MD00G1168800.v1.1

ACT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012060
Physical Location & Seq
Forward (+)
38865606 .. 38868599
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1168800.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGGAGTGGACTGCTTGCCTCGGCGAATATGAGAAGCTTGTCTTCCGGATGAACACTCCCAGGGTCGTGATCGACAATGCCGTCTGCTCCACTGCGACTTTAGTCAAGGTTGACAGTGCGAGAAGGCATGGAATTCTTCTGGAGGCTGTTCAGGTCCTCACGGATCTTAACCTCTCCATTAAAAAAGCTTACATTTCTTCTGATGGTCGCTGGTTCATGGATGTTTTTCATGTAACTGATCAGAACGGGAATAAATTAACAGACGAGAGCGTTATTAACTATATTGAGCAGTCACTTGGGACAATTCATTTTGGTTCATCCAACTGTAGTAACGGTCTTACGGCCCTTGAGCTCACCGGGACAGACCGAGTAGGCCTTCTTTCCGAGGTGTTTGCGGTGCTAGCTGACCTGCAATGCAATGTGGTTGAGTCCAAGGTGTGGACGCACAATGGCCGAATTGCTTCATTGATTTATGTTAAGGATTGTGATTCTGGTTGCCCGATTGAGGACTCACAGAAAATTGATAGGATTGAAGCGCGGTTAAGGAATGTCCTTAAAGGGGACAATGATATTCGAAGTGCGAAAACCTCTGTTTCAATGGCAGTTACACACACCGAAAGAAGACTGCATCAAATGATGTTTGTAGATCGCGATTATGACAGAAAGCCCGTTTTACAGCATTGCACCAATTCTCCGGTCATATCGGTGCAGAATTGGGCTGAAAGGGGTTATTCCATAGTGAATATTCAGTGCAAAGATAGGGCAAAGCTTTTGTTCGATGTTGTTTGCACGTTGACAGACATGGACTACGTGGTTTTTCATGCCACTATTAATACATCAGGGGACAGAGGATACATGGAATTTTACATTAGGCACACTGATGGAACCCCAATTAGCTCTGAACCCGAACAACAGCGTGTAATCCAATGCTTACAAGCCGCAGTTCAAAGAAGAGCATCTGAGGGTGTGAGGCTAGAACTATGCACCGAGGATAGACAAGGTCTTCTAGCTTATGTTACAAGGACATTCCGCGAGAACGGTCTAAACGTGACAAGGGCTGAGATATCCACGACAACGGACAAGGCGCTAAACGTATTCTACGTGACGGATGCAATGGGAAACCTTGCAGATGCGAAAACCATAGAATCAGTGAGACAGAAAATCGGGTTGAGTAACTTGAAAGTGAAGGAACTTCCATTGATTTACCATCAAAAGAGGGAAAGGGAGGAGCAGCAAGGAGTTGGTGGGGCAGTTTTGTTATCACTTGGGAGCCTAGTGAGAAGGAATCTGTACAATTTGGGGCTCATCAGATCTCATTCTTGA

Protein Analysis

441

Amino Acids

49.46

Weight (kDa)

6.61

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT PF01842 42 - 87 3.9e-06 ACT domain
ACT PF01842 115 - 175 9.5e-07 ACT domain
ACT_6 PF13740 117 - 154 1e-05 ACT domain
ACT_ACR9_3rd PF24931 234 - 316 2.5e-08 ACR9-like, ACT-like domain
ACT PF01842 246 - 296 5.4e-07 ACT domain
ACT PF01842 324 - 384 3.9e-08 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12420 AT1G12420
fragaria_vesca FvH4_5g00870 FvH4_5g00870
malus_domestica MD00G1083000.v1.1 MD00G1168800.v1.1
prunus_persica Prupe.5G081600_v2.0.a1
pyrus_communis pycom04g06870 pycom06g06450
rosa_chinensis RchiOBHm_Chr7g0201021
rosa_laevigata RLG00000003697
rosa_multiflora Rmu_sc0006553.1_g000010
rosa_roxburghii Rroxscaffold_3G00255590
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187200 Rh7BG190700 Rh7CG198200 Rh7DG192800
rosa_wichuraiana Rw7G016440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 3 cut(s) 538, 651, 1032
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 4 cut(s) 395, 538, 939, 1030
AclWI GGATC 1 cut(s) 171
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 2 cut(s) 132, 860
AfaI GTAC 1 cut(s) 1292
AfiI CCNNNNNNNGG 3 cut(s) 438, 505, 559
AgsI TTSAA 4 cut(s) 533, 597, 947, 1180
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 7 cut(s) 37, 188, 352, 404, 769, 897, 1010
AluI AGCT 7 cut(s) 37, 188, 352, 404, 769, 897, 1010
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 1147
AlwI GGATC 1 cut(s) 171
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 3 cut(s) 342, 373, 451
ApeKI GCWGC 1 cut(s) 1231
ApoI RAATTY 2 cut(s) 132, 860
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 460
AspLEI GCGC 2 cut(s) 538, 1087
AspS9I GGNCC 2 cut(s) 154, 343
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 346
AsuII TTCGAA 1 cut(s) 574
AsuNHI GCTAGC 1 cut(s) 400
AvaII GGWCC 1 cut(s) 154
BanII GRGCYC 2 cut(s) 354, 1305
BbsI GAAGAC 3 cut(s) 34, 628, 995
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 1243
BccI CCATC 3 cut(s) 197, 875, 1215
BceAI ACGGC 2 cut(s) 65, 357
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 845
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 1147
BfaI CTAG 4 cut(s) 401, 974, 1007, 1274
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 1088
BfuAI ACCTGC 1 cut(s) 417
BfuI GTATCC 1 cut(s) 845
BglII AGATCT 1 cut(s) 1310
BisI GCNGC 2 cut(s) 939, 1232
BlsI GCNGC 2 cut(s) 940, 1233
Bme1390I CCNGG 2 cut(s) 61, 358
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 2 cut(s) 154, 343
BmiI GGNNCC 2 cut(s) 886, 1271
BmrFI CCNGG 2 cut(s) 61, 358
BmsI GCATC 4 cut(s) 637, 965, 1099, 1120
BmtI GCTAGC 1 cut(s) 404
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 3 cut(s) 34, 628, 995
BpmI CTGGAG 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 574
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 358
BsaAI YACGTR 2 cut(s) 811, 1102
BsaJI CCNNGG 6 cut(s) 19, 59, 60, 384, 432, 987
BsaWI WCCGGW 2 cut(s) 45, 694
BsaXI ACNNNNNCTCC 2 cut(s) 676, 706
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 505, 559
Bse3DI GCAATG 4 cut(s) 419, 424, 679, 1119
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 6 cut(s) 19, 59, 60, 384, 432, 987
BseGI GGATG 4 cut(s) 54, 226, 317, 1114
BseLI CCNNNNNNNGG 3 cut(s) 438, 505, 559
BseMI GCAATG 4 cut(s) 419, 424, 679, 1119
BseMII CTCAG 2 cut(s) 951, 1050
BseRI GAGGAG 1 cut(s) 1241
BseXI GCAGC 1 cut(s) 1243
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 3 cut(s) 538, 651, 1032
BshFI GGCC 3 cut(s) 344, 375, 453
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 3 cut(s) 46, 357, 695
BslFI GGGAC 4 cut(s) 313, 373, 575, 857
BslI CCNNNNNNNGG 3 cut(s) 438, 505, 559
BsmAI GTCTC 1 cut(s) 1147
BsmFI GGGAC 4 cut(s) 313, 373, 575, 857
BsnI GGCC 3 cut(s) 344, 375, 453
Bsp119I TTCGAA 1 cut(s) 574
Bsp1286I GDGCHC 2 cut(s) 354, 1305
Bsp13I TCCGGA 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 1290
Bsp143I GATC 5 cut(s) 69, 163, 238, 646, 1310
Bsp68I TCGCGA 1 cut(s) 651
BspACI CCGC 4 cut(s) 395, 538, 939, 1030
BspANI GGCC 3 cut(s) 344, 375, 453
BspCNI CTCAG 2 cut(s) 952, 1051
BspEI TCCGGA 1 cut(s) 45
BspFNI CGCG 3 cut(s) 538, 651, 1032
BspLI GGNNCC 2 cut(s) 886, 1271
BspMI ACCTGC 1 cut(s) 417
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 171
BspQI GCTCTTC 1 cut(s) 946
BspT104I TTCGAA 1 cut(s) 574
BsrDI GCAATG 4 cut(s) 419, 424, 679, 1119
BsrGI TGTACA 1 cut(s) 1290
BssECI CCNNGG 6 cut(s) 19, 59, 60, 384, 432, 987
BssMI GATC 5 cut(s) 69, 163, 238, 646, 1310
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 4 cut(s) 116, 326, 335, 1040
Bst6I CTCTTC 1 cut(s) 946
BstAUI TGTACA 1 cut(s) 1290
BstBAI YACGTR 2 cut(s) 811, 1102
BstBI TTCGAA 1 cut(s) 574
BstC8I GCNNGC 2 cut(s) 16, 402
BstDEI CTNAG 2 cut(s) 960, 1059
BstF5I GGATG 4 cut(s) 54, 226, 317, 1114
BstFNI CGCG 3 cut(s) 538, 651, 1032
BstH2I RGCGCY 1 cut(s) 1088
BstHHI GCGC 2 cut(s) 538, 1087
BstKTI GATC 5 cut(s) 72, 166, 241, 649, 1313
BstMAI GTCTC 1 cut(s) 1147
BstMBI GATC 5 cut(s) 69, 163, 238, 646, 1310
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 1 cut(s) 61
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 59, 356
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 3 cut(s) 538, 651, 1032
BstV1I GCAGC 1 cut(s) 1243
BstV2I GAAGAC 3 cut(s) 34, 628, 995
BstX2I RGATCY 2 cut(s) 163, 1310
BstYI RGATCY 2 cut(s) 163, 1310
BsuI GTATCC 1 cut(s) 845
BsuRI GGCC 3 cut(s) 344, 375, 453
BtsCI GGATG 4 cut(s) 54, 226, 317, 1114
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 5 cut(s) 90, 121, 755, 876, 1155
BtuMI TCGCGA 1 cut(s) 651
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 16, 402
CfoI GCGC 2 cut(s) 538, 1087
Cfr13I GGNCC 2 cut(s) 154, 343
CseI GACGC 1 cut(s) 451
Csp6I GTAC 1 cut(s) 1291
CviAII CATG 6 cut(s) 128, 217, 230, 802, 821, 856
CviQI GTAC 1 cut(s) 1291
DdeI CTNAG 2 cut(s) 960, 1059
DpnI GATC 5 cut(s) 71, 165, 240, 648, 1312
DpnII GATC 5 cut(s) 69, 163, 238, 646, 1310
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 946
EarI CTCTTC 1 cut(s) 946
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 2 cut(s) 354, 1305
Eco32I GATATC 1 cut(s) 1065
Eco47I GGWCC 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 59
EcoRV GATATC 1 cut(s) 1065
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 2 cut(s) 354, 1305
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 6 cut(s) 131, 220, 233, 805, 824, 859
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 4 cut(s) 313, 373, 575, 857
FatI CATG 6 cut(s) 127, 216, 229, 801, 820, 855
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 2 cut(s) 939, 1232
FokI GGATG 4 cut(s) 61, 233, 304, 1121
FriOI GRGCYC 2 cut(s) 354, 1305
Fsp4HI GCNGC 2 cut(s) 939, 1232
FspBI CTAG 4 cut(s) 401, 974, 1007, 1274
GlaI GCGC 2 cut(s) 537, 1086
GluI GCNGC 2 cut(s) 939, 1232
GsuI CTGGAG 1 cut(s) 161
HaeII RGCGCY 1 cut(s) 1088
HaeIII GGCC 3 cut(s) 344, 375, 453
HapII CCGG 3 cut(s) 46, 357, 695
HgaI GACGC 1 cut(s) 451
HhaI GCGC 2 cut(s) 538, 1087
Hin1II CATG 6 cut(s) 131, 220, 233, 805, 824, 859
Hin6I GCGC 2 cut(s) 536, 1085
HinP1I GCGC 2 cut(s) 536, 1085
HincII GTYRAC 2 cut(s) 112, 795
HindII GTYRAC 2 cut(s) 112, 795
HindIII AAGCTT 3 cut(s) 35, 186, 767
HinfI GANTC 5 cut(s) 428, 488, 509, 1145, 1285
HpaII CCGG 3 cut(s) 46, 357, 695
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 4 cut(s) 9, 112, 441, 795
Hpy188I TCNGA 6 cut(s) 202, 243, 385, 901, 961, 1310
Hpy188III TCNNGA 5 cut(s) 46, 67, 140, 650, 1320
Hpy8I GTNNAC 4 cut(s) 9, 112, 441, 795
HpyAV CCTTC 4 cut(s) 117, 386, 1180, 1275
HpyCH4III ACNGT 4 cut(s) 116, 326, 335, 1040
HpyCH4IV ACGT 5 cut(s) 791, 810, 1047, 1092, 1101
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 2 cut(s) 960, 1059
HpySE526I ACGT 5 cut(s) 791, 810, 1047, 1092, 1101
Hsp92II CATG 6 cut(s) 131, 220, 233, 805, 824, 859
HspAI GCGC 2 cut(s) 536, 1085
Kpn2I TCCGGA 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 5 cut(s) 69, 163, 238, 646, 1310
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 3 cut(s) 92, 1228, 1269
Lsp1109I GCAGC 1 cut(s) 1243
LweI GCATC 4 cut(s) 637, 965, 1099, 1120
MaeI CTAG 4 cut(s) 401, 974, 1007, 1274
MaeII ACGT 5 cut(s) 791, 810, 1047, 1092, 1101
MaeIII GTNAC 8 cut(s) 232, 291, 329, 604, 1015, 1048, 1102, 1172
MalI GATC 5 cut(s) 71, 165, 240, 648, 1312
MboI GATC 5 cut(s) 69, 163, 238, 646, 1310
MboII GAAGA 6 cut(s) 34, 128, 189, 633, 963, 995
MflI RGATCY 2 cut(s) 163, 1310
MhlI GDGCHC 2 cut(s) 354, 1305
MlyI GAGTC 2 cut(s) 437, 503
MmeI TCCRAC 1 cut(s) 345
MroI TCCGGA 1 cut(s) 45
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 8 cut(s) 168, 180, 257, 276, 477, 542, 555, 831
MslI CAYNNNNRTG 2 cut(s) 704, 879
MspI CCGG 3 cut(s) 46, 357, 695
MspR9I CCNGG 2 cut(s) 61, 358
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 3 cut(s) 538, 651, 1032
MwoI GCNNNNNNNGC 1 cut(s) 401
NciI CCSGG 1 cut(s) 358
NdeII GATC 5 cut(s) 69, 163, 238, 646, 1310
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 6 cut(s) 131, 220, 233, 805, 824, 859
NlaIV GGNNCC 2 cut(s) 886, 1271
NmuCI GTSAC 3 cut(s) 291, 1048, 1102
NruI TCGCGA 1 cut(s) 651
NspV TTCGAA 1 cut(s) 574
PasI CCCWGGG 1 cut(s) 60
PceI AGGCCT 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 946
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 1044, 1098
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 3 cut(s) 488, 1145, 1285
PflFI GACNNNGTC 1 cut(s) 999
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 2 cut(s) 940, 1233
PleI GAGTC 2 cut(s) 436, 503
PpsI GAGTC 2 cut(s) 436, 503
Ppu21I YACGTR 2 cut(s) 811, 1102
PpuMI RGGWCCY 1 cut(s) 154
PshAI GACNNNNGTC 1 cut(s) 101
PshBI ATTAAT 1 cut(s) 831
Psp124BI GAGCTC 1 cut(s) 354
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 886, 1271
PspPI GGNCC 2 cut(s) 154, 343
PspPPI RGGWCCY 1 cut(s) 154
PsuI RGATCY 2 cut(s) 163, 1310
PsyI GACNNNGTC 1 cut(s) 999
RruI TCGCGA 1 cut(s) 651
RsaI GTAC 1 cut(s) 1292
RsaNI GTAC 1 cut(s) 1291
RseI CAYNNNNRTG 2 cut(s) 704, 879
SacI GAGCTC 1 cut(s) 354
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 8 cut(s) 168, 180, 257, 276, 477, 542, 555, 831
SatI GCNGC 2 cut(s) 939, 1232
Sau3AI GATC 5 cut(s) 69, 163, 238, 646, 1310
Sau96I GGNCC 2 cut(s) 154, 343
SchI GAGTC 2 cut(s) 437, 503
ScrFI CCNGG 2 cut(s) 61, 358
SduI GDGCHC 2 cut(s) 354, 1305
SfaNI GCATC 4 cut(s) 637, 965, 1099, 1120
SfcI CTRYAG 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 574
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 2 cut(s) 704, 879
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 4 cut(s) 395, 538, 939, 1030
SspI AATATT 1 cut(s) 745
SspMI CTAG 4 cut(s) 401, 974, 1007, 1274
SstI GAGCTC 1 cut(s) 354
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 59, 356
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 116, 326, 335, 1040
TaiI ACGT 5 cut(s) 794, 813, 1050, 1095, 1104
TaqI TCGA 3 cut(s) 72, 574, 777
TaqII GACCGA 1 cut(s) 381
TatI WGTACW 1 cut(s) 1290
TauI GCSGC 1 cut(s) 941
TfiI GAWTC 3 cut(s) 488, 1145, 1285
Tru1I TTAA 8 cut(s) 168, 180, 257, 276, 477, 542, 555, 831
Tru9I TTAA 8 cut(s) 168, 180, 257, 276, 477, 542, 555, 831
TscAI CASTG 5 cut(s) 97, 121, 755, 883, 1155
TseFI GTSAC 3 cut(s) 291, 1048, 1102
TseI GCWGC 1 cut(s) 1231
Tsp45I GTSAC 3 cut(s) 291, 1048, 1102
TspDTI ATGAA 7 cut(s) 65, 205, 218, 296, 306, 453, 809
TspGWI ACGGA 3 cut(s) 176, 1091, 1121
TspRI CASTG 5 cut(s) 97, 121, 755, 883, 1155
Tth111I GACNNNGTC 1 cut(s) 999
Van91I CCANNNNNTGG 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 154
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 2 cut(s) 132, 860
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 4 cut(s) 401, 974, 1007, 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.