AT1G16445

Putative rRNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008049
Physical Location & Seq
Forward (+)
5617786 .. 5619456
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16445.1

Sequence Viewer

Length: 825 bp
ATGGCGGCTGGGTTCTTTCAAGCAGAGATGTCAATTTTATCCTCTACGTTGGCGCGCTCATACTCGTTACCTATCCGCAAAACGCTTATGACCTTCGATTTTCGAATTGCAATGCAACGAAACCCTTGTCTCAGAATTCGACGTTCTTGTGTAGCTGCTTTTAGCTCGACTCCTTCGCATTCACGAAATTTTCCAATTCGAGGACTAGAGGATGTTTTTGTTGGTTATCTATTTGGGAGGAAGAAGGCAACGGAAGTAGCTCATGTTGTGTGGGAGCAGGTAATCCAAAAGGGGGATACGGTCATTGATGCTACCTGTGGAAACGGGAACGATACTTTAGCAATGTTGAAGATGGTTATGCATGATTCTGTTGGTTGTGGTGGTTATGTCTATGCTATGGACATTCAAAAAGATGCCATTGAAAGCACTTCATCTTTGCTCGATCAAGCCGTTGGTTCAAAAGAGAAGGAATGCGTCAAACTTTTTAACCTCTGTCATAGTAAAATGGGAGAGATTGTCCCTGAAAACGCCCGAGTGAGGATGGTTGCATTCAATCTGGGGTATCTTCCAGGAGGCAACAAGTCGATAATCACTCTCTCGGATACAACATTATCGGCATTGAAAGCTGCTGAGAGAATCTTAAAGCCCGGTGGTCTCATCAGTTTGGTGGTTTACATTGGCCATCCTGGTGGAAGAGAAGAGTTAGAAGTGGTTGAAGCTTTTGGGTCGGGTTTACCGGTCAGTGATTGGATCTGCTGCAAATTCCAAATGTTGAACCGACCGTTAGCTCCAGTTCTTGTTTTCATGTTCAAGAGAGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
rRNA_methylase

Protein Analysis

274

Amino Acids

30.15

Weight (kDa)

8.75

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rRNA_methylase PF06962 130 - 270 3.5e-43 Putative rRNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccII CGCG 1 cut(s) 55
AciI CCGC 2 cut(s) 5, 76
AclWI GGATC 1 cut(s) 758
AcoI YGGCCR 1 cut(s) 679
AcsI RAATTY 3 cut(s) 135, 187, 761
AfiI CCNNNNNNNGG 3 cut(s) 200, 292, 537
AgeI ACCGGT 1 cut(s) 736
AjnI CCWGG 2 cut(s) 568, 685
AluBI AGCT 6 cut(s) 155, 165, 260, 626, 719, 788
AluI AGCT 6 cut(s) 155, 165, 260, 626, 719, 788
Alw26I GTCTC 2 cut(s) 134, 659
AlwI GGATC 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 531
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 3 cut(s) 155, 626, 756
ApoI RAATTY 3 cut(s) 135, 187, 761
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
AsiGI ACCGGT 1 cut(s) 736
AspLEI GCGC 2 cut(s) 55, 57
AsuC2I CCSGG 1 cut(s) 648
AsuII TTCGAA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 531
BalI TGGCCA 1 cut(s) 681
BbvI GCAGC 3 cut(s) 142, 613, 743
BccI CCATC 3 cut(s) 346, 535, 690
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 2 cut(s) 570, 687
BciVI GTATCC 2 cut(s) 289, 595
BcnI CCSGG 1 cut(s) 648
BcoDI GTCTC 2 cut(s) 134, 659
BfaI CTAG 1 cut(s) 206
BfuAI ACCTGC 1 cut(s) 268
BfuI GTATCC 2 cut(s) 289, 595
BisI GCNGC 4 cut(s) 6, 156, 627, 757
BlsI GCNGC 4 cut(s) 7, 157, 628, 758
Bme1390I CCNGG 3 cut(s) 570, 648, 687
BmeT110I CYCGRG 1 cut(s) 531
BmrFI CCNGG 3 cut(s) 570, 648, 687
BmsI GCATC 2 cut(s) 298, 403
BpmI CTGGAG 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 659
BsaWI WCCGGW 1 cut(s) 736
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 292, 537
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 1 cut(s) 791
Bse3DI GCAATG 2 cut(s) 117, 348
BseBI CCWGG 2 cut(s) 570, 687
BseGI GGATG 3 cut(s) 217, 546, 682
BseLI CCNNNNNNNGG 3 cut(s) 200, 292, 537
BseMI GCAATG 2 cut(s) 117, 348
BseMII CTCAG 2 cut(s) 145, 621
BseNI ACTGG 1 cut(s) 791
BsePI GCGCGC 1 cut(s) 53
BseXI GCAGC 3 cut(s) 142, 613, 743
BseYI CCCAGC 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 782
BshFI GGCC 1 cut(s) 681
BshTI ACCGGT 1 cut(s) 736
BsiEI CGRYCG 1 cut(s) 782
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 2 cut(s) 648, 737
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 3 cut(s) 200, 292, 537
BsmAI GTCTC 2 cut(s) 134, 659
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 3 cut(s) 178, 476, 548
BsnI GGCC 1 cut(s) 681
Bso31I GGTCTC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 531
Bsp119I TTCGAA 1 cut(s) 103
Bsp143I GATC 2 cut(s) 442, 750
BspACI CCGC 2 cut(s) 5, 76
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 2 cut(s) 144, 622
BspFNI CGCG 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 1 cut(s) 758
BspT104I TTCGAA 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 659
BsrDI GCAATG 2 cut(s) 117, 348
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 1 cut(s) 791
BssAI RCCGGY 1 cut(s) 736
BssHII GCGCGC 1 cut(s) 53
BssMI GATC 2 cut(s) 442, 750
Bst2UI CCWGG 2 cut(s) 570, 687
Bst4CI ACNGT 2 cut(s) 301, 783
Bst6I CTCTTC 2 cut(s) 688, 693
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 131, 630
BstF5I GGATG 3 cut(s) 217, 546, 682
BstFNI CGCG 1 cut(s) 55
BstHHI GCGC 2 cut(s) 55, 57
BstKTI GATC 2 cut(s) 445, 753
BstMAI GTCTC 2 cut(s) 134, 659
BstMBI GATC 2 cut(s) 442, 750
BstMCI CGRYCG 1 cut(s) 782
BstMWI GCNNNNNNNGC 1 cut(s) 623
BstNI CCWGG 2 cut(s) 570, 687
BstSCI CCNGG 3 cut(s) 568, 646, 685
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 3 cut(s) 142, 613, 743
BstX2I RGATCY 1 cut(s) 750
BstXI CCANNNNNNTGG 1 cut(s) 689
BstYI RGATCY 1 cut(s) 750
BsuI GTATCC 2 cut(s) 289, 595
BsuRI GGCC 1 cut(s) 681
BtsCI GGATG 3 cut(s) 217, 546, 682
BtsIMutI CAGTG 1 cut(s) 748
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 2 cut(s) 55, 57
Cfr10I RCCGGY 1 cut(s) 736
CseI GACGC 1 cut(s) 463
CspAI ACCGGT 1 cut(s) 736
CviAII CATG 3 cut(s) 263, 362, 805
DdeI CTNAG 2 cut(s) 131, 630
DpnI GATC 2 cut(s) 444, 752
DpnII GATC 2 cut(s) 442, 750
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 688, 693
EarI CTCTTC 2 cut(s) 688, 693
Eco31I GGTCTC 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 2 cut(s) 568, 685
EcoT22I ATGCAT 1 cut(s) 363
FaeI CATG 3 cut(s) 266, 365, 808
FaqI GGGAC 1 cut(s) 503
FatI CATG 3 cut(s) 262, 361, 804
Fnu4HI GCNGC 4 cut(s) 6, 156, 627, 757
FokI GGATG 3 cut(s) 224, 553, 669
Fsp4HI GCNGC 4 cut(s) 6, 156, 627, 757
FspBI CTAG 1 cut(s) 206
GlaI GCGC 2 cut(s) 54, 56
GluI GCNGC 4 cut(s) 6, 156, 627, 757
GsaI CCCAGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 774
HaeIII GGCC 1 cut(s) 681
HapII CCGG 2 cut(s) 648, 737
HgaI GACGC 1 cut(s) 463
HhaI GCGC 2 cut(s) 55, 57
Hin1II CATG 3 cut(s) 266, 365, 808
Hin6I GCGC 2 cut(s) 53, 55
HinP1I GCGC 2 cut(s) 53, 55
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 3 cut(s) 169, 365, 636
HpaII CCGG 2 cut(s) 648, 737
Hpy166II GTNNAC 2 cut(s) 673, 734
Hpy188I TCNGA 2 cut(s) 134, 601
Hpy188III TCNNGA 2 cut(s) 183, 811
Hpy8I GTNNAC 2 cut(s) 673, 734
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 4 cut(s) 103, 183, 238, 460
HpyCH4III ACNGT 2 cut(s) 301, 783
HpyCH4IV ACGT 2 cut(s) 47, 142
HpyCH4V TGCA 5 cut(s) 110, 115, 361, 548, 759
HpyF10VI GCNNNNNNNGC 1 cut(s) 623
HpyF3I CTNAG 2 cut(s) 131, 630
HpySE526I ACGT 2 cut(s) 47, 142
Hsp92II CATG 3 cut(s) 266, 365, 808
HspAI GCGC 2 cut(s) 53, 55
Kzo9I GATC 2 cut(s) 442, 750
LmnI GCTCC 2 cut(s) 274, 793
Lsp1109I GCAGC 3 cut(s) 142, 613, 743
LweI GCATC 2 cut(s) 298, 403
MaeI CTAG 1 cut(s) 206
MaeII ACGT 2 cut(s) 47, 142
MaeIII GTNAC 1 cut(s) 66
MalI GATC 2 cut(s) 444, 752
MboI GATC 2 cut(s) 442, 750
MboII GAAGA 5 cut(s) 253, 361, 557, 705, 710
MflI RGATCY 1 cut(s) 750
MlsI TGGCCA 1 cut(s) 681
MluCI AATT 6 cut(s) 33, 105, 135, 187, 195, 761
MluNI TGGCCA 1 cut(s) 681
MlyI GAGTC 1 cut(s) 163
MnlI CCTC 7 cut(s) 52, 194, 202, 231, 500, 531, 566
Mox20I TGGCCA 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 363
MscI TGGCCA 1 cut(s) 681
MseI TTAA 2 cut(s) 486, 641
MslI CAYNNNNRTG 1 cut(s) 687
Msp20I TGGCCA 1 cut(s) 681
MspI CCGG 2 cut(s) 648, 737
MspR9I CCNGG 3 cut(s) 570, 648, 687
Mva1269I GAATGC 3 cut(s) 178, 476, 548
MvaI CCWGG 2 cut(s) 570, 687
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 623
NciI CCSGG 1 cut(s) 648
NdeII GATC 2 cut(s) 442, 750
NlaIII CATG 3 cut(s) 266, 365, 808
NsiI ATGCAT 1 cut(s) 363
NspV TTCGAA 1 cut(s) 103
PauI GCGCGC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 3 cut(s) 178, 476, 548
PfeI GAWTC 2 cut(s) 365, 636
PfoI TCCNGGA 1 cut(s) 568
PinAI ACCGGT 1 cut(s) 736
PkrI GCNGC 4 cut(s) 7, 157, 628, 758
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 2 cut(s) 568, 685
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 2 cut(s) 568, 685
PsuI RGATCY 1 cut(s) 750
PteI GCGCGC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 687
SaqAI TTAA 2 cut(s) 486, 641
SatI GCNGC 4 cut(s) 6, 156, 627, 757
Sau3AI GATC 2 cut(s) 442, 750
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 3 cut(s) 570, 648, 687
SfaNI GCATC 2 cut(s) 298, 403
SfuI TTCGAA 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 687
Sse9I AATT 6 cut(s) 33, 105, 135, 187, 195, 761
SsiI CCGC 2 cut(s) 5, 76
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 3 cut(s) 568, 646, 685
TaaI ACNGT 2 cut(s) 301, 783
TaiI ACGT 2 cut(s) 50, 145
TaqI TCGA 7 cut(s) 96, 103, 139, 167, 199, 441, 584
TasI AATT 6 cut(s) 33, 105, 135, 187, 195, 761
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 365, 636
Tru1I TTAA 2 cut(s) 486, 641
Tru9I TTAA 2 cut(s) 486, 641
TscAI CASTG 1 cut(s) 748
TseI GCWGC 3 cut(s) 155, 626, 756
TspDTI ATGAA 2 cut(s) 420, 793
TspGWI ACGGA 1 cut(s) 266
TspRI CASTG 1 cut(s) 748
XapI RAATTY 3 cut(s) 135, 187, 761
XspI CTAG 1 cut(s) 206
Zsp2I ATGCAT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.