RchiOBHm_Chr4g0432631

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013182
Physical Location & Seq
Forward (+)
56761501 .. 56762741
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40120

Sequence Viewer

Length: 1134 bp
ATGGGTTTTTCCTCTGCAATGATTCTGAACTGGCTACATTTCTGGGATGTAGCACTTGCCTTGATGGGCCTGTTCATATTTAGTTGCTTGCTTCAGAGGTTCACCAACAAAAATGGTCCAATGTTATGGCCAGTTTTGGGGATTGCACCCTCCATATTTCTAAATGCACACACCATCTATGATTGGGTTACCATGGCTCTGATCAAAGCAGGGGGAACATTCCACTACAAAGGAGTATGGTTCGGCGGGTCCCACGGGATCATAACACTTGATCCTTCAAACATTGAGTACATGCTCAAAACAAGGTTCAGCAACTTCCCAAAAGGCAAGTATTACAGGGAGAGGTTTCATGATTTGCTTGGGGATGGAATTTTCAATGCAGACAATGAGTTGTGGAAGGAGCAGAGGAGGTTGGCAACTTCAGAAATGCATTCGGGTCGTTTTGTAGAGTATTCGTTTTTCTCCATGCAAGAGTTGGTGCACAAGAAGTTGTTGAAGTTGATGGAGAAAGTGGTAGTATCAGGGTGTAGCATTGATCTTCATGAAGTGCTTCTTCGGTTTACTTTTGACAACATTTGCTCGGTAGCTCTTGGTGCTGATTCAGGGTGTTTGGAGCTTGATTTGCCTCAAAGTCCTTTTGTGAAAGCCTTTGAAGAAGCCACGGAGCTCACTATGTTCAGATTCATGGTGCCACCTTTTGTGTGGAAGCCTATGAAGTTGTTTGGAATTGGGTTCGAAAAGAGGCTCAAGGAAGCAACTAAAATAGTGCATTATTTTGCTGAAAATACGGTGAAGGATCGAAGGGGAGAATTGAAGAGGCTCGGTAGCTTAAAAGATCGTTTTGATCTATTGTCAAGGCTTATGGACAGTGAAAAGCAACATTTTTCGGATAAGTTTCTAAGCGATTTCTGCATAAGTTTCATTTTGGCTGGAAGGGACACAAGCTCTGTAGGATTGGCATGGTTTTTTTGGTTGGTACAAAAACATCCTGAAGTGGAAAACAAAATCCTCAAAGAAATCAATGAAATTTTAGGCCAGCGCCAATGTACCGAAAAAGAATCACCAGATGGTGATACTGTTTTTACAGTGGAGGAACTCAAGAAGATGGTCTATTTACAAGCAGTGTTGTCGTAG

Protein Analysis

377

Amino Acids

43.52

Weight (kDa)

7.17

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 77 - 376 3.5e-40 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13140 AT1G13140 AT1G13150 AT1G24540 AT3G26125
fragaria_vesca FvH4_4g25350
malus_domestica MD13G1104800.v1.1
prunus_persica Prupe.1G241700_v2.0.a1
pyrus_communis pycom13g09100
rosa_chinensis RchiOBHm_Chr4g0432631
rosa_laevigata RLG00000006828
rosa_multiflora Rmu_sc0004612.1_g000006
rosa_roxburghii Rroxscaffold_5G00374100
rosa_rugosa Rorug04G0260900.1
rosa_samantha Rh4BG323500 Rh4CG338600 Rh4DG319100
rosa_wichuraiana Rw4G027440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 688
AciI CCGC 1 cut(s) 246
AclWI GGATC 3 cut(s) 266, 266, 804
AcoI YGGCCR 1 cut(s) 128
AcsI RAATTY 2 cut(s) 369, 1026
AcuI CTGAAG 3 cut(s) 77, 405, 1011
AfaI GTAC 3 cut(s) 290, 978, 1048
AfiI CCNNNNNNNGG 1 cut(s) 137
AgsI TTSAA 5 cut(s) 279, 376, 496, 653, 814
AluBI AGCT 5 cut(s) 587, 616, 667, 828, 945
AluI AGCT 5 cut(s) 587, 616, 667, 828, 945
Alw21I GWGCWC 2 cut(s) 483, 669
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 3 cut(s) 266, 266, 804
AoxI GGCC 3 cut(s) 67, 128, 1033
ApaLI GTGCAC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 369, 1026
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 1 cut(s) 1041
AspS9I GGNCC 3 cut(s) 67, 116, 249
AsuHPI GGTGA 4 cut(s) 94, 802, 1053, 1082
AsuII TTCGAA 1 cut(s) 735
AvaII GGWCC 2 cut(s) 116, 249
BaeGI GKGCMC 1 cut(s) 483
BalI TGGCCA 1 cut(s) 130
BanI GGYRCC 1 cut(s) 688
BanII GRGCYC 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 483, 669
BccI CCATC 6 cut(s) 58, 182, 359, 496, 1061, 1099
BcgI CGANNNNNNTGC 3 cut(s) 419, 453, 1110
BclI TGATCA 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 948
BfoI RGCGCY 1 cut(s) 1042
Bme18I GGWCC 2 cut(s) 116, 249
BmgT120I GGNCC 3 cut(s) 67, 116, 249
BmiI GGNNCC 3 cut(s) 250, 251, 690
Bpu14I TTCGAA 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 731, 1082
BsaJI CCNNGG 3 cut(s) 192, 253, 660
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 35, 131
Bse3DI GCAATG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 192, 253, 660
BseGI GGATG 3 cut(s) 52, 370, 985
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 24
BseNI ACTGG 2 cut(s) 35, 131
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 1 cut(s) 483
BshFI GGCC 3 cut(s) 69, 130, 1035
BshNI GGYRCC 1 cut(s) 688
BsiHKAI GWGCWC 2 cut(s) 483, 669
BslFI GGGAC 2 cut(s) 235, 950
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 2 cut(s) 235, 950
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 3 cut(s) 69, 130, 1035
Bsp119I TTCGAA 1 cut(s) 735
Bsp1286I GDGCHC 2 cut(s) 483, 669
Bsp143I GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
Bsp19I CCATGG 1 cut(s) 192
BspACI CCGC 1 cut(s) 246
BspANI GGCC 3 cut(s) 69, 130, 1035
BspHI TCATGA 2 cut(s) 349, 541
BspLI GGNNCC 3 cut(s) 250, 251, 690
BspPI GGATC 3 cut(s) 266, 266, 804
BspT104I TTCGAA 1 cut(s) 735
BspT107I GGYRCC 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 24
BsrI ACTGG 2 cut(s) 35, 131
BssECI CCNNGG 3 cut(s) 192, 253, 660
BssMI GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
BssT1I CCWWGG 1 cut(s) 192
Bst4CI ACNGT 4 cut(s) 790, 869, 1078, 1087
Bst6I CTCTTC 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 735
BstC8I GCNNGC 2 cut(s) 89, 1037
BstDEI CTNAG 1 cut(s) 899
BstDSI CCRYGG 3 cut(s) 192, 253, 660
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 3 cut(s) 52, 370, 985
BstH2I RGCGCY 1 cut(s) 1042
BstHHI GCGC 1 cut(s) 1041
BstKTI GATC 7 cut(s) 204, 261, 274, 538, 799, 838, 847
BstMBI GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
BstMWI GCNNNNNNNGC 3 cut(s) 593, 622, 909
BstNSI RCATGY 1 cut(s) 295
BstPI GGTNACC 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 948
BstSLI GKGCMC 1 cut(s) 483
BstXI CCANNNNNNTGG 1 cut(s) 126
BsuRI GGCC 3 cut(s) 69, 130, 1035
BtgI CCRYGG 3 cut(s) 192, 253, 660
BtsCI GGATG 3 cut(s) 52, 370, 985
BtsI GCAGTG 1 cut(s) 1128
BtsIMutI CAGTG 3 cut(s) 874, 1092, 1128
Cac8I GCNNGC 2 cut(s) 89, 1037
CciI TCATGA 2 cut(s) 349, 541
CfoI GCGC 1 cut(s) 1041
Cfr13I GGNCC 3 cut(s) 67, 116, 249
Csp6I GTAC 3 cut(s) 289, 977, 1047
CviAII CATG 7 cut(s) 193, 292, 350, 466, 542, 685, 960
CviQI GTAC 3 cut(s) 289, 977, 1047
DdeI CTNAG 1 cut(s) 899
DpnI GATC 7 cut(s) 203, 260, 273, 537, 798, 837, 846
DpnII GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
EaeI YGGCCR 1 cut(s) 128
Eam1104I CTCTTC 1 cut(s) 809
EarI CTCTTC 1 cut(s) 809
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 669
Eco47I GGWCC 2 cut(s) 116, 249
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 3 cut(s) 77, 405, 1011
Eco91I GGTNACC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 249
EcoO65I GGTNACC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 7 cut(s) 196, 295, 353, 469, 545, 688, 963
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 2 cut(s) 235, 950
FatI CATG 7 cut(s) 192, 291, 349, 465, 541, 684, 959
FauI CCCGC 1 cut(s) 239
FbaI TGATCA 1 cut(s) 201
FokI GGATG 3 cut(s) 59, 377, 972
FriOI GRGCYC 1 cut(s) 669
GlaI GCGC 1 cut(s) 1040
HaeII RGCGCY 1 cut(s) 1042
HaeIII GGCC 3 cut(s) 69, 130, 1035
HhaI GCGC 1 cut(s) 1041
Hin1II CATG 7 cut(s) 196, 295, 353, 469, 545, 688, 963
Hin6I GCGC 1 cut(s) 1039
HinP1I GCGC 1 cut(s) 1039
HinfI GANTC 4 cut(s) 22, 599, 681, 1058
HphI GGTGA 4 cut(s) 94, 802, 1053, 1082
Hpy166II GTNNAC 3 cut(s) 102, 481, 561
Hpy188I TCNGA 6 cut(s) 27, 96, 201, 424, 680, 889
Hpy188III TCNNGA 4 cut(s) 350, 542, 989, 1099
Hpy8I GTNNAC 3 cut(s) 102, 481, 561
HpyAV CCTTC 5 cut(s) 285, 391, 787, 795, 927
HpyCH4III ACNGT 4 cut(s) 790, 869, 1078, 1087
HpyCH4V TGCA 9 cut(s) 17, 146, 167, 380, 430, 469, 481, 769, 912
HpyF10VI GCNNNNNNNGC 3 cut(s) 593, 622, 909
HpyF3I CTNAG 1 cut(s) 899
Hsp92II CATG 7 cut(s) 196, 295, 353, 469, 545, 688, 963
HspAI GCGC 1 cut(s) 1039
KflI GGGWCCC 1 cut(s) 249
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
LmnI GCTCC 3 cut(s) 400, 613, 664
MaeIII GTNAC 1 cut(s) 187
MalI GATC 7 cut(s) 203, 260, 273, 537, 798, 837, 846
MboI GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
MboII GAAGA 5 cut(s) 530, 545, 665, 826, 1114
MhlI GDGCHC 2 cut(s) 483, 669
MlsI TGGCCA 1 cut(s) 130
MluCI AATT 4 cut(s) 369, 726, 809, 1026
MluNI TGGCCA 1 cut(s) 130
Mox20I TGGCCA 1 cut(s) 130
Mph1103I ATGCAT 1 cut(s) 432
MroXI GAANNNNTTC 1 cut(s) 549
MscI TGGCCA 1 cut(s) 130
MseI TTAA 1 cut(s) 830
Msp20I TGGCCA 1 cut(s) 130
Mva1269I GAATGC 1 cut(s) 430
MwoI GCNNNNNNNGC 3 cut(s) 593, 622, 909
NcoI CCATGG 1 cut(s) 192
NdeII GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
NlaIII CATG 7 cut(s) 196, 295, 353, 469, 545, 688, 963
NlaIV GGNNCC 3 cut(s) 250, 251, 690
NsiI ATGCAT 1 cut(s) 432
NspI RCATGY 1 cut(s) 295
NspV TTCGAA 1 cut(s) 735
PagI TCATGA 2 cut(s) 349, 541
PctI GAATGC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 4 cut(s) 22, 599, 681, 1058
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 249
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 250, 251, 690
PspPI GGNCC 3 cut(s) 67, 116, 249
PspPPI RGGWCCY 1 cut(s) 249
RsaI GTAC 3 cut(s) 290, 978, 1048
RsaNI GTAC 3 cut(s) 289, 977, 1047
SacI GAGCTC 1 cut(s) 669
SaqAI TTAA 1 cut(s) 830
Sau3AI GATC 7 cut(s) 201, 258, 271, 535, 796, 835, 844
Sau96I GGNCC 3 cut(s) 67, 116, 249
SduI GDGCHC 2 cut(s) 483, 669
SfcI CTRYAG 1 cut(s) 948
SfuI TTCGAA 1 cut(s) 735
SinI GGWCC 2 cut(s) 116, 249
SmlI CTYRAG 2 cut(s) 746, 1097
SmoI CTYRAG 2 cut(s) 746, 1097
Sse9I AATT 4 cut(s) 369, 726, 809, 1026
SsiI CCGC 1 cut(s) 246
SstI GAGCTC 1 cut(s) 669
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 4 cut(s) 790, 869, 1078, 1087
TaqI TCGA 2 cut(s) 735, 799
TasI AATT 4 cut(s) 369, 726, 809, 1026
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 4 cut(s) 22, 599, 681, 1058
Tru1I TTAA 1 cut(s) 830
Tru9I TTAA 1 cut(s) 830
TscAI CASTG 3 cut(s) 874, 1092, 1128
TspDTI ATGAA 8 cut(s) 64, 338, 530, 558, 673, 728, 910, 1038
TspGWI ACGGA 1 cut(s) 677
TspRI CASTG 3 cut(s) 874, 1092, 1128
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 2 cut(s) 116, 249
XapI RAATTY 2 cut(s) 369, 1026
XceI RCATGY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 2 cut(s) 472, 699
XmnI GAANNNNTTC 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.