AT1G12420

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012060
Physical Location & Seq
Reverse (-)
4226239 .. 4229211
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12420.1

Sequence Viewer

Length: 1326 bp
ATGGCGATGAAGGGTTATTTGGATGAGTATGAAAAGCTTGTTATTAGGATGAACACTCCAAGGGTCGTTATCGACAATGGTGTTTGTTCTTCAGCGACAATCGTCAAGGTTGATAGTTCAAGAAGAAATGGGATATTGTTAGAAGCTGTACAAATTCTCACAGATTTGAATCTCTCCATTAAAAAAGCTTACATTTCTTCTGATGGAACATGGAACATGGATGTTTTTCATGTCACTGACTTAAACGGAAACAAATTGAATGACCAGAGCGTCTTGAGATACATTGAACAGTCGATCGAGACGGTTTACTATGGAGAAAACATTGAAGTAAACGGTTTAACAGCTTTGGAGTTAACCGGAACAGACCGAATCGGTTTACTATCTGAGATGTTTGCGGTTCTCTCTGATCTCAACTGTGATGTGGTTGACGCTAAGCTATGGACACATAACGGTAGAGTTGCTTCTGTGATCTATCTCAAAGATTGTATCTCAGGAGCTCCGATTCTCGATTCTCACCGGATATCAAAGATCGAAGGACGGTTAAAGAACGTTTTGAATGGCGATAACGATGTTAACTCTGCTGCAAAGACTTGTGTTACTGTGGATTCGATGATGCATATCGAACGTAGACTTCATCAGCTTATGTTTGAAGATAGAGACTACGAGAGGAGATCTAAGAAACATGAGAGATCTCCAATGGTGGTTGTGACGGTTCAGAATTGGGCTGAGAGGGGTTACTCTGTCGTTAATGTTCATTGTCGGGACCGGACCAAGCTTCTATTCGATGTGGTTTGTACGTTAACCGATATGGAATATGCTGTGTTTCATGCTACTATCAACACAGCTGAAGACCAAGCTCACTTGGAATTCTATATCCGGCATAAGGATGGATCACCGATAAGTTCGGAAGCAGAGAGGCAACGAGTGATTCAATGCTTAGAGGCTGCAGTTGAAAGAAGAGCACTAGAGGGAGTGAGATTAGAGCTGAGGCATCCGGACAAACAAGGTTTACTAGCGGAAGTAACGCGGACATTCAGAGAAAACGGTCTGAATGTTACAAGAACAGAGATATCAACGAGTAGCGACATGGCTACAAACATATTCTATGTAACTGATGCAAATGGAGACGAACCGGACTTCAAATTGATTGAATCGGTTAGGGAGAAAATCGGTTTGGAGTGTTTGAGAGTGAAGGAGATGCCAACAATGTATCATAAGAAGGGAGATGGAGAAGAACAACAACAAACCAAAGCAGTGTTGGTTTCACTTGGAAGTTTGGTTTGGAGAAATCTATTCAACTTTGGTCTTATCAAATCATGTTCTTAA

Protein Analysis

441

Amino Acids

49.96

Weight (kDa)

5.72

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT_ACR9_3rd PF24931 236 - 318 8.8e-09 ACR9-like, ACT-like domain
ACT PF01842 248 - 307 3.8e-06 ACT domain
ACT PF01842 326 - 386 1.2e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 628
AccII CGCG 1 cut(s) 1027
AccIII TCCGGA 1 cut(s) 994
AciI CCGC 3 cut(s) 395, 1016, 1027
AclI AACGTT 1 cut(s) 549
AclWI GGATC 1 cut(s) 898
AcsI RAATTY 2 cut(s) 153, 866
AcuI CTGAAG 2 cut(s) 75, 867
AfaI GTAC 2 cut(s) 150, 796
AfiI CCNNNNNNNGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 7 cut(s) 34, 764, 796, 1157, 1189, 1264, 1296
Alw21I GWGCWC 2 cut(s) 499, 964
Alw26I GTCTC 3 cut(s) 293, 651, 1119
AlwI GGATC 1 cut(s) 898
Aor13HI TCCGGA 1 cut(s) 994
ApeKI GCWGC 2 cut(s) 581, 944
ApoI RAATTY 2 cut(s) 153, 866
AspS9I GGNCC 2 cut(s) 763, 768
AsuHPI GGTGA 2 cut(s) 506, 885
AvaII GGWCC 2 cut(s) 763, 768
BanII GRGCYC 1 cut(s) 499
BarI GAAGNNNNNNTAC 2 cut(s) 445, 477
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 499, 964
BbvCI CCTCAGC 1 cut(s) 986
BbvI GCAGC 2 cut(s) 568, 931
BccI CCATC 3 cut(s) 197, 881, 1220
BcoDI GTCTC 3 cut(s) 293, 651, 1119
BfaI CTAG 2 cut(s) 965, 1013
BfmI CTRYAG 1 cut(s) 945
BglII AGATCT 2 cut(s) 671, 689
BisI GCNGC 2 cut(s) 582, 945
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 2 cut(s) 583, 946
Bme18I GGWCC 2 cut(s) 763, 768
BmgT120I GGNCC 2 cut(s) 763, 768
BmiI GGNNCC 1 cut(s) 764
BmsI GCATC 4 cut(s) 603, 1000, 1105, 1188
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 855
Bpu10I CCTNAGC 1 cut(s) 986
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 295
BsaBI GATNNNNATC 2 cut(s) 168, 617
BsaJI CCNNGG 1 cut(s) 59
BsaWI WCCGGW 5 cut(s) 356, 516, 765, 994, 1132
Bsc4I CCNNNNNNNGG 1 cut(s) 883
Bse8I GATNNNNATC 2 cut(s) 168, 617
BseAI TCCGGA 1 cut(s) 994
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 5 cut(s) 28, 54, 226, 892, 991
BseJI GATNNNNATC 2 cut(s) 168, 617
BseLI CCNNNNNNNGG 1 cut(s) 883
BseMII CTCAG 4 cut(s) 375, 504, 717, 977
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 2 cut(s) 568, 931
Bsh1236I CGCG 1 cut(s) 1027
Bsh1285I CGRYCG 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 499, 964
BsiSI CCGG 6 cut(s) 357, 517, 766, 877, 995, 1133
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 1 cut(s) 883
BsmAI GTCTC 3 cut(s) 293, 651, 1119
BsmBI CGTCTC 2 cut(s) 293, 1119
BsmFI GGGAC 1 cut(s) 776
Bsp1286I GDGCHC 2 cut(s) 499, 964
Bsp13I TCCGGA 1 cut(s) 994
Bsp1407I TGTACA 1 cut(s) 148
Bsp143I GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 3 cut(s) 395, 1016, 1027
BspCNI CTCAG 4 cut(s) 376, 503, 718, 978
BspEI TCCGGA 1 cut(s) 994
BspFNI CGCG 1 cut(s) 1027
BspLI GGNNCC 1 cut(s) 764
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 1 cut(s) 898
BspQI GCTCTTC 1 cut(s) 952
BsrGI TGTACA 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
BssT1I CCWWGG 1 cut(s) 59
Bst4CI ACNGT 9 cut(s) 291, 304, 335, 416, 452, 540, 601, 712, 1046
Bst6I CTCTTC 1 cut(s) 952
BstAUI TGTACA 1 cut(s) 148
BstDEI CTNAG 7 cut(s) 384, 432, 490, 675, 726, 937, 986
BstF5I GGATG 5 cut(s) 28, 54, 226, 892, 991
BstFNI CGCG 1 cut(s) 1027
BstKTI GATC 7 cut(s) 297, 409, 471, 531, 674, 692, 893
BstMAI GTCTC 3 cut(s) 293, 651, 1119
BstMBI GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
BstMCI CGRYCG 1 cut(s) 297
BstPAI GACNNNNGTC 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 945
BstUI CGCG 1 cut(s) 1027
BstV1I GCAGC 2 cut(s) 568, 931
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 2 cut(s) 671, 689
BstYI RGATCY 2 cut(s) 671, 689
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 5 cut(s) 28, 54, 226, 892, 991
BtsI GCAGTG 1 cut(s) 1260
BtsIMutI CAGTG 2 cut(s) 234, 1260
Cfr13I GGNCC 2 cut(s) 763, 768
CseI GACGC 2 cut(s) 259, 437
Csp6I GTAC 2 cut(s) 149, 795
CviAII CATG 7 cut(s) 210, 217, 230, 683, 827, 1087, 1317
CviQI GTAC 2 cut(s) 149, 795
DdeI CTNAG 7 cut(s) 384, 432, 490, 675, 726, 937, 986
DpnI GATC 7 cut(s) 296, 408, 470, 530, 673, 691, 892
DpnII GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
Eam1104I CTCTTC 1 cut(s) 952
EarI CTCTTC 1 cut(s) 952
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 499
Eco32I GATATC 2 cut(s) 522, 1071
Eco47I GGWCC 2 cut(s) 763, 768
Eco53kI GAGCTC 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 75, 867
EcoICRI GAGCTC 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 866
EcoRV GATATC 2 cut(s) 522, 1071
EcoT14I CCWWGG 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 59
Esp3I CGTCTC 2 cut(s) 293, 1119
FaeI CATG 7 cut(s) 213, 220, 233, 686, 830, 1090, 1320
FaqI GGGAC 1 cut(s) 776
FatI CATG 7 cut(s) 209, 216, 229, 682, 826, 1086, 1316
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 582, 945
FokI GGATG 5 cut(s) 35, 61, 233, 899, 978
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 2 cut(s) 582, 945
FspBI CTAG 2 cut(s) 965, 1013
GluI GCNGC 2 cut(s) 582, 945
HapII CCGG 6 cut(s) 357, 517, 766, 877, 995, 1133
HgaI GACGC 2 cut(s) 259, 437
Hin1II CATG 7 cut(s) 213, 220, 233, 686, 830, 1090, 1320
HincII GTYRAC 4 cut(s) 354, 427, 574, 801
HindII GTYRAC 4 cut(s) 354, 427, 574, 801
HindIII AAGCTT 3 cut(s) 35, 186, 773
HinfI GANTC 7 cut(s) 169, 369, 502, 509, 605, 928, 1151
HpaI GTTAAC 3 cut(s) 354, 574, 801
HpaII CCGG 6 cut(s) 357, 517, 766, 877, 995, 1133
HphI GGTGA 2 cut(s) 506, 885
Hpy166II GTNNAC 9 cut(s) 307, 331, 354, 377, 427, 574, 629, 801, 1010
Hpy188I TCNGA 8 cut(s) 202, 385, 406, 501, 717, 907, 1037, 1050
Hpy188III TCNNGA 7 cut(s) 120, 274, 298, 492, 506, 761, 995
Hpy8I GTNNAC 9 cut(s) 307, 331, 354, 377, 427, 574, 629, 801, 1010
HpyAV CCTTC 4 cut(s) 4, 527, 1186, 1213
HpyCH4III ACNGT 9 cut(s) 291, 304, 335, 416, 452, 540, 601, 712, 1046
HpyCH4IV ACGT 3 cut(s) 549, 625, 797
HpyCH4V TGCA 4 cut(s) 584, 616, 947, 1118
HpyF3I CTNAG 7 cut(s) 384, 432, 490, 675, 726, 937, 986
HpySE526I ACGT 3 cut(s) 549, 625, 797
Hsp92II CATG 7 cut(s) 213, 220, 233, 686, 830, 1090, 1320
Kpn2I TCCGGA 1 cut(s) 994
KspAI GTTAAC 3 cut(s) 354, 574, 801
Kzo9I GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
LguI GCTCTTC 1 cut(s) 952
LmnI GCTCC 2 cut(s) 494, 502
LpnPI CCDG 8 cut(s) 278, 370, 477, 530, 779, 890, 1008, 1146
Lsp1109I GCAGC 2 cut(s) 568, 931
LweI GCATC 4 cut(s) 603, 1000, 1105, 1188
MaeI CTAG 2 cut(s) 965, 1013
MaeII ACGT 3 cut(s) 549, 625, 797
MaeIII GTNAC 7 cut(s) 232, 595, 706, 734, 1021, 1054, 1108
MalI GATC 7 cut(s) 296, 408, 470, 530, 673, 691, 892
MboI GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
MboII GAAGA 7 cut(s) 81, 135, 189, 662, 860, 969, 1244
MflI RGATCY 2 cut(s) 671, 689
MhlI GDGCHC 2 cut(s) 499, 964
MluCI AATT 5 cut(s) 153, 254, 718, 866, 1142
MnlI CCTC 6 cut(s) 660, 723, 909, 934, 961, 981
Mph1103I ATGCAT 1 cut(s) 618
MroI TCCGGA 1 cut(s) 994
MseI TTAA 9 cut(s) 180, 242, 338, 353, 542, 573, 747, 800, 1324
MslI CAYNNNNRTG 1 cut(s) 885
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 6 cut(s) 357, 517, 766, 877, 995, 1133
MvnI CGCG 1 cut(s) 1027
NdeII GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
NlaIII CATG 7 cut(s) 213, 220, 233, 686, 830, 1090, 1320
NlaIV GGNNCC 1 cut(s) 764
NmuCI GTSAC 2 cut(s) 232, 706
NsiI ATGCAT 1 cut(s) 618
PciSI GCTCTTC 1 cut(s) 952
PfeI GAWTC 7 cut(s) 169, 369, 502, 509, 605, 928, 1151
PkrI GCNGC 2 cut(s) 583, 946
Ple19I CGATCG 1 cut(s) 297
PshAI GACNNNNGTC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 499
Psp1406I AACGTT 1 cut(s) 549
PspN4I GGNNCC 1 cut(s) 764
PspPI GGNCC 2 cut(s) 763, 768
PstI CTGCAG 1 cut(s) 949
PsuI RGATCY 2 cut(s) 671, 689
PvuI CGATCG 1 cut(s) 297
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 2 cut(s) 150, 796
RsaNI GTAC 2 cut(s) 149, 795
RseI CAYNNNNRTG 1 cut(s) 885
SacI GAGCTC 1 cut(s) 499
SapI GCTCTTC 1 cut(s) 952
SaqAI TTAA 9 cut(s) 180, 242, 338, 353, 542, 573, 747, 800, 1324
SatI GCNGC 2 cut(s) 582, 945
Sau3AI GATC 7 cut(s) 294, 406, 468, 528, 671, 689, 890
Sau96I GGNCC 2 cut(s) 763, 768
SduI GDGCHC 2 cut(s) 499, 964
SfaNI GCATC 4 cut(s) 603, 1000, 1105, 1188
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 2 cut(s) 763, 768
SmiMI CAYNNNNRTG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 5 cut(s) 153, 254, 718, 866, 1142
SsiI CCGC 3 cut(s) 395, 1016, 1027
SspMI CTAG 2 cut(s) 965, 1013
SstI GAGCTC 1 cut(s) 499
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 9 cut(s) 291, 304, 335, 416, 452, 540, 601, 712, 1046
TaiI ACGT 3 cut(s) 552, 628, 800
TaqI TCGA 8 cut(s) 72, 293, 297, 507, 531, 608, 621, 783
TaqII GACCGA 1 cut(s) 381
TasI AATT 5 cut(s) 153, 254, 718, 866, 1142
TatI WGTACW 1 cut(s) 148
TfiI GAWTC 7 cut(s) 169, 369, 502, 509, 605, 928, 1151
Tru1I TTAA 9 cut(s) 180, 242, 338, 353, 542, 573, 747, 800, 1324
Tru9I TTAA 9 cut(s) 180, 242, 338, 353, 542, 573, 747, 800, 1324
TscAI CASTG 2 cut(s) 241, 1260
TseFI GTSAC 2 cut(s) 232, 706
TseI GCWGC 2 cut(s) 581, 944
Tsp45I GTSAC 2 cut(s) 232, 706
TspDTI ATGAA 7 cut(s) 23, 45, 65, 218, 623, 743, 815
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 2 cut(s) 241, 1260
VpaK11BI GGWCC 2 cut(s) 763, 768
XapI RAATTY 2 cut(s) 153, 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 1255
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 2 cut(s) 965, 1013
Zsp2I ATGCAT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.