MD06G1233400.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007589
Physical Location & Seq
Reverse (-)
36460089 .. 36461866
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1233400.v1.1.491

Sequence Viewer

Length: 618 bp
ATGTCCTTGGCCATGGGGTTACTGTGGGTGGTTGTTTGCATGAATATTGTTCTGTTGAGCTTTGTTTCTGTGTCTACGGCGGAGCTTCAGCGATTTGAACACCCTGTGAAACCTGATGGCTCACTCAGCTTTTTGGCGGTTGGTGACTGGGGTAGAAGGGGTGGTTACAATCAATCTCAAGTTGCTTTTCAGATGGGGAAGATTGGAGCCAAATTAAACGTAGACTTCGTCATCTCCACGGGTGACAACTTTTACAACAGGGGATTGACCAGCGTCAACGACCCTGCATTTCTAAAGTCGTTTACTGAAATCTACACTGCTCAGAGCCTGCAAAAGCAATGGTATCTTGTTCTCGGCAACCATGACTACCGGGGAAACGCTTTGGCCCAGTTGAGTCCGGTCCTTAGGAAGATGGACAGCCGGTGGCTTTGCCTCAGGTCCTTTCTTGTCAACACACAAGTTGCAGATTTCTTCTTCATTGACACCAACCCTTTCGTGGACAAGTACTTTTTGAGGCAAAAGTACTACAAGCATGACTGGAGAGGTGTTCTTCCTAGGAAGGAGTACATCTCCAACCTCCTGAAGGATCTAGATTTTGCATTAAGGAAATTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko04142 ko04380 ko05323 map00740 map01100 map04142 map04380 map05323
Pfam Domains
Protein Families
Metallophos

Protein Analysis

206

Amino Acids

23.65

Weight (kDa)

9.44

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 43 - 159 6.9e-11 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 74, 222
AciI CCGC 2 cut(s) 80, 137
AclWI GGATC 1 cut(s) 594
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 2 cut(s) 71, 602
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 3 cut(s) 506, 524, 566
AfiI CCNNNNNNNGG 2 cut(s) 496, 583
AgsI TTSAA 1 cut(s) 98
AluBI AGCT 3 cut(s) 60, 85, 129
AluI AGCT 3 cut(s) 60, 85, 129
AlwI GGATC 1 cut(s) 594
AlwNI CAGNNNCTG 1 cut(s) 328
AoxI GGCC 2 cut(s) 9, 384
ApoI RAATTY 1 cut(s) 608
AspA2I CCTAGG 1 cut(s) 554
AspS9I GGNCC 3 cut(s) 385, 400, 438
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 155, 254
AvaII GGWCC 2 cut(s) 400, 438
AvrII CCTAGG 1 cut(s) 554
AxyI CCTNAGG 2 cut(s) 404, 434
BalI TGGCCA 1 cut(s) 11
BccI CCATC 3 cut(s) 110, 187, 406
BceAI ACGGC 1 cut(s) 93
BcnI CCSGG 1 cut(s) 371
BfaI CTAG 2 cut(s) 555, 590
BlnI CCTAGG 1 cut(s) 554
BmcAI AGTACT 2 cut(s) 506, 524
Bme1390I CCNGG 1 cut(s) 371
Bme18I GGWCC 2 cut(s) 400, 438
BmgT120I GGNCC 3 cut(s) 385, 400, 438
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 1 cut(s) 371
BmrI ACTGGG 2 cut(s) 157, 382
BmuI ACTGGG 2 cut(s) 157, 382
BoxI GACNNNNGTC 1 cut(s) 272
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 371
BsaJI CCNNGG 5 cut(s) 6, 12, 237, 370, 554
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 2 cut(s) 496, 583
Bse118I RCCGGY 1 cut(s) 420
Bse1I ACTGG 3 cut(s) 152, 388, 542
Bse21I CCTNAGG 2 cut(s) 404, 434
Bse3DI GCAATG 1 cut(s) 344
BseDI CCNNGG 5 cut(s) 6, 12, 237, 370, 554
BseLI CCNNNNNNNGG 2 cut(s) 496, 583
BseMI GCAATG 1 cut(s) 344
BseMII CTCAG 3 cut(s) 139, 335, 448
BseNI ACTGG 3 cut(s) 152, 388, 542
BshFI GGCC 2 cut(s) 11, 386
BsiSI CCGG 3 cut(s) 370, 398, 421
BslI CCNNNNNNNGG 2 cut(s) 496, 583
BsnI GGCC 2 cut(s) 11, 386
Bsp143I GATC 1 cut(s) 586
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 2 cut(s) 80, 137
BspANI GGCC 2 cut(s) 11, 386
BspCNI CTCAG 3 cut(s) 138, 334, 447
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 1 cut(s) 594
BsrDI GCAATG 1 cut(s) 344
BsrFI RCCGGY 1 cut(s) 420
BsrI ACTGG 3 cut(s) 152, 388, 542
BssAI RCCGGY 1 cut(s) 420
BssECI CCNNGG 5 cut(s) 6, 12, 237, 370, 554
BssMI GATC 1 cut(s) 586
BssT1I CCWWGG 3 cut(s) 6, 12, 554
Bst4CI ACNGT 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 4 cut(s) 125, 321, 404, 434
BstDSI CCRYGG 2 cut(s) 12, 237
BstENI CCTNNNNNAGG 1 cut(s) 581
BstKTI GATC 1 cut(s) 589
BstMBI GATC 1 cut(s) 586
BstMWI GCNNNNNNNGC 1 cut(s) 126
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
Bsu36I CCTNAGG 2 cut(s) 404, 434
BsuRI GGCC 2 cut(s) 11, 386
BtgI CCRYGG 2 cut(s) 12, 237
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 329
CaiI CAGNNNCTG 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 420
Cfr13I GGNCC 3 cut(s) 385, 400, 438
CseI GACGC 1 cut(s) 262
Csp6I GTAC 3 cut(s) 505, 523, 565
CviAII CATG 4 cut(s) 13, 40, 362, 533
CviQI GTAC 3 cut(s) 505, 523, 565
DdeI CTNAG 4 cut(s) 125, 321, 404, 434
DpnI GATC 1 cut(s) 588
DpnII GATC 1 cut(s) 586
DraIII CACNNNGTG 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 9
EciI GGCGGA 1 cut(s) 95
Eco130I CCWWGG 3 cut(s) 6, 12, 554
Eco47I GGWCC 2 cut(s) 400, 438
Eco57I CTGAAG 2 cut(s) 71, 602
Eco81I CCTNAGG 2 cut(s) 404, 434
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 438
EcoT14I CCWWGG 3 cut(s) 6, 12, 554
ErhI CCWWGG 3 cut(s) 6, 12, 554
FaeI CATG 4 cut(s) 16, 43, 365, 536
FaiI YATR 4 cut(s) 14, 41, 363, 534
FatI CATG 4 cut(s) 12, 39, 361, 532
FblI GTMKAC 2 cut(s) 74, 222
FspBI CTAG 2 cut(s) 555, 590
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 2 cut(s) 11, 386
HapII CCGG 3 cut(s) 370, 398, 421
HgaI GACGC 1 cut(s) 262
Hin1II CATG 4 cut(s) 16, 43, 365, 536
HincII GTYRAC 2 cut(s) 277, 451
HindII GTYRAC 2 cut(s) 277, 451
HinfI GANTC 1 cut(s) 394
HpaII CCGG 3 cut(s) 370, 398, 421
HphI GGTGA 2 cut(s) 155, 254
Hpy166II GTNNAC 6 cut(s) 75, 223, 277, 303, 451, 499
Hpy188I TCNGA 2 cut(s) 192, 324
Hpy188III TCNNGA 2 cut(s) 580, 590
Hpy8I GTNNAC 6 cut(s) 75, 223, 277, 303, 451, 499
HpyAV CCTTC 3 cut(s) 150, 553, 577
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 5 cut(s) 39, 287, 331, 464, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 126
HpyF3I CTNAG 4 cut(s) 125, 321, 404, 434
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 4 cut(s) 16, 43, 365, 536
Kzo9I GATC 1 cut(s) 586
LmnI GCTCC 2 cut(s) 82, 206
MaeI CTAG 2 cut(s) 555, 590
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 4 cut(s) 18, 143, 164, 242
MalI GATC 1 cut(s) 588
MboI GATC 1 cut(s) 586
MboII GAAGA 5 cut(s) 211, 421, 463, 466, 542
MflI RGATCY 1 cut(s) 586
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 2 cut(s) 212, 608
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 597
MnlI CCTC 4 cut(s) 443, 507, 536, 587
Mox20I TGGCCA 1 cut(s) 11
MscI TGGCCA 1 cut(s) 11
MseI TTAA 2 cut(s) 215, 602
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 3 cut(s) 370, 398, 421
MspR9I CCNGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 126
NciI CCSGG 1 cut(s) 371
NcoI CCATGG 1 cut(s) 12
NdeII GATC 1 cut(s) 586
NlaIII CATG 4 cut(s) 16, 43, 365, 536
NlaIV GGNNCC 1 cut(s) 208
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 2 cut(s) 143, 242
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PflFI GACNNNGTC 1 cut(s) 227
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
PpuMI RGGWCCY 1 cut(s) 438
PshAI GACNNNNGTC 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 3 cut(s) 385, 400, 438
PspPPI RGGWCCY 1 cut(s) 438
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 586
PsyI GACNNNGTC 1 cut(s) 227
RsaI GTAC 3 cut(s) 506, 524, 566
RsaNI GTAC 3 cut(s) 505, 523, 565
SaqAI TTAA 2 cut(s) 215, 602
Sau3AI GATC 1 cut(s) 586
Sau96I GGNCC 3 cut(s) 385, 400, 438
ScaI AGTACT 2 cut(s) 506, 524
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 371
SetI ASST 8 cut(s) 62, 87, 115, 131, 222, 440, 547, 579
SinI GGWCC 2 cut(s) 400, 438
SmlI CTYRAG 1 cut(s) 177
SmoI CTYRAG 1 cut(s) 177
Sse9I AATT 2 cut(s) 212, 608
SsiI CCGC 2 cut(s) 80, 137
SspI AATATT 1 cut(s) 46
SspMI CTAG 2 cut(s) 555, 590
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 3 cut(s) 6, 12, 554
TaaI ACNGT 1 cut(s) 24
TaiI ACGT 1 cut(s) 222
TasI AATT 2 cut(s) 212, 608
TatI WGTACW 3 cut(s) 504, 522, 564
Tru1I TTAA 2 cut(s) 215, 602
Tru9I TTAA 2 cut(s) 215, 602
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 2 cut(s) 143, 242
Tsp45I GTSAC 2 cut(s) 143, 242
TspDTI ATGAA 2 cut(s) 56, 466
TspRI CASTG 1 cut(s) 322
Tth111I GACNNNGTC 1 cut(s) 227
VpaK11BI GGWCC 2 cut(s) 400, 438
XagI CCTNNNNNAGG 1 cut(s) 581
XapI RAATTY 1 cut(s) 608
XbaI TCTAGA 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmaJI CCTAGG 1 cut(s) 554
XmiI GTMKAC 2 cut(s) 74, 222
XspI CTAG 2 cut(s) 555, 590
ZrmI AGTACT 2 cut(s) 506, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.