Rh7DG192800

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012060
Physical Location & Seq
Forward (+)
18034675 .. 18036849
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG192800.1

Sequence Viewer

Length: 1326 bp
ATGGAGTGGACTGCTTGTCTTGATGAATATGAGAAACTCGTCTTCCGAATGAACACCCCTAGGGTCGTGATTGACAATGCCGTCTGCTCCACTGCGACTTTAGTCAAGGTCGACAGTGCTAGAAGGCACGGAATTCTTCTAGAGGCTGTTCAGGTCCTCACAGACCTAAATCTCACCATTAAAAAAGCTTATATTTCTTCTGACGGACAATGGTTCATGGATGTTTTCCATGTTACTGACCAAAATGGGAACAAATTAACGGACGAGAGCGTTATCAATTACATTGAGCAGTCACTCGGAACGATTCACTTCGGGACCTCCAACTGTTCTAACGGCCTAACAGCCCTGGAGTTAACCGGAACGGACCGAGTTGGTCTTCTTTCCGAGGTCTTTGCAGTGCTGGCTGAGATGCAATGCAATGTGGTTGAGTCCAAGGTATGGACACACAACGGCCGAATTGCTTCACTGATTTATGTCAAGGACTGTGGTTCAGGTTGCCCAATTGAGGACTCCCAGAAAATTGATACAATTGAAGCGCGGTTGAGGACTGTGCTCAAGGGAGACAATGACATTCGGAGTGCGAAAACCTCAGTTTCCATGGCTGTCACGCACACCGAAAGAAGACTCCATCAGATGATGTTTGCAGATCGCGATTATGAAAGGAAGCCCATCCTACAGCATTGCACTGATTCTCCGATTGTAACGGTGCAGAATTGGGCCGAAAGGAGCTATTCTGTAGTGAATATTCAGTGCAAGGATCGGGCAAAGCTTTTGTTCGATGTTGTTTGCACAATGACAGATATGGACTATGTGGTTTTCCATGCCACGATTAAAACAGCGGGAAATAGTGCACACATGGAATTTTACATTAGGCATACTGATGGAACCCCAATTAGTTCTGAACCCGAACGACATCGTGTAATTCAATGCTTACAAGCTGCCATTGAAAGAAGAGCATCTGAGGGTGTGAGACTTGAATTGTGCACCAAGGATAGAGAAGGTCTTCTAGCAAATGTCACCAGAACATTCCGAGAGGCCGGTCTCAATGTGACAAGGGCCGAGATATCCACCTCAACAGACAAGGCTCTAAACGTTTTCTATGTCACCGATGCAATTGGAAACCCTGCGGATCCAGAAACCATTGATGCAGTGAGGCAGAAAGTAGGGTTGAGTGACTTGAAAGTGAAGGAATTGCCATCCGTTTGCCATTCAGAGGGTGACAGAGAAGAGCAAGCACTAGGGGTTGGTGGGGCAGTGCTGTTATCACTTGGGAGCTTAGTGAGAAGGAATCTATACAATTTGGGACTCATCAGGTCTTTTTCTTGA

Protein Analysis

441

Amino Acids

49.1

Weight (kDa)

5.79

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 115 - 161 2.8e-06 ACT domain
ACT_6 PF13740 117 - 153 4.5e-06 ACT domain
ACT_ACR9_3rd PF24931 234 - 316 1e-08 ACR9-like, ACT-like domain
ACT PF01842 324 - 385 7.3e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 438
AccI GTMKAC 1 cut(s) 111
AccII CGCG 2 cut(s) 538, 651
AciI CCGC 3 cut(s) 538, 839, 1127
AclI AACGTT 1 cut(s) 1092
AclWI GGATC 3 cut(s) 765, 1124, 1137
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 2 cut(s) 132, 860
AfiI CCNNNNNNNGG 3 cut(s) 438, 505, 1213
AgsI TTSAA 5 cut(s) 533, 926, 947, 977, 1180
AhdI GACNNNNNGTC 1 cut(s) 15
AjnI CCWGG 1 cut(s) 345
AluBI AGCT 5 cut(s) 188, 729, 769, 938, 1275
AluI AGCT 5 cut(s) 188, 729, 769, 938, 1275
Alw21I GWGCWC 3 cut(s) 555, 853, 986
Alw26I GTCTC 3 cut(s) 555, 964, 1046
Alw44I GTGCAC 2 cut(s) 849, 982
AlwI GGATC 3 cut(s) 765, 1124, 1137
AoxI GGCC 5 cut(s) 334, 451, 717, 1035, 1056
ApaLI GTGCAC 2 cut(s) 849, 982
ApeKI GCWGC 1 cut(s) 938
ApoI RAATTY 2 cut(s) 132, 860
Asp700I GAANNNNTTC 2 cut(s) 460, 1002
AspA2I CCTAGG 1 cut(s) 59
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 5 cut(s) 154, 315, 364, 717, 1056
AsuHPI GGTGA 4 cut(s) 166, 1009, 1096, 1229
AvaII GGWCC 3 cut(s) 154, 315, 364
AvrII CCTAGG 1 cut(s) 59
BaeGI GKGCMC 2 cut(s) 853, 986
BamHI GGATCC 1 cut(s) 1129
BbsI GAAGAC 4 cut(s) 34, 368, 628, 995
Bbv12I GWGCWC 3 cut(s) 555, 853, 986
BbvI GCAGC 1 cut(s) 925
BccI CCATC 4 cut(s) 636, 677, 875, 1204
BceAI ACGGC 3 cut(s) 65, 349, 466
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 555, 964, 1046
BfaI CTAG 5 cut(s) 60, 120, 140, 1007, 1238
BfmI CTRYAG 2 cut(s) 674, 735
BisI GCNGC 1 cut(s) 939
BlnI CCTAGG 1 cut(s) 59
BlsI GCNGC 1 cut(s) 940
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 3 cut(s) 154, 315, 364
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 5 cut(s) 154, 315, 364, 717, 1056
BmiI GGNNCC 3 cut(s) 316, 886, 1131
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 4 cut(s) 399, 965, 1099, 1135
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 4 cut(s) 34, 368, 628, 995
BpmI CTGGAG 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 1046
BsaJI CCNNGG 6 cut(s) 59, 345, 384, 432, 597, 987
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 676, 706
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 505, 1213
Bse118I RCCGGY 1 cut(s) 1037
Bse3DI GCAATG 3 cut(s) 419, 424, 679
BseBI CCWGG 1 cut(s) 347
BseDI CCNNGG 6 cut(s) 59, 345, 384, 432, 597, 987
BseGI GGATG 3 cut(s) 226, 669, 1196
BseLI CCNNNNNNNGG 3 cut(s) 438, 505, 1213
BseMI GCAATG 3 cut(s) 419, 424, 679
BseMII CTCAG 3 cut(s) 396, 603, 951
BseSI GKGCMC 2 cut(s) 853, 986
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 1 cut(s) 925
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 2 cut(s) 538, 651
Bsh1285I CGRYCG 1 cut(s) 454
BshFI GGCC 5 cut(s) 336, 453, 719, 1037, 1058
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 3 cut(s) 555, 853, 986
BsiSI CCGG 2 cut(s) 357, 1038
BslFI GGGAC 2 cut(s) 328, 1317
BslI CCNNNNNNNGG 3 cut(s) 438, 505, 1213
BsmAI GTCTC 3 cut(s) 555, 964, 1046
BsmFI GGGAC 2 cut(s) 328, 1317
BsnI GGCC 5 cut(s) 336, 453, 719, 1037, 1058
Bso31I GGTCTC 1 cut(s) 1046
Bsp1286I GDGCHC 3 cut(s) 555, 853, 986
Bsp143I GATC 3 cut(s) 646, 757, 1129
Bsp19I CCATGG 1 cut(s) 597
Bsp68I TCGCGA 1 cut(s) 651
BspACI CCGC 3 cut(s) 538, 839, 1127
BspANI GGCC 5 cut(s) 336, 453, 719, 1037, 1058
BspCNI CTCAG 3 cut(s) 397, 602, 952
BspFNI CGCG 2 cut(s) 538, 651
BspLI GGNNCC 3 cut(s) 316, 886, 1131
BspPI GGATC 3 cut(s) 765, 1124, 1137
BspQI GCTCTTC 2 cut(s) 946, 1221
BspTNI GGTCTC 1 cut(s) 1046
BsrDI GCAATG 3 cut(s) 419, 424, 679
BsrFI RCCGGY 1 cut(s) 1037
BssAI RCCGGY 1 cut(s) 1037
BssECI CCNNGG 6 cut(s) 59, 345, 384, 432, 597, 987
BssMI GATC 3 cut(s) 646, 757, 1129
BssT1I CCWWGG 4 cut(s) 59, 432, 597, 987
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 5 cut(s) 116, 326, 485, 550, 706
Bst6I CTCTTC 2 cut(s) 946, 1221
BstC8I GCNNGC 2 cut(s) 402, 1233
BstDEI CTNAG 4 cut(s) 405, 589, 960, 1276
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 3 cut(s) 226, 669, 1196
BstFNI CGCG 2 cut(s) 538, 651
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 3 cut(s) 649, 760, 1132
BstMAI GTCTC 3 cut(s) 555, 964, 1046
BstMBI GATC 3 cut(s) 646, 757, 1129
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 1 cut(s) 347
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 345
BstSFI CTRYAG 2 cut(s) 674, 735
BstSLI GKGCMC 2 cut(s) 853, 986
BstUI CGCG 2 cut(s) 538, 651
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 4 cut(s) 34, 368, 628, 995
BstX2I RGATCY 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 1129
BstZI CGGCCG 1 cut(s) 451
BsuRI GGCC 5 cut(s) 336, 453, 719, 1037, 1058
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 3 cut(s) 226, 669, 1196
BtsI GCAGTG 4 cut(s) 90, 402, 1155, 1260
BtsIMutI CAGTG 8 cut(s) 90, 121, 402, 464, 684, 755, 1155, 1260
BtuMI TCGCGA 1 cut(s) 651
Cac8I GCNNGC 2 cut(s) 402, 1233
CfoI GCGC 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 1037
Cfr13I GGNCC 5 cut(s) 154, 315, 364, 717, 1056
CpoI CGGWCCG 1 cut(s) 364
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CspI CGGWCCG 1 cut(s) 364
CviAII CATG 5 cut(s) 217, 230, 598, 821, 856
DdeI CTNAG 4 cut(s) 405, 589, 960, 1276
DpnI GATC 3 cut(s) 648, 759, 1131
DpnII GATC 3 cut(s) 646, 757, 1129
DrdI GACNNNNNNGTC 1 cut(s) 80
DriI GACNNNNNGTC 1 cut(s) 15
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 451
EagI CGGCCG 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 946, 1221
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 2 cut(s) 946, 1221
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 4 cut(s) 59, 432, 597, 987
Eco31I GGTCTC 1 cut(s) 1046
Eco32I GATATC 1 cut(s) 1065
Eco47I GGWCC 3 cut(s) 154, 315, 364
Eco52I CGGCCG 1 cut(s) 451
EcoO109I RGGNCCY 2 cut(s) 154, 315
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 345
EcoRV GATATC 1 cut(s) 1065
EcoT14I CCWWGG 4 cut(s) 59, 432, 597, 987
ErhI CCWWGG 4 cut(s) 59, 432, 597, 987
FaeI CATG 5 cut(s) 220, 233, 601, 824, 859
FaqI GGGAC 2 cut(s) 328, 1317
FatI CATG 5 cut(s) 216, 229, 597, 820, 855
FauI CCCGC 1 cut(s) 832
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 939
FokI GGATG 3 cut(s) 233, 656, 1183
Fsp4HI GCNGC 1 cut(s) 939
FspBI CTAG 5 cut(s) 60, 120, 140, 1007, 1238
GlaI GCGC 1 cut(s) 537
GluI GCNGC 1 cut(s) 939
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 5 cut(s) 336, 453, 719, 1037, 1058
HapII CCGG 2 cut(s) 357, 1038
HhaI GCGC 1 cut(s) 538
Hin1II CATG 5 cut(s) 220, 233, 601, 824, 859
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HincII GTYRAC 2 cut(s) 112, 354
HindII GTYRAC 2 cut(s) 112, 354
HindIII AAGCTT 2 cut(s) 186, 767
HinfI GANTC 7 cut(s) 304, 428, 509, 624, 689, 1288, 1305
HpaI GTTAAC 1 cut(s) 354
HpaII CCGG 2 cut(s) 357, 1038
HphI GGTGA 4 cut(s) 166, 1009, 1096, 1229
Hpy166II GTNNAC 5 cut(s) 9, 112, 354, 851, 984
Hpy188III TCNNGA 7 cut(s) 20, 67, 140, 313, 650, 1133, 1323
Hpy8I GTNNAC 5 cut(s) 9, 112, 354, 851, 984
HpyAV CCTTC 4 cut(s) 117, 992, 1180, 1278
HpyCH4III ACNGT 5 cut(s) 116, 326, 485, 550, 706
HpyCH4IV ACGT 1 cut(s) 1092
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 4 cut(s) 405, 589, 960, 1276
HpySE526I ACGT 1 cut(s) 1092
Hsp92II CATG 5 cut(s) 220, 233, 601, 824, 859
HspAI GCGC 1 cut(s) 536
KspAI GTTAAC 1 cut(s) 354
Kzo9I GATC 3 cut(s) 646, 757, 1129
LguI GCTCTTC 2 cut(s) 946, 1221
LmnI GCTCC 3 cut(s) 92, 726, 1272
Lsp1109I GCAGC 1 cut(s) 925
LweI GCATC 4 cut(s) 399, 965, 1099, 1135
MaeI CTAG 5 cut(s) 60, 120, 140, 1007, 1238
MaeII ACGT 1 cut(s) 1092
MaeIII GTNAC 9 cut(s) 232, 291, 604, 700, 1015, 1048, 1102, 1172, 1217
MalI GATC 3 cut(s) 648, 759, 1131
MboI GATC 3 cut(s) 646, 757, 1129
MboII GAAGA 8 cut(s) 34, 128, 189, 368, 633, 963, 995, 1238
MfeI CAATTG 3 cut(s) 501, 528, 1113
MflI RGATCY 1 cut(s) 1129
MhlI GDGCHC 3 cut(s) 555, 853, 986
MlyI GAGTC 4 cut(s) 437, 503, 618, 1299
MmeI TCCRAC 1 cut(s) 345
MroXI GAANNNNTTC 2 cut(s) 460, 1002
MseI TTAA 4 cut(s) 180, 257, 353, 831
MslI CAYNNNNRTG 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 2 cut(s) 357, 1038
MspR9I CCNGG 1 cut(s) 347
MunI CAATTG 3 cut(s) 501, 528, 1113
MvaI CCWGG 1 cut(s) 347
MvnI CGCG 2 cut(s) 538, 651
MwoI GCNNNNNNNGC 1 cut(s) 401
NcoI CCATGG 1 cut(s) 597
NdeII GATC 3 cut(s) 646, 757, 1129
NlaIII CATG 5 cut(s) 220, 233, 601, 824, 859
NlaIV GGNNCC 3 cut(s) 316, 886, 1131
NmeAIII GCCGAG 1 cut(s) 1084
NmuCI GTSAC 7 cut(s) 291, 604, 1015, 1048, 1102, 1172, 1217
NruI TCGCGA 1 cut(s) 651
PciSI GCTCTTC 2 cut(s) 946, 1221
PdmI GAANNNNTTC 2 cut(s) 460, 1002
PfeI GAWTC 3 cut(s) 304, 689, 1288
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 1 cut(s) 940
PleI GAGTC 4 cut(s) 436, 503, 618, 1299
PpsI GAGTC 4 cut(s) 436, 503, 618, 1299
PpuMI RGGWCCY 2 cut(s) 154, 315
PshAI GACNNNNGTC 1 cut(s) 101
Psp1406I AACGTT 1 cut(s) 1092
Psp5II RGGWCCY 2 cut(s) 154, 315
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 316, 886, 1131
PspPI GGNCC 5 cut(s) 154, 315, 364, 717, 1056
PspPPI RGGWCCY 2 cut(s) 154, 315
PsuI RGATCY 1 cut(s) 1129
RruI TCGCGA 1 cut(s) 651
RseI CAYNNNNRTG 1 cut(s) 879
Rsr2I CGGWCCG 1 cut(s) 364
RsrII CGGWCCG 1 cut(s) 364
SalI GTCGAC 1 cut(s) 110
SapI GCTCTTC 2 cut(s) 946, 1221
SaqAI TTAA 4 cut(s) 180, 257, 353, 831
SatI GCNGC 1 cut(s) 939
Sau3AI GATC 3 cut(s) 646, 757, 1129
Sau96I GGNCC 5 cut(s) 154, 315, 364, 717, 1056
SchI GAGTC 4 cut(s) 437, 503, 618, 1299
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 3 cut(s) 555, 853, 986
SfaNI GCATC 4 cut(s) 399, 965, 1099, 1135
SfcI CTRYAG 2 cut(s) 674, 735
SinI GGWCC 3 cut(s) 154, 315, 364
SmiMI CAYNNNNRTG 1 cut(s) 879
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SsiI CCGC 3 cut(s) 538, 839, 1127
SspI AATATT 1 cut(s) 745
SspMI CTAG 5 cut(s) 60, 120, 140, 1007, 1238
StyD4I CCNGG 1 cut(s) 345
StyI CCWWGG 4 cut(s) 59, 432, 597, 987
TaaI ACNGT 5 cut(s) 116, 326, 485, 550, 706
TaiI ACGT 1 cut(s) 1095
TaqI TCGA 2 cut(s) 111, 777
TaqII GACCGA 1 cut(s) 381
TfiI GAWTC 3 cut(s) 304, 689, 1288
Tru1I TTAA 4 cut(s) 180, 257, 353, 831
Tru9I TTAA 4 cut(s) 180, 257, 353, 831
TscAI CASTG 8 cut(s) 97, 121, 402, 471, 691, 755, 1155, 1260
TseFI GTSAC 7 cut(s) 291, 604, 1015, 1048, 1102, 1172, 1217
TseI GCWGC 1 cut(s) 938
Tsp45I GTSAC 7 cut(s) 291, 604, 1015, 1048, 1102, 1172, 1217
TspDTI ATGAA 4 cut(s) 39, 65, 205, 672
TspGWI ACGGA 5 cut(s) 144, 219, 275, 377, 1189
TspRI CASTG 8 cut(s) 97, 121, 402, 471, 691, 755, 1155, 1260
Van91I CCANNNNNTGG 1 cut(s) 438
VneI GTGCAC 2 cut(s) 849, 982
VpaK11BI GGWCC 3 cut(s) 154, 315, 364
XapI RAATTY 2 cut(s) 132, 860
XbaI TCTAGA 1 cut(s) 139
XmaJI CCTAGG 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 2 cut(s) 460, 1002
XspI CTAG 5 cut(s) 60, 120, 140, 1007, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.