Rh4DG154200

15-cis-zeta-carotene isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010250
Physical Location & Seq
Reverse (-)
30729795 .. 30732841
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG154200.1

Sequence Viewer

Length: 1128 bp
ATGGCTTCCTCCTCCACCCTTGTCCTCTCCACCCTCTTCTCTTCTTTCCCTCTCCAAAAAACCCTAAACCCAAAAACCAAGAACCCCTTCTCTCTCACTTCCCACAAACTCCCTAGGCCTCCTTCTTCCCTGACCCTCAGCTGTTCACCGTTTCTGAAACCCAAACCCCATCACAATCAGATACAACTGTGCAGGAGATTTCGAGCCCGCACCACCATCAGAGATGTCGATAAGGACGACGAGGAGGAGCAGGCAGTTGTGGGAGAAGACTCGGCTGAGTTTCTCTTGTCAAAGCAGAAGCTGTCTTCCTGGCTTTATTTCACTGCAATTCTCGGGACTGTTCTGTTTGTGCTCAACGTGGCTTGGATTGATAATTCCACCGGGTTTGGGAAGCCTTTCATCGAAGCGGTTTCGGGGCTTTCTGATAGCCATGAGGTTGTAATGTTGCTCCTGATTCTTATATTTGCCATAGTCCATAGTGGTTTGGCTAGCCTACGAGACGCTGGTGAGAAGCTTATTGGAGAACGTGCTTTCCGCGTTTTGTTTGCAGGGACATCTTTACCATTGGCTGTTAGTACTGTTGTATATTTCATTAACCACAGATATGATGGAGTACAGTTATGGCAGCTCAAGGATGTTTATGGTGTACATGAATTTGTGTGGTTGTCTAATTTTATTTCCTTCCTCTTCCTTTATCCATCGACTTTCAATCTACTAGAGGTAGCAGCTGTTGACAAGCCCAAAATGCATCTTTGGGAAACTGGGATTATGAGAATCACGAGGCATCCACAGCTTGCAGGGCAGGTGGTTTGGTGCTTGGCTCACACAATTTGGATCGGAAACTCGGTAACAGTGGCAGCTTCTATTGGTTTAATTGGGCACCACCTATTTGGTGCTTGGAATGGTGACAGGAGATTAGCTATAAGATATGGGGAAAACTTTGAAGTTGTGAAGAAGCGAACAAGTATTATACCTTTTGCAGCCATTATTGATGGCCGTCAAAAGTTGCCAAAAGATTACTACAAGGAATTTATTCGGTTGCCTTATTTTGTGATCACTGCATTGACGTTAGGTGCATACTTTGCACACCCCCTTATGCAGGCAGCCAGTTTTGGGCTCCATTGGTAG

Protein Analysis

375

Amino Acids

42.24

Weight (kDa)

9.31

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NnrU PF07298 147 - 366 4.1e-36 NnrU protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 793
Acc36I ACCTGC 1 cut(s) 793
AccB1I GGYRCC 1 cut(s) 879
AccII CGCG 1 cut(s) 537
AciI CCGC 3 cut(s) 208, 407, 535
AclWI GGATC 1 cut(s) 842
AcoI YGGCCR 1 cut(s) 994
AcsI RAATTY 2 cut(s) 653, 1028
AfaI GTAC 3 cut(s) 577, 615, 648
AfiI CCNNNNNNNGG 3 cut(s) 387, 1099, 1113
AgsI TTSAA 2 cut(s) 709, 944
AjnI CCWGG 1 cut(s) 308
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AloI GAACNNNNNNTCC 2 cut(s) 516, 548
AluBI AGCT 8 cut(s) 141, 301, 514, 628, 728, 793, 860, 920
AluI AGCT 8 cut(s) 141, 301, 514, 628, 728, 793, 860, 920
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 1 cut(s) 842
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 2 cut(s) 116, 994
ApeKI GCWGC 5 cut(s) 625, 725, 857, 980, 1103
ApoI RAATTY 2 cut(s) 653, 1028
Asp700I GAANNNNTTC 3 cut(s) 86, 395, 1032
AspA2I CCTAGG 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 3 cut(s) 138, 518, 917
AsuNHI GCTAGC 1 cut(s) 488
AvaI CYCGRG 1 cut(s) 332
AvrII CCTAGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 882
BaeI ACNNNNGTAYC 2 cut(s) 173, 206
BanI GGYRCC 1 cut(s) 879
BanII GRGCYC 2 cut(s) 208, 1119
BauI CACGAG 1 cut(s) 778
BbsI GAAGAC 2 cut(s) 273, 297
Bbv12I GWGCWC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 5 cut(s) 637, 737, 869, 992, 1115
BccI CCATC 5 cut(s) 177, 224, 602, 706, 986
BceAI ACGGC 1 cut(s) 981
BciT130I CCWGG 1 cut(s) 310
BclI TGATCA 1 cut(s) 1053
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 3 cut(s) 114, 489, 716
BfuAI ACCTGC 1 cut(s) 793
BisI GCNGC 5 cut(s) 626, 726, 858, 981, 1104
BlnI CCTAGG 1 cut(s) 113
BlsI GCNGC 5 cut(s) 627, 727, 859, 982, 1105
BmcAI AGTACT 1 cut(s) 577
Bme1390I CCNGG 2 cut(s) 310, 382
BmeT110I CYCGRG 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 881, 1118
BmrFI CCNGG 2 cut(s) 310, 382
BmrI ACTGGG 1 cut(s) 771
BmsI GCATC 2 cut(s) 757, 793
BmtI GCTAGC 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 771
BpiI GAAGAC 2 cut(s) 273, 297
Bpu10I CCTNAGC 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 614
BpuMI CCSGG 1 cut(s) 382
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 1099, 1113
Bse1I ACTGG 2 cut(s) 766, 1107
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 2 cut(s) 640, 784
BseLI CCNNNNNNNGG 3 cut(s) 387, 1099, 1113
BseMII CTCAG 2 cut(s) 151, 267
BseNI ACTGG 2 cut(s) 766, 1107
BseRI GAGGAG 2 cut(s) 257, 260
BseSI GKGCMC 1 cut(s) 882
BseXI GCAGC 5 cut(s) 637, 737, 869, 992, 1115
BsgI GTGCAG 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 118, 996
BshNI GGYRCC 1 cut(s) 879
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 381
BslFI GGGAC 2 cut(s) 349, 565
BslI CCNNNNNNNGG 3 cut(s) 387, 1099, 1113
BsmAI GTCTC 1 cut(s) 492
BsmBI CGTCTC 1 cut(s) 492
BsmFI GGGAC 2 cut(s) 349, 565
BsnI GGCC 2 cut(s) 118, 996
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 4 cut(s) 208, 354, 882, 1119
Bsp1407I TGTACA 1 cut(s) 646
Bsp143I GATC 2 cut(s) 834, 1053
BspACI CCGC 3 cut(s) 208, 407, 535
BspANI GGCC 2 cut(s) 118, 996
BspCNI CTCAG 2 cut(s) 150, 268
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 881, 1118
BspMI ACCTGC 1 cut(s) 793
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 1 cut(s) 842
BspT107I GGYRCC 1 cut(s) 879
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 2 cut(s) 766, 1107
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 2 cut(s) 834, 1053
BssSI CACGAG 1 cut(s) 778
BssT1I CCWWGG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 778
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 6 cut(s) 150, 189, 340, 580, 618, 853
Bst6I CTCTTC 3 cut(s) 41, 46, 692
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstAUI TGTACA 1 cut(s) 646
BstC8I GCNNGC 5 cut(s) 208, 252, 490, 795, 1101
BstDEI CTNAG 2 cut(s) 137, 276
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 2 cut(s) 640, 784
BstFNI CGCG 1 cut(s) 537
BstKTI GATC 2 cut(s) 837, 1056
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 2 cut(s) 834, 1053
BstMWI GCNNNNNNNGC 4 cut(s) 745, 790, 799, 1082
BstNI CCWGG 1 cut(s) 310
BstSCI CCNGG 2 cut(s) 308, 380
BstSLI GKGCMC 1 cut(s) 882
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 5 cut(s) 637, 737, 869, 992, 1115
BstV2I GAAGAC 2 cut(s) 273, 297
BstXI CCANNNNNNTGG 1 cut(s) 890
BsuRI GGCC 2 cut(s) 118, 996
BtsCI GGATG 2 cut(s) 640, 784
BtsI GCAGTG 2 cut(s) 321, 1056
BtsIMutI CAGTG 3 cut(s) 321, 858, 1056
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 5 cut(s) 208, 252, 490, 795, 1101
CaiI CAGNNNCTG 1 cut(s) 301
CseI GACGC 1 cut(s) 509
Csp6I GTAC 3 cut(s) 576, 614, 647
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 2 cut(s) 431, 650
CviQI GTAC 3 cut(s) 576, 614, 647
DdeI CTNAG 2 cut(s) 137, 276
DpnI GATC 2 cut(s) 836, 1055
DpnII GATC 2 cut(s) 834, 1053
EaeI YGGCCR 1 cut(s) 994
Eam1104I CTCTTC 3 cut(s) 41, 46, 692
EarI CTCTTC 3 cut(s) 41, 46, 692
Eco130I CCWWGG 1 cut(s) 113
Eco147I AGGCCT 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 208, 1119
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 750
EcoT38I GRGCYC 2 cut(s) 208, 1119
ErhI CCWWGG 1 cut(s) 113
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 2 cut(s) 434, 653
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 2 cut(s) 349, 565
FatI CATG 2 cut(s) 430, 649
FauI CCCGC 1 cut(s) 215
FbaI TGATCA 1 cut(s) 1053
Fnu4HI GCNGC 5 cut(s) 626, 726, 858, 981, 1104
FokI GGATG 2 cut(s) 647, 771
FriOI GRGCYC 2 cut(s) 208, 1119
Fsp4HI GCNGC 5 cut(s) 626, 726, 858, 981, 1104
FspBI CTAG 3 cut(s) 114, 489, 716
GluI GCNGC 5 cut(s) 626, 726, 858, 981, 1104
HaeIII GGCC 2 cut(s) 118, 996
HapII CCGG 1 cut(s) 381
HgaI GACGC 1 cut(s) 509
Hin1II CATG 2 cut(s) 434, 653
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 3 cut(s) 269, 454, 774
HpaII CCGG 1 cut(s) 381
HphI GGTGA 3 cut(s) 138, 518, 917
Hpy166II GTNNAC 3 cut(s) 146, 647, 733
Hpy188I TCNGA 5 cut(s) 156, 180, 221, 424, 839
Hpy188III TCNNGA 3 cut(s) 334, 451, 778
Hpy8I GTNNAC 3 cut(s) 146, 647, 733
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 97, 132, 691
HpyCH4III ACNGT 6 cut(s) 150, 189, 340, 580, 618, 853
HpyCH4IV ACGT 3 cut(s) 357, 526, 1067
HpyF10VI GCNNNNNNNGC 4 cut(s) 745, 790, 799, 1082
HpyF3I CTNAG 2 cut(s) 137, 276
HpySE526I ACGT 3 cut(s) 357, 526, 1067
Hsp92II CATG 2 cut(s) 434, 653
Ksp22I TGATCA 1 cut(s) 1053
Kzo9I GATC 2 cut(s) 834, 1053
LmnI GCTCC 3 cut(s) 247, 453, 1122
Lsp1109I GCAGC 5 cut(s) 637, 737, 869, 992, 1115
LweI GCATC 2 cut(s) 757, 793
MaeI CTAG 3 cut(s) 114, 489, 716
MaeII ACGT 3 cut(s) 357, 526, 1067
MaeIII GTNAC 2 cut(s) 847, 905
MalI GATC 2 cut(s) 836, 1055
MboI GATC 2 cut(s) 834, 1053
MboII GAAGA 7 cut(s) 28, 33, 117, 278, 297, 679, 964
MhlI GDGCHC 4 cut(s) 208, 354, 882, 1119
MluCI AATT 7 cut(s) 327, 373, 653, 670, 828, 873, 1028
MlyI GAGTC 1 cut(s) 263
Mph1103I ATGCAT 1 cut(s) 750
MroXI GAANNNNTTC 3 cut(s) 86, 395, 1032
MseI TTAA 2 cut(s) 594, 872
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 2 cut(s) 141, 728
MspI CCGG 1 cut(s) 381
MspR9I CCNGG 2 cut(s) 310, 382
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 4 cut(s) 745, 790, 799, 1082
NciI CCSGG 1 cut(s) 382
NdeII GATC 2 cut(s) 834, 1053
NheI GCTAGC 1 cut(s) 488
NlaIII CATG 2 cut(s) 434, 653
NlaIV GGNNCC 2 cut(s) 881, 1118
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 905
NsiI ATGCAT 1 cut(s) 750
PaqCI CACCTGC 1 cut(s) 793
PceI AGGCCT 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdmI GAANNNNTTC 3 cut(s) 86, 395, 1032
PfeI GAWTC 2 cut(s) 454, 774
PkrI GCNGC 5 cut(s) 627, 727, 859, 982, 1105
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 308
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 2 cut(s) 881, 1118
PstNI CAGNNNCTG 1 cut(s) 301
PvuII CAGCTG 2 cut(s) 141, 728
RsaI GTAC 3 cut(s) 577, 615, 648
RsaNI GTAC 3 cut(s) 576, 614, 647
RseI CAYNNNNRTG 1 cut(s) 603
SaqAI TTAA 2 cut(s) 594, 872
SatI GCNGC 5 cut(s) 626, 726, 858, 981, 1104
Sau3AI GATC 2 cut(s) 834, 1053
ScaI AGTACT 1 cut(s) 577
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 2 cut(s) 310, 382
SduI GDGCHC 4 cut(s) 208, 354, 882, 1119
SfaNI GCATC 2 cut(s) 757, 793
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 7 cut(s) 327, 373, 653, 670, 828, 873, 1028
SseBI AGGCCT 1 cut(s) 118
SsiI CCGC 3 cut(s) 208, 407, 535
SspMI CTAG 3 cut(s) 114, 489, 716
StuI AGGCCT 1 cut(s) 118
StyD4I CCNGG 2 cut(s) 308, 380
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 6 cut(s) 150, 189, 340, 580, 618, 853
TaiI ACGT 3 cut(s) 360, 529, 1070
TaqI TCGA 4 cut(s) 202, 228, 402, 701
TasI AATT 7 cut(s) 327, 373, 653, 670, 828, 873, 1028
TatI WGTACW 3 cut(s) 575, 613, 646
TfiI GAWTC 2 cut(s) 454, 774
Tru1I TTAA 2 cut(s) 594, 872
Tru9I TTAA 2 cut(s) 594, 872
TscAI CASTG 3 cut(s) 328, 858, 1063
TseFI GTSAC 1 cut(s) 905
TseI GCWGC 5 cut(s) 625, 725, 857, 980, 1103
Tsp45I GTSAC 1 cut(s) 905
TspDTI ATGAA 3 cut(s) 388, 580, 666
TspRI CASTG 3 cut(s) 328, 858, 1063
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 2 cut(s) 653, 1028
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XmaJI CCTAGG 1 cut(s) 113
XmnI GAANNNNTTC 3 cut(s) 86, 395, 1032
XspI CTAG 3 cut(s) 114, 489, 716
ZrmI AGTACT 1 cut(s) 577
Zsp2I ATGCAT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.