AT1G14700

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007589
Physical Location & Seq
Forward (+)
5058583 .. 5061140
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14700.1

Sequence Viewer

Length: 1101 bp
ATGACCTACATATATAGAGACACAAAGATCACAACAAAATCAACCATTCCTTTCCTTATCTTCTTCCTCTTTTGCTTTTCTAATCTCTCAATGGCTACCTTAAAACACAAACCTGTAAATTTGGTATTTTACGTTTATAATTTGATTATTATATTCTCCTCCCATAGTTCCACGGCGGAGCTCCGCAGGCTACTGCAGCCATCAAAAACCGATGGTACAGTGAGCTTTCTCGTTATCGGAGACTGGGGAAGAAGAGGCTCCTATAATCAGTCCCAAGTAGCTCTTCAGATGGGAGAAATCGGAGAGAAATTAGATATCGATTTTGTGATTTCTACTGGAGATAATTTTTACGACAATGGTTTAACCAGCTTGCACGATCCTCTATTTCAAGATTCTTTTACCAACATTTATACCGCTCCGAGCTTGCAGAAACCTTGGTATAGTGTTTTAGGAAATCATGATTATAGAGGTGACGTCAGGGCACAACTTAGCCCTATGCTCAGAGCTCTGGACAACAGATGGGTTTGCATGAGATCTTTCATTGTAAATGCTGAGATCGTCGATTTGTTCTTCGTCGACACAACTCCGTTCGTCGATAAATACTTTATTCAACCAAATAAGCATGTTTATGACTGGAGCGGTGTATTACCTAGGCAAACATATCTGAATAATCTCTTAAAGGAACTCGACGTGGCGTTGAGAGAGTCAGTCGCAAAGTGGAAAATAGTGATTGGTCACCATACGATCAAAAGCGCAGGCCACCACGGGAACACCATTGAGTTGGAGAAACACCTTTTACCTATTCTCCAAGCAAATGAAGTGGATCTCTACGTAAACGGACATGATCATTGCTTAGAGCACATAAGCAGCGTGGACAGTAACATACAATTTATGACAAGTGGAGGTGGATCCAAGGCCTGGAAAGGAGGTGACGTGAACTACGTTGAACCAGAAGAGATGAGGTTTTACTACGACGGACAAGGTTTCATGTCGGTTCACGTTTCAGAAGCTGAACTGCGTGTAGTCTTTTATGATGTTTTTGGACACGTTCTGCATCATTGGAAGAAGACTTACAAGGAGGCGCTTTATTTCGCTTCTTGA

Protein Analysis

366

Amino Acids

42.05

Weight (kDa)

6.27

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 75 - 284 4.1e-20 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
AatII GACGTC 1 cut(s) 477
AccB7I CCANNNNNTGG 1 cut(s) 918
AccBSI CCGCTC 2 cut(s) 416, 639
AccI GTMKAC 1 cut(s) 576
AciI CCGC 4 cut(s) 176, 184, 414, 639
AclWI GGATC 4 cut(s) 371, 831, 903, 916
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 1 cut(s) 918
AflIII ACRYGT 1 cut(s) 1045
AgsI TTSAA 3 cut(s) 389, 611, 947
AjiI CACGTC 2 cut(s) 691, 934
AjnI CCWGG 1 cut(s) 917
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
AluBI AGCT 7 cut(s) 181, 225, 281, 369, 423, 506, 1010
AluI AGCT 7 cut(s) 181, 225, 281, 369, 423, 506, 1010
Alw21I GWGCWC 3 cut(s) 183, 508, 861
Alw26I GTCTC 2 cut(s) 12, 234
AlwI GGATC 4 cut(s) 371, 831, 903, 916
AlwNI CAGNNNCTG 1 cut(s) 1010
AoxI GGCC 2 cut(s) 757, 915
ApeKI GCWGC 2 cut(s) 196, 867
ApoI RAATTY 1 cut(s) 118
AspA2I CCTAGG 1 cut(s) 650
AspLEI GCGC 2 cut(s) 755, 1084
AsuHPI GGTGA 3 cut(s) 482, 728, 941
AvrII CCTAGG 1 cut(s) 650
BaeGI GKGCMC 1 cut(s) 484
BamHI GGATCC 1 cut(s) 908
BanII GRGCYC 2 cut(s) 183, 508
BarI GAAGNNNNNNTAC 2 cut(s) 1055, 1087
BbsI GAAGAC 1 cut(s) 1073
Bbv12I GWGCWC 3 cut(s) 183, 508, 861
BbvI GCAGC 2 cut(s) 208, 879
BccI CCATC 4 cut(s) 206, 208, 283, 513
BceAI ACGGC 1 cut(s) 189
BciT130I CCWGG 1 cut(s) 919
BclI TGATCA 1 cut(s) 844
BcoDI GTCTC 2 cut(s) 12, 234
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 1085
BglII AGATCT 1 cut(s) 533
BisI GCNGC 2 cut(s) 197, 868
BlnI CCTAGG 1 cut(s) 650
BlsI GCNGC 2 cut(s) 198, 869
Bme1390I CCNGG 1 cut(s) 919
BmgBI CACGTC 2 cut(s) 691, 934
BmiI GGNNCC 2 cut(s) 259, 910
BmrFI CCNGG 1 cut(s) 919
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 1063
BmuI ACTGGG 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 1073
BpmI CTGGAG 2 cut(s) 357, 655
Bsa29I ATCGAT 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 832
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 5 cut(s) 171, 434, 650, 763, 912
BsaXI ACNNNNNCTCC 4 cut(s) 789, 819, 918, 948
Bsc4I CCNNNNNNNGG 1 cut(s) 918
Bse1I ACTGG 3 cut(s) 248, 340, 638
Bse3DI GCAATG 1 cut(s) 847
BseBI CCWGG 1 cut(s) 919
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 5 cut(s) 171, 434, 650, 763, 912
BseLI CCNNNNNNNGG 1 cut(s) 918
BseMI GCAATG 1 cut(s) 847
BseMII CTCAG 2 cut(s) 514, 543
BseNI ACTGG 3 cut(s) 248, 340, 638
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 2 cut(s) 208, 879
BshFI GGCC 2 cut(s) 759, 917
BshVI ATCGAT 1 cut(s) 318
BsiHKAI GWGCWC 3 cut(s) 183, 508, 861
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 1 cut(s) 918
BsmAI GTCTC 2 cut(s) 12, 234
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 2 cut(s) 759, 917
Bsp1286I GDGCHC 4 cut(s) 183, 484, 508, 861
Bsp143I GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
BspACI CCGC 4 cut(s) 176, 184, 414, 639
BspANI GGCC 2 cut(s) 759, 917
BspCNI CTCAG 2 cut(s) 513, 544
BspDI ATCGAT 1 cut(s) 318
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 259, 910
BspMAI CTGCAG 1 cut(s) 198
BspPI GGATC 4 cut(s) 371, 831, 903, 916
BspQI GCTCTTC 1 cut(s) 288
BsrBI CCGCTC 2 cut(s) 416, 639
BsrDI GCAATG 1 cut(s) 847
BsrI ACTGG 3 cut(s) 248, 340, 638
BssECI CCNNGG 5 cut(s) 171, 434, 650, 763, 912
BssMI GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 3 cut(s) 434, 650, 912
Bst2UI CCWGG 1 cut(s) 919
Bst4CI ACNGT 2 cut(s) 220, 878
Bst6I CTCTTC 3 cut(s) 247, 288, 948
BstACI GRCGYC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 832
BstC8I GCNNGC 4 cut(s) 188, 371, 425, 757
BstDEI CTNAG 4 cut(s) 488, 500, 552, 853
BstDSI CCRYGG 2 cut(s) 171, 763
BstEII GGTNACC 1 cut(s) 734
BstH2I RGCGCY 1 cut(s) 1085
BstHHI GCGC 2 cut(s) 755, 1084
BstKTI GATC 8 cut(s) 30, 379, 536, 558, 747, 826, 847, 911
BstMAI GTCTC 2 cut(s) 12, 234
BstMBI GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
BstMWI GCNNNNNNNGC 2 cut(s) 187, 196
BstNI CCWGG 1 cut(s) 919
BstNSI RCATGY 1 cut(s) 626
BstPI GGTNACC 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 917
BstSFI CTRYAG 1 cut(s) 194
BstSLI GKGCMC 1 cut(s) 484
BstSNI TACGTA 1 cut(s) 832
BstV1I GCAGC 2 cut(s) 208, 879
BstV2I GAAGAC 1 cut(s) 1073
BstX2I RGATCY 3 cut(s) 533, 823, 908
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 3 cut(s) 533, 823, 908
Bsu15I ATCGAT 1 cut(s) 318
BsuRI GGCC 2 cut(s) 759, 917
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 2 cut(s) 171, 763
BtrI CACGTC 2 cut(s) 691, 934
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 4 cut(s) 188, 371, 425, 757
CaiI CAGNNNCTG 1 cut(s) 1010
CciI TCATGA 1 cut(s) 457
CfoI GCGC 2 cut(s) 755, 1084
ClaI ATCGAT 1 cut(s) 318
Csp6I GTAC 1 cut(s) 216
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 5 cut(s) 458, 529, 623, 842, 988
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 4 cut(s) 488, 500, 552, 853
DpnI GATC 8 cut(s) 29, 378, 535, 557, 746, 825, 846, 910
DpnII GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
Eam1104I CTCTTC 3 cut(s) 247, 288, 948
EarI CTCTTC 3 cut(s) 247, 288, 948
EciI GGCGGA 1 cut(s) 191
Ecl136II GAGCTC 2 cut(s) 181, 506
Eco105I TACGTA 1 cut(s) 832
Eco130I CCWWGG 3 cut(s) 434, 650, 912
Eco147I AGGCCT 1 cut(s) 917
Eco24I GRGCYC 2 cut(s) 183, 508
Eco32I GATATC 1 cut(s) 316
Eco53kI GAGCTC 2 cut(s) 181, 506
Eco57I CTGAAG 1 cut(s) 269
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 2 cut(s) 181, 506
EcoO65I GGTNACC 1 cut(s) 734
EcoRII CCWGG 1 cut(s) 917
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 434, 650, 912
EcoT38I GRGCYC 2 cut(s) 183, 508
ErhI CCWWGG 3 cut(s) 434, 650, 912
FaeI CATG 5 cut(s) 461, 532, 626, 845, 991
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 1 cut(s) 256
FatI CATG 5 cut(s) 457, 528, 622, 841, 987
FbaI TGATCA 1 cut(s) 844
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 197, 868
FriOI GRGCYC 2 cut(s) 183, 508
Fsp4HI GCNGC 2 cut(s) 197, 868
FspBI CTAG 1 cut(s) 651
GlaI GCGC 2 cut(s) 754, 1083
GluI GCNGC 2 cut(s) 197, 868
GsuI CTGGAG 2 cut(s) 357, 655
HaeII RGCGCY 1 cut(s) 1085
HaeIII GGCC 2 cut(s) 759, 917
HhaI GCGC 2 cut(s) 755, 1084
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 5 cut(s) 461, 532, 626, 845, 991
Hin6I GCGC 2 cut(s) 753, 1082
HinP1I GCGC 2 cut(s) 753, 1082
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HinfI GANTC 2 cut(s) 392, 704
HphI GGTGA 3 cut(s) 482, 728, 941
Hpy166II GTNNAC 5 cut(s) 577, 835, 874, 937, 997
Hpy188I TCNGA 7 cut(s) 239, 288, 302, 420, 503, 666, 1006
Hpy188III TCNNGA 4 cut(s) 389, 458, 509, 1098
Hpy8I GTNNAC 5 cut(s) 577, 835, 874, 937, 997
Hpy99I CGWCG 5 cut(s) 563, 578, 596, 692, 977
HpyCH4III ACNGT 2 cut(s) 220, 878
HpyCH4IV ACGT 8 cut(s) 132, 474, 690, 831, 933, 942, 999, 1047
HpyCH4V TGCA 5 cut(s) 196, 373, 427, 528, 1054
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 196
HpyF3I CTNAG 4 cut(s) 488, 500, 552, 853
HpySE526I ACGT 8 cut(s) 132, 474, 690, 831, 933, 942, 999, 1047
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 5 cut(s) 461, 532, 626, 845, 991
HspAI GCGC 2 cut(s) 753, 1082
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 5 cut(s) 178, 186, 263, 421, 636
Lsp1109I GCAGC 2 cut(s) 208, 879
LweI GCATC 1 cut(s) 1063
MaeI CTAG 1 cut(s) 651
MaeII ACGT 8 cut(s) 132, 474, 690, 831, 933, 942, 999, 1047
MaeIII GTNAC 4 cut(s) 470, 734, 878, 929
MalI GATC 8 cut(s) 29, 378, 535, 557, 746, 825, 846, 910
MbiI CCGCTC 2 cut(s) 416, 639
MboI GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
MboII GAAGA 9 cut(s) 52, 55, 261, 264, 275, 562, 965, 1075, 1078
MflI RGATCY 3 cut(s) 533, 823, 908
MhlI GDGCHC 4 cut(s) 183, 484, 508, 861
MluCI AATT 5 cut(s) 118, 139, 308, 343, 887
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 762
MnlI CCTC 9 cut(s) 77, 169, 248, 390, 461, 896, 920, 954, 1072
MseI TTAA 3 cut(s) 101, 362, 677
MslI CAYNNNNRTG 1 cut(s) 627
MspR9I CCNGG 1 cut(s) 919
MvaI CCWGG 1 cut(s) 919
MwoI GCNNNNNNNGC 2 cut(s) 187, 196
NdeII GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
NlaIII CATG 5 cut(s) 461, 532, 626, 845, 991
NlaIV GGNNCC 2 cut(s) 259, 910
NmuCI GTSAC 3 cut(s) 470, 734, 929
NspI RCATGY 1 cut(s) 626
PagI TCATGA 1 cut(s) 457
PceI AGGCCT 1 cut(s) 917
PciSI GCTCTTC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 939
PfeI GAWTC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 918
PkrI GCNGC 2 cut(s) 198, 869
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
Ppu21I YACGTR 1 cut(s) 832
PsiI TTATAA 1 cut(s) 138
Psp124BI GAGCTC 2 cut(s) 183, 508
Psp6I CCWGG 1 cut(s) 917
PspEI GGTNACC 1 cut(s) 734
PspGI CCWGG 1 cut(s) 917
PspN4I GGNNCC 2 cut(s) 259, 910
PstI CTGCAG 1 cut(s) 198
PstNI CAGNNNCTG 1 cut(s) 1010
PsuI RGATCY 3 cut(s) 533, 823, 908
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 627
SacI GAGCTC 2 cut(s) 183, 508
SalI GTCGAC 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 3 cut(s) 101, 362, 677
SatI GCNGC 2 cut(s) 197, 868
Sau3AI GATC 8 cut(s) 27, 376, 533, 555, 744, 823, 844, 908
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 1 cut(s) 919
SduI GDGCHC 4 cut(s) 183, 484, 508, 861
SfaNI GCATC 1 cut(s) 1063
SfcI CTRYAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 627
SnaBI TACGTA 1 cut(s) 832
Sse9I AATT 5 cut(s) 118, 139, 308, 343, 887
SseBI AGGCCT 1 cut(s) 917
SsiI CCGC 4 cut(s) 176, 184, 414, 639
SspMI CTAG 1 cut(s) 651
SstI GAGCTC 2 cut(s) 183, 508
StuI AGGCCT 1 cut(s) 917
StyD4I CCNGG 1 cut(s) 917
StyI CCWWGG 3 cut(s) 434, 650, 912
TaaI ACNGT 2 cut(s) 220, 878
TaiI ACGT 8 cut(s) 135, 477, 693, 834, 936, 945, 1002, 1050
TaqI TCGA 5 cut(s) 318, 561, 576, 594, 687
TasI AATT 5 cut(s) 118, 139, 308, 343, 887
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 3 cut(s) 101, 362, 677
Tru9I TTAA 3 cut(s) 101, 362, 677
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 3 cut(s) 470, 734, 929
TseI GCWGC 2 cut(s) 196, 867
Tsp45I GTSAC 3 cut(s) 470, 734, 929
TspDTI ATGAA 3 cut(s) 529, 831, 976
TspGWI ACGGA 3 cut(s) 576, 852, 990
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 1 cut(s) 918
XapI RAATTY 1 cut(s) 118
XceI RCATGY 1 cut(s) 626
XmaJI CCTAGG 1 cut(s) 650
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 1 cut(s) 651
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.