pycom04g06870

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012060
Physical Location & Seq
Forward (+)
7153154 .. 7155479
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06870.6

Sequence Viewer

Length: 1332 bp
ATGGAGTGTACTGCTTACCTCGACGAATATGAAAAACTCGTCTTCCGGATGAACACTCCCAGGGTCGTGATCGACAATGCCGTCTGCTCCACTGCGACTTTAGTCAAGGTTGACAGTGCTAGAAGGCATGGAATTCTTCTGGATGCTGTGCAAGTCCTCACGGATCTTAATCTCTCCATTAAAAAAGCTTACATTTCTTCTGATGGTCGCTGGTTCATGGATGTTTTTCATGTTACTGATCAAAACGGAAATAAATTGACGGACGAGAGCGTTATTAACTATATTGAGCAGTCACTTGGAACAATTCATTTTGGATCATCCAACTGTATTAAGGGTCTTACGGCCCTTGAGCTTACCGGGACAGATCGAGTAGGCCTTCTTTCCGAGGTGTTTGCAGTGCTAGCTGACCTGCAATGCAATGTGGTTGAGTCCAAGGTGTGGACGCACAACGGCCGTATTGCTTCACTGATATACGTGAAGGACTGTGATTCCGGGTGCCCTATTGAGGACTCGCAGAAAATTGATAGAATTGAAGCGCGGTTAAGGAATGTTCTCAAGGGCGACAATGATATTAGGAGTGCGAAAACCTCTGTTTCCATGGCGGTTACACACACCGAAAGAAGACTGCATCAAATGATGTTTGCTGATCGTGATTATGATCGAAAGCCTGTTTTGCAGCTTTGCACCGATTCTCCTGTCATAACGGTGCAGAATTGGGCGGAAAGGGGTTACTCCATAGTGAATATTCAGTGCAAGGATAGGGCAAAGCTTTTGTTCGATGTTGTTTGCACATTGACAGACATGGACTATGTGGTTTTTCATGCCACTATTAAAACATCAGGGGACAGAGGATACATGGAATTTTACATCAGGCACACTGATGGAACCCCGATTAGCTCTGAACCCGAACGACAGCGTGTAATCCAATGCTTACAAGCCGCGGTTCAAAGAAGGGCATCCGAGGGTGTGAGGCTAGAACTATGCACCGAGGATAGGCAAGGTCTTCTAGCATACGTTACAAGAACATTCCGCGAGAACGGTCTTAATGTGACAAGGGCCGAGATTTCCACAACGATGGACAAAGCTCTAAACGTATTCTACGTGACAGATGCGGTCGGAAACCCTGCAGATCCGAAAACCATCGAATCAGTGAGGCAGAAAATCGGGTTGAGTAACTTGAAAGTGAAGGAACTGCCATTGATATACCATCCAAAGGAGGAAGGGGAGGAGCAGCAAGGAGTTGGTGGTGTTGGTGGGGCAGTGTTGTTTTCACTTGGGAGCCTAGTGAGAAGGAATCTGTACAATTTGGGGCTCATCAGATCTCATTCTTGA

Protein Analysis

444

Amino Acids

49.62

Weight (kDa)

6.24

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 495
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 3 cut(s) 538, 941, 1032
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 7 cut(s) 538, 602, 719, 939, 941, 1030, 1112
AclWI GGATC 3 cut(s) 171, 322, 1124
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 2 cut(s) 132, 860
AfaI GTAC 2 cut(s) 10, 1301
AfiI CCNNNNNNNGG 4 cut(s) 438, 505, 993, 1213
AgsI TTSAA 3 cut(s) 533, 947, 1180
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 7 cut(s) 188, 352, 404, 679, 769, 897, 1085
AluI AGCT 7 cut(s) 188, 352, 404, 679, 769, 897, 1085
AlwI GGATC 3 cut(s) 171, 322, 1124
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 4 cut(s) 342, 373, 451, 1056
ApeKI GCWGC 2 cut(s) 676, 1231
ApoI RAATTY 2 cut(s) 132, 860
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 2 cut(s) 343, 1056
AsuC2I CCSGG 2 cut(s) 358, 493
AsuNHI GCTAGC 1 cut(s) 400
BaeGI GKGCMC 1 cut(s) 500
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 1314
BbsI GAAGAC 3 cut(s) 34, 628, 995
BbvI GCAGC 2 cut(s) 688, 1243
BccI CCATC 5 cut(s) 197, 875, 1069, 1148, 1215
BceAI ACGGC 4 cut(s) 65, 357, 438, 466
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 845
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 2 cut(s) 358, 493
BfaI CTAG 5 cut(s) 120, 401, 974, 1007, 1283
BfmI CTRYAG 1 cut(s) 1125
BfuAI ACCTGC 1 cut(s) 417
BfuI GTATCC 1 cut(s) 845
BglII AGATCT 1 cut(s) 1319
BisI GCNGC 3 cut(s) 677, 939, 1232
BlsI GCNGC 3 cut(s) 678, 940, 1233
Bme1390I CCNGG 3 cut(s) 61, 358, 493
BmgT120I GGNCC 2 cut(s) 343, 1056
BmiI GGNNCC 3 cut(s) 497, 886, 1280
BmrFI CCNGG 3 cut(s) 61, 358, 493
BmsI GCATC 4 cut(s) 133, 637, 965, 1099
BmtI GCTAGC 1 cut(s) 404
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 3 cut(s) 34, 628, 995
BpuEI CTTGAG 2 cut(s) 368, 539
BpuMI CCSGG 2 cut(s) 358, 493
BsaAI YACGTR 2 cut(s) 475, 1102
BsaBI GATNNNNATC 2 cut(s) 168, 657
BsaJI CCNNGG 8 cut(s) 59, 60, 384, 432, 597, 939, 960, 987
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 676, 706
Bsc4I CCNNNNNNNGG 4 cut(s) 438, 505, 993, 1213
Bse3DI GCAATG 2 cut(s) 419, 424
Bse8I GATNNNNATC 2 cut(s) 168, 657
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 8 cut(s) 59, 60, 384, 432, 597, 939, 960, 987
BseGI GGATG 6 cut(s) 54, 148, 226, 317, 956, 1207
BseJI GATNNNNATC 2 cut(s) 168, 657
BseLI CCNNNNNNNGG 4 cut(s) 438, 505, 993, 1213
BseMI GCAATG 2 cut(s) 419, 424
BseRI GAGGAG 1 cut(s) 1241
BseSI GKGCMC 1 cut(s) 500
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 2 cut(s) 688, 1243
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 3 cut(s) 538, 941, 1032
Bsh1285I CGRYCG 2 cut(s) 454, 1116
BshFI GGCC 4 cut(s) 344, 375, 453, 1058
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 2 cut(s) 454, 1116
BsiSI CCGG 3 cut(s) 46, 357, 492
BslFI GGGAC 2 cut(s) 373, 857
BslI CCNNNNNNNGG 4 cut(s) 438, 505, 993, 1213
BsmFI GGGAC 2 cut(s) 373, 857
BsnI GGCC 4 cut(s) 344, 375, 453, 1058
Bsp1286I GDGCHC 2 cut(s) 500, 1314
Bsp13I TCCGGA 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 1299
Bsp143I GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 7 cut(s) 538, 602, 719, 939, 941, 1030, 1112
BspANI GGCC 4 cut(s) 344, 375, 453, 1058
BspEI TCCGGA 1 cut(s) 45
BspFNI CGCG 3 cut(s) 538, 941, 1032
BspLI GGNNCC 3 cut(s) 497, 886, 1280
BspMAI CTGCAG 1 cut(s) 1129
BspMI ACCTGC 1 cut(s) 417
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 3 cut(s) 171, 322, 1124
BspT107I GGYRCC 1 cut(s) 495
BsrDI GCAATG 2 cut(s) 419, 424
BsrGI TGTACA 1 cut(s) 1299
BssECI CCNNGG 8 cut(s) 59, 60, 384, 432, 597, 939, 960, 987
BssMI GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
BssT1I CCWWGG 2 cut(s) 432, 597
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 5 cut(s) 116, 326, 485, 706, 1040
BstAUI TGTACA 1 cut(s) 1299
BstBAI YACGTR 2 cut(s) 475, 1102
BstC8I GCNNGC 1 cut(s) 402
BstDSI CCRYGG 2 cut(s) 597, 939
BstF5I GGATG 6 cut(s) 54, 148, 226, 317, 956, 1207
BstFNI CGCG 3 cut(s) 538, 941, 1032
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 9 cut(s) 72, 166, 241, 317, 367, 649, 661, 1132, 1322
BstMBI GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
BstMCI CGRYCG 2 cut(s) 454, 1116
BstMWI GCNNNNNNNGC 2 cut(s) 401, 673
BstNI CCWGG 1 cut(s) 61
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 3 cut(s) 59, 356, 491
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 500
BstUI CGCG 3 cut(s) 538, 941, 1032
BstV1I GCAGC 2 cut(s) 688, 1243
BstV2I GAAGAC 3 cut(s) 34, 628, 995
BstX2I RGATCY 3 cut(s) 163, 1129, 1319
BstXI CCANNNNNNTGG 1 cut(s) 1075
BstYI RGATCY 3 cut(s) 163, 1129, 1319
BstZI CGGCCG 1 cut(s) 451
BsuI GTATCC 1 cut(s) 845
BsuRI GGCC 4 cut(s) 344, 375, 453, 1058
BtgI CCRYGG 2 cut(s) 597, 939
BtsCI GGATG 6 cut(s) 54, 148, 226, 317, 956, 1207
BtsI GCAGTG 3 cut(s) 90, 402, 1266
BtsIMutI CAGTG 8 cut(s) 90, 121, 402, 464, 755, 876, 1155, 1266
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 402
CfoI GCGC 1 cut(s) 538
Cfr13I GGNCC 2 cut(s) 343, 1056
Cfr42I CCGCGG 1 cut(s) 942
CseI GACGC 1 cut(s) 451
Csp6I GTAC 2 cut(s) 9, 1300
CviAII CATG 7 cut(s) 128, 217, 230, 598, 802, 821, 856
CviQI GTAC 2 cut(s) 9, 1300
DpnI GATC 9 cut(s) 71, 165, 240, 316, 366, 648, 660, 1131, 1321
DpnII GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 451
EagI CGGCCG 1 cut(s) 451
EciI GGCGGA 1 cut(s) 734
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 432, 597
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 1314
Eco52I CGGCCG 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 432, 597
EcoT38I GRGCYC 1 cut(s) 1314
ErhI CCWWGG 2 cut(s) 432, 597
FaeI CATG 7 cut(s) 131, 220, 233, 601, 805, 824, 859
FaqI GGGAC 2 cut(s) 373, 857
FatI CATG 7 cut(s) 127, 216, 229, 597, 801, 820, 855
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 3 cut(s) 677, 939, 1232
FokI GGATG 6 cut(s) 61, 155, 233, 304, 943, 1194
FriOI GRGCYC 1 cut(s) 1314
Fsp4HI GCNGC 3 cut(s) 677, 939, 1232
FspBI CTAG 5 cut(s) 120, 401, 974, 1007, 1283
GlaI GCGC 1 cut(s) 537
GluI GCNGC 3 cut(s) 677, 939, 1232
HaeIII GGCC 4 cut(s) 344, 375, 453, 1058
HapII CCGG 3 cut(s) 46, 357, 492
HgaI GACGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 538
Hin1II CATG 7 cut(s) 131, 220, 233, 601, 805, 824, 859
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 2 cut(s) 186, 767
HinfI GANTC 6 cut(s) 428, 488, 509, 689, 1145, 1294
HpaII CCGG 3 cut(s) 46, 357, 492
Hpy166II GTNNAC 3 cut(s) 9, 112, 441
Hpy188I TCNGA 7 cut(s) 202, 385, 901, 961, 1118, 1134, 1319
Hpy188III TCNNGA 5 cut(s) 46, 67, 140, 650, 1329
Hpy8I GTNNAC 3 cut(s) 9, 112, 441
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 7 cut(s) 117, 386, 472, 945, 1180, 1214, 1284
HpyCH4III ACNGT 5 cut(s) 116, 326, 485, 706, 1040
HpyCH4IV ACGT 4 cut(s) 474, 1014, 1092, 1101
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 673
HpySE526I ACGT 4 cut(s) 474, 1014, 1092, 1101
Hsp92II CATG 7 cut(s) 131, 220, 233, 601, 805, 824, 859
HspAI GCGC 1 cut(s) 536
Kpn2I TCCGGA 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 238
KspI CCGCGG 1 cut(s) 942
Kzo9I GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
LmnI GCTCC 3 cut(s) 92, 1228, 1278
Lsp1109I GCAGC 2 cut(s) 688, 1243
LweI GCATC 4 cut(s) 133, 637, 965, 1099
MaeI CTAG 5 cut(s) 120, 401, 974, 1007, 1283
MaeII ACGT 4 cut(s) 474, 1014, 1092, 1101
MaeIII GTNAC 8 cut(s) 232, 291, 604, 728, 1015, 1048, 1102, 1172
MalI GATC 9 cut(s) 71, 165, 240, 316, 366, 648, 660, 1131, 1321
MboI GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
MboII GAAGA 5 cut(s) 34, 128, 189, 633, 995
MflI RGATCY 3 cut(s) 163, 1129, 1319
MhlI GDGCHC 2 cut(s) 500, 1314
MluCI AATT 8 cut(s) 132, 254, 303, 519, 528, 712, 860, 1303
MlyI GAGTC 2 cut(s) 437, 503
MmeI TCCRAC 2 cut(s) 345, 1096
MroI TCCGGA 1 cut(s) 45
MseI TTAA 7 cut(s) 168, 180, 276, 330, 542, 831, 1044
MslI CAYNNNNRTG 3 cut(s) 704, 879, 1073
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 3 cut(s) 46, 357, 492
MspR9I CCNGG 3 cut(s) 61, 358, 493
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 3 cut(s) 538, 941, 1032
MwoI GCNNNNNNNGC 2 cut(s) 401, 673
NciI CCSGG 2 cut(s) 358, 493
NcoI CCATGG 1 cut(s) 597
NdeII GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 7 cut(s) 131, 220, 233, 601, 805, 824, 859
NlaIV GGNNCC 3 cut(s) 497, 886, 1280
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 3 cut(s) 291, 1048, 1102
PasI CCCWGGG 1 cut(s) 60
PceI AGGCCT 1 cut(s) 375
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 1098
PfeI GAWTC 4 cut(s) 488, 689, 1145, 1294
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 3 cut(s) 678, 940, 1233
PleI GAGTC 2 cut(s) 436, 503
PpsI GAGTC 2 cut(s) 436, 503
Ppu21I YACGTR 2 cut(s) 475, 1102
PshAI GACNNNNGTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 3 cut(s) 497, 886, 1280
PspPI GGNCC 2 cut(s) 343, 1056
PstI CTGCAG 1 cut(s) 1129
PsuI RGATCY 3 cut(s) 163, 1129, 1319
RsaI GTAC 2 cut(s) 10, 1301
RsaNI GTAC 2 cut(s) 9, 1300
RseI CAYNNNNRTG 3 cut(s) 704, 879, 1073
SacII CCGCGG 1 cut(s) 942
SaqAI TTAA 7 cut(s) 168, 180, 276, 330, 542, 831, 1044
SatI GCNGC 3 cut(s) 677, 939, 1232
Sau3AI GATC 9 cut(s) 69, 163, 238, 314, 364, 646, 658, 1129, 1319
Sau96I GGNCC 2 cut(s) 343, 1056
SchI GAGTC 2 cut(s) 437, 503
ScrFI CCNGG 3 cut(s) 61, 358, 493
SduI GDGCHC 2 cut(s) 500, 1314
SfaNI GCATC 4 cut(s) 133, 637, 965, 1099
SfcI CTRYAG 1 cut(s) 1125
Sfr303I CCGCGG 1 cut(s) 942
SgrBI CCGCGG 1 cut(s) 942
SmiMI CAYNNNNRTG 3 cut(s) 704, 879, 1073
SmlI CTYRAG 2 cut(s) 347, 554
SmoI CTYRAG 2 cut(s) 347, 554
Sse9I AATT 8 cut(s) 132, 254, 303, 519, 528, 712, 860, 1303
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 7 cut(s) 538, 602, 719, 939, 941, 1030, 1112
SspI AATATT 1 cut(s) 745
SspMI CTAG 5 cut(s) 120, 401, 974, 1007, 1283
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 3 cut(s) 59, 356, 491
StyI CCWWGG 2 cut(s) 432, 597
TaaI ACNGT 5 cut(s) 116, 326, 485, 706, 1040
TaiI ACGT 4 cut(s) 477, 1017, 1095, 1104
TaqI TCGA 6 cut(s) 21, 72, 367, 661, 777, 1143
TasI AATT 8 cut(s) 132, 254, 303, 519, 528, 712, 860, 1303
TatI WGTACW 2 cut(s) 8, 1299
TauI GCSGC 1 cut(s) 941
TfiI GAWTC 4 cut(s) 488, 689, 1145, 1294
Tru1I TTAA 7 cut(s) 168, 180, 276, 330, 542, 831, 1044
Tru9I TTAA 7 cut(s) 168, 180, 276, 330, 542, 831, 1044
TscAI CASTG 8 cut(s) 97, 121, 402, 471, 755, 883, 1155, 1266
TseFI GTSAC 3 cut(s) 291, 1048, 1102
TseI GCWGC 2 cut(s) 676, 1231
Tsp45I GTSAC 3 cut(s) 291, 1048, 1102
TspDTI ATGAA 6 cut(s) 45, 65, 205, 218, 296, 809
TspGWI ACGGA 3 cut(s) 176, 261, 275
TspRI CASTG 8 cut(s) 97, 121, 402, 471, 755, 883, 1155, 1266
Van91I CCANNNNNTGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 132, 860
XspI CTAG 5 cut(s) 120, 401, 974, 1007, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.