Prupe.5G081600_v2.0.a1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012060
Physical Location & Seq
Reverse (-)
9492516 .. 9496287
3772 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G081600.1

Sequence Viewer

Length: 1326 bp
ATGGAGTGGACTGCTTGTCTCGACGAATATGAGAAACTCGTCTTCCGTATGAACACTCCCAGGGTCGTGATCGATAATGCCGTCTGCTCCACTGCGACTTTAGTCAAGGTTGACAGTGCTAGAAGGCATGGGATTCTTCTCGAGGCTGTTCAGGTCCTCACGGATCTTAACCTCTCCATTAAAAAAGCGTACATTTCTTCTGATGGACGGTGGTTCATGGATGTCTTTCATGTCACTGATCAAAACGGGGATAAATTAACGGATGAGAGCGTCATTCATTACATTGAACAGTCACTTGGAACAATTCACTTTGGGACATCCAACTGCAGCAACGGTTTAACAGCACTTGAGCTCACTGGAACTGACCGGCTTGGCCTTCTCTCTGAGGTCTTTGCAGTGCTAGCTGACCTGCAATGCAATGTGGTTGAGTCCAAGGTGTGGACGCACAATGGCCGGATCGCCTCCCTAATTTACGTCAAGGACTGTGATTCTGGTTGCCCAATTGAAGACTCACAGAAAATTGACCGAATTGAAGCACGGTTAAGGAATGTTCTCAAGGGAGACAACGATATTCGGAGCGCGAAAACCTCTGTTTCCATGGCAGTTACGCACACCGAAAGAAGGCTGCATCAAATGATGTTTGCAGATCGTGATTATGACAGGAAGCCCATTTTGCAGCACAGCACCGACTCACCGGTGATCACAGTGCAGAATTGGGCTGAAAGGGGTTATTCCATAGTAAATATCCAGTGCAAGGATAGAGCAAAGCTATTGTTTGATGTTGTTTGCACGTTGACAGACATGGACTATGTGGTTTTTCACGCCACTATTAAAACAGCAGGGGATAGAGGACACATGGAATTTTACATTAGGCACACTGATGGAACCCCAATTAGTTCTGAACCCGAACGACAGCGCGTAATCCAATGCTTACAAGCTGCCATTCAAAGAAGAGCTTCTGAGGGTGTGAGGCTAGAACTATGCACCGAGGATAGACCAGGTCTTCTGGCATATGTTACAAGAACGTTCCGAGAGAACGGTCTAAACGTGACAAGGGCTGAGATTTCCACAACAAAGGACAATGCCCTGAACGTTTTCTATGTGACTGATGCAATGGGAAACCCTGCAGATCCCAACACAATTGAATCAGTGAGGCAAAAAATAGGGTTGAGTGACTTAAAAGTGAAGGAACTGCCATTGATTTATCATGCAAAGGGGGAAAGGGAGGAGCAAGCAGTTGGTGTTGGTGGGGCAGTCTTGTTATCACTTGGGAGCCTAGTGAGAAGGAATCTGTACAATTTGGGTCTCATCAGATCTCATTCTTGA

Protein Analysis

442

Amino Acids

49.43

Weight (kDa)

6.14

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 2 cut(s) 581, 918
AclI AACGTT 2 cut(s) 1025, 1092
AclWI GGATC 3 cut(s) 171, 464, 1124
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 1 cut(s) 860
AfaI GTAC 2 cut(s) 191, 1295
AfiI CCNNNNNNNGG 3 cut(s) 438, 621, 754
AgeI ACCGGT 1 cut(s) 694
AgsI TTSAA 5 cut(s) 287, 506, 533, 947, 1145
AhdI GACNNNNNGTC 1 cut(s) 15
AjnI CCWGG 2 cut(s) 59, 997
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 5 cut(s) 352, 404, 769, 938, 956
AluI AGCT 5 cut(s) 352, 404, 769, 938, 956
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 3 cut(s) 23, 555, 1310
AlwI GGATC 3 cut(s) 171, 464, 1124
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 2 cut(s) 373, 451
ApeKI GCWGC 4 cut(s) 327, 625, 676, 938
ApoI RAATTY 1 cut(s) 860
AsiGI ACCGGT 1 cut(s) 694
Asp700I GAANNNNTTC 2 cut(s) 955, 1094
AspLEI GCGC 2 cut(s) 581, 918
AspS9I GGNCC 1 cut(s) 154
AsuHPI GGTGA 2 cut(s) 684, 709
AsuNHI GCTAGC 1 cut(s) 400
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 354
BbsI GAAGAC 3 cut(s) 34, 513, 995
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 4 cut(s) 339, 612, 688, 925
BccI CCATC 2 cut(s) 197, 875
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 2 cut(s) 61, 999
BclI TGATCA 2 cut(s) 238, 699
BcoDI GTCTC 3 cut(s) 23, 555, 1310
BfaI CTAG 4 cut(s) 120, 401, 974, 1277
BfmI CTRYAG 2 cut(s) 325, 1125
BfuAI ACCTGC 1 cut(s) 417
BglII AGATCT 1 cut(s) 1313
BisI GCNGC 4 cut(s) 328, 626, 677, 939
BlsI GCNGC 4 cut(s) 329, 627, 678, 940
Bme1390I CCNGG 2 cut(s) 61, 999
Bme18I GGWCC 1 cut(s) 154
BmeRI GACNNNNNGTC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 886, 1274
BmrFI CCNGG 2 cut(s) 61, 999
BmsI GCATC 2 cut(s) 637, 1099
BmtI GCTAGC 1 cut(s) 404
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 3 cut(s) 34, 513, 995
BpuEI CTTGAG 2 cut(s) 368, 539
Bsa29I ATCGAT 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1310
BsaJI CCNNGG 5 cut(s) 59, 60, 432, 597, 987
BsaWI WCCGGW 1 cut(s) 694
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 621, 754
Bse118I RCCGGY 2 cut(s) 366, 694
Bse1I ACTGG 2 cut(s) 361, 748
Bse3DI GCAATG 3 cut(s) 419, 424, 1119
BseBI CCWGG 2 cut(s) 61, 999
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 5 cut(s) 59, 60, 432, 597, 987
BseGI GGATG 3 cut(s) 226, 268, 317
BseLI CCNNNNNNNGG 3 cut(s) 438, 621, 754
BseMI GCAATG 3 cut(s) 419, 424, 1119
BseMII CTCAG 3 cut(s) 375, 951, 1050
BseNI ACTGG 2 cut(s) 361, 748
BseRI GAGGAG 1 cut(s) 1241
BseXI GCAGC 4 cut(s) 339, 612, 688, 925
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 2 cut(s) 581, 918
BshFI GGCC 2 cut(s) 375, 453
BshTI ACCGGT 1 cut(s) 694
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 3 cut(s) 367, 454, 695
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 3 cut(s) 438, 621, 754
BsmAI GTCTC 3 cut(s) 23, 555, 1310
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 2 cut(s) 375, 453
Bso31I GGTCTC 1 cut(s) 1310
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 354
Bsp1407I TGTACA 1 cut(s) 1293
Bsp143I GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
Bsp19I CCATGG 1 cut(s) 597
BspANI GGCC 2 cut(s) 375, 453
BspCNI CTCAG 3 cut(s) 376, 952, 1051
BspDI ATCGAT 1 cut(s) 72
BspFNI CGCG 2 cut(s) 581, 918
BspLI GGNNCC 2 cut(s) 886, 1274
BspMAI CTGCAG 2 cut(s) 329, 1129
BspMI ACCTGC 1 cut(s) 417
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 3 cut(s) 171, 464, 1124
BspQI GCTCTTC 1 cut(s) 946
BspTNI GGTCTC 1 cut(s) 1310
BsrDI GCAATG 3 cut(s) 419, 424, 1119
BsrFI RCCGGY 2 cut(s) 366, 694
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 2 cut(s) 361, 748
BssAI RCCGGY 2 cut(s) 366, 694
BssECI CCNNGG 5 cut(s) 59, 60, 432, 597, 987
BssMI GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
BssT1I CCWWGG 2 cut(s) 432, 597
Bst2UI CCWGG 2 cut(s) 61, 999
Bst4CI ACNGT 8 cut(s) 116, 210, 291, 335, 485, 540, 706, 1040
Bst6I CTCTTC 1 cut(s) 946
BstAUI TGTACA 1 cut(s) 1293
BstC8I GCNNGC 2 cut(s) 402, 1233
BstDEI CTNAG 3 cut(s) 384, 960, 1059
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 3 cut(s) 226, 268, 317
BstFNI CGCG 2 cut(s) 581, 918
BstHHI GCGC 2 cut(s) 581, 918
BstKTI GATC 8 cut(s) 72, 166, 241, 459, 649, 702, 1132, 1316
BstMAI GTCTC 3 cut(s) 23, 555, 1310
BstMBI GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
BstMWI GCNNNNNNNGC 2 cut(s) 401, 673
BstNI CCWGG 2 cut(s) 61, 999
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 59, 997
BstSFI CTRYAG 2 cut(s) 325, 1125
BstUI CGCG 2 cut(s) 581, 918
BstV1I GCAGC 4 cut(s) 339, 612, 688, 925
BstV2I GAAGAC 3 cut(s) 34, 513, 995
BstX2I RGATCY 3 cut(s) 163, 1129, 1313
BstYI RGATCY 3 cut(s) 163, 1129, 1313
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 2 cut(s) 375, 453
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 3 cut(s) 226, 268, 317
BtsI GCAGTG 2 cut(s) 90, 402
BtsIMutI CAGTG 9 cut(s) 90, 121, 234, 354, 402, 711, 755, 876, 1155
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 402, 1233
CfoI GCGC 2 cut(s) 581, 918
Cfr10I RCCGGY 2 cut(s) 366, 694
Cfr13I GGNCC 1 cut(s) 154
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 2 cut(s) 259, 451
CsiI ACCWGGT 1 cut(s) 997
Csp6I GTAC 2 cut(s) 190, 1294
CspAI ACCGGT 1 cut(s) 694
CviAII CATG 7 cut(s) 128, 217, 230, 598, 802, 856, 1208
CviQI GTAC 2 cut(s) 190, 1294
DdeI CTNAG 3 cut(s) 384, 960, 1059
DpnI GATC 8 cut(s) 71, 165, 240, 458, 648, 701, 1131, 1315
DpnII GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
DriI GACNNNNNGTC 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 946
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 946
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 432, 597
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 1310
Eco47I GGWCC 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 59, 997
EcoT14I CCWWGG 2 cut(s) 432, 597
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 2 cut(s) 432, 597
FaeI CATG 7 cut(s) 131, 220, 233, 601, 805, 859, 1211
FalI AAGNNNNNCTT 2 cut(s) 940, 972
FaqI GGGAC 1 cut(s) 328
FatI CATG 7 cut(s) 127, 216, 229, 597, 801, 855, 1207
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 2 cut(s) 238, 699
Fnu4HI GCNGC 4 cut(s) 328, 626, 677, 939
FokI GGATG 3 cut(s) 233, 275, 304
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 4 cut(s) 328, 626, 677, 939
FspBI CTAG 4 cut(s) 120, 401, 974, 1277
GlaI GCGC 2 cut(s) 580, 917
GluI GCNGC 4 cut(s) 328, 626, 677, 939
HaeIII GGCC 2 cut(s) 375, 453
HapII CCGG 3 cut(s) 367, 454, 695
HgaI GACGC 2 cut(s) 259, 451
HhaI GCGC 2 cut(s) 581, 918
Hin1II CATG 7 cut(s) 131, 220, 233, 601, 805, 859, 1211
Hin6I GCGC 2 cut(s) 579, 916
HinP1I GCGC 2 cut(s) 579, 916
HincII GTYRAC 2 cut(s) 112, 795
HindII GTYRAC 2 cut(s) 112, 795
HinfI GANTC 7 cut(s) 133, 428, 488, 509, 689, 1145, 1288
HpaII CCGG 3 cut(s) 367, 454, 695
HphI GGTGA 2 cut(s) 684, 709
Hpy166II GTNNAC 4 cut(s) 9, 112, 441, 795
Hpy188I TCNGA 7 cut(s) 202, 385, 576, 901, 961, 1031, 1313
Hpy188III TCNNGA 5 cut(s) 20, 67, 140, 650, 1323
Hpy8I GTNNAC 4 cut(s) 9, 112, 441, 795
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 5 cut(s) 117, 386, 615, 1180, 1278
HpyCH4III ACNGT 8 cut(s) 116, 210, 291, 335, 485, 540, 706, 1040
HpyCH4IV ACGT 5 cut(s) 474, 791, 1025, 1047, 1092
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 673
HpyF3I CTNAG 3 cut(s) 384, 960, 1059
HpySE526I ACGT 5 cut(s) 474, 791, 1025, 1047, 1092
Hsp92II CATG 7 cut(s) 131, 220, 233, 601, 805, 859, 1211
HspAI GCGC 2 cut(s) 579, 916
Ksp22I TGATCA 2 cut(s) 238, 699
Kzo9I GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 4 cut(s) 92, 576, 1228, 1272
Lsp1109I GCAGC 4 cut(s) 339, 612, 688, 925
LweI GCATC 2 cut(s) 637, 1099
MabI ACCWGGT 1 cut(s) 997
MaeI CTAG 4 cut(s) 120, 401, 974, 1277
MaeII ACGT 5 cut(s) 474, 791, 1025, 1047, 1092
MaeIII GTNAC 7 cut(s) 232, 291, 604, 1015, 1048, 1102, 1172
MalI GATC 8 cut(s) 71, 165, 240, 458, 648, 701, 1131, 1315
MboI GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
MboII GAAGA 6 cut(s) 34, 128, 189, 518, 963, 995
MfeI CAATTG 2 cut(s) 501, 1140
MflI RGATCY 3 cut(s) 163, 1129, 1313
MhlI GDGCHC 1 cut(s) 354
MlyI GAGTC 3 cut(s) 437, 503, 683
MmeI TCCRAC 1 cut(s) 345
MroXI GAANNNNTTC 2 cut(s) 955, 1094
MseI TTAA 7 cut(s) 168, 180, 257, 338, 542, 831, 1178
MslI CAYNNNNRTG 1 cut(s) 879
MspI CCGG 3 cut(s) 367, 454, 695
MspR9I CCNGG 2 cut(s) 61, 999
MunI CAATTG 2 cut(s) 501, 1140
MvaI CCWGG 2 cut(s) 61, 999
MvnI CGCG 2 cut(s) 581, 918
MwoI GCNNNNNNNGC 2 cut(s) 401, 673
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 1 cut(s) 1012
NdeII GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 7 cut(s) 131, 220, 233, 601, 805, 859, 1211
NlaIV GGNNCC 2 cut(s) 886, 1274
NmuCI GTSAC 5 cut(s) 232, 291, 1048, 1102, 1172
PaeR7I CTCGAG 1 cut(s) 140
PasI CCCWGGG 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 946
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 1044
PdmI GAANNNNTTC 2 cut(s) 955, 1094
PfeI GAWTC 4 cut(s) 133, 488, 1145, 1288
PflFI GACNNNGTC 1 cut(s) 999
PflMI CCANNNNNTGG 1 cut(s) 438
PinAI ACCGGT 1 cut(s) 694
PkrI GCNGC 4 cut(s) 329, 627, 678, 940
PleI GAGTC 3 cut(s) 436, 503, 683
PpsI GAGTC 3 cut(s) 436, 503, 683
PpuMI RGGWCCY 1 cut(s) 154
PshAI GACNNNNGTC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 354
Psp1406I AACGTT 2 cut(s) 1025, 1092
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 59, 997
PspGI CCWGG 2 cut(s) 59, 997
PspN4I GGNNCC 2 cut(s) 886, 1274
PspPI GGNCC 1 cut(s) 154
PspPPI RGGWCCY 1 cut(s) 154
PstI CTGCAG 2 cut(s) 329, 1129
PsuI RGATCY 3 cut(s) 163, 1129, 1313
PsyI GACNNNGTC 1 cut(s) 999
RsaI GTAC 2 cut(s) 191, 1295
RsaNI GTAC 2 cut(s) 190, 1294
RseI CAYNNNNRTG 1 cut(s) 879
SacI GAGCTC 1 cut(s) 354
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 7 cut(s) 168, 180, 257, 338, 542, 831, 1178
SatI GCNGC 4 cut(s) 328, 626, 677, 939
Sau3AI GATC 8 cut(s) 69, 163, 238, 456, 646, 699, 1129, 1313
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 3 cut(s) 437, 503, 683
ScrFI CCNGG 2 cut(s) 61, 999
SduI GDGCHC 1 cut(s) 354
SexAI ACCWGGT 1 cut(s) 997
SfaNI GCATC 2 cut(s) 637, 1099
SfcI CTRYAG 2 cut(s) 325, 1125
Sfr274I CTCGAG 1 cut(s) 140
SgrAI CRCCGGYG 1 cut(s) 694
SinI GGWCC 1 cut(s) 154
SlaI CTCGAG 1 cut(s) 140
SmiMI CAYNNNNRTG 1 cut(s) 879
SmlI CTYRAG 3 cut(s) 140, 347, 554
SmoI CTYRAG 3 cut(s) 140, 347, 554
SspMI CTAG 4 cut(s) 120, 401, 974, 1277
SstI GAGCTC 1 cut(s) 354
StyD4I CCNGG 2 cut(s) 59, 997
StyI CCWWGG 2 cut(s) 432, 597
TaaI ACNGT 8 cut(s) 116, 210, 291, 335, 485, 540, 706, 1040
TaiI ACGT 5 cut(s) 477, 794, 1028, 1050, 1095
TaqI TCGA 3 cut(s) 21, 72, 141
TaqII GACCGA 1 cut(s) 540
TatI WGTACW 1 cut(s) 1293
TfiI GAWTC 4 cut(s) 133, 488, 1145, 1288
Tru1I TTAA 7 cut(s) 168, 180, 257, 338, 542, 831, 1178
Tru9I TTAA 7 cut(s) 168, 180, 257, 338, 542, 831, 1178
TscAI CASTG 9 cut(s) 97, 121, 241, 361, 402, 711, 755, 883, 1155
TseFI GTSAC 5 cut(s) 232, 291, 1048, 1102, 1172
TseI GCWGC 4 cut(s) 327, 625, 676, 938
Tsp45I GTSAC 5 cut(s) 232, 291, 1048, 1102, 1172
TspDTI ATGAA 4 cut(s) 65, 205, 218, 266
TspGWI ACGGA 3 cut(s) 35, 176, 275
TspRI CASTG 9 cut(s) 97, 121, 241, 361, 402, 711, 755, 883, 1155
Tth111I GACNNNGTC 1 cut(s) 999
Van91I CCANNNNNTGG 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 154
XapI RAATTY 1 cut(s) 860
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 2 cut(s) 955, 1094
XspI CTAG 4 cut(s) 120, 401, 974, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.