RLG00000003697

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012060
Physical Location & Seq
Reverse (-)
51760189 .. 51762370
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003697

Sequence Viewer

Length: 1215 bp
ATGGAGTGGACTGCTTGTCTTGATGAATATGAGAAACTCGTCTTCCGAATGAACACCCCTAGGGTCGTGATTGACAATGCCGTCTGCTCCACTGCGACTTTAGTCAAGGTTTTTTTCCATGTTACTGACCAAAATGGGAACAAATTAACGGACGAGAGCGTTATCAATTACATTGAGCAGTCACTCGGAACGATTCACTTCGGGACCTCCAACTGTTCTAACGGCCTAACAGCCCTAGAGCTAACCGGAACGGACCGAGTTGGTCTTCTTTCCGAGGTCTTTGCTGTGCTGGCTGAGATGCAATGCAATGTGGTTGAGTCCAAGGTATGGACACACAACGGCCGAATTGCTTCACTGATTTATGTCAAGGACTGTGGTTCGGGTTGCCCAATTGAGGACTCGCAGAAAATTGATACAATTGAAGCGCGGTTGAGGACTGTGCTCAAGGGAGACAATGACATTCGGAGTGCGAAAACCTCAGTTTCCATGGCTGTCACGCACACCGAAAGAAGACTCCATCAGATGATGTTTGCAGATCGCGATTATGAAAGGAAGCCCATCCTACAGCTTTGCACTGATTCTCCAATTGTAACGGTGCAAAATTGGGCTGAAAGGAGCTATTCTGTAGTGAATATTCAGTGCAAGGATCGGGCAAAGCTTTTGTTTGATGTTGTTTGCACAATGACTGATATGGACTATGTGGTTTTCCATGCCACGATTAAAACAGCGGGAGATAGAGCACACATGGAATTTTACATTAGGCATACTGATGGAACCCCAATTAGTTCTGAACCCGAACGACATCGTGTAATTCAATGCTTACAAGCTGCCATTGAAAGAAGAGCATCTGAGGGTGTGAGACTTGAACTGTGCACCAAGGATAGAGAAGGTCTTCTAGCAAATGTCACCAGAACATTCCGAGAGGCCGGTCTCAATGTGACAAGGGCCGAGATATCCACCTCAACAGACAAGGCTCTAAACGTTTTCTATGTCACCGATGCAATTGGAAACCCTGCGGATCCAGAAACCATTGATGCAGTGAGGCAGAAAGTAGGGTTGAGTGACTTGAAAGTGAAGGAACTGCCATCCGTTTGCCATTCAGAGGGTGACAGAGAAGAGCAAGCACTAGGGGTTGGTGGGGCAGTGCTGTTGTCACTTGGGAGCTTAGTGAGAAGGAATCTATACAATTTGGGACTCATCAGGTCTTTTTCTTGA

Protein Analysis

405

Amino Acids

45.0

Weight (kDa)

5.76

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 78 - 124 2.5e-06 ACT domain
ACT_6 PF13740 80 - 116 4e-06 ACT domain
ACT_ACR9_3rd PF24931 197 - 278 1.3e-08 ACR9-like, ACT-like domain
ACT PF01842 209 - 261 4.5e-06 ACT domain
ACT PF01842 287 - 348 6.4e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 327
AccII CGCG 2 cut(s) 427, 540
AciI CCGC 3 cut(s) 427, 728, 1016
AclI AACGTT 1 cut(s) 981
AclWI GGATC 3 cut(s) 654, 1013, 1026
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 1 cut(s) 749
AfiI CCNNNNNNNGG 3 cut(s) 327, 394, 1102
AgsI TTSAA 5 cut(s) 422, 815, 836, 866, 1069
AhdI GACNNNNNGTC 1 cut(s) 15
AluBI AGCT 6 cut(s) 241, 568, 618, 658, 827, 1164
AluI AGCT 6 cut(s) 241, 568, 618, 658, 827, 1164
Alw21I GWGCWC 3 cut(s) 444, 742, 875
Alw26I GTCTC 3 cut(s) 444, 853, 935
Alw44I GTGCAC 1 cut(s) 871
AlwI GGATC 3 cut(s) 654, 1013, 1026
AoxI GGCC 4 cut(s) 223, 340, 924, 945
ApaLI GTGCAC 1 cut(s) 871
ApeKI GCWGC 1 cut(s) 827
ApoI RAATTY 1 cut(s) 749
Asp700I GAANNNNTTC 2 cut(s) 349, 891
AspA2I CCTAGG 1 cut(s) 59
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 3 cut(s) 204, 253, 945
AsuHPI GGTGA 3 cut(s) 898, 985, 1118
AvaII GGWCC 2 cut(s) 204, 253
AvrII CCTAGG 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 875
BamHI GGATCC 1 cut(s) 1018
BbsI GAAGAC 4 cut(s) 34, 257, 517, 884
Bbv12I GWGCWC 3 cut(s) 444, 742, 875
BbvI GCAGC 1 cut(s) 814
BccI CCATC 4 cut(s) 525, 566, 764, 1093
BceAI ACGGC 3 cut(s) 65, 238, 355
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BcoDI GTCTC 3 cut(s) 444, 853, 935
BfaI CTAG 4 cut(s) 60, 236, 896, 1127
BfmI CTRYAG 2 cut(s) 563, 624
BisI GCNGC 1 cut(s) 828
BlnI CCTAGG 1 cut(s) 59
BlsI GCNGC 1 cut(s) 829
Bme18I GGWCC 2 cut(s) 204, 253
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 3 cut(s) 204, 253, 945
BmiI GGNNCC 3 cut(s) 205, 775, 1020
BmsI GCATC 4 cut(s) 288, 854, 988, 1024
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 4 cut(s) 34, 257, 517, 884
BpuEI CTTGAG 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 935
BsaJI CCNNGG 5 cut(s) 59, 273, 321, 486, 876
BsaWI WCCGGW 1 cut(s) 245
BsaXI ACNNNNNCTCC 2 cut(s) 565, 595
Bsc4I CCNNNNNNNGG 3 cut(s) 327, 394, 1102
Bse118I RCCGGY 1 cut(s) 926
Bse3DI GCAATG 2 cut(s) 308, 313
BseDI CCNNGG 5 cut(s) 59, 273, 321, 486, 876
BseGI GGATG 2 cut(s) 558, 1085
BseLI CCNNNNNNNGG 3 cut(s) 327, 394, 1102
BseMI GCAATG 2 cut(s) 308, 313
BseMII CTCAG 3 cut(s) 285, 492, 840
BseSI GKGCMC 1 cut(s) 875
BseX3I CGGCCG 1 cut(s) 340
BseXI GCAGC 1 cut(s) 814
Bsh1236I CGCG 2 cut(s) 427, 540
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 4 cut(s) 225, 342, 926, 947
BsiEI CGRYCG 1 cut(s) 343
BsiHKAI GWGCWC 3 cut(s) 444, 742, 875
BsiSI CCGG 2 cut(s) 246, 927
BslFI GGGAC 2 cut(s) 217, 1206
BslI CCNNNNNNNGG 3 cut(s) 327, 394, 1102
BsmAI GTCTC 3 cut(s) 444, 853, 935
BsmFI GGGAC 2 cut(s) 217, 1206
BsnI GGCC 4 cut(s) 225, 342, 926, 947
Bso31I GGTCTC 1 cut(s) 935
Bsp1286I GDGCHC 3 cut(s) 444, 742, 875
Bsp143I GATC 3 cut(s) 535, 646, 1018
Bsp19I CCATGG 1 cut(s) 486
Bsp68I TCGCGA 1 cut(s) 540
BspACI CCGC 3 cut(s) 427, 728, 1016
BspANI GGCC 4 cut(s) 225, 342, 926, 947
BspCNI CTCAG 3 cut(s) 286, 491, 841
BspFNI CGCG 2 cut(s) 427, 540
BspLI GGNNCC 3 cut(s) 205, 775, 1020
BspPI GGATC 3 cut(s) 654, 1013, 1026
BspQI GCTCTTC 2 cut(s) 835, 1110
BspTNI GGTCTC 1 cut(s) 935
BsrDI GCAATG 2 cut(s) 308, 313
BsrFI RCCGGY 1 cut(s) 926
BssAI RCCGGY 1 cut(s) 926
BssECI CCNNGG 5 cut(s) 59, 273, 321, 486, 876
BssMI GATC 3 cut(s) 535, 646, 1018
BssT1I CCWWGG 4 cut(s) 59, 321, 486, 876
Bst4CI ACNGT 5 cut(s) 215, 374, 439, 595, 870
Bst6I CTCTTC 2 cut(s) 835, 1110
BstC8I GCNNGC 2 cut(s) 291, 1122
BstDEI CTNAG 4 cut(s) 294, 478, 849, 1165
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 2 cut(s) 558, 1085
BstFNI CGCG 2 cut(s) 427, 540
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 3 cut(s) 538, 649, 1021
BstMAI GTCTC 3 cut(s) 444, 853, 935
BstMBI GATC 3 cut(s) 535, 646, 1018
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstPAI GACNNNNGTC 1 cut(s) 101
BstSFI CTRYAG 2 cut(s) 563, 624
BstSLI GKGCMC 1 cut(s) 875
BstUI CGCG 2 cut(s) 427, 540
BstV1I GCAGC 1 cut(s) 814
BstV2I GAAGAC 4 cut(s) 34, 257, 517, 884
BstX2I RGATCY 1 cut(s) 1018
BstYI RGATCY 1 cut(s) 1018
BstZI CGGCCG 1 cut(s) 340
BsuRI GGCC 4 cut(s) 225, 342, 926, 947
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 558, 1085
BtsI GCAGTG 3 cut(s) 90, 1044, 1149
BtsIMutI CAGTG 6 cut(s) 90, 353, 573, 644, 1044, 1149
BtuMI TCGCGA 1 cut(s) 540
Cac8I GCNNGC 2 cut(s) 291, 1122
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 926
Cfr13I GGNCC 3 cut(s) 204, 253, 945
CpoI CGGWCCG 1 cut(s) 253
CspCI CAANNNNNGTGG 2 cut(s) 355, 390
CspI CGGWCCG 1 cut(s) 253
CviAII CATG 4 cut(s) 119, 487, 710, 745
DdeI CTNAG 4 cut(s) 294, 478, 849, 1165
DpnI GATC 3 cut(s) 537, 648, 1020
DpnII GATC 3 cut(s) 535, 646, 1018
DrdI GACNNNNNNGTC 1 cut(s) 80
DriI GACNNNNNGTC 1 cut(s) 15
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 340
EagI CGGCCG 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 835, 1110
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 2 cut(s) 835, 1110
EclXI CGGCCG 1 cut(s) 340
Eco130I CCWWGG 4 cut(s) 59, 321, 486, 876
Eco31I GGTCTC 1 cut(s) 935
Eco32I GATATC 1 cut(s) 954
Eco47I GGWCC 2 cut(s) 204, 253
Eco52I CGGCCG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 204
EcoRV GATATC 1 cut(s) 954
EcoT14I CCWWGG 4 cut(s) 59, 321, 486, 876
ErhI CCWWGG 4 cut(s) 59, 321, 486, 876
FaeI CATG 4 cut(s) 122, 490, 713, 748
FaqI GGGAC 2 cut(s) 217, 1206
FatI CATG 4 cut(s) 118, 486, 709, 744
FauI CCCGC 1 cut(s) 721
Fnu4HI GCNGC 1 cut(s) 828
FokI GGATG 2 cut(s) 545, 1072
Fsp4HI GCNGC 1 cut(s) 828
FspBI CTAG 4 cut(s) 60, 236, 896, 1127
GlaI GCGC 1 cut(s) 426
GluI GCNGC 1 cut(s) 828
HaeIII GGCC 4 cut(s) 225, 342, 926, 947
HapII CCGG 2 cut(s) 246, 927
HhaI GCGC 1 cut(s) 427
Hin1II CATG 4 cut(s) 122, 490, 713, 748
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 7 cut(s) 193, 317, 398, 513, 578, 1177, 1194
HpaII CCGG 2 cut(s) 246, 927
HphI GGTGA 3 cut(s) 898, 985, 1118
Hpy166II GTNNAC 2 cut(s) 9, 873
Hpy188I TCNGA 9 cut(s) 47, 188, 274, 465, 522, 790, 850, 920, 1102
Hpy188III TCNNGA 6 cut(s) 20, 67, 202, 539, 1022, 1212
Hpy8I GTNNAC 2 cut(s) 9, 873
HpyAV CCTTC 3 cut(s) 881, 1069, 1167
HpyCH4III ACNGT 5 cut(s) 215, 374, 439, 595, 870
HpyCH4IV ACGT 1 cut(s) 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 4 cut(s) 294, 478, 849, 1165
HpySE526I ACGT 1 cut(s) 981
Hsp92II CATG 4 cut(s) 122, 490, 713, 748
HspAI GCGC 1 cut(s) 425
Kzo9I GATC 3 cut(s) 535, 646, 1018
LguI GCTCTTC 2 cut(s) 835, 1110
LmnI GCTCC 3 cut(s) 92, 615, 1161
LpnPI CCDG 7 cut(s) 259, 275, 922, 940, 1026, 1035, 1186
Lsp1109I GCAGC 1 cut(s) 814
LweI GCATC 4 cut(s) 288, 854, 988, 1024
MaeI CTAG 4 cut(s) 60, 236, 896, 1127
MaeII ACGT 1 cut(s) 981
MalI GATC 3 cut(s) 537, 648, 1020
MboI GATC 3 cut(s) 535, 646, 1018
MboII GAAGA 6 cut(s) 34, 257, 522, 852, 884, 1127
MfeI CAATTG 4 cut(s) 390, 417, 585, 1002
MflI RGATCY 1 cut(s) 1018
MhlI GDGCHC 3 cut(s) 444, 742, 875
MlyI GAGTC 4 cut(s) 326, 392, 507, 1188
MmeI TCCRAC 1 cut(s) 234
MroXI GAANNNNTTC 2 cut(s) 349, 891
MseI TTAA 2 cut(s) 146, 720
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 2 cut(s) 246, 927
MunI CAATTG 4 cut(s) 390, 417, 585, 1002
MvnI CGCG 2 cut(s) 427, 540
MwoI GCNNNNNNNGC 1 cut(s) 290
NcoI CCATGG 1 cut(s) 486
NdeII GATC 3 cut(s) 535, 646, 1018
NlaIII CATG 4 cut(s) 122, 490, 713, 748
NlaIV GGNNCC 3 cut(s) 205, 775, 1020
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 8 cut(s) 180, 493, 904, 937, 991, 1061, 1106, 1152
NruI TCGCGA 1 cut(s) 540
PciSI GCTCTTC 2 cut(s) 835, 1110
PdmI GAANNNNTTC 2 cut(s) 349, 891
PfeI GAWTC 3 cut(s) 193, 578, 1177
PflMI CCANNNNNTGG 1 cut(s) 327
PkrI GCNGC 1 cut(s) 829
PleI GAGTC 4 cut(s) 325, 392, 507, 1188
PpsI GAGTC 4 cut(s) 325, 392, 507, 1188
PpuMI RGGWCCY 1 cut(s) 204
PshAI GACNNNNGTC 1 cut(s) 101
Psp1406I AACGTT 1 cut(s) 981
Psp5II RGGWCCY 1 cut(s) 204
PspN4I GGNNCC 3 cut(s) 205, 775, 1020
PspPI GGNCC 3 cut(s) 204, 253, 945
PspPPI RGGWCCY 1 cut(s) 204
PsuI RGATCY 1 cut(s) 1018
RruI TCGCGA 1 cut(s) 540
RseI CAYNNNNRTG 1 cut(s) 768
Rsr2I CGGWCCG 1 cut(s) 253
RsrII CGGWCCG 1 cut(s) 253
SapI GCTCTTC 2 cut(s) 835, 1110
SaqAI TTAA 2 cut(s) 146, 720
SatI GCNGC 1 cut(s) 828
Sau3AI GATC 3 cut(s) 535, 646, 1018
Sau96I GGNCC 3 cut(s) 204, 253, 945
SchI GAGTC 4 cut(s) 326, 392, 507, 1188
SduI GDGCHC 3 cut(s) 444, 742, 875
SfaNI GCATC 4 cut(s) 288, 854, 988, 1024
SfcI CTRYAG 2 cut(s) 563, 624
SinI GGWCC 2 cut(s) 204, 253
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
SsiI CCGC 3 cut(s) 427, 728, 1016
SspI AATATT 1 cut(s) 634
SspMI CTAG 4 cut(s) 60, 236, 896, 1127
StyI CCWWGG 4 cut(s) 59, 321, 486, 876
TaaI ACNGT 5 cut(s) 215, 374, 439, 595, 870
TaiI ACGT 1 cut(s) 984
TaqII GACCGA 1 cut(s) 270
TfiI GAWTC 3 cut(s) 193, 578, 1177
Tru1I TTAA 2 cut(s) 146, 720
Tru9I TTAA 2 cut(s) 146, 720
TscAI CASTG 6 cut(s) 97, 360, 580, 644, 1044, 1149
TseFI GTSAC 8 cut(s) 180, 493, 904, 937, 991, 1061, 1106, 1152
TseI GCWGC 1 cut(s) 827
Tsp45I GTSAC 8 cut(s) 180, 493, 904, 937, 991, 1061, 1106, 1152
TspDTI ATGAA 3 cut(s) 39, 65, 561
TspGWI ACGGA 3 cut(s) 164, 266, 1078
TspRI CASTG 6 cut(s) 97, 360, 580, 644, 1044, 1149
Van91I CCANNNNNTGG 1 cut(s) 327
VneI GTGCAC 1 cut(s) 871
VpaK11BI GGWCC 2 cut(s) 204, 253
XapI RAATTY 1 cut(s) 749
XmaJI CCTAGG 1 cut(s) 59
XmnI GAANNNNTTC 2 cut(s) 349, 891
XspI CTAG 4 cut(s) 60, 236, 896, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.