Rh7AG187200

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012060
Physical Location & Seq
Forward (+)
16748365 .. 16751858
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG187200.1

Sequence Viewer

Length: 1110 bp
ATGGATGTTTTCCATGTTACTGACCAAAATGGGAACAAATTAACGGACGAGAGCGTTATCAATTACATTGAGCAGTCACTCGGAACGATTCACTTCGGGACCTCCAACTGTTCTAACGGCCTAACAGCCCTGGAGTTAACCGGAACGGACCGAGTTGGTCTTCTTTCCGAGGTCTTTGCAGTGCTGGCTGAGATGCAATGCAATGTGGTTGAGTCCAAGGTATGGACACACAACGGCCGAATTGCTTCACTGATTTATGTCAAGGACTGTGGTTCGGGTTGCCCAATTGAGGACTCGCAGAAAATTGATACAATTGAAGCACGGTTGAGGACTGTGCTCAAGGGAGACAATGACATTCGGAGTGCGAAAACCTCAGTTTCCATGGCTGTCACGCACACCGAAAGAAGACTCCATCAGATGATGTTTGCAGATCGCGATTATGAAAGGAAGCCCATCCTACAGCTTTGCACTGATTCTCCGATTGTAACGGTGCAGAATTGGGCTGAAAGGAGCTATTCTGTAGTGAATATTCAGTGCAAGGATCGGGCAAAGCTTTTGTTTGATGTTGTTTGCACAATGACAGATATGGACTATGTGGTTTTCCATGCCACGATTAAAACAGCGGGAGATAGAGCACACATGGAATTTTACATTAGGCATACTGATGGAACCCCAATTAGTTCGGAACCCGAACGACATCGTGTAATTCAATGCTTACAAGCTGCCATTGAAAGAAGAGCATCTGAGGGTGTGAGACTTGAATTGTGCACCCAGGATAGAGAAGGTCTTCTAGCAAATGTCACCAGAACATTCCGAGAGGCCGGTCTCAATGTGACAAGGGCTGAGATATCCACCTCAACAGACAAGGCTCTAAACGTTTTCTATGTCACCGATGCAATTGGAAACCCTGCGGATCCAGAAACCATTGATGCAGTGAGGCAGAAAGTAGGGTTGAGTGACTTGAAAGTGAAGGAATTGCCATCCGTTTGCCATTCAGAGGGTGACAGAGAAGAGCAAGCACTAGGGGTTGGTGGGGCAGTGCTGTTATCACTTGGGAGCTTAGTGAGAAGGAATCTATACAATTTGGGACTCATCAGGTCTTTTTCTTGA

Protein Analysis

369

Amino Acids

40.97

Weight (kDa)

5.64

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 43 - 89 2.2e-06 ACT domain
ACT_6 PF13740 45 - 81 3.5e-06 ACT domain
ACT_ACR9_3rd PF24931 162 - 244 1e-08 ACR9-like, ACT-like domain
ACT PF01842 174 - 226 4e-06 ACT domain
ACT PF01842 252 - 313 5.1e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 222
AccII CGCG 1 cut(s) 435
AciI CCGC 2 cut(s) 623, 911
AclI AACGTT 1 cut(s) 876
AclWI GGATC 3 cut(s) 549, 908, 921
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 1 cut(s) 644
AfiI CCNNNNNNNGG 3 cut(s) 222, 289, 997
AgsI TTSAA 5 cut(s) 317, 710, 731, 761, 964
AjnI CCWGG 2 cut(s) 129, 771
AluBI AGCT 5 cut(s) 463, 513, 553, 722, 1059
AluI AGCT 5 cut(s) 463, 513, 553, 722, 1059
Alw21I GWGCWC 3 cut(s) 339, 637, 770
Alw26I GTCTC 3 cut(s) 339, 748, 830
Alw44I GTGCAC 1 cut(s) 766
AlwI GGATC 3 cut(s) 549, 908, 921
AoxI GGCC 3 cut(s) 118, 235, 819
ApaLI GTGCAC 1 cut(s) 766
ApeKI GCWGC 1 cut(s) 722
ApoI RAATTY 1 cut(s) 644
Asp700I GAANNNNTTC 2 cut(s) 244, 786
AspS9I GGNCC 2 cut(s) 99, 148
AsuHPI GGTGA 3 cut(s) 793, 880, 1013
AvaII GGWCC 2 cut(s) 99, 148
BaeGI GKGCMC 1 cut(s) 770
BamHI GGATCC 1 cut(s) 913
BbsI GAAGAC 3 cut(s) 152, 412, 779
Bbv12I GWGCWC 3 cut(s) 339, 637, 770
BbvI GCAGC 1 cut(s) 709
BccI CCATC 4 cut(s) 420, 461, 659, 988
BceAI ACGGC 2 cut(s) 133, 250
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 2 cut(s) 131, 773
BcoDI GTCTC 3 cut(s) 339, 748, 830
BfaI CTAG 2 cut(s) 791, 1022
BfmI CTRYAG 2 cut(s) 458, 519
BisI GCNGC 1 cut(s) 723
BlsI GCNGC 1 cut(s) 724
Bme1390I CCNGG 2 cut(s) 131, 773
Bme18I GGWCC 2 cut(s) 99, 148
BmgT120I GGNCC 2 cut(s) 99, 148
BmiI GGNNCC 4 cut(s) 100, 670, 687, 915
BmrFI CCNGG 2 cut(s) 131, 773
BmsI GCATC 4 cut(s) 183, 749, 883, 919
BpiI GAAGAC 3 cut(s) 152, 412, 779
BpmI CTGGAG 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 5 cut(s) 129, 168, 216, 381, 771
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 289, 997
Bse118I RCCGGY 1 cut(s) 821
Bse3DI GCAATG 2 cut(s) 203, 208
BseBI CCWGG 2 cut(s) 131, 773
BseDI CCNNGG 5 cut(s) 129, 168, 216, 381, 771
BseGI GGATG 3 cut(s) 10, 453, 980
BseLI CCNNNNNNNGG 3 cut(s) 222, 289, 997
BseMI GCAATG 2 cut(s) 203, 208
BseMII CTCAG 4 cut(s) 180, 387, 735, 834
BseSI GKGCMC 1 cut(s) 770
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 1 cut(s) 709
BsgI GTGCAG 1 cut(s) 512
Bsh1236I CGCG 1 cut(s) 435
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 120, 237, 821
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 3 cut(s) 339, 637, 770
BsiSI CCGG 2 cut(s) 141, 822
BslFI GGGAC 2 cut(s) 112, 1101
BslI CCNNNNNNNGG 3 cut(s) 222, 289, 997
BsmAI GTCTC 3 cut(s) 339, 748, 830
BsmFI GGGAC 2 cut(s) 112, 1101
BsnI GGCC 3 cut(s) 120, 237, 821
Bso31I GGTCTC 1 cut(s) 830
Bsp1286I GDGCHC 3 cut(s) 339, 637, 770
Bsp143I GATC 3 cut(s) 430, 541, 913
Bsp19I CCATGG 1 cut(s) 381
Bsp68I TCGCGA 1 cut(s) 435
BspACI CCGC 2 cut(s) 623, 911
BspANI GGCC 3 cut(s) 120, 237, 821
BspCNI CTCAG 4 cut(s) 181, 386, 736, 835
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 4 cut(s) 100, 670, 687, 915
BspPI GGATC 3 cut(s) 549, 908, 921
BspQI GCTCTTC 2 cut(s) 730, 1005
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 2 cut(s) 203, 208
BsrFI RCCGGY 1 cut(s) 821
BssAI RCCGGY 1 cut(s) 821
BssECI CCNNGG 5 cut(s) 129, 168, 216, 381, 771
BssMI GATC 3 cut(s) 430, 541, 913
BssT1I CCWWGG 2 cut(s) 216, 381
Bst2UI CCWGG 2 cut(s) 131, 773
Bst4CI ACNGT 5 cut(s) 110, 269, 324, 334, 490
Bst6I CTCTTC 2 cut(s) 730, 1005
BstC8I GCNNGC 2 cut(s) 186, 1017
BstDEI CTNAG 5 cut(s) 189, 373, 744, 843, 1060
BstDSI CCRYGG 1 cut(s) 381
BstF5I GGATG 3 cut(s) 10, 453, 980
BstFNI CGCG 1 cut(s) 435
BstKTI GATC 3 cut(s) 433, 544, 916
BstMAI GTCTC 3 cut(s) 339, 748, 830
BstMBI GATC 3 cut(s) 430, 541, 913
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 2 cut(s) 131, 773
BstSCI CCNGG 2 cut(s) 129, 771
BstSFI CTRYAG 2 cut(s) 458, 519
BstSLI GKGCMC 1 cut(s) 770
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 709
BstV2I GAAGAC 3 cut(s) 152, 412, 779
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 3 cut(s) 120, 237, 821
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 3 cut(s) 10, 453, 980
BtsI GCAGTG 3 cut(s) 186, 939, 1044
BtsIMutI CAGTG 6 cut(s) 186, 248, 468, 539, 939, 1044
BtuMI TCGCGA 1 cut(s) 435
Cac8I GCNNGC 2 cut(s) 186, 1017
Cfr10I RCCGGY 1 cut(s) 821
Cfr13I GGNCC 2 cut(s) 99, 148
CpoI CGGWCCG 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CspI CGGWCCG 1 cut(s) 148
CviAII CATG 4 cut(s) 14, 382, 605, 640
DdeI CTNAG 5 cut(s) 189, 373, 744, 843, 1060
DpnI GATC 3 cut(s) 432, 543, 915
DpnII GATC 3 cut(s) 430, 541, 913
EaeI YGGCCR 1 cut(s) 235
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 2 cut(s) 730, 1005
EarI CTCTTC 2 cut(s) 730, 1005
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 216, 381
Eco31I GGTCTC 1 cut(s) 830
Eco32I GATATC 1 cut(s) 849
Eco47I GGWCC 2 cut(s) 99, 148
Eco52I CGGCCG 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 99
EcoRII CCWGG 2 cut(s) 129, 771
EcoRV GATATC 1 cut(s) 849
EcoT14I CCWWGG 2 cut(s) 216, 381
ErhI CCWWGG 2 cut(s) 216, 381
FaeI CATG 4 cut(s) 17, 385, 608, 643
FaqI GGGAC 2 cut(s) 112, 1101
FatI CATG 4 cut(s) 13, 381, 604, 639
FauI CCCGC 1 cut(s) 616
Fnu4HI GCNGC 1 cut(s) 723
FokI GGATG 3 cut(s) 17, 440, 967
Fsp4HI GCNGC 1 cut(s) 723
FspBI CTAG 2 cut(s) 791, 1022
GluI GCNGC 1 cut(s) 723
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 3 cut(s) 120, 237, 821
HapII CCGG 2 cut(s) 141, 822
Hin1II CATG 4 cut(s) 17, 385, 608, 643
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 7 cut(s) 88, 212, 293, 408, 473, 1072, 1089
HpaI GTTAAC 1 cut(s) 138
HpaII CCGG 2 cut(s) 141, 822
HphI GGTGA 3 cut(s) 793, 880, 1013
Hpy166II GTNNAC 2 cut(s) 138, 768
Hpy188I TCNGA 9 cut(s) 83, 169, 360, 417, 480, 685, 745, 815, 997
Hpy188III TCNNGA 4 cut(s) 97, 434, 917, 1107
Hpy8I GTNNAC 2 cut(s) 138, 768
HpyAV CCTTC 3 cut(s) 776, 964, 1062
HpyCH4III ACNGT 5 cut(s) 110, 269, 324, 334, 490
HpyCH4IV ACGT 1 cut(s) 876
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 5 cut(s) 189, 373, 744, 843, 1060
HpySE526I ACGT 1 cut(s) 876
Hsp92II CATG 4 cut(s) 17, 385, 608, 643
KspAI GTTAAC 1 cut(s) 138
Kzo9I GATC 3 cut(s) 430, 541, 913
LguI GCTCTTC 2 cut(s) 730, 1005
LmnI GCTCC 2 cut(s) 510, 1056
Lsp1109I GCAGC 1 cut(s) 709
LweI GCATC 4 cut(s) 183, 749, 883, 919
MaeI CTAG 2 cut(s) 791, 1022
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 9 cut(s) 16, 75, 388, 484, 799, 832, 886, 956, 1001
MalI GATC 3 cut(s) 432, 543, 915
MboI GATC 3 cut(s) 430, 541, 913
MboII GAAGA 5 cut(s) 152, 417, 747, 779, 1022
MfeI CAATTG 3 cut(s) 285, 312, 897
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 3 cut(s) 339, 637, 770
MlyI GAGTC 4 cut(s) 221, 287, 402, 1083
MmeI TCCRAC 1 cut(s) 129
MroXI GAANNNNTTC 2 cut(s) 244, 786
MseI TTAA 3 cut(s) 41, 137, 615
MslI CAYNNNNRTG 1 cut(s) 663
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 2 cut(s) 141, 822
MspR9I CCNGG 2 cut(s) 131, 773
MunI CAATTG 3 cut(s) 285, 312, 897
MvaI CCWGG 2 cut(s) 131, 773
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 185
NcoI CCATGG 1 cut(s) 381
NdeII GATC 3 cut(s) 430, 541, 913
NlaIII CATG 4 cut(s) 17, 385, 608, 643
NlaIV GGNNCC 4 cut(s) 100, 670, 687, 915
NmuCI GTSAC 7 cut(s) 75, 388, 799, 832, 886, 956, 1001
NruI TCGCGA 1 cut(s) 435
PciSI GCTCTTC 2 cut(s) 730, 1005
PdmI GAANNNNTTC 2 cut(s) 244, 786
PfeI GAWTC 3 cut(s) 88, 473, 1072
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 1 cut(s) 724
PleI GAGTC 4 cut(s) 220, 287, 402, 1083
PpsI GAGTC 4 cut(s) 220, 287, 402, 1083
PpuMI RGGWCCY 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 876
Psp5II RGGWCCY 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 129, 771
PspGI CCWGG 2 cut(s) 129, 771
PspN4I GGNNCC 4 cut(s) 100, 670, 687, 915
PspPI GGNCC 2 cut(s) 99, 148
PspPPI RGGWCCY 1 cut(s) 99
PsuI RGATCY 1 cut(s) 913
RruI TCGCGA 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 663
Rsr2I CGGWCCG 1 cut(s) 148
RsrII CGGWCCG 1 cut(s) 148
SapI GCTCTTC 2 cut(s) 730, 1005
SaqAI TTAA 3 cut(s) 41, 137, 615
SatI GCNGC 1 cut(s) 723
Sau3AI GATC 3 cut(s) 430, 541, 913
Sau96I GGNCC 2 cut(s) 99, 148
SchI GAGTC 4 cut(s) 221, 287, 402, 1083
ScrFI CCNGG 2 cut(s) 131, 773
SduI GDGCHC 3 cut(s) 339, 637, 770
SfaNI GCATC 4 cut(s) 183, 749, 883, 919
SfcI CTRYAG 2 cut(s) 458, 519
SinI GGWCC 2 cut(s) 99, 148
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
SsiI CCGC 2 cut(s) 623, 911
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 791, 1022
StyD4I CCNGG 2 cut(s) 129, 771
StyI CCWWGG 2 cut(s) 216, 381
TaaI ACNGT 5 cut(s) 110, 269, 324, 334, 490
TaiI ACGT 1 cut(s) 879
TaqII GACCGA 1 cut(s) 165
TfiI GAWTC 3 cut(s) 88, 473, 1072
Tru1I TTAA 3 cut(s) 41, 137, 615
Tru9I TTAA 3 cut(s) 41, 137, 615
TscAI CASTG 6 cut(s) 186, 255, 475, 539, 939, 1044
TseFI GTSAC 7 cut(s) 75, 388, 799, 832, 886, 956, 1001
TseI GCWGC 1 cut(s) 722
Tsp45I GTSAC 7 cut(s) 75, 388, 799, 832, 886, 956, 1001
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 3 cut(s) 59, 161, 973
TspRI CASTG 6 cut(s) 186, 255, 475, 539, 939, 1044
Van91I CCANNNNNTGG 1 cut(s) 222
VneI GTGCAC 1 cut(s) 766
VpaK11BI GGWCC 2 cut(s) 99, 148
XapI RAATTY 1 cut(s) 644
XmnI GAANNNNTTC 2 cut(s) 244, 786
XspI CTAG 2 cut(s) 791, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.