Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010250

Physical Location & Seq

Genomic Coordinates Forward (+)

3203610 .. 3206753

Gene Structure 3144 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1028100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGGCCTCCTCCTCCTCCACGTTTGTCCTCTCAACCATCTTCTCTTCCTGTCCTCCCCAATCCCTAAACCCAGACAAGCCCAAGACCCCCTCCCTCTCATCCACCCGCCAAACCCTCAAAGCTTCCACCTTTCTGAAATCCAACCAACAACCCACTTTCCACCCCATCCCTCTCAACCAAATTCACTTGTCCCGCAAATTTCGCACCTTCATCACCGTCAGAGACGGCAGCGACGGCGACGGCGACGAGCAGCCGGTGGTGGGGGAGGACTCGGCTGAGTTTGTGTTGTCGAAGCAGAAGCTGTCCTCGTGGATTTATTTCACTGCAATTCTGGGTACTGTTCTGTTCATACTCAATGTAGCTTGGCTGGATAATTCCACCGGACTCGGAAAGCCCTTCGTCGACGCGGTTTCGGAACTTTCCGATAGCCCTGAGGTTGTGATGTTGATCCTGATTCTAATATTTGCCATTTTCCATAGTGGTTTGGCTAGTCTACGAGACACAGGTGAGAAACTTATTGGTGAACGTGCTTTCCGTGTTTTGTTTGCGGGGACATCTCTACCGTTGGCTGTTACAACTGTTGTGTTCTTTATTAACCACAGATACGATGGACTACAGTTATGGGATGTGAAGAATGTATTTGGTGTCCATGAACTTGTGTGGCTCTCAAATTTCGTTTCGTTCTTATTCCTTTATCCCTCAACTTTCAATCTACTAGAGGTAGCAGCCGTTGACAAGCCCAAAATGCATCTTTGGGAAACTGGGATTATGAGAATAACCAGGCATCCACAGCTGGCCGGACAGGTAATATGGTGCCTGGCTCACACGATTTGGATAGGGAACTCGGTGGCGGTGGCAGCTTCTCTTGGTTTAATCGGACACCACCTGTTTGGTGCTTGGAATGGTGACAGGAGATTAGCTATAAGATACGGGGAGGCCTTTGAAGTTGTGAAGAAACGAACAAGTATCATACCATTTGCAGCGATTCTTGATGGCCGACAAAAGTTGCCCAAAGATTACTACAAGGAATTTATTCGGTTGCCATATTTGTCAATCACTGCGTTGACGTTAGGTGCATACTTTGCACACCCCCTTATGCAGGCAGCCAGTTTTCGGCTCCATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016119 GO:0016120 GO:0016853 GO:0016859 GO:0042214 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046246 GO:0071704 GO:0090471 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

NnrU

Protein Analysis

376

Amino Acids

41.89

Weight (kDa)

9.11

Isoelectric Point (pI)

33.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NnrU	PF07298	147 - 366	5.2e-36	NnrU protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10830 AT1G10830 AT1G10830
fragaria_vesca	FvH4_4g12260 FvH4_4g12260 FvH4_4g12260
malus_domestica	MD04G1028100.v1.1
prunus_persica	Prupe.1G177500_v2.0.a1
pyrus_communis	pycom04g02470
rosa_chinensis	RchiOBHm_Chr4g0410061
rosa_laevigata	RLG00000008505
rosa_multiflora	Rmu_co8413299.1_g000001 Rmu_sc0000365.1_g000059 Rmu_sc0002691.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354590
rosa_rugosa	Rorug04G0100800
rosa_samantha	Rh4AG159100 Rh4BG158400 Rh4CG170400 Rh4DG154200
rosa_wichuraiana	Rw4G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	813
AccI	GTMKAC	2 cut(s)	402, 493
AccII	CGCG	1 cut(s)	407
AciI	CCGC	5 cut(s)	106, 193, 407, 548, 851
AclWI	GGATC	1 cut(s)	442
AcoI	YGGCCR	2 cut(s)	795, 994
AcsI	RAATTY	4 cut(s)	180, 197, 670, 1028
AfaI	GTAC	1 cut(s)	337
AfiI	CCNNNNNNNGG	3 cut(s)	260, 1099, 1113
AgsI	TTSAA	2 cut(s)	709, 944
AjnI	CCWGG	2 cut(s)	779, 816
AloI	GAACNNNNNNTCC	2 cut(s)	516, 548
AluBI	AGCT	6 cut(s)	122, 301, 362, 793, 860, 920
AluI	AGCT	6 cut(s)	122, 301, 362, 793, 860, 920
Alw26I	GTCTC	2 cut(s)	216, 492
AlwI	GGATC	1 cut(s)	442
AlwNI	CAGNNNCTG	1 cut(s)	301
AoxI	GGCC	4 cut(s)	3, 795, 936, 994
ApeKI	GCWGC	6 cut(s)	228, 250, 725, 857, 980, 1103
ApoI	RAATTY	4 cut(s)	180, 197, 670, 1028
Asp700I	GAANNNNTTC	1 cut(s)	1032
AsuHPI	GGTGA	4 cut(s)	205, 518, 533, 917
AxyI	CCTNAGG	1 cut(s)	432
BanI	GGYRCC	1 cut(s)	813
BauI	CACGAG	1 cut(s)	307
BbvI	GCAGC	6 cut(s)	240, 262, 737, 869, 992, 1115
BccI	CCATC	4 cut(s)	44, 173, 602, 986
BceAI	ACGGC	4 cut(s)	241, 250, 256, 713
BciT130I	CCWGG	2 cut(s)	781, 818
BcoDI	GTCTC	2 cut(s)	216, 492
BfaI	CTAG	2 cut(s)	489, 716
BfmI	CTRYAG	1 cut(s)	614
BisI	GCNGC	6 cut(s)	229, 251, 726, 858, 981, 1104
BlsI	GCNGC	6 cut(s)	230, 252, 727, 859, 982, 1105
Bme1390I	CCNGG	2 cut(s)	781, 818
BmiI	GGNNCC	2 cut(s)	815, 1118
BmrFI	CCNGG	2 cut(s)	781, 818
BmrI	ACTGGG	1 cut(s)	771
BmsI	GCATC	2 cut(s)	757, 793
BmuI	ACTGGG	1 cut(s)	771
BsaBI	GATNNNNATC	1 cut(s)	446
BsaWI	WCCGGW	1 cut(s)	380
Bsc4I	CCNNNNNNNGG	3 cut(s)	260, 1099, 1113
Bse118I	RCCGGY	1 cut(s)	253
Bse1I	ACTGG	2 cut(s)	766, 1107
Bse21I	CCTNAGG	1 cut(s)	432
Bse8I	GATNNNNATC	1 cut(s)	446
BseBI	CCWGG	2 cut(s)	781, 818
BseGI	GGATG	4 cut(s)	98, 165, 631, 784
BseJI	GATNNNNATC	1 cut(s)	446
BseLI	CCNNNNNNNGG	3 cut(s)	260, 1099, 1113
BseMII	CTCAG	2 cut(s)	267, 423
BseNI	ACTGG	2 cut(s)	766, 1107
BseRI	GAGGAG	1 cut(s)	4
BseXI	GCAGC	6 cut(s)	240, 262, 737, 869, 992, 1115
Bsh1236I	CGCG	1 cut(s)	407
BshFI	GGCC	4 cut(s)	5, 797, 938, 996
BshNI	GGYRCC	1 cut(s)	813
BsiSI	CCGG	3 cut(s)	254, 381, 798
BslFI	GGGAC	2 cut(s)	175, 565
BslI	CCNNNNNNNGG	3 cut(s)	260, 1099, 1113
BsmAI	GTCTC	2 cut(s)	216, 492
BsmBI	CGTCTC	1 cut(s)	216
BsmFI	GGGAC	2 cut(s)	175, 565
BsnI	GGCC	4 cut(s)	5, 797, 938, 996
Bsp143I	GATC	1 cut(s)	447
BspACI	CCGC	5 cut(s)	106, 193, 407, 548, 851
BspANI	GGCC	4 cut(s)	5, 797, 938, 996
BspCNI	CTCAG	2 cut(s)	268, 424
BspFNI	CGCG	1 cut(s)	407
BspLI	GGNNCC	2 cut(s)	815, 1118
BspPI	GGATC	1 cut(s)	442
BspT107I	GGYRCC	1 cut(s)	813
BsrFI	RCCGGY	1 cut(s)	253
BsrI	ACTGG	2 cut(s)	766, 1107
BssAI	RCCGGY	1 cut(s)	253
BssMI	GATC	1 cut(s)	447
BssSI	CACGAG	1 cut(s)	307
Bst2BI	CACGAG	1 cut(s)	307
Bst2UI	CCWGG	2 cut(s)	781, 818
Bst4CI	ACNGT	5 cut(s)	217, 340, 564, 580, 618
Bst6I	CTCTTC	1 cut(s)	49
BstAPI	GCANNNNNTGC	1 cut(s)	1082
BstC8I	GCNNGC	2 cut(s)	795, 1101
BstDEI	CTNAG	2 cut(s)	276, 432
BstENI	CCTNNNNNAGG	1 cut(s)	1097
BstF5I	GGATG	4 cut(s)	98, 165, 631, 784
BstFNI	CGCG	1 cut(s)	407
BstKTI	GATC	1 cut(s)	450
BstMAI	GTCTC	2 cut(s)	216, 492
BstMBI	GATC	1 cut(s)	447
BstMWI	GCNNNNNNNGC	6 cut(s)	201, 234, 745, 790, 857, 1082
BstNI	CCWGG	2 cut(s)	781, 818
BstSCI	CCNGG	2 cut(s)	779, 816
BstSFI	CTRYAG	1 cut(s)	614
BstUI	CGCG	1 cut(s)	407
BstV1I	GCAGC	6 cut(s)	240, 262, 737, 869, 992, 1115
BstXI	CCANNNNNNTGG	1 cut(s)	890
Bsu36I	CCTNAGG	1 cut(s)	432
BsuRI	GGCC	4 cut(s)	5, 797, 938, 996
BtsCI	GGATG	4 cut(s)	98, 165, 631, 784
BtsI	GCAGTG	2 cut(s)	321, 1056
BtsIMutI	CAGTG	2 cut(s)	321, 1056
Cac8I	GCNNGC	2 cut(s)	795, 1101
CaiI	CAGNNNCTG	1 cut(s)	301
Cfr10I	RCCGGY	1 cut(s)	253
CseI	GACGC	1 cut(s)	413
Csp6I	GTAC	1 cut(s)	336
CviAII	CATG	1 cut(s)	650
CviQI	GTAC	1 cut(s)	336
DdeI	CTNAG	2 cut(s)	276, 432
DpnI	GATC	1 cut(s)	449
DpnII	GATC	1 cut(s)	447
EaeI	YGGCCR	2 cut(s)	795, 994
Eam1104I	CTCTTC	1 cut(s)	49
EarI	CTCTTC	1 cut(s)	49
Eco147I	AGGCCT	1 cut(s)	938
Eco81I	CCTNAGG	1 cut(s)	432
EcoNI	CCTNNNNNAGG	1 cut(s)	1097
EcoRII	CCWGG	2 cut(s)	779, 816
EcoT22I	ATGCAT	1 cut(s)	750
Esp3I	CGTCTC	1 cut(s)	216
FaeI	CATG	1 cut(s)	653
FaqI	GGGAC	2 cut(s)	175, 565
FatI	CATG	1 cut(s)	649
FauI	CCCGC	3 cut(s)	113, 200, 541
FblI	GTMKAC	2 cut(s)	402, 493
Fnu4HI	GCNGC	6 cut(s)	229, 251, 726, 858, 981, 1104
FokI	GGATG	4 cut(s)	85, 152, 638, 771
Fsp4HI	GCNGC	6 cut(s)	229, 251, 726, 858, 981, 1104
FspBI	CTAG	2 cut(s)	489, 716
GluI	GCNGC	6 cut(s)	229, 251, 726, 858, 981, 1104
HaeIII	GGCC	4 cut(s)	5, 797, 938, 996
HapII	CCGG	3 cut(s)	254, 381, 798
HgaI	GACGC	1 cut(s)	413
Hin1II	CATG	1 cut(s)	653
HincII	GTYRAC	3 cut(s)	403, 733, 1065
HindII	GTYRAC	3 cut(s)	403, 733, 1065
HindIII	AAGCTT	1 cut(s)	120
HinfI	GANTC	4 cut(s)	269, 384, 454, 985
HpaII	CCGG	3 cut(s)	254, 381, 798
HphI	GGTGA	4 cut(s)	205, 518, 533, 917
Hpy166II	GTNNAC	5 cut(s)	403, 494, 524, 733, 1065
Hpy188I	TCNGA	6 cut(s)	135, 221, 389, 415, 424, 878
Hpy188III	TCNNGA	2 cut(s)	451, 989
Hpy8I	GTNNAC	5 cut(s)	403, 494, 524, 733, 1065
Hpy99I	CGWCG	5 cut(s)	236, 242, 248, 404, 407
HpyAV	CCTTC	2 cut(s)	217, 406
HpyCH4III	ACNGT	5 cut(s)	217, 340, 564, 580, 618
HpyCH4IV	ACGT	3 cut(s)	20, 526, 1067
HpyCH4V	TGCA	6 cut(s)	326, 748, 980, 1076, 1085, 1099
HpyF10VI	GCNNNNNNNGC	6 cut(s)	201, 234, 745, 790, 857, 1082
HpyF3I	CTNAG	2 cut(s)	276, 432
HpySE526I	ACGT	3 cut(s)	20, 526, 1067
Hsp92II	CATG	1 cut(s)	653
Kzo9I	GATC	1 cut(s)	447
LmnI	GCTCC	1 cut(s)	1122
Lsp1109I	GCAGC	6 cut(s)	240, 262, 737, 869, 992, 1115
LweI	GCATC	2 cut(s)	757, 793
MaeI	CTAG	2 cut(s)	489, 716
MaeII	ACGT	3 cut(s)	20, 526, 1067
MaeIII	GTNAC	2 cut(s)	571, 905
MalI	GATC	1 cut(s)	449
MboI	GATC	1 cut(s)	447
MboII	GAAGA	4 cut(s)	31, 36, 643, 964
MluCI	AATT	6 cut(s)	180, 197, 327, 373, 670, 1028
MlyI	GAGTC	2 cut(s)	263, 378
MmeI	TCCRAC	1 cut(s)	165
Mph1103I	ATGCAT	1 cut(s)	750
MroXI	GAANNNNTTC	1 cut(s)	1032
MseI	TTAA	2 cut(s)	594, 872
MspA1I	CMGCKG	1 cut(s)	793
MspI	CCGG	3 cut(s)	254, 381, 798
MspR9I	CCNGG	2 cut(s)	781, 818
MvaI	CCWGG	2 cut(s)	781, 818
MvnI	CGCG	1 cut(s)	407
MwoI	GCNNNNNNNGC	6 cut(s)	201, 234, 745, 790, 857, 1082
NdeII	GATC	1 cut(s)	447
NlaIII	CATG	1 cut(s)	653
NlaIV	GGNNCC	2 cut(s)	815, 1118
NmeAIII	GCCGAG	1 cut(s)	251
NmuCI	GTSAC	1 cut(s)	905
NsiI	ATGCAT	1 cut(s)	750
PceI	AGGCCT	1 cut(s)	938
PcsI	WCGNNNNNNNCGW	2 cut(s)	240, 532
PdmI	GAANNNNTTC	1 cut(s)	1032
PfeI	GAWTC	2 cut(s)	454, 985
PkrI	GCNGC	6 cut(s)	230, 252, 727, 859, 982, 1105
PleI	GAGTC	2 cut(s)	263, 378
PpsI	GAGTC	2 cut(s)	263, 378
Psp6I	CCWGG	2 cut(s)	779, 816
PspGI	CCWGG	2 cut(s)	779, 816
PspN4I	GGNNCC	2 cut(s)	815, 1118
PstNI	CAGNNNCTG	1 cut(s)	301
PvuII	CAGCTG	1 cut(s)	793
RsaI	GTAC	1 cut(s)	337
RsaNI	GTAC	1 cut(s)	336
SalI	GTCGAC	1 cut(s)	401
SaqAI	TTAA	2 cut(s)	594, 872
SatI	GCNGC	6 cut(s)	229, 251, 726, 858, 981, 1104
Sau3AI	GATC	1 cut(s)	447
SchI	GAGTC	2 cut(s)	263, 378
ScrFI	CCNGG	2 cut(s)	781, 818
SfaNI	GCATC	2 cut(s)	757, 793
SfcI	CTRYAG	1 cut(s)	614
SgrDI	CGTCGACG	1 cut(s)	401
Sse9I	AATT	6 cut(s)	180, 197, 327, 373, 670, 1028
SseBI	AGGCCT	1 cut(s)	938
SsiI	CCGC	5 cut(s)	106, 193, 407, 548, 851
SspI	AATATT	1 cut(s)	462
SspMI	CTAG	2 cut(s)	489, 716
StuI	AGGCCT	1 cut(s)	938
StyD4I	CCNGG	2 cut(s)	779, 816
TaaI	ACNGT	5 cut(s)	217, 340, 564, 580, 618
TaiI	ACGT	3 cut(s)	23, 529, 1070
TaqI	TCGA	2 cut(s)	290, 402
TasI	AATT	6 cut(s)	180, 197, 327, 373, 670, 1028
TfiI	GAWTC	2 cut(s)	454, 985
Tru1I	TTAA	2 cut(s)	594, 872
Tru9I	TTAA	2 cut(s)	594, 872
TscAI	CASTG	2 cut(s)	328, 1063
TseFI	GTSAC	1 cut(s)	905
TseI	GCWGC	6 cut(s)	228, 250, 725, 857, 980, 1103
Tsp45I	GTSAC	1 cut(s)	905
TspDTI	ATGAA	3 cut(s)	199, 337, 666
TspGWI	ACGGA	1 cut(s)	524
TspRI	CASTG	2 cut(s)	328, 1063
XagI	CCTNNNNNAGG	1 cut(s)	1097
XapI	RAATTY	4 cut(s)	180, 197, 670, 1028
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XmiI	GTMKAC	2 cut(s)	402, 493
XmnI	GAANNNNTTC	1 cut(s)	1032
XspI	CTAG	2 cut(s)	489, 716
Zsp2I	ATGCAT	1 cut(s)	750

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.