Rw7G000800

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007589
Physical Location & Seq
Forward (+)
653305 .. 655222
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000800.1

Sequence Viewer

Length: 993 bp
ATGGGCGTGTACGTCGTCGTAATGAGGTGGTTGCTCTGGTTGTGGGTGGTTTGCATGTCAGTGTCAGCTGAGCTTCAGCGGTTTGAGCACCCCGTCGTCAAAACCGATGACTCCCTGAGCTTTCTGGCCGTCGGAGATTGGGGACGAAGAGGCGCTTACAATCAATCTCTAATCGCTCATCAGATGGGAATAATCGGAGAGAAATTAAACATAGACTTTGTGGTTTCAACTGGGGATAACTTTTACGAGAGGGGATTGACCAGCGTAAACGACCCCAAGTATCCCGAGTCATTTACTCAAATCTACACTGCTCAGAGCCTCCAAAAACAATGGTACATTGTACTTGGGAACCATGACTACAGGGGAAATGCTTTGGCACAGCTGAGTCCAGTTCTTCGGAAGATGGACAGCCGGTGGCTTTGCCTGAGGTCATTTCTCGTCAACACGAAAATTGCAGACTTCTTCTTCATCGACACCAATCCATTTGTGGACAAGTACTGGTCCAACCCAAAGCACTACAAGCACGATTGGAGAGGTGTCGTTCCTAGGCAGCGCTACCTCTCTACCCTCTTGAAGGATCTGGATTTTGCATTGAGAAATTCGACTGCAAACTGGAAGATTGTCGTTGCACATCATACAATCAGAAGCGTAGGGCATCACGGGGATACCCAGGAAATAGTAGACCAGATTCTTCCCATTCTCGAGGCAAATAATGTTAGAGCCTACATAAACGGACATGACCACTGCTTGGAACACATGAGTAGCACCAATGGCCAAATACAATTCTTGACTACTGGTGGTGGCTCCAAGGCATGGAAAGGTGACCTCTCTAGGTTGAATCAAGGCGGGTATGAGTTTTACTACGATGGGCAAGGCTTCCTCTCTGCTGAAATTCGACCAACCGATGCAGAATTTGCATTCTACGATGTTTCCGGTAGAGTTTTGCATCGTTTCAATATATCCAATTCCAAGGGGCTTCACAGTTCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko04142 ko04380 ko05323 map00740 map01100 map04142 map04380 map05323
Pfam Domains
Protein Families
Metallophos

Protein Analysis

330

Amino Acids

37.85

Weight (kDa)

7.8

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 40 - 249 9.6e-18 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 748, 813
AccI GTMKAC 1 cut(s) 681
AciI CCGC 2 cut(s) 79, 846
AclWI GGATC 1 cut(s) 585
AcoI YGGCCR 2 cut(s) 126, 772
AcsI RAATTY 3 cut(s) 598, 891, 911
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 4 cut(s) 11, 335, 342, 497
AfeI AGCGCT 1 cut(s) 554
AfiI CCNNNNNNNGG 3 cut(s) 574, 748, 813
AgsI TTSAA 4 cut(s) 228, 574, 838, 955
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 4 cut(s) 68, 73, 120, 382
AluI AGCT 4 cut(s) 68, 73, 120, 382
Alw21I GWGCWC 1 cut(s) 90
AlwI GGATC 1 cut(s) 585
Ama87I CYCGRG 2 cut(s) 284, 701
Aor51HI AGCGCT 1 cut(s) 554
AoxI GGCC 2 cut(s) 126, 772
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 3 cut(s) 598, 891, 911
AspA2I CCTAGG 1 cut(s) 545
AspLEI GCGC 2 cut(s) 155, 555
AspS9I GGNCC 1 cut(s) 501
AsuHPI GGTGA 1 cut(s) 833
AvaI CYCGRG 2 cut(s) 284, 701
AvaII GGWCC 1 cut(s) 501
AvrII CCTAGG 1 cut(s) 545
AxyI CCTNAGG 1 cut(s) 425
BalI TGGCCA 1 cut(s) 774
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 562
BccI CCATC 3 cut(s) 178, 397, 860
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 671
BciVI GTATCC 2 cut(s) 291, 658
BfaI CTAG 2 cut(s) 546, 831
BfmI CTRYAG 1 cut(s) 358
BfoI RGCGCY 2 cut(s) 156, 556
BfuI GTATCC 2 cut(s) 291, 658
BisI GCNGC 1 cut(s) 551
BlnI CCTAGG 1 cut(s) 545
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 552
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 1 cut(s) 671
Bme18I GGWCC 1 cut(s) 501
BmeT110I CYCGRG 2 cut(s) 284, 701
BmgT120I GGNCC 1 cut(s) 501
BmiI GGNNCC 2 cut(s) 350, 805
BmrFI CCNGG 1 cut(s) 671
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 3 cut(s) 664, 895, 955
BmuI ACTGGG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 116
Bpu1102I GCTNAGC 1 cut(s) 69
BsaJI CCNNGG 4 cut(s) 545, 669, 807, 969
BsaWI WCCGGW 1 cut(s) 932
Bsc4I CCNNNNNNNGG 3 cut(s) 574, 748, 813
Bse118I RCCGGY 1 cut(s) 411
Bse1I ACTGG 5 cut(s) 235, 389, 503, 617, 799
Bse21I CCTNAGG 1 cut(s) 425
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 4 cut(s) 545, 669, 807, 969
BseLI CCNNNNNNNGG 3 cut(s) 574, 748, 813
BseMII CTCAG 5 cut(s) 60, 107, 326, 374, 416
BseNI ACTGG 5 cut(s) 235, 389, 503, 617, 799
BseXI GCAGC 1 cut(s) 562
BshFI GGCC 2 cut(s) 128, 774
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 2 cut(s) 284, 701
BsiSI CCGG 2 cut(s) 412, 933
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 3 cut(s) 574, 748, 813
BsmFI GGGAC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 2 cut(s) 128, 774
BsoBI CYCGRG 2 cut(s) 284, 701
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 1 cut(s) 577
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 2 cut(s) 79, 846
BspANI GGCC 2 cut(s) 128, 774
BspCNI CTCAG 5 cut(s) 61, 108, 325, 375, 417
BspLI GGNNCC 2 cut(s) 350, 805
BspPI GGATC 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 411
BsrI ACTGG 5 cut(s) 235, 389, 503, 617, 799
BssAI RCCGGY 1 cut(s) 411
BssECI CCNNGG 4 cut(s) 545, 669, 807, 969
BssMI GATC 1 cut(s) 577
BssT1I CCWWGG 3 cut(s) 545, 807, 969
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 1 cut(s) 983
Bst6I CTCTTC 1 cut(s) 142
BstAPI GCANNNNNTGC 1 cut(s) 914
BstDEI CTNAG 5 cut(s) 69, 116, 312, 383, 425
BstEII GGTNACC 1 cut(s) 821
BstENI CCTNNNNNAGG 1 cut(s) 572
BstH2I RGCGCY 2 cut(s) 156, 556
BstHHI GCGC 2 cut(s) 155, 555
BstKTI GATC 1 cut(s) 580
BstMBI GATC 1 cut(s) 577
BstMWI GCNNNNNNNGC 4 cut(s) 85, 520, 771, 914
BstNI CCWGG 1 cut(s) 671
BstNSI RCATGY 1 cut(s) 58
BstPI GGTNACC 1 cut(s) 821
BstSCI CCNGG 1 cut(s) 669
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 1 cut(s) 562
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 425
BsuI GTATCC 2 cut(s) 291, 658
BsuRI GGCC 2 cut(s) 128, 774
BtsI GCAGTG 2 cut(s) 306, 742
BtsIMutI CAGTG 3 cut(s) 66, 306, 742
CfoI GCGC 2 cut(s) 155, 555
Cfr10I RCCGGY 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 501
Csp6I GTAC 4 cut(s) 10, 334, 341, 496
CviAII CATG 6 cut(s) 55, 353, 737, 757, 813, 988
CviQI GTAC 4 cut(s) 10, 334, 341, 496
DdeI CTNAG 5 cut(s) 69, 116, 312, 383, 425
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
EaeI YGGCCR 2 cut(s) 126, 772
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Eco130I CCWWGG 3 cut(s) 545, 807, 969
Eco47I GGWCC 1 cut(s) 501
Eco47III AGCGCT 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 59
Eco81I CCTNAGG 1 cut(s) 425
Eco88I CYCGRG 2 cut(s) 284, 701
Eco91I GGTNACC 1 cut(s) 821
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoO65I GGTNACC 1 cut(s) 821
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 3 cut(s) 545, 807, 969
ErhI CCWWGG 3 cut(s) 545, 807, 969
FaeI CATG 6 cut(s) 58, 356, 740, 760, 816, 991
FalI AAGNNNNNCTT 2 cut(s) 139, 171
FaqI GGGAC 1 cut(s) 156
FatI CATG 6 cut(s) 54, 352, 736, 756, 812, 987
FauI CCCGC 1 cut(s) 839
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 1 cut(s) 551
Fsp4HI GCNGC 1 cut(s) 551
FspBI CTAG 2 cut(s) 546, 831
GlaI GCGC 2 cut(s) 154, 554
GluI GCNGC 1 cut(s) 551
HaeII RGCGCY 2 cut(s) 156, 556
HaeIII GGCC 2 cut(s) 128, 774
HapII CCGG 2 cut(s) 412, 933
HhaI GCGC 2 cut(s) 155, 555
Hin1II CATG 6 cut(s) 58, 356, 740, 760, 816, 991
Hin6I GCGC 2 cut(s) 153, 553
HinP1I GCGC 2 cut(s) 153, 553
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 5 cut(s) 110, 287, 385, 688, 838
HpaII CCGG 2 cut(s) 412, 933
HphI GGTGA 1 cut(s) 833
Hpy166II GTNNAC 5 cut(s) 10, 268, 442, 490, 682
Hpy188I TCNGA 6 cut(s) 134, 183, 197, 315, 399, 644
Hpy188III TCNNGA 5 cut(s) 284, 571, 581, 701, 787
Hpy8I GTNNAC 5 cut(s) 10, 268, 442, 490, 682
Hpy99I CGWCG 4 cut(s) 17, 20, 98, 134
HpyAV CCTTC 1 cut(s) 568
HpyCH4III ACNGT 1 cut(s) 983
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 8 cut(s) 54, 455, 590, 608, 629, 908, 917, 946
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 520, 771, 914
HpyF3I CTNAG 5 cut(s) 69, 116, 312, 383, 425
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 6 cut(s) 58, 356, 740, 760, 816, 991
HspAI GCGC 2 cut(s) 153, 553
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 1 cut(s) 809
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 3 cut(s) 664, 895, 955
MaeI CTAG 2 cut(s) 546, 831
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 821
MalI GATC 1 cut(s) 579
MboI GATC 1 cut(s) 577
MboII GAAGA 7 cut(s) 159, 386, 412, 454, 457, 628, 683
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 90
MlsI TGGCCA 1 cut(s) 774
MluCI AATT 7 cut(s) 203, 450, 598, 782, 891, 911, 964
MluNI TGGCCA 1 cut(s) 774
MlyI GAGTC 3 cut(s) 104, 296, 394
MmeI TCCRAC 2 cut(s) 112, 528
Mox20I TGGCCA 1 cut(s) 774
MscI TGGCCA 1 cut(s) 774
MseI TTAA 1 cut(s) 206
MslI CAYNNNNRTG 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 774
MspA1I CMGCKG 3 cut(s) 68, 79, 382
MspI CCGG 2 cut(s) 412, 933
MspR9I CCNGG 1 cut(s) 671
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 1 cut(s) 671
MwoI GCNNNNNNNGC 4 cut(s) 85, 520, 771, 914
NdeII GATC 1 cut(s) 577
NlaIII CATG 6 cut(s) 58, 356, 740, 760, 816, 991
NlaIV GGNNCC 2 cut(s) 350, 805
NmuCI GTSAC 1 cut(s) 821
NspI RCATGY 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 701
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 917
PfeI GAWTC 2 cut(s) 688, 838
PflMI CCANNNNNTGG 2 cut(s) 748, 813
PkrI GCNGC 1 cut(s) 552
PleI GAGTC 3 cut(s) 104, 295, 393
PpsI GAGTC 3 cut(s) 104, 295, 393
Psp6I CCWGG 1 cut(s) 669
PspEI GGTNACC 1 cut(s) 821
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 350, 805
PspPI GGNCC 1 cut(s) 501
PsrI GAACNNNNNNTAC 2 cut(s) 341, 373
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 2 cut(s) 68, 382
RsaI GTAC 4 cut(s) 11, 335, 342, 497
RsaNI GTAC 4 cut(s) 10, 334, 341, 496
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 1 cut(s) 551
Sau3AI GATC 1 cut(s) 577
Sau96I GGNCC 1 cut(s) 501
ScaI AGTACT 1 cut(s) 497
SchI GAGTC 3 cut(s) 104, 296, 394
ScrFI CCNGG 1 cut(s) 671
SduI GDGCHC 1 cut(s) 90
SfaNI GCATC 3 cut(s) 664, 895, 955
SfcI CTRYAG 1 cut(s) 358
Sfr274I CTCGAG 1 cut(s) 701
SinI GGWCC 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
Sse9I AATT 7 cut(s) 203, 450, 598, 782, 891, 911, 964
SsiI CCGC 2 cut(s) 79, 846
SspMI CTAG 2 cut(s) 546, 831
StyD4I CCNGG 1 cut(s) 669
StyI CCWWGG 3 cut(s) 545, 807, 969
TaaI ACNGT 1 cut(s) 983
TaiI ACGT 1 cut(s) 15
TaqI TCGA 4 cut(s) 471, 602, 702, 895
TasI AATT 7 cut(s) 203, 450, 598, 782, 891, 911, 964
TatI WGTACW 2 cut(s) 340, 495
TfiI GAWTC 2 cut(s) 688, 838
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TscAI CASTG 3 cut(s) 66, 313, 749
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 1 cut(s) 457
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 3 cut(s) 66, 313, 749
Van91I CCANNNNNTGG 2 cut(s) 748, 813
VpaK11BI GGWCC 1 cut(s) 501
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 3 cut(s) 598, 891, 911
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 701
XmaJI CCTAGG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 681
XspI CTAG 2 cut(s) 546, 831
ZrmI AGTACT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.