAAAGAATTTGTCAGGTTGCCATATCTTGCAATCACTGCGTTAACGGTTGGGGCATATTTTGCTCACCCGCTAATGCAAGGTGCAAGCTTTAGGCTTCATTGGTAA

Protein Analysis

367

Amino Acids

40.85

Weight (kDa)

9.4

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NnrU PF07298 140 - 361 4.6e-37 NnrU protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AciI CCGC 4 cut(s) 5, 67, 88, 1067
AclWI GGATC 2 cut(s) 350, 818
AcsI RAATTY 2 cut(s) 162, 1004
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 450
AfiI CCNNNNNNNGG 1 cut(s) 113
AgeI ACCGGT 2 cut(s) 148, 356
AgsI TTSAA 4 cut(s) 260, 678, 685, 920
AjnI CCWGG 2 cut(s) 284, 616
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 3 cut(s) 391, 728, 876
AlwI GGATC 2 cut(s) 350, 818
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 1 cut(s) 25
ApeKI GCWGC 5 cut(s) 251, 601, 701, 827, 956
ApoI RAATTY 2 cut(s) 162, 1004
ArsI GACNNNNNNTTYG 4 cut(s) 768, 800, 993, 1025
AsiGI ACCGGT 2 cut(s) 148, 356
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 6 cut(s) 70, 138, 251, 396, 509, 1055
AsuII TTCGAA 1 cut(s) 966
AvaI CYCGRG 1 cut(s) 25
AxyI CCTNAGG 1 cut(s) 987
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 5 cut(s) 238, 613, 713, 814, 943
BccI CCATC 3 cut(s) 206, 578, 763
BceAI ACGGC 3 cut(s) 75, 368, 989
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 2 cut(s) 286, 618
BciVI GTATCC 2 cut(s) 104, 664
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 3 cut(s) 391, 728, 876
BfaI CTAG 1 cut(s) 465
BfuI GTATCC 2 cut(s) 104, 664
BisI GCNGC 6 cut(s) 67, 252, 602, 702, 828, 957
BlsI GCNGC 6 cut(s) 68, 253, 603, 703, 829, 958
Bme1390I CCNGG 3 cut(s) 185, 286, 618
BmeT110I CYCGRG 1 cut(s) 25
BmrFI CCNGG 3 cut(s) 185, 286, 618
BmsI GCATC 5 cut(s) 711, 733, 750, 839, 933
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 4 cut(s) 303, 335, 739, 771
BpmI CTGGAG 2 cut(s) 639, 713
Bpu14I TTCGAA 1 cut(s) 966
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 3 cut(s) 112, 285, 307
BsaWI WCCGGW 3 cut(s) 148, 356, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 113
Bse118I RCCGGY 3 cut(s) 148, 356, 737
Bse21I CCTNAGG 1 cut(s) 987
BseBI CCWGG 2 cut(s) 286, 618
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 112, 285, 307
BseGI GGATG 2 cut(s) 103, 760
BseLI CCNNNNNNNGG 1 cut(s) 113
BseMII CTCAG 2 cut(s) 779, 978
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 5 cut(s) 238, 613, 713, 814, 943
BseYI CCCAGC 1 cut(s) 97
BshTI ACCGGT 2 cut(s) 148, 356
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 6 cut(s) 149, 184, 357, 399, 407, 738
BslI CCNNNNNNNGG 1 cut(s) 113
BsmAI GTCTC 3 cut(s) 391, 728, 876
BsmBI CGTCTC 1 cut(s) 391
Bso31I GGTCTC 1 cut(s) 728
BsoBI CYCGRG 1 cut(s) 25
Bsp119I TTCGAA 1 cut(s) 966
Bsp1286I GDGCHC 2 cut(s) 32, 554
Bsp1407I TGTACA 1 cut(s) 448
Bsp143I GATC 3 cut(s) 234, 342, 810
BspACI CCGC 4 cut(s) 5, 67, 88, 1067
BspCNI CTCAG 2 cut(s) 778, 979
BspDI ATCGAT 1 cut(s) 345
BspHI TCATGA 1 cut(s) 744
BspPI GGATC 2 cut(s) 350, 818
BspT104I TTCGAA 1 cut(s) 966
BspTNI GGTCTC 1 cut(s) 728
BsrFI RCCGGY 3 cut(s) 148, 356, 737
BsrGI TGTACA 1 cut(s) 448
BssAI RCCGGY 3 cut(s) 148, 356, 737
BssECI CCNNGG 3 cut(s) 112, 285, 307
BssMI GATC 3 cut(s) 234, 342, 810
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 286, 618
Bst4CI ACNGT 3 cut(s) 594, 823, 1045
Bst6I CTCTTC 2 cut(s) 540, 926
BstACI GRCGYC 1 cut(s) 381
BstAPI GCANNNNNTGC 2 cut(s) 1034, 1058
BstAUI TGTACA 1 cut(s) 448
BstBI TTCGAA 1 cut(s) 966
BstC8I GCNNGC 2 cut(s) 131, 1084
BstDEI CTNAG 3 cut(s) 470, 765, 987
BstF5I GGATG 2 cut(s) 103, 760
BstKTI GATC 3 cut(s) 237, 345, 813
BstMAI GTCTC 3 cut(s) 391, 728, 876
BstMBI GATC 3 cut(s) 234, 342, 810
BstMWI GCNNNNNNNGC 3 cut(s) 833, 1034, 1058
BstNI CCWGG 2 cut(s) 286, 618
BstSCI CCNGG 3 cut(s) 183, 284, 616
BstV1I GCAGC 5 cut(s) 238, 613, 713, 814, 943
BstV2I GAAGAC 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 866
Bsu15I ATCGAT 1 cut(s) 345
Bsu36I CCTNAGG 1 cut(s) 987
BsuI GTATCC 2 cut(s) 104, 664
BsuTUI ATCGAT 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 103, 760
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 1 cut(s) 1032
Cac8I GCNNGC 2 cut(s) 131, 1084
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 744
Cfr10I RCCGGY 3 cut(s) 148, 356, 737
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 449
CspAI ACCGGT 2 cut(s) 148, 356
CviAII CATG 2 cut(s) 626, 745
CviQI GTAC 1 cut(s) 449
DdeI CTNAG 3 cut(s) 470, 765, 987
DpnI GATC 3 cut(s) 236, 344, 812
DpnII GATC 3 cut(s) 234, 342, 810
Eam1104I CTCTTC 2 cut(s) 540, 926
EarI CTCTTC 2 cut(s) 540, 926
EciI GGCGGA 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 728
Eco57I CTGAAG 1 cut(s) 590
Eco81I CCTNAGG 1 cut(s) 987
Eco88I CYCGRG 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 284, 616
EcoT14I CCWWGG 1 cut(s) 307
EcoT22I ATGCAT 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 307
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 2 cut(s) 629, 748
FatI CATG 2 cut(s) 625, 744
FauI CCCGC 1 cut(s) 1074
Fnu4HI GCNGC 6 cut(s) 67, 252, 602, 702, 828, 957
FokI GGATG 2 cut(s) 90, 747
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 6 cut(s) 67, 252, 602, 702, 828, 957
FspBI CTAG 1 cut(s) 465
GluI GCNGC 6 cut(s) 67, 252, 602, 702, 828, 957
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 2 cut(s) 639, 713
HapII CCGG 6 cut(s) 149, 184, 357, 399, 407, 738
HgaI GACGC 1 cut(s) 389
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 2 cut(s) 629, 748
HincII GTYRAC 2 cut(s) 709, 1041
HindII GTYRAC 2 cut(s) 709, 1041
HindIII AAGCTT 2 cut(s) 275, 1084
HinfI GANTC 6 cut(s) 209, 245, 528, 621, 750, 946
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 6 cut(s) 149, 184, 357, 399, 407, 738
HphI GGTGA 6 cut(s) 70, 138, 251, 396, 509, 1055
Hpy166II GTNNAC 4 cut(s) 500, 562, 709, 1041
Hpy188I TCNGA 7 cut(s) 116, 159, 208, 214, 365, 768, 815
Hpy188III TCNNGA 5 cut(s) 25, 692, 745, 808, 928
Hpy8I GTNNAC 4 cut(s) 500, 562, 709, 1041
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 7 cut(s) 29, 45, 66, 667, 684, 691, 962
HpyCH4III ACNGT 3 cut(s) 594, 823, 1045
HpyCH4V TGCA 7 cut(s) 443, 724, 830, 854, 1028, 1075, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 833, 1034, 1058
HpyF3I CTNAG 3 cut(s) 470, 765, 987
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 2 cut(s) 629, 748
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 3 cut(s) 234, 342, 810
Lsp1109I GCAGC 5 cut(s) 238, 613, 713, 814, 943
LweI GCATC 5 cut(s) 711, 733, 750, 839, 933
MaeI CTAG 1 cut(s) 465
MaeIII GTNAC 2 cut(s) 144, 588
MalI GATC 3 cut(s) 236, 344, 812
MboI GATC 3 cut(s) 234, 342, 810
MfeI CAATTG 1 cut(s) 444
MhlI GDGCHC 2 cut(s) 32, 554
MluCI AATT 7 cut(s) 153, 162, 260, 349, 444, 646, 1004
MlyI GAGTC 2 cut(s) 203, 630
MmeI TCCRAC 1 cut(s) 754
Mph1103I ATGCAT 1 cut(s) 726
MseI TTAA 3 cut(s) 83, 422, 1040
MslI CAYNNNNRTG 2 cut(s) 579, 595
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 6 cut(s) 149, 184, 357, 399, 407, 738
MspR9I CCNGG 3 cut(s) 185, 286, 618
MunI CAATTG 1 cut(s) 444
MvaI CCWGG 2 cut(s) 286, 618
MwoI GCNNNNNNNGC 3 cut(s) 833, 1034, 1058
NciI CCSGG 1 cut(s) 185
NdeII GATC 3 cut(s) 234, 342, 810
NlaIII CATG 2 cut(s) 629, 748
NmuCI GTSAC 2 cut(s) 144, 588
NsiI ATGCAT 1 cut(s) 726
NspV TTCGAA 1 cut(s) 966
PaeR7I CTCGAG 1 cut(s) 25
PagI TCATGA 1 cut(s) 744
PcsI WCGNNNNNNNCGW 1 cut(s) 508
PfeI GAWTC 4 cut(s) 245, 528, 750, 946
PflFI GACNNNGTC 1 cut(s) 383
PfoI TCCNGGA 2 cut(s) 183, 616
PinAI ACCGGT 2 cut(s) 148, 356
PkrI GCNGC 6 cut(s) 68, 253, 603, 703, 829, 958
PleI GAGTC 2 cut(s) 203, 629
PpsI GAGTC 2 cut(s) 203, 629
PsiI TTATAA 1 cut(s) 569
Psp6I CCWGG 2 cut(s) 284, 616
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 2 cut(s) 284, 616
PstNI CAGNNNCTG 1 cut(s) 827
PsyI GACNNNGTC 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 1 cut(s) 450
RsaNI GTAC 1 cut(s) 449
RseI CAYNNNNRTG 2 cut(s) 579, 595
SaqAI TTAA 3 cut(s) 83, 422, 1040
SatI GCNGC 6 cut(s) 67, 252, 602, 702, 828, 957
Sau3AI GATC 3 cut(s) 234, 342, 810
SchI GAGTC 2 cut(s) 203, 630
ScrFI CCNGG 3 cut(s) 185, 286, 618
SduI GDGCHC 2 cut(s) 32, 554
SfaNI GCATC 5 cut(s) 711, 733, 750, 839, 933
Sfr274I CTCGAG 1 cut(s) 25
SfuI TTCGAA 1 cut(s) 966
SlaI CTCGAG 1 cut(s) 25
SmiMI CAYNNNNRTG 2 cut(s) 579, 595
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 7 cut(s) 153, 162, 260, 349, 444, 646, 1004
SsiI CCGC 4 cut(s) 5, 67, 88, 1067
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 3 cut(s) 183, 284, 616
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 3 cut(s) 594, 823, 1045
TaqI TCGA 6 cut(s) 26, 122, 345, 378, 757, 966
TasI AATT 7 cut(s) 153, 162, 260, 349, 444, 646, 1004
TatI WGTACW 1 cut(s) 448
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 4 cut(s) 245, 528, 750, 946
Tru1I TTAA 3 cut(s) 83, 422, 1040
Tru9I TTAA 3 cut(s) 83, 422, 1040
TscAI CASTG 1 cut(s) 1039
TseFI GTSAC 2 cut(s) 144, 588
TseI GCWGC 5 cut(s) 251, 601, 701, 827, 956
Tsp45I GTSAC 2 cut(s) 144, 588
TspDTI ATGAA 3 cut(s) 364, 642, 1085
TspRI CASTG 1 cut(s) 1039
Tth111I GACNNNGTC 1 cut(s) 383
XapI RAATTY 2 cut(s) 162, 1004
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XhoI CTCGAG 1 cut(s) 25
XspI CTAG 1 cut(s) 465
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.