Prupe.8G055300_v2.0.a1

Putative rRNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0008049
Physical Location & Seq
Forward (+)
6456752 .. 6461528
4777 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G055300.1

Sequence Viewer

Length: 852 bp
ATGTTGGCACTGCCGCTGAGATTAGGCGCGCATTGGCCTGCGGTACAAAAACCTCTAATATCAACTTCAACACGGTGGTTCGTTTTCCCCTATCCGCGAAACGTTTCGCTGAATGGCAATAGCCCCAGAGACCACTTTGACCGAAACGTTTCGAGGATCCCTGCGAATTCAACATCTATCACTGGCAAGACCAATGATTTCGTTTCCGTTTCTCTCCCCAAAGACTCTCCTCTGTTTGGCTTGGAGGATTTACTGGTGAGCTTTATCTTGGGCAAGAAGAGGGCCACAGAAGTTTCTCACTTGGTTTGGAAAAGTGTTGTCCAAAAAGGAGATACAGTCATTGATGCCACTTGTGGCAATGGTCATGATACCTTAGCAATGCTGAAGATGGTTGCTGATGAATCGTGTAAGGGTTCTGTTTACGGGCTGGATGTTCAGGAAGCTGCTTTACAGAAAACTTCTTCTTTGCTGGAAGAGTCGGTCGGTCCAAGTGAGAAAGAACTTATTAAGCTCTTCTCCAAATGCCACAGTAAAATGGATGAAGTTCTTCCAAAAGATACATCTGTCAGGCTTGTCGCTTTCAATCTTGGCTATCTTCCTGGGGGTGACAAAACAATTATCACACAGTCAGAAACAACACTGAAGGCATTGGAAGTTGCAAAAAGTATCATGGTGCCTGGAGGTCTTATCAGTATAGTGGTTTATGTGGGGCATCCTGGTGGATGGGAAGAACTGAAGACTATCCGAGACTTCATCTCCAAATTATCAGTTGAGAAATGGATCTGCTATGAGTTTCAGACAGTAGACCGATCATGGTCTCCTATACTTATTTTCTTGTTCAAGAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
rRNA_methylase

Protein Analysis

284

Amino Acids

31.28

Weight (kDa)

8.5

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 673
AccI GTMKAC 1 cut(s) 804
AccII CGCG 2 cut(s) 29, 97
AciI CCGC 3 cut(s) 14, 41, 95
AclI AACGTT 2 cut(s) 102, 147
AclWI GGATC 3 cut(s) 151, 164, 788
AcsI RAATTY 1 cut(s) 166
AcuI CTGAAG 3 cut(s) 404, 662, 755
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 4 cut(s) 69, 171, 583, 841
AjnI CCWGG 3 cut(s) 598, 676, 715
AluBI AGCT 3 cut(s) 261, 443, 511
AluI AGCT 3 cut(s) 261, 443, 511
Alw26I GTCTC 3 cut(s) 123, 741, 822
AlwI GGATC 3 cut(s) 151, 164, 788
AoxI GGCC 2 cut(s) 35, 282
ApeKI GCWGC 1 cut(s) 443
ApoI RAATTY 1 cut(s) 166
Asp700I GAANNNNTTC 3 cut(s) 103, 148, 546
AspLEI GCGC 2 cut(s) 29, 31
AspS9I GGNCC 2 cut(s) 282, 485
AsuHPI GGTGA 2 cut(s) 268, 617
AvaII GGWCC 1 cut(s) 485
BaeI ACNNNNGTAYC 2 cut(s) 549, 582
BamHI GGATCC 1 cut(s) 156
BanI GGYRCC 1 cut(s) 673
BarI GAAGNNNNNNTAC 2 cut(s) 432, 464
BbsI GAAGAC 1 cut(s) 743
BbvI GCAGC 1 cut(s) 430
BccI CCATC 2 cut(s) 382, 717
BciT130I CCWGG 3 cut(s) 600, 678, 717
BcoDI GTCTC 3 cut(s) 123, 741, 822
BisI GCNGC 2 cut(s) 14, 444
BlsI GCNGC 2 cut(s) 15, 445
Bme1390I CCNGG 3 cut(s) 600, 678, 717
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 282, 485
BmiI GGNNCC 2 cut(s) 158, 675
BmrFI CCNGG 3 cut(s) 600, 678, 717
BmsI GCATC 2 cut(s) 334, 721
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 1 cut(s) 373
BsaI GGTCTC 2 cut(s) 123, 822
BsaJI CCNNGG 1 cut(s) 599
BsaXI ACNNNNNCTCC 4 cut(s) 321, 351, 740, 770
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 187, 258
Bse3DI GCAATG 2 cut(s) 364, 384
BseBI CCWGG 3 cut(s) 600, 678, 717
BseDI CCNNGG 1 cut(s) 599
BseGI GGATG 4 cut(s) 436, 544, 712, 728
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 2 cut(s) 364, 384
BseMII CTCAG 1 cut(s) 8
BseNI ACTGG 2 cut(s) 187, 258
BsePI GCGCGC 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 219
BseXI GCAGC 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 29, 97
Bsh1285I CGRYCG 1 cut(s) 483
BshFI GGCC 2 cut(s) 37, 284
BshNI GGYRCC 1 cut(s) 673
BsiEI CGRYCG 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 3 cut(s) 123, 741, 822
BsnI GGCC 2 cut(s) 37, 284
Bso31I GGTCTC 2 cut(s) 123, 822
Bsp143I GATC 3 cut(s) 156, 780, 809
BspACI CCGC 3 cut(s) 14, 41, 95
BspANI GGCC 2 cut(s) 37, 284
BspCNI CTCAG 1 cut(s) 9
BspFNI CGCG 2 cut(s) 29, 97
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 158, 675
BspPI GGATC 3 cut(s) 151, 164, 788
BspQI GCTCTTC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 673
BspTNI GGTCTC 2 cut(s) 123, 822
BsrDI GCAATG 2 cut(s) 364, 384
BsrI ACTGG 2 cut(s) 187, 258
BssECI CCNNGG 1 cut(s) 599
BssHII GCGCGC 1 cut(s) 27
BssMI GATC 3 cut(s) 156, 780, 809
Bst2UI CCWGG 3 cut(s) 600, 678, 717
Bst4CI ACNGT 5 cut(s) 75, 337, 530, 627, 802
Bst6I CTCTTC 3 cut(s) 272, 468, 518
BstC8I GCNNGC 2 cut(s) 29, 39
BstDEI CTNAG 2 cut(s) 17, 373
BstF5I GGATG 4 cut(s) 436, 544, 712, 728
BstFNI CGCG 2 cut(s) 29, 97
BstHHI GCGC 2 cut(s) 29, 31
BstKTI GATC 3 cut(s) 159, 783, 812
BstMAI GTCTC 3 cut(s) 123, 741, 822
BstMBI GATC 3 cut(s) 156, 780, 809
BstMCI CGRYCG 1 cut(s) 483
BstNI CCWGG 3 cut(s) 600, 678, 717
BstSCI CCNGG 3 cut(s) 598, 676, 715
BstUI CGCG 2 cut(s) 29, 97
BstV1I GCAGC 1 cut(s) 430
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 156, 780
BstYI RGATCY 2 cut(s) 156, 780
BsuRI GGCC 2 cut(s) 37, 284
BtsCI GGATG 4 cut(s) 436, 544, 712, 728
BtsI GCAGTG 1 cut(s) 8
BtsIMutI CAGTG 3 cut(s) 8, 180, 638
Cac8I GCNNGC 2 cut(s) 29, 39
CciI TCATGA 1 cut(s) 364
CfoI GCGC 2 cut(s) 29, 31
Cfr13I GGNCC 2 cut(s) 282, 485
Csp6I GTAC 1 cut(s) 44
CviAII CATG 3 cut(s) 365, 670, 813
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 2 cut(s) 17, 373
DpnI GATC 3 cut(s) 158, 782, 811
DpnII GATC 3 cut(s) 156, 780, 809
Eam1104I CTCTTC 3 cut(s) 272, 468, 518
EarI CTCTTC 3 cut(s) 272, 468, 518
Eco31I GGTCTC 2 cut(s) 123, 822
Eco47I GGWCC 1 cut(s) 485
Eco57I CTGAAG 3 cut(s) 404, 662, 755
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 598, 676, 715
FaeI CATG 3 cut(s) 368, 673, 816
FaiI YATR 7 cut(s) 366, 671, 695, 705, 789, 814, 824
FatI CATG 3 cut(s) 364, 669, 812
FblI GTMKAC 1 cut(s) 804
Fnu4HI GCNGC 2 cut(s) 14, 444
FokI GGATG 4 cut(s) 443, 551, 699, 735
Fsp4HI GCNGC 2 cut(s) 14, 444
GlaI GCGC 2 cut(s) 28, 30
GluI GCNGC 2 cut(s) 14, 444
GsuI CTGGAG 1 cut(s) 699
HaeIII GGCC 2 cut(s) 37, 284
HhaI GCGC 2 cut(s) 29, 31
Hin1II CATG 3 cut(s) 368, 673, 816
Hin6I GCGC 2 cut(s) 27, 29
HinP1I GCGC 2 cut(s) 27, 29
HinfI GANTC 3 cut(s) 224, 401, 476
HphI GGTGA 2 cut(s) 268, 617
Hpy166II GTNNAC 2 cut(s) 421, 805
Hpy188I TCNGA 3 cut(s) 631, 746, 798
Hpy188III TCNNGA 3 cut(s) 365, 437, 841
Hpy8I GTNNAC 2 cut(s) 421, 805
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 5 cut(s) 75, 337, 530, 627, 802
HpyCH4IV ACGT 2 cut(s) 102, 147
HpyCH4V TGCA 1 cut(s) 659
HpyF3I CTNAG 2 cut(s) 17, 373
HpySE526I ACGT 2 cut(s) 102, 147
Hsp92II CATG 3 cut(s) 368, 673, 816
HspAI GCGC 2 cut(s) 27, 29
Kzo9I GATC 3 cut(s) 156, 780, 809
LguI GCTCTTC 1 cut(s) 518
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 2 cut(s) 334, 721
MaeII ACGT 2 cut(s) 102, 147
MaeIII GTNAC 1 cut(s) 605
MalI GATC 3 cut(s) 158, 782, 811
MboI GATC 3 cut(s) 156, 780, 809
MboII GAAGA 9 cut(s) 289, 397, 453, 485, 505, 539, 587, 740, 748
MflI RGATCY 2 cut(s) 156, 780
MluCI AATT 3 cut(s) 166, 615, 761
MlyI GAGTC 2 cut(s) 218, 485
MnlI CCTC 6 cut(s) 63, 147, 238, 240, 273, 674
MroXI GAANNNNTTC 3 cut(s) 103, 148, 546
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 717
MspA1I CMGCKG 1 cut(s) 16
MspR9I CCNGG 3 cut(s) 600, 678, 717
MvaI CCWGG 3 cut(s) 600, 678, 717
MvnI CGCG 2 cut(s) 29, 97
NdeII GATC 3 cut(s) 156, 780, 809
NlaIII CATG 3 cut(s) 368, 673, 816
NlaIV GGNNCC 2 cut(s) 158, 675
NmuCI GTSAC 1 cut(s) 605
PagI TCATGA 1 cut(s) 364
PauI GCGCGC 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 518
PdmI GAANNNNTTC 3 cut(s) 103, 148, 546
PfeI GAWTC 1 cut(s) 401
PkrI GCNGC 2 cut(s) 15, 445
PleI GAGTC 2 cut(s) 218, 484
PpsI GAGTC 2 cut(s) 218, 484
Psp1406I AACGTT 2 cut(s) 102, 147
Psp6I CCWGG 3 cut(s) 598, 676, 715
PspGI CCWGG 3 cut(s) 598, 676, 715
PspN4I GGNNCC 2 cut(s) 158, 675
PspPI GGNCC 2 cut(s) 282, 485
PsuI RGATCY 2 cut(s) 156, 780
PteI GCGCGC 1 cut(s) 27
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
RseI CAYNNNNRTG 1 cut(s) 717
SapI GCTCTTC 1 cut(s) 518
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 2 cut(s) 14, 444
Sau3AI GATC 3 cut(s) 156, 780, 809
Sau96I GGNCC 2 cut(s) 282, 485
SchI GAGTC 2 cut(s) 218, 485
ScrFI CCNGG 3 cut(s) 600, 678, 717
SetI ASST 8 cut(s) 55, 105, 150, 263, 374, 445, 513, 685
SfaNI GCATC 2 cut(s) 334, 721
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 717
Sse9I AATT 3 cut(s) 166, 615, 761
SsiI CCGC 3 cut(s) 14, 41, 95
StyD4I CCNGG 3 cut(s) 598, 676, 715
TaaI ACNGT 5 cut(s) 75, 337, 530, 627, 802
TaiI ACGT 2 cut(s) 105, 150
TaqI TCGA 1 cut(s) 152
TaqII GACCGA 4 cut(s) 156, 469, 473, 822
TasI AATT 3 cut(s) 166, 615, 761
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 1 cut(s) 401
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 3 cut(s) 15, 187, 645
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 3 cut(s) 414, 555, 742
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 3 cut(s) 15, 187, 645
VpaK11BI GGWCC 1 cut(s) 485
XapI RAATTY 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 804
XmnI GAANNNNTTC 3 cut(s) 103, 148, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.