Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0013182

Physical Location & Seq

Genomic Coordinates Forward (+)

53942701 .. 53944263

Gene Structure 1563 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G027440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGGGTTTTTTCTCTGCAATGATTCTGAACTGGCTACATTTCTGGGATGTAGCACTTGCCTTGATAGGCCTGTTCATATTTAGTTGCTTGCTTCAGAGGTTCACCAACAAAAATGGTCCAATGTTATGGCCAGTTTTGGGGATTGCACCCTCCATATTTCTAAATGCACACACCATCTATGATTGGGCTACCATGGCTCTGATCAAAGCAGGGGGAACATTCCACTACAAAGGAATGTGGTTCGGCGGGTGCCACGGGATCATAACACTTGATCCTTCAAACATTGAGTACATGCTCAAAACAAGGTTCAGCAACTTCCCAAAAGGCAAGTATTACAGGGAGAGGTTTCATGATTTGCTTGGGGATGGAATTTTCAATGCAGACAATGAGTTGTGGAAGGAGCAGAGGAGGTTGGCAACTTCAGAAATGCATTCGGGTCGTTTTGTGGAGTATTCGTTTTTCTCCATGCAAGAGTTGGTGCACAAGAAGTTGTTGAAGTTGATGGAGAAAGTGGTAGTATCAGGGTGTAGCATTGATCTTCATGAAGTGCTTCTTCGGTTTACTTTTGACAACATTTGCTCGTTAGCTCTTGGTGCTGATTCAGGGTGTTTGGACCTTGATTTGCCTCAAAGTCCTTTTGTGAAAGCCTTTGAAGAAGCCACGGAGCTCACTATGTTCAGATTCATATTGCCACCTTTTGTGTGGAAGCCGATGAAGTTGTTTGGAATTGGGTTCGAAAAGAGGCTCAAGGAAGCAACTAAAATAGTGCATTATTTTGCTGAAAATACGGTCAAGGATCGAAGGGGAGAATTGAAGAGGCTCGGTAGCTTAAAAGATCGTTTTGATCTATTGTCAAGGCTTATGGACAGTGAAAAGCAACATTTTTCGGATAAGTTTCTACGCGATTTCTGCATAAGTTTCATTTTGGCTGGAAGGGACACAAGCTCTGTAGGATTGGCATGGTTTTTTTGGTTGGTACAAAAGCATCCTGAAGTGGAAAACAAAATCCTCAAAGAAATCAATGAAATTTTAGGCCAGCGCCAATGCACCAAAAAAGAATCACCAGATGGTGATACTGTTTTTACAATGGAAGAACTCAAGAAGATGGTCTATTTACAAGCAGTGTTGTCAGAATCTTTAAGGCTTTACCCACCAGTGCCAATTGACATCAAAGAGGTCGTAGAAGATGATGTGTTTCCTGATGGTAGTATCGTCAAAAAGGGTGCTCGAGTTCTCTACTGCATTTACTCCATGGCTCGAATGGAGTCGATTTGGGGACAGGATTGCTTGCAGTTCAAGCCTGAAAGATGGATTAACAATGATGGTGAGCTTGTCAATGAGAATCAATTCAAATATGCTGTCTTTAATGGGGGTCCAAGGTTTTGTTTGGGGAAGAAGTTTGCTTACATGCAGATGAAAGTGGTGGCTGCAGCCACTCTGCTCAGGTATGAAGTTAAGGTTGTTGAAGGTCAAAATGTTGTTCCAAAGATGACAACCACACTATACATGCAGAATGGGCTGCTGGTGAGTCTTAAGCCTAGGTTGGTGACTACTATTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000038 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009698 GO:0009699 GO:0009987 GO:0010345 GO:0012505 GO:0016053 GO:0019438 GO:0019748 GO:0019752 GO:0032787 GO:0042761 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046394 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04144 ko04217 map00073 map04144 map04217

Pfam Domains

Protein Families

p450

Protein Analysis

520

Amino Acids

59.97

Weight (kDa)

8.61

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	78 - 500	3.4e-78	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13140 AT1G13140 AT1G13150 AT1G24540 AT3G26125
fragaria_vesca	FvH4_4g25350
malus_domestica	MD13G1104800.v1.1
prunus_persica	Prupe.1G241700_v2.0.a1
pyrus_communis	pycom13g09100
rosa_chinensis	RchiOBHm_Chr4g0432631
rosa_laevigata	RLG00000006828
rosa_multiflora	Rmu_sc0004612.1_g000006
rosa_roxburghii	Rroxscaffold_5G00374100
rosa_rugosa	Rorug04G0260900.1
rosa_samantha	Rh4BG323500 Rh4CG338600 Rh4DG319100
rosa_wichuraiana	Rw4G027440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	249
AccII	CGCG	1 cut(s)	903
AciI	CCGC	1 cut(s)	246
AclWI	GGATC	3 cut(s)	266, 266, 804
AcoI	YGGCCR	1 cut(s)	128
AcsI	RAATTY	2 cut(s)	369, 1026
AcuI	CTGAAG	3 cut(s)	77, 405, 1011
AfaI	GTAC	2 cut(s)	290, 978
AfiI	CCNNNNNNNGG	1 cut(s)	137
AflII	CTTAAG	1 cut(s)	1532
AgsI	TTSAA	8 cut(s)	279, 376, 496, 653, 814, 1297, 1351, 1466
AluBI	AGCT	5 cut(s)	587, 667, 828, 945, 1330
AluI	AGCT	5 cut(s)	587, 667, 828, 945, 1330
Alw21I	GWGCWC	3 cut(s)	483, 669, 1228
Alw44I	GTGCAC	1 cut(s)	479
AlwI	GGATC	3 cut(s)	266, 266, 804
Ama87I	CYCGRG	1 cut(s)	1227
AoxI	GGCC	3 cut(s)	67, 128, 1033
ApaLI	GTGCAC	1 cut(s)	479
ApeKI	GCWGC	3 cut(s)	1427, 1430, 1519
ApoI	RAATTY	2 cut(s)	369, 1026
ArsI	GACNNNNNNTTYG	2 cut(s)	1344, 1376
Asp700I	GAANNNNTTC	2 cut(s)	549, 1346
AspA2I	CCTAGG	1 cut(s)	1538
AspLEI	GCGC	1 cut(s)	1041
AspS9I	GGNCC	3 cut(s)	116, 613, 1373
AsuHPI	GGTGA	6 cut(s)	94, 1053, 1082, 1337, 1537, 1558
AsuII	TTCGAA	1 cut(s)	735
AvaI	CYCGRG	1 cut(s)	1227
AvaII	GGWCC	3 cut(s)	116, 613, 1373
AvrII	CCTAGG	1 cut(s)	1538
BaeGI	GKGCMC	1 cut(s)	483
BalI	TGGCCA	1 cut(s)	130
BanI	GGYRCC	1 cut(s)	249
BanII	GRGCYC	1 cut(s)	669
BarI	GAAGNNNNNNTAC	2 cut(s)	1388, 1420
Bbv12I	GWGCWC	3 cut(s)	483, 669, 1228
BbvI	GCAGC	3 cut(s)	1414, 1442, 1506
BccI	CCATC	8 cut(s)	182, 359, 496, 1061, 1099, 1196, 1302, 1316
BcgI	CGANNNNNNTGC	2 cut(s)	419, 453
BclI	TGATCA	1 cut(s)	201
BfaI	CTAG	1 cut(s)	1539
BfmI	CTRYAG	2 cut(s)	948, 1428
BfoI	RGCGCY	1 cut(s)	1042
BfrI	CTTAAG	1 cut(s)	1532
BisI	GCNGC	3 cut(s)	1428, 1431, 1520
BlnI	CCTAGG	1 cut(s)	1538
BlsI	GCNGC	3 cut(s)	1429, 1432, 1521
Bme18I	GGWCC	3 cut(s)	116, 613, 1373
BmeT110I	CYCGRG	1 cut(s)	1227
BmgT120I	GGNCC	3 cut(s)	116, 613, 1373
BmiI	GGNNCC	2 cut(s)	251, 1374
BmsI	GCATC	1 cut(s)	994
Bpu10I	CCTNAGC	1 cut(s)	1442
Bpu14I	TTCGAA	1 cut(s)	735
BpuEI	CTTGAG	2 cut(s)	731, 1082
BsaJI	CCNNGG	6 cut(s)	192, 253, 660, 1251, 1376, 1538
BsaXI	ACNNNNNCTCC	2 cut(s)	440, 470
Bsc4I	CCNNNNNNNGG	1 cut(s)	137
Bse1I	ACTGG	3 cut(s)	35, 131, 1154
Bse3DI	GCAATG	1 cut(s)	24
BseDI	CCNNGG	6 cut(s)	192, 253, 660, 1251, 1376, 1538
BseGI	GGATG	3 cut(s)	52, 370, 985
BseLI	CCNNNNNNNGG	1 cut(s)	137
BseMI	GCAATG	1 cut(s)	24
BseMII	CTCAG	1 cut(s)	1456
BseNI	ACTGG	3 cut(s)	35, 131, 1154
BseRI	GAGGAG	1 cut(s)	421
BseSI	GKGCMC	1 cut(s)	483
BseXI	GCAGC	3 cut(s)	1414, 1442, 1506
Bsh1236I	CGCG	1 cut(s)	903
BshFI	GGCC	3 cut(s)	69, 130, 1035
BshNI	GGYRCC	1 cut(s)	249
BsiHKAI	GWGCWC	3 cut(s)	483, 669, 1228
BsiHKCI	CYCGRG	1 cut(s)	1227
BslFI	GGGAC	2 cut(s)	950, 1290
BslI	CCNNNNNNNGG	1 cut(s)	137
BsmFI	GGGAC	2 cut(s)	950, 1290
BsmI	GAATGC	1 cut(s)	430
BsnI	GGCC	3 cut(s)	69, 130, 1035
BsoBI	CYCGRG	1 cut(s)	1227
Bsp119I	TTCGAA	1 cut(s)	735
Bsp1286I	GDGCHC	3 cut(s)	483, 669, 1228
Bsp143I	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
Bsp19I	CCATGG	2 cut(s)	192, 1251
BspACI	CCGC	1 cut(s)	246
BspANI	GGCC	3 cut(s)	69, 130, 1035
BspCNI	CTCAG	1 cut(s)	1455
BspFNI	CGCG	1 cut(s)	903
BspHI	TCATGA	2 cut(s)	349, 541
BspLI	GGNNCC	2 cut(s)	251, 1374
BspMAI	CTGCAG	1 cut(s)	1432
BspPI	GGATC	3 cut(s)	266, 266, 804
BspT104I	TTCGAA	1 cut(s)	735
BspT107I	GGYRCC	1 cut(s)	249
BspTI	CTTAAG	1 cut(s)	1532
BsrDI	GCAATG	1 cut(s)	24
BsrI	ACTGG	3 cut(s)	35, 131, 1154
BssECI	CCNNGG	6 cut(s)	192, 253, 660, 1251, 1376, 1538
BssMI	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
BssT1I	CCWWGG	4 cut(s)	192, 1251, 1376, 1538
Bst4CI	ACNGT	3 cut(s)	790, 869, 1078
Bst6I	CTCTTC	1 cut(s)	809
BstAFI	CTTAAG	1 cut(s)	1532
BstBI	TTCGAA	1 cut(s)	735
BstC8I	GCNNGC	3 cut(s)	89, 1037, 1289
BstDEI	CTNAG	1 cut(s)	1442
BstDSI	CCRYGG	4 cut(s)	192, 253, 660, 1251
BstF5I	GGATG	3 cut(s)	52, 370, 985
BstFNI	CGCG	1 cut(s)	903
BstH2I	RGCGCY	1 cut(s)	1042
BstHHI	GCGC	1 cut(s)	1041
BstKTI	GATC	7 cut(s)	204, 261, 274, 538, 799, 838, 847
BstMBI	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
BstMWI	GCNNNNNNNGC	5 cut(s)	194, 593, 909, 1297, 1516
BstNSI	RCATGY	3 cut(s)	295, 1411, 1510
BstSFI	CTRYAG	2 cut(s)	948, 1428
BstSLI	GKGCMC	1 cut(s)	483
BstUI	CGCG	1 cut(s)	903
BstV1I	GCAGC	3 cut(s)	1414, 1442, 1506
BstXI	CCANNNNNNTGG	1 cut(s)	126
BsuRI	GGCC	3 cut(s)	69, 130, 1035
BtgI	CCRYGG	4 cut(s)	192, 253, 660, 1251
BtsCI	GGATG	3 cut(s)	52, 370, 985
BtsI	GCAGTG	1 cut(s)	1128
BtsIMutI	CAGTG	3 cut(s)	874, 1128, 1161
Cac8I	GCNNGC	3 cut(s)	89, 1037, 1289
CciI	TCATGA	2 cut(s)	349, 541
CfoI	GCGC	1 cut(s)	1041
Cfr13I	GGNCC	3 cut(s)	116, 613, 1373
Csp6I	GTAC	2 cut(s)	289, 977
CviAII	CATG	9 cut(s)	193, 292, 350, 466, 542, 960, 1252, 1408, 1507
CviQI	GTAC	2 cut(s)	289, 977
DdeI	CTNAG	1 cut(s)	1442
DpnI	GATC	7 cut(s)	203, 260, 273, 537, 798, 837, 846
DpnII	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
EaeI	YGGCCR	1 cut(s)	128
Eam1104I	CTCTTC	1 cut(s)	809
EarI	CTCTTC	1 cut(s)	809
Ecl136II	GAGCTC	1 cut(s)	667
Eco130I	CCWWGG	4 cut(s)	192, 1251, 1376, 1538
Eco147I	AGGCCT	1 cut(s)	69
Eco24I	GRGCYC	1 cut(s)	669
Eco47I	GGWCC	3 cut(s)	116, 613, 1373
Eco53kI	GAGCTC	1 cut(s)	667
Eco57I	CTGAAG	3 cut(s)	77, 405, 1011
Eco88I	CYCGRG	1 cut(s)	1227
EcoICRI	GAGCTC	1 cut(s)	667
EcoT14I	CCWWGG	4 cut(s)	192, 1251, 1376, 1538
EcoT22I	ATGCAT	1 cut(s)	432
EcoT38I	GRGCYC	1 cut(s)	669
ErhI	CCWWGG	4 cut(s)	192, 1251, 1376, 1538
FaeI	CATG	9 cut(s)	196, 295, 353, 469, 545, 963, 1255, 1411, 1510
FalI	AAGNNNNNCTT	2 cut(s)	537, 569
FaqI	GGGAC	2 cut(s)	950, 1290
FatI	CATG	9 cut(s)	192, 291, 349, 465, 541, 959, 1251, 1407, 1506
FauI	CCCGC	1 cut(s)	239
FbaI	TGATCA	1 cut(s)	201
Fnu4HI	GCNGC	3 cut(s)	1428, 1431, 1520
FokI	GGATG	3 cut(s)	59, 377, 972
FriOI	GRGCYC	1 cut(s)	669
Fsp4HI	GCNGC	3 cut(s)	1428, 1431, 1520
FspBI	CTAG	1 cut(s)	1539
GlaI	GCGC	1 cut(s)	1040
GluI	GCNGC	3 cut(s)	1428, 1431, 1520
HaeII	RGCGCY	1 cut(s)	1042
HaeIII	GGCC	3 cut(s)	69, 130, 1035
HhaI	GCGC	1 cut(s)	1041
Hin1II	CATG	9 cut(s)	196, 295, 353, 469, 545, 963, 1255, 1411, 1510
Hin6I	GCGC	1 cut(s)	1039
HinP1I	GCGC	1 cut(s)	1039
HinfI	GANTC	8 cut(s)	22, 599, 681, 1058, 1133, 1265, 1342, 1528
HphI	GGTGA	6 cut(s)	94, 1053, 1082, 1337, 1537, 1558
Hpy166II	GTNNAC	3 cut(s)	102, 481, 561
Hpy188I	TCNGA	7 cut(s)	27, 96, 201, 424, 680, 889, 1132
Hpy188III	TCNNGA	5 cut(s)	350, 542, 989, 1099, 1199
Hpy8I	GTNNAC	3 cut(s)	102, 481, 561
HpyAV	CCTTC	5 cut(s)	285, 391, 795, 927, 1460
HpyCH4III	ACNGT	3 cut(s)	790, 869, 1078
HpyF10VI	GCNNNNNNNGC	5 cut(s)	194, 593, 909, 1297, 1516
HpyF3I	CTNAG	1 cut(s)	1442
Hsp92II	CATG	9 cut(s)	196, 295, 353, 469, 545, 963, 1255, 1411, 1510
HspAI	GCGC	1 cut(s)	1039
Ksp22I	TGATCA	1 cut(s)	201
Kzo9I	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
LmnI	GCTCC	2 cut(s)	400, 664
Lsp1109I	GCAGC	3 cut(s)	1414, 1442, 1506
LweI	GCATC	1 cut(s)	994
MaeI	CTAG	1 cut(s)	1539
MaeIII	GTNAC	1 cut(s)	1546
MalI	GATC	7 cut(s)	203, 260, 273, 537, 798, 837, 846
MboI	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
MboII	GAAGA	8 cut(s)	530, 545, 665, 826, 1103, 1114, 1196, 1405
MfeI	CAATTG	1 cut(s)	1161
MhlI	GDGCHC	3 cut(s)	483, 669, 1228
MlsI	TGGCCA	1 cut(s)	130
MluCI	AATT	6 cut(s)	369, 726, 809, 1026, 1161, 1346
MluNI	TGGCCA	1 cut(s)	130
MlyI	GAGTC	2 cut(s)	1274, 1537
Mox20I	TGGCCA	1 cut(s)	130
Mph1103I	ATGCAT	1 cut(s)	432
MroXI	GAANNNNTTC	2 cut(s)	549, 1346
MscI	TGGCCA	1 cut(s)	130
MseI	TTAA	6 cut(s)	830, 1139, 1314, 1365, 1455, 1533
MslI	CAYNNNNRTG	1 cut(s)	1412
Msp20I	TGGCCA	1 cut(s)	130
MspCI	CTTAAG	1 cut(s)	1532
MunI	CAATTG	1 cut(s)	1161
Mva1269I	GAATGC	1 cut(s)	430
MvnI	CGCG	1 cut(s)	903
MwoI	GCNNNNNNNGC	5 cut(s)	194, 593, 909, 1297, 1516
NcoI	CCATGG	2 cut(s)	192, 1251
NdeII	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
NlaIII	CATG	9 cut(s)	196, 295, 353, 469, 545, 963, 1255, 1411, 1510
NlaIV	GGNNCC	2 cut(s)	251, 1374
NmuCI	GTSAC	1 cut(s)	1546
NsiI	ATGCAT	1 cut(s)	432
NspI	RCATGY	3 cut(s)	295, 1411, 1510
NspV	TTCGAA	1 cut(s)	735
PaeR7I	CTCGAG	1 cut(s)	1227
PagI	TCATGA	2 cut(s)	349, 541
PceI	AGGCCT	1 cut(s)	69
PctI	GAATGC	1 cut(s)	430
PdmI	GAANNNNTTC	2 cut(s)	549, 1346
PfeI	GAWTC	6 cut(s)	22, 599, 681, 1058, 1133, 1342
PkrI	GCNGC	3 cut(s)	1429, 1432, 1521
PleI	GAGTC	2 cut(s)	1273, 1536
PpsI	GAGTC	2 cut(s)	1273, 1536
Psp124BI	GAGCTC	1 cut(s)	669
PspN4I	GGNNCC	2 cut(s)	251, 1374
PspPI	GGNCC	3 cut(s)	116, 613, 1373
PspXI	VCTCGAGB	1 cut(s)	1227
PstI	CTGCAG	1 cut(s)	1432
RsaI	GTAC	2 cut(s)	290, 978
RsaNI	GTAC	2 cut(s)	289, 977
RseI	CAYNNNNRTG	1 cut(s)	1412
SacI	GAGCTC	1 cut(s)	669
SaqAI	TTAA	6 cut(s)	830, 1139, 1314, 1365, 1455, 1533
SatI	GCNGC	3 cut(s)	1428, 1431, 1520
Sau3AI	GATC	7 cut(s)	201, 258, 271, 535, 796, 835, 844
Sau96I	GGNCC	3 cut(s)	116, 613, 1373
SchI	GAGTC	2 cut(s)	1274, 1537
SduI	GDGCHC	3 cut(s)	483, 669, 1228
SfaNI	GCATC	1 cut(s)	994
SfcI	CTRYAG	2 cut(s)	948, 1428
Sfr274I	CTCGAG	1 cut(s)	1227
SfuI	TTCGAA	1 cut(s)	735
SinI	GGWCC	3 cut(s)	116, 613, 1373
SlaI	CTCGAG	1 cut(s)	1227
SmiMI	CAYNNNNRTG	1 cut(s)	1412
SmlI	CTYRAG	4 cut(s)	746, 1097, 1227, 1532
SmoI	CTYRAG	4 cut(s)	746, 1097, 1227, 1532
Sse9I	AATT	6 cut(s)	369, 726, 809, 1026, 1161, 1346
SseBI	AGGCCT	1 cut(s)	69
SsiI	CCGC	1 cut(s)	246
SspMI	CTAG	1 cut(s)	1539
SstI	GAGCTC	1 cut(s)	669
StuI	AGGCCT	1 cut(s)	69
StyI	CCWWGG	4 cut(s)	192, 1251, 1376, 1538
TaaI	ACNGT	3 cut(s)	790, 869, 1078
TaqI	TCGA	5 cut(s)	735, 799, 1228, 1258, 1268
TasI	AATT	6 cut(s)	369, 726, 809, 1026, 1161, 1346
TatI	WGTACW	1 cut(s)	288
TfiI	GAWTC	6 cut(s)	22, 599, 681, 1058, 1133, 1342
Tru1I	TTAA	6 cut(s)	830, 1139, 1314, 1365, 1455, 1533
Tru9I	TTAA	6 cut(s)	830, 1139, 1314, 1365, 1455, 1533
TscAI	CASTG	3 cut(s)	874, 1128, 1161
TseFI	GTSAC	1 cut(s)	1546
TseI	GCWGC	3 cut(s)	1427, 1430, 1519
Tsp45I	GTSAC	1 cut(s)	1546
TspGWI	ACGGA	1 cut(s)	677
TspRI	CASTG	3 cut(s)	874, 1128, 1161
Vha464I	CTTAAG	1 cut(s)	1532
VneI	GTGCAC	1 cut(s)	479
VpaK11BI	GGWCC	3 cut(s)	116, 613, 1373
XapI	RAATTY	2 cut(s)	369, 1026
XceI	RCATGY	3 cut(s)	295, 1411, 1510
XcmI	CCANNNNNNNNNTGG	3 cut(s)	472, 699, 1258
XhoI	CTCGAG	1 cut(s)	1227
XmaJI	CCTAGG	1 cut(s)	1538
XmnI	GAANNNNTTC	2 cut(s)	549, 1346
XspI	CTAG	1 cut(s)	1539
Zsp2I	ATGCAT	1 cut(s)	432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.