Rmu_sc0006553.1_g000010

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006553.1
OG0012060
Physical Location & Seq
Forward (+)
76837 .. 79385
2549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006553.1_g000010.1.cds

Sequence Viewer

Length: 1476 bp
atggagtggactgcttgtcttgatgaatatgagaaactcgtcttccgaatgaacacccctagggtcgtgattgacaatgccgtctgctccactgcgactttagtcaaggtcgacagtgctagaaggcacggaattcttctagaggctgttcaggtcctcacagacctaaatctcaccattaaaaaagcttatatttcttctgacggacaatgggtcgtgattgacaatgccgtctgctccactgcgactttagtcaaggtcgacagtgctagaaggcacggaattcttctagaggctgttcaggtcctcacagacctaaatctcaccattaaaaaagcttatatttcttctgacggacaatggttcatggatgttttccatgttactgaccaaaatgggaacaaattaacggacgagagcgttatcaattacattgagcagtcactcggaacgattcacttcgggacctccaactgttctaacggcctaacagccctggagttaaccggaacggaccgagttggtcttctttccgaggtctttgcagtgctggctgagatgcaatgcaatgtggttgagtccaaggtatggacacacaacggccgaattgcttcactgatttatgtcaaggactgtggttcaggttgcccaattgaggactcccagaaaattgatacaattgaagcgcggttgaggactgtgctcaagggagacaatgacattcggagtgcgaaaacctcagtttccatggctgtcacgcacaccgaaagaagactccatcagatgatgtttgcagatcgcgattatgaaaggaagcccatcctacagctttgcactgattctccgattgtaacggtgcagaattgggctgaaaggagctattctgtagtgaatattcagtgcaaggatcgggcaaagcttttgtttgatgttgtttgcacaatgacagatatggactatgtggttttccatgccacgattaaaacagcgggagatagagcacacatggaattttacattaggcacactgatggaaccccaattagttctgaacccgaacgacatcgtgtaattcaatgcttacaagctgccattgaaagaagagcatctgagggtgtgagacttgaattgtgcaccaaggatagagaaggtctcctagcaaatgtcaccagaacattccgagaggccggtctcaatgtgacaagggccgagatatccacctcaacagacaaggctctaaacgttttctatgtcaccgatgcaattggaaaccctgcggatccagaaaccattgatgcagtgaggcagaaagtagggttgagtgacttgaaagtgaaggaattgccatccgtttgccattcagagggtgacagagaagagcaagcactaggggttggtggggcagtgctgttatcacttgggagcttagtgagaaggaatctatacaatttgggactcatcaggtctttttcttga

Protein Analysis

491

Amino Acids

54.55

Weight (kDa)

5.8

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 80, 230
AccB7I CCANNNNNTGG 1 cut(s) 588
AccI GTMKAC 2 cut(s) 111, 261
AccII CGCG 2 cut(s) 688, 801
AciI CCGC 3 cut(s) 688, 989, 1277
AclI AACGTT 1 cut(s) 1242
AclWI GGATC 3 cut(s) 915, 1274, 1287
AcoI YGGCCR 1 cut(s) 601
AcsI RAATTY 3 cut(s) 132, 282, 1010
AfiI CCNNNNNNNGG 3 cut(s) 588, 655, 1363
AgsI TTSAA 5 cut(s) 683, 1076, 1097, 1127, 1330
AhdI GACNNNNNGTC 2 cut(s) 15, 212
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 7 cut(s) 188, 338, 829, 879, 919, 1088, 1425
AluI AGCT 7 cut(s) 188, 338, 829, 879, 919, 1088, 1425
Alw21I GWGCWC 3 cut(s) 705, 1003, 1136
Alw26I GTCTC 4 cut(s) 705, 1114, 1157, 1196
Alw44I GTGCAC 1 cut(s) 1132
AlwI GGATC 3 cut(s) 915, 1274, 1287
AoxI GGCC 4 cut(s) 484, 601, 1185, 1206
ApaLI GTGCAC 1 cut(s) 1132
ApeKI GCWGC 1 cut(s) 1088
ApoI RAATTY 3 cut(s) 132, 282, 1010
Asp700I GAANNNNTTC 1 cut(s) 610
AspA2I CCTAGG 1 cut(s) 59
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 5 cut(s) 154, 304, 465, 514, 1206
AsuHPI GGTGA 5 cut(s) 166, 316, 1159, 1246, 1379
AvaII GGWCC 4 cut(s) 154, 304, 465, 514
AvrII CCTAGG 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 1136
BamHI GGATCC 1 cut(s) 1279
BbsI GAAGAC 3 cut(s) 34, 518, 778
Bbv12I GWGCWC 3 cut(s) 705, 1003, 1136
BbvI GCAGC 1 cut(s) 1075
BccI CCATC 4 cut(s) 786, 827, 1025, 1354
BceAI ACGGC 4 cut(s) 65, 215, 499, 616
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 1 cut(s) 497
BcoDI GTCTC 4 cut(s) 705, 1114, 1157, 1196
BfaI CTAG 7 cut(s) 60, 120, 140, 270, 290, 1157, 1388
BfmI CTRYAG 2 cut(s) 824, 885
BisI GCNGC 1 cut(s) 1089
BlnI CCTAGG 1 cut(s) 59
BlsI GCNGC 1 cut(s) 1090
Bme1390I CCNGG 1 cut(s) 497
Bme18I GGWCC 4 cut(s) 154, 304, 465, 514
BmeRI GACNNNNNGTC 2 cut(s) 15, 212
BmgT120I GGNCC 5 cut(s) 154, 304, 465, 514, 1206
BmiI GGNNCC 3 cut(s) 466, 1036, 1281
BmrFI CCNGG 1 cut(s) 497
BmsI GCATC 4 cut(s) 549, 1115, 1249, 1285
BoxI GACNNNNGTC 2 cut(s) 101, 251
BpiI GAAGAC 3 cut(s) 34, 518, 778
BplI GAGNNNNNCTC 2 cut(s) 1137, 1169
BpmI CTGGAG 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 689
BsaI GGTCTC 2 cut(s) 1157, 1196
BsaJI CCNNGG 6 cut(s) 59, 495, 534, 582, 747, 1137
BsaWI WCCGGW 1 cut(s) 506
BsaXI ACNNNNNCTCC 2 cut(s) 826, 856
Bsc4I CCNNNNNNNGG 3 cut(s) 588, 655, 1363
Bse118I RCCGGY 1 cut(s) 1187
Bse3DI GCAATG 2 cut(s) 569, 574
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 6 cut(s) 59, 495, 534, 582, 747, 1137
BseGI GGATG 3 cut(s) 376, 819, 1346
BseLI CCNNNNNNNGG 3 cut(s) 588, 655, 1363
BseMI GCAATG 2 cut(s) 569, 574
BseMII CTCAG 3 cut(s) 546, 753, 1101
BseSI GKGCMC 1 cut(s) 1136
BseX3I CGGCCG 1 cut(s) 601
BseXI GCAGC 1 cut(s) 1075
BsgI GTGCAG 1 cut(s) 878
Bsh1236I CGCG 2 cut(s) 688, 801
Bsh1285I CGRYCG 1 cut(s) 604
BshFI GGCC 4 cut(s) 486, 603, 1187, 1208
BsiEI CGRYCG 1 cut(s) 604
BsiHKAI GWGCWC 3 cut(s) 705, 1003, 1136
BsiSI CCGG 2 cut(s) 507, 1188
BslFI GGGAC 2 cut(s) 478, 1467
BslI CCNNNNNNNGG 3 cut(s) 588, 655, 1363
BsmAI GTCTC 4 cut(s) 705, 1114, 1157, 1196
BsmFI GGGAC 2 cut(s) 478, 1467
BsnI GGCC 4 cut(s) 486, 603, 1187, 1208
Bso31I GGTCTC 2 cut(s) 1157, 1196
Bsp1286I GDGCHC 3 cut(s) 705, 1003, 1136
Bsp143I GATC 3 cut(s) 796, 907, 1279
Bsp19I CCATGG 1 cut(s) 747
Bsp68I TCGCGA 1 cut(s) 801
BspACI CCGC 3 cut(s) 688, 989, 1277
BspANI GGCC 4 cut(s) 486, 603, 1187, 1208
BspCNI CTCAG 3 cut(s) 547, 752, 1102
BspFNI CGCG 2 cut(s) 688, 801
BspLI GGNNCC 3 cut(s) 466, 1036, 1281
BspPI GGATC 3 cut(s) 915, 1274, 1287
BspQI GCTCTTC 2 cut(s) 1096, 1371
BspTNI GGTCTC 2 cut(s) 1157, 1196
BsrDI GCAATG 2 cut(s) 569, 574
BsrFI RCCGGY 1 cut(s) 1187
BssAI RCCGGY 1 cut(s) 1187
BssECI CCNNGG 6 cut(s) 59, 495, 534, 582, 747, 1137
BssMI GATC 3 cut(s) 796, 907, 1279
BssT1I CCWWGG 4 cut(s) 59, 582, 747, 1137
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 6 cut(s) 116, 266, 476, 635, 700, 856
Bst6I CTCTTC 2 cut(s) 1096, 1371
BstC8I GCNNGC 2 cut(s) 552, 1383
BstDEI CTNAG 4 cut(s) 555, 739, 1110, 1426
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 376, 819, 1346
BstFNI CGCG 2 cut(s) 688, 801
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 3 cut(s) 799, 910, 1282
BstMAI GTCTC 4 cut(s) 705, 1114, 1157, 1196
BstMBI GATC 3 cut(s) 796, 907, 1279
BstMCI CGRYCG 1 cut(s) 604
BstMWI GCNNNNNNNGC 1 cut(s) 551
BstNI CCWGG 1 cut(s) 497
BstPAI GACNNNNGTC 2 cut(s) 101, 251
BstSCI CCNGG 1 cut(s) 495
BstSFI CTRYAG 2 cut(s) 824, 885
BstSLI GKGCMC 1 cut(s) 1136
BstUI CGCG 2 cut(s) 688, 801
BstV1I GCAGC 1 cut(s) 1075
BstV2I GAAGAC 3 cut(s) 34, 518, 778
BstX2I RGATCY 1 cut(s) 1279
BstYI RGATCY 1 cut(s) 1279
BstZI CGGCCG 1 cut(s) 601
BsuRI GGCC 4 cut(s) 486, 603, 1187, 1208
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 376, 819, 1346
BtsI GCAGTG 5 cut(s) 90, 240, 552, 1305, 1410
BtuMI TCGCGA 1 cut(s) 801
Cac8I GCNNGC 2 cut(s) 552, 1383
CfoI GCGC 1 cut(s) 688
Cfr10I RCCGGY 1 cut(s) 1187
Cfr13I GGNCC 5 cut(s) 154, 304, 465, 514, 1206
CpoI CGGWCCG 1 cut(s) 514
CspCI CAANNNNNGTGG 2 cut(s) 616, 651
CspI CGGWCCG 1 cut(s) 514
CviAII CATG 5 cut(s) 367, 380, 748, 971, 1006
DdeI CTNAG 4 cut(s) 555, 739, 1110, 1426
DpnI GATC 3 cut(s) 798, 909, 1281
DpnII GATC 3 cut(s) 796, 907, 1279
DrdI GACNNNNNNGTC 2 cut(s) 80, 230
DriI GACNNNNNGTC 2 cut(s) 15, 212
DseDI GACNNNNNNGTC 2 cut(s) 80, 230
EaeI YGGCCR 1 cut(s) 601
EagI CGGCCG 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 1096, 1371
Eam1105I GACNNNNNGTC 2 cut(s) 15, 212
EarI CTCTTC 2 cut(s) 1096, 1371
EclXI CGGCCG 1 cut(s) 601
Eco130I CCWWGG 4 cut(s) 59, 582, 747, 1137
Eco31I GGTCTC 2 cut(s) 1157, 1196
Eco32I GATATC 1 cut(s) 1215
Eco47I GGWCC 4 cut(s) 154, 304, 465, 514
Eco52I CGGCCG 1 cut(s) 601
EcoO109I RGGNCCY 3 cut(s) 154, 304, 465
EcoRI GAATTC 2 cut(s) 132, 282
EcoRII CCWGG 1 cut(s) 495
EcoRV GATATC 1 cut(s) 1215
EcoT14I CCWWGG 4 cut(s) 59, 582, 747, 1137
ErhI CCWWGG 4 cut(s) 59, 582, 747, 1137
FaeI CATG 5 cut(s) 370, 383, 751, 974, 1009
FaqI GGGAC 2 cut(s) 478, 1467
FatI CATG 5 cut(s) 366, 379, 747, 970, 1005
FauI CCCGC 1 cut(s) 982
FblI GTMKAC 2 cut(s) 111, 261
Fnu4HI GCNGC 1 cut(s) 1089
FokI GGATG 3 cut(s) 383, 806, 1333
Fsp4HI GCNGC 1 cut(s) 1089
FspBI CTAG 7 cut(s) 60, 120, 140, 270, 290, 1157, 1388
GlaI GCGC 1 cut(s) 687
GluI GCNGC 1 cut(s) 1089
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 4 cut(s) 486, 603, 1187, 1208
HapII CCGG 2 cut(s) 507, 1188
HhaI GCGC 1 cut(s) 688
Hin1II CATG 5 cut(s) 370, 383, 751, 974, 1009
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HincII GTYRAC 3 cut(s) 112, 262, 504
HindII GTYRAC 3 cut(s) 112, 262, 504
HindIII AAGCTT 3 cut(s) 186, 336, 917
HinfI GANTC 7 cut(s) 454, 578, 659, 774, 839, 1438, 1455
HpaI GTTAAC 1 cut(s) 504
HpaII CCGG 2 cut(s) 507, 1188
HphI GGTGA 5 cut(s) 166, 316, 1159, 1246, 1379
Hpy166II GTNNAC 5 cut(s) 9, 112, 262, 504, 1134
Hpy188III TCNNGA 9 cut(s) 20, 67, 140, 217, 290, 463, 800, 1283, 1473
Hpy8I GTNNAC 5 cut(s) 9, 112, 262, 504, 1134
HpyAV CCTTC 5 cut(s) 117, 267, 1142, 1330, 1428
HpyCH4III ACNGT 6 cut(s) 116, 266, 476, 635, 700, 856
HpyCH4IV ACGT 1 cut(s) 1242
HpyF10VI GCNNNNNNNGC 1 cut(s) 551
HpyF3I CTNAG 4 cut(s) 555, 739, 1110, 1426
HpySE526I ACGT 1 cut(s) 1242
Hsp92II CATG 5 cut(s) 370, 383, 751, 974, 1009
HspAI GCGC 1 cut(s) 686
KspAI GTTAAC 1 cut(s) 504
Kzo9I GATC 3 cut(s) 796, 907, 1279
LguI GCTCTTC 2 cut(s) 1096, 1371
LmnI GCTCC 4 cut(s) 92, 242, 876, 1422
Lsp1109I GCAGC 1 cut(s) 1075
LweI GCATC 4 cut(s) 549, 1115, 1249, 1285
MaeI CTAG 7 cut(s) 60, 120, 140, 270, 290, 1157, 1388
MaeII ACGT 1 cut(s) 1242
MaeIII GTNAC 9 cut(s) 382, 441, 754, 850, 1165, 1198, 1252, 1322, 1367
MalI GATC 3 cut(s) 798, 909, 1281
MboI GATC 3 cut(s) 796, 907, 1279
MboII GAAGA 9 cut(s) 34, 128, 189, 278, 339, 518, 783, 1113, 1388
MfeI CAATTG 3 cut(s) 651, 678, 1263
MflI RGATCY 1 cut(s) 1279
MhlI GDGCHC 3 cut(s) 705, 1003, 1136
MlyI GAGTC 4 cut(s) 587, 653, 768, 1449
MmeI TCCRAC 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 5 cut(s) 180, 330, 407, 503, 981
MslI CAYNNNNRTG 1 cut(s) 1029
MspA1I CMGCKG 1 cut(s) 989
MspI CCGG 2 cut(s) 507, 1188
MspR9I CCNGG 1 cut(s) 497
MunI CAATTG 3 cut(s) 651, 678, 1263
MvaI CCWGG 1 cut(s) 497
MvnI CGCG 2 cut(s) 688, 801
MwoI GCNNNNNNNGC 1 cut(s) 551
NcoI CCATGG 1 cut(s) 747
NdeII GATC 3 cut(s) 796, 907, 1279
NlaIII CATG 5 cut(s) 370, 383, 751, 974, 1009
NlaIV GGNNCC 3 cut(s) 466, 1036, 1281
NmeAIII GCCGAG 1 cut(s) 1234
NmuCI GTSAC 7 cut(s) 441, 754, 1165, 1198, 1252, 1322, 1367
NruI TCGCGA 1 cut(s) 801
PciSI GCTCTTC 2 cut(s) 1096, 1371
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 3 cut(s) 454, 839, 1438
PflMI CCANNNNNTGG 1 cut(s) 588
PkrI GCNGC 1 cut(s) 1090
PleI GAGTC 4 cut(s) 586, 653, 768, 1449
PpsI GAGTC 4 cut(s) 586, 653, 768, 1449
PpuMI RGGWCCY 3 cut(s) 154, 304, 465
PshAI GACNNNNGTC 2 cut(s) 101, 251
Psp1406I AACGTT 1 cut(s) 1242
Psp5II RGGWCCY 3 cut(s) 154, 304, 465
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 3 cut(s) 466, 1036, 1281
PspPI GGNCC 5 cut(s) 154, 304, 465, 514, 1206
PspPPI RGGWCCY 3 cut(s) 154, 304, 465
PsuI RGATCY 1 cut(s) 1279
RruI TCGCGA 1 cut(s) 801
RseI CAYNNNNRTG 1 cut(s) 1029
Rsr2I CGGWCCG 1 cut(s) 514
RsrII CGGWCCG 1 cut(s) 514
SalI GTCGAC 2 cut(s) 110, 260
SapI GCTCTTC 2 cut(s) 1096, 1371
SaqAI TTAA 5 cut(s) 180, 330, 407, 503, 981
SatI GCNGC 1 cut(s) 1089
Sau3AI GATC 3 cut(s) 796, 907, 1279
Sau96I GGNCC 5 cut(s) 154, 304, 465, 514, 1206
SchI GAGTC 4 cut(s) 587, 653, 768, 1449
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 3 cut(s) 705, 1003, 1136
SfaNI GCATC 4 cut(s) 549, 1115, 1249, 1285
SfcI CTRYAG 2 cut(s) 824, 885
SinI GGWCC 4 cut(s) 154, 304, 465, 514
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SsiI CCGC 3 cut(s) 688, 989, 1277
SspI AATATT 1 cut(s) 895
SspMI CTAG 7 cut(s) 60, 120, 140, 270, 290, 1157, 1388
StyD4I CCNGG 1 cut(s) 495
StyI CCWWGG 4 cut(s) 59, 582, 747, 1137
TaaI ACNGT 6 cut(s) 116, 266, 476, 635, 700, 856
TaiI ACGT 1 cut(s) 1245
TaqI TCGA 2 cut(s) 111, 261
TaqII GACCGA 1 cut(s) 531
TfiI GAWTC 3 cut(s) 454, 839, 1438
Tru1I TTAA 5 cut(s) 180, 330, 407, 503, 981
Tru9I TTAA 5 cut(s) 180, 330, 407, 503, 981
TseFI GTSAC 7 cut(s) 441, 754, 1165, 1198, 1252, 1322, 1367
TseI GCWGC 1 cut(s) 1088
Tsp45I GTSAC 7 cut(s) 441, 754, 1165, 1198, 1252, 1322, 1367
TspDTI ATGAA 4 cut(s) 39, 65, 355, 822
TspGWI ACGGA 7 cut(s) 144, 219, 294, 369, 425, 527, 1339
Van91I CCANNNNNTGG 1 cut(s) 588
VneI GTGCAC 1 cut(s) 1132
VpaK11BI GGWCC 4 cut(s) 154, 304, 465, 514
XapI RAATTY 3 cut(s) 132, 282, 1010
XbaI TCTAGA 2 cut(s) 139, 289
XmaJI CCTAGG 1 cut(s) 59
XmiI GTMKAC 2 cut(s) 111, 261
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 7 cut(s) 60, 120, 140, 270, 290, 1157, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.