AT1G13140

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013182
Physical Location & Seq
Reverse (-)
4478384 .. 4480327
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G13140.2

Sequence Viewer

Length: 1605 bp
ATGTCTGAAATATCATCTTCCATGCCTTTAACAGAGAGAGTTTATAATCATCTTTGTCTGTCCGATGTTTCTCTGGCCTTGTTGGGACTGTTTGTTTTCTGTTGTGTGCGTGAGAAGGTTACTAAAAAGCTGGGGCCAACGATATGGCCTGTGTTCGGAATTACTCCAGAGTTCTTCTTTCACAGGAATGATGTTTATGGTTGGGCTACAAGATGTTTGAAAAAATGTCGAGGTACCTTTCTCTATAACGGAATCTGGCTTGGTGGGTCGTATGGAGCTGTGACTTGTGTCCCTGCGAACGTTGAATACATGCTCAAGACTAACTTCAAGAACTTCCCAAAAGGTGCGTTTTTCAAAGAACGGTTCAATGATCTGCTCGAGGATGGTATCTTCAATGCTGATGCTGAGTCTTGGAAGGAGCAACGACGGATCATCATTACCGAAATGCATTCAACCAGGTTCGTGGAGCATTCCTTTCAGACAACACAAGATTTGGTAAGGAAGAAGCTGTTAAAGGTGATGGAGAGTTTCGCAAGATCACAAGAGGCTTTTGACCTTCAAGATGTGCTCTTGCGTTTGACATTTGACAACATCTGCATCGCCGGTCTAGGTGATGATCCAGGAACTCTGGATTCTGATCTCCCCCTTGTTCCATTCGCTCAAGCTTTTGAAGAAGCAACGGAATCTACTATGTTTAGATTCATGATTCCTCCTTTCATATGGAAACCCCTGAAGTTCTTCGACATCGGGTATGAGAAAGGTCTCAGGAAAGCTGTTGATGTCGTCCATGAGTTTGTCGACAAGATGGTTGTGGATCGTATCTGCAAGCTCAAAGAAGAAGGAACGTTAGGCAACAGATCCGATGTCCTCTCAAGGATTATTGAGATAGAAAGTCACAAGACGACTGATGAAAAAGATCCTTCTACCATCAAATTCTTTAGACAATTCTGCACAAGTTTCATCTTAGCTGGACGAGACACAAGTTCAGTTGCACTTACATGGTTCTTCTGGGTGATACAGAAACATCCAGAAGTCGAAAACAAAATCATCCGTGAGATAAGTGAAATCTTAAGACAGCGAGGGGATTCTCCAACCAGCAAAAACGAGAGTCTCTTCACGGTCAAAGAACTAAACGACATGGTATATCTACAAGCAGCACTTTCAGAAACAATGAGACTTTACCCTCCAATACCAATGGAAATGAAACAAGCCATCGAAGACGATGTGTTTCCCGATGGGACATTCATACGTAAAGGCTCACGGGTTTACTTTGCGACTTACGCCATGGGAAGGATGGAATCCATATGGGGAAAAGACTGTGAATCATTCAAACCAGAGAGATGGATCCAATCAGGGAATTTCGTGAATGATGACCAATTTAAATACGTTGTGTTCAATGCTGGACCAAGGCTGTGTTTAGGGAAAACATTTGCTTACCTACAGATGAAGACAATCGCTGCTTCAGTCCTGTCTAGGTACTCAATAAAGGTTGCTAAAGATCATGTAGTTGTCCCCAGAGTAACTACAACCTTGTACATGAGGCACGGTCTCAAGGTAACCATCTCGTCAAAGTCCCTTGAAGAGAAGATACACGTTCAAGACTAG

Protein Analysis

534

Amino Acids

61.5

Weight (kDa)

7.91

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 45 - 241 7.7e-12 Cytochrome P450
p450 PF00067 259 - 482 1.3e-47 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13140 AT1G13140 AT1G13150 AT1G24540 AT3G26125
fragaria_vesca FvH4_4g25350
malus_domestica MD13G1104800.v1.1
prunus_persica Prupe.1G241700_v2.0.a1
pyrus_communis pycom13g09100
rosa_chinensis RchiOBHm_Chr4g0432631
rosa_laevigata RLG00000006828
rosa_multiflora Rmu_sc0004612.1_g000006
rosa_roxburghii Rroxscaffold_5G00374100
rosa_rugosa Rorug04G0260900.1
rosa_samantha Rh4BG323500 Rh4CG338600 Rh4DG319100
rosa_wichuraiana Rw4G027440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 45
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 798
AclI AACGTT 2 cut(s) 300, 845
AclWI GGATC 7 cut(s) 437, 611, 822, 852, 911, 1339, 1352
AcsI RAATTY 2 cut(s) 932, 1357
AcuI CTGAAG 2 cut(s) 752, 1446
AfaI GTAC 3 cut(s) 235, 1478, 1535
AfiI CCNNNNNNNGG 2 cut(s) 155, 1290
AflII CTTAAG 1 cut(s) 1069
AflIII ACRYGT 1 cut(s) 1591
AjnI CCWGG 2 cut(s) 455, 619
AluBI AGCT 7 cut(s) 130, 278, 508, 665, 773, 829, 968
AluI AGCT 7 cut(s) 130, 278, 508, 665, 773, 829, 968
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 5 cut(s) 767, 969, 1115, 1168, 1553
AlwI GGATC 7 cut(s) 437, 611, 822, 852, 911, 1339, 1352
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 3 cut(s) 75, 134, 146
ApeKI GCWGC 2 cut(s) 1154, 1457
ApoI RAATTY 2 cut(s) 932, 1357
Asp700I GAANNNNTTC 1 cut(s) 737
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 134, 1403
AsuHPI GGTGA 3 cut(s) 529, 623, 1024
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 1403
BamHI GGATCC 1 cut(s) 1344
BanI GGYRCC 1 cut(s) 233
BarI GAAGNNNNNNTAC 2 cut(s) 1572, 1604
BbsI GAAGAC 2 cut(s) 1224, 1454
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 2 cut(s) 1166, 1444
BccI CCATC 9 cut(s) 377, 514, 799, 935, 1220, 1229, 1288, 1335, 1568
BciT130I CCWGG 2 cut(s) 457, 621
BcoDI GTCTC 5 cut(s) 767, 969, 1115, 1168, 1553
BfaI CTAG 3 cut(s) 608, 1473, 1603
BfmI CTRYAG 1 cut(s) 1439
BfrI CTTAAG 1 cut(s) 1069
BisI GCNGC 2 cut(s) 1155, 1458
BlsI GCNGC 2 cut(s) 1156, 1459
Bme1390I CCNGG 2 cut(s) 457, 621
Bme18I GGWCC 1 cut(s) 1403
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 2 cut(s) 134, 1403
BmiI GGNNCC 3 cut(s) 135, 235, 1346
BmrFI CCNGG 2 cut(s) 457, 621
BmsI GCATC 2 cut(s) 391, 606
BoxI GACNNNNGTC 1 cut(s) 287
BpiI GAAGAC 2 cut(s) 1224, 1454
BpmI CTGGAG 1 cut(s) 150
BpuEI CTTGAG 4 cut(s) 299, 645, 856, 1535
BsaAI YACGTR 1 cut(s) 1250
BsaBI GATNNNNATC 1 cut(s) 636
BsaI GGTCTC 2 cut(s) 767, 1553
BsaJI CCNNGG 2 cut(s) 1284, 1406
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 1290
Bse118I RCCGGY 1 cut(s) 602
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 2 cut(s) 457, 621
BseDI CCNNGG 2 cut(s) 1284, 1406
BseGI GGATG 4 cut(s) 388, 1024, 1047, 1299
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 155, 1290
BseMII CTCAG 2 cut(s) 396, 778
BseXI GCAGC 2 cut(s) 1166, 1444
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 934
BshFI GGCC 3 cut(s) 77, 136, 148
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 603
BslFI GGGAC 5 cut(s) 99, 275, 1252, 1496, 1558
BslI CCNNNNNNNGG 2 cut(s) 155, 1290
BsmAI GTCTC 5 cut(s) 767, 969, 1115, 1168, 1553
BsmFI GGGAC 5 cut(s) 99, 275, 1252, 1496, 1558
BsmI GAATGC 2 cut(s) 448, 469
BsnI GGCC 3 cut(s) 77, 136, 148
Bso31I GGTCTC 2 cut(s) 767, 1553
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 570
Bsp1407I TGTACA 1 cut(s) 1533
Bsp19I CCATGG 1 cut(s) 1284
BspANI GGCC 3 cut(s) 77, 136, 148
BspCNI CTCAG 2 cut(s) 397, 777
BspHI TCATGA 1 cut(s) 702
BspLI GGNNCC 3 cut(s) 135, 235, 1346
BspPI GGATC 7 cut(s) 437, 611, 822, 852, 911, 1339, 1352
BspT107I GGYRCC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 1069
BspTNI GGTCTC 2 cut(s) 767, 1553
BsrFI RCCGGY 1 cut(s) 602
BsrGI TGTACA 1 cut(s) 1533
BssAI RCCGGY 1 cut(s) 602
BssECI CCNNGG 2 cut(s) 1284, 1406
BssT1I CCWWGG 2 cut(s) 1284, 1406
Bst2UI CCWGG 2 cut(s) 457, 621
Bst4CI ACNGT 5 cut(s) 90, 363, 1120, 1319, 1547
Bst6I CTCTTC 2 cut(s) 1118, 1575
BstAFI CTTAAG 1 cut(s) 1069
BstAUI TGTACA 1 cut(s) 1533
BstBAI YACGTR 1 cut(s) 1250
BstC8I GCNNGC 1 cut(s) 827
BstDEI CTNAG 3 cut(s) 405, 764, 964
BstDSI CCRYGG 1 cut(s) 1284
BstEII GGTNACC 1 cut(s) 1555
BstF5I GGATG 4 cut(s) 388, 1024, 1047, 1299
BstMAI GTCTC 5 cut(s) 767, 969, 1115, 1168, 1553
BstMWI GCNNNNNNNGC 1 cut(s) 1280
BstNI CCWGG 2 cut(s) 457, 621
BstNSI RCATGY 1 cut(s) 313
BstPAI GACNNNNGTC 1 cut(s) 287
BstPI GGTNACC 1 cut(s) 1555
BstSCI CCNGG 2 cut(s) 455, 619
BstSFI CTRYAG 1 cut(s) 1439
BstSNI TACGTA 1 cut(s) 1250
BstV1I GCAGC 2 cut(s) 1166, 1444
BstV2I GAAGAC 2 cut(s) 1224, 1454
BstX2I RGATCY 3 cut(s) 857, 916, 1344
BstXI CCANNNNNNTGG 3 cut(s) 144, 463, 1341
BstYI RGATCY 3 cut(s) 857, 916, 1344
BsuRI GGCC 3 cut(s) 77, 136, 148
BtgI CCRYGG 1 cut(s) 1284
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 4 cut(s) 388, 1024, 1047, 1299
Cac8I GCNNGC 1 cut(s) 827
CciI TCATGA 1 cut(s) 702
Cfr10I RCCGGY 1 cut(s) 602
Cfr13I GGNCC 2 cut(s) 134, 1403
CsiI ACCWGGT 1 cut(s) 455
Csp6I GTAC 3 cut(s) 234, 1477, 1534
CviAII CATG 9 cut(s) 22, 310, 703, 788, 999, 1138, 1285, 1502, 1537
CviQI GTAC 3 cut(s) 234, 1477, 1534
DdeI CTNAG 3 cut(s) 405, 764, 964
DraI TTTAAA 1 cut(s) 1381
Eam1104I CTCTTC 2 cut(s) 1118, 1575
EarI CTCTTC 2 cut(s) 1118, 1575
Eco105I TACGTA 1 cut(s) 1250
Eco130I CCWWGG 2 cut(s) 1284, 1406
Eco31I GGTCTC 2 cut(s) 767, 1553
Eco47I GGWCC 1 cut(s) 1403
Eco57I CTGAAG 2 cut(s) 752, 1446
Eco88I CYCGRG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 1555
EcoO65I GGTNACC 1 cut(s) 1555
EcoRII CCWGG 2 cut(s) 455, 619
EcoT14I CCWWGG 2 cut(s) 1284, 1406
EcoT22I ATGCAT 1 cut(s) 450
ErhI CCWWGG 2 cut(s) 1284, 1406
FaeI CATG 9 cut(s) 25, 313, 706, 791, 1002, 1141, 1288, 1505, 1540
FalI AAGNNNNNCTT 4 cut(s) 308, 340, 1143, 1175
FaqI GGGAC 5 cut(s) 99, 275, 1252, 1496, 1558
FatI CATG 9 cut(s) 21, 309, 702, 787, 998, 1137, 1284, 1501, 1536
FauNDI CATATG 2 cut(s) 719, 1304
FblI GTMKAC 1 cut(s) 798
Fnu4HI GCNGC 2 cut(s) 1155, 1458
FokI GGATG 4 cut(s) 395, 1011, 1034, 1306
Fsp4HI GCNGC 2 cut(s) 1155, 1458
FspBI CTAG 3 cut(s) 608, 1473, 1603
GluI GCNGC 2 cut(s) 1155, 1458
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 3 cut(s) 77, 136, 148
HapII CCGG 1 cut(s) 603
Hin1II CATG 9 cut(s) 25, 313, 706, 791, 1002, 1141, 1288, 1505, 1540
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 1 cut(s) 663
HpaII CCGG 1 cut(s) 603
HphI GGTGA 3 cut(s) 529, 623, 1024
Hpy166II GTNNAC 2 cut(s) 799, 1267
Hpy188I TCNGA 7 cut(s) 7, 64, 158, 480, 637, 862, 1165
Hpy8I GTNNAC 2 cut(s) 799, 1267
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 6 cut(s) 109, 409, 566, 833, 930, 1284
HpyCH4III ACNGT 5 cut(s) 90, 363, 1120, 1319, 1547
HpyCH4IV ACGT 5 cut(s) 300, 845, 1249, 1386, 1593
HpyCH4V TGCA 5 cut(s) 448, 597, 825, 951, 992
HpyF10VI GCNNNNNNNGC 1 cut(s) 1280
HpyF3I CTNAG 3 cut(s) 405, 764, 964
HpySE526I ACGT 5 cut(s) 300, 845, 1249, 1386, 1593
Hsp92II CATG 9 cut(s) 25, 313, 706, 791, 1002, 1141, 1288, 1505, 1540
KpnI GGTACC 1 cut(s) 237
LmnI GCTCC 3 cut(s) 275, 418, 466
Lsp1109I GCAGC 2 cut(s) 1166, 1444
LweI GCATC 2 cut(s) 391, 606
MabI ACCWGGT 1 cut(s) 455
MaeI CTAG 3 cut(s) 608, 1473, 1603
MaeII ACGT 5 cut(s) 300, 845, 1249, 1386, 1593
MaeIII GTNAC 5 cut(s) 118, 280, 893, 1519, 1555
MflI RGATCY 3 cut(s) 857, 916, 1344
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 5 cut(s) 159, 932, 944, 1357, 1376
MlyI GAGTC 2 cut(s) 416, 1117
MmeI TCCRAC 1 cut(s) 1115
MnlI CCTC 8 cut(s) 224, 373, 538, 720, 878, 1073, 1194, 1533
Mph1103I ATGCAT 1 cut(s) 450
MroXI GAANNNNTTC 1 cut(s) 737
MseI TTAA 4 cut(s) 29, 512, 1070, 1380
MslI CAYNNNNRTG 2 cut(s) 186, 997
MspCI CTTAAG 1 cut(s) 1069
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 2 cut(s) 457, 621
Mva1269I GAATGC 2 cut(s) 448, 469
MvaI CCWGG 2 cut(s) 457, 621
MwoI GCNNNNNNNGC 1 cut(s) 1280
NcoI CCATGG 1 cut(s) 1284
NdeI CATATG 2 cut(s) 719, 1304
NlaIII CATG 9 cut(s) 25, 313, 706, 791, 1002, 1141, 1288, 1505, 1540
NlaIV GGNNCC 3 cut(s) 135, 235, 1346
NmuCI GTSAC 2 cut(s) 280, 893
NsiI ATGCAT 1 cut(s) 450
NspI RCATGY 1 cut(s) 313
PaeR7I CTCGAG 1 cut(s) 377
PagI TCATGA 1 cut(s) 702
PctI GAATGC 2 cut(s) 448, 469
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 8 cut(s) 252, 632, 683, 699, 706, 1085, 1298, 1322
PfoI TCCNGGA 1 cut(s) 619
PkrI GCNGC 2 cut(s) 1156, 1459
PleI GAGTC 2 cut(s) 415, 1116
PpsI GAGTC 2 cut(s) 415, 1116
Ppu21I YACGTR 1 cut(s) 1250
PshAI GACNNNNGTC 1 cut(s) 287
PsiI TTATAA 1 cut(s) 45
Psp1406I AACGTT 2 cut(s) 300, 845
Psp6I CCWGG 2 cut(s) 455, 619
PspEI GGTNACC 1 cut(s) 1555
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 455, 619
PspN4I GGNNCC 3 cut(s) 135, 235, 1346
PspPI GGNCC 2 cut(s) 134, 1403
PspXI VCTCGAGB 1 cut(s) 377
PsrI GAACNNNNNNTAC 2 cut(s) 290, 322
PsuI RGATCY 3 cut(s) 857, 916, 1344
RsaI GTAC 3 cut(s) 235, 1478, 1535
RsaNI GTAC 3 cut(s) 234, 1477, 1534
RseI CAYNNNNRTG 2 cut(s) 186, 997
SalI GTCGAC 1 cut(s) 797
SaqAI TTAA 4 cut(s) 29, 512, 1070, 1380
SatI GCNGC 2 cut(s) 1155, 1458
Sau96I GGNCC 2 cut(s) 134, 1403
SchI GAGTC 2 cut(s) 416, 1117
ScrFI CCNGG 2 cut(s) 457, 621
SduI GDGCHC 1 cut(s) 570
SexAI ACCWGGT 1 cut(s) 455
SfaNI GCATC 2 cut(s) 391, 606
SfcI CTRYAG 1 cut(s) 1439
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 1 cut(s) 1403
SlaI CTCGAG 1 cut(s) 377
SmiI ATTTAAAT 1 cut(s) 1381
SmiMI CAYNNNNRTG 2 cut(s) 186, 997
SmlI CTYRAG 6 cut(s) 314, 377, 660, 871, 1069, 1550
SmoI CTYRAG 6 cut(s) 314, 377, 660, 871, 1069, 1550
SnaBI TACGTA 1 cut(s) 1250
Sse9I AATT 5 cut(s) 159, 932, 944, 1357, 1376
SspMI CTAG 3 cut(s) 608, 1473, 1603
StyD4I CCNGG 2 cut(s) 455, 619
StyI CCWWGG 2 cut(s) 1284, 1406
SwaI ATTTAAAT 1 cut(s) 1381
TaaI ACNGT 5 cut(s) 90, 363, 1120, 1319, 1547
TaiI ACGT 5 cut(s) 303, 848, 1252, 1389, 1596
TaqI TCGA 6 cut(s) 229, 378, 741, 798, 1035, 1215
TasI AATT 5 cut(s) 159, 932, 944, 1357, 1376
TatI WGTACW 1 cut(s) 1533
TfiI GAWTC 8 cut(s) 252, 632, 683, 699, 706, 1085, 1298, 1322
Tru1I TTAA 4 cut(s) 29, 512, 1070, 1380
Tru9I TTAA 4 cut(s) 29, 512, 1070, 1380
TseFI GTSAC 2 cut(s) 280, 893
TseI GCWGC 2 cut(s) 1154, 1457
Tsp45I GTSAC 2 cut(s) 280, 893
TspDTI ATGAA 7 cut(s) 691, 706, 924, 949, 1217, 1234, 1460
TspGWI ACGGA 4 cut(s) 264, 442, 695, 1040
Vha464I CTTAAG 1 cut(s) 1069
VpaK11BI GGWCC 1 cut(s) 1403
XapI RAATTY 2 cut(s) 932, 1357
XceI RCATGY 1 cut(s) 313
XcmI CCANNNNNNNNNTGG 1 cut(s) 1291
XhoI CTCGAG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 798
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 3 cut(s) 608, 1473, 1603
Zsp2I ATGCAT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.