Rh6BG183800

Putative rRNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008049
Physical Location & Seq
Forward (+)
33812110 .. 33831429
19320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG183800.1

Sequence Viewer

Length: 816 bp
ATGTTGGCAGTTGTGCCACGGAGGTTATCATGGGGGCTCCCTCATTTGCTGAGCACACAGAAGCAGAAACCTCCAACGCGTACGTTCCGAAACTTTTCGGGTAATTCTGCCGGAAACCGCTGGTTGGGCAAGTTTGAACTGAACGCTGCAACTTCCAACGACTTGGTTTCGGTTTCTGTGCCCAAACACTCTCCTCTCTCTGGATTGGAGGATCTACTTTTGAGCTTTATCTCGGGCAAGAAGAAGGCCACAGAAGTTGCTCACTCGGTTTGGAAACGTGTGGTCCAAAAAGGAGATACGGTAATTGATGCCACTTGTGGCAATGGCCATGATACCTTAGCAATGCTCAAGATGGTAGCTGATGAATCGGGTAAGGGTTCAGTTTATGGAATGGATATTCAGGAAGTTGCTTTACAGAAAACTTCTTCTTTGCTGGAAGAGTCGGTCAGTCCAAAAGAGAAAGAACTTGTTAAGCTCTTCTCCAAATGTCACAGTAAAATGCGTGAAGTTCTTCCGCAGGATACATGTGTTAGGCTAGTTGCTTTCAACCTAGGCTATCTTCCTGGTGGCGACAAAACAATAATCACTGAATCAGGAACAACACTGAAAGCACTGGAAGTTGCAAAAGATATCCTACTGCCAGGAGGGCTTATCAGCTTAGTGGTTTATGTGGGGCATCCTGGTGGATGGGAAGAACTGCAGATGGTACAACACTTTGTTTCTAAACTATCCGTTGAGAAATGGATCTGCTGTGAGTATCAGTCATCAAACCGATCATGGTCGCCAATACTTGTTTTCTTATTCAAAAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
rRNA_methylase

Protein Analysis

271

Amino Acids

29.86

Weight (kDa)

9.06

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 97 - 143 1.8e-06 Methyltransferase domain
rRNA_methylase PF06962 128 - 268 4.3e-42 Putative rRNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 79
AciI CCGC 2 cut(s) 118, 515
AclWI GGATC 2 cut(s) 219, 752
AcoI YGGCCR 1 cut(s) 325
AfaI GTAC 2 cut(s) 82, 708
AfiI CCNNNNNNNGG 2 cut(s) 124, 200
AflIII ACRYGT 3 cut(s) 77, 277, 524
AgsI TTSAA 3 cut(s) 137, 547, 805
AjnI CCWGG 3 cut(s) 562, 640, 679
AluBI AGCT 4 cut(s) 225, 359, 475, 657
AluI AGCT 4 cut(s) 225, 359, 475, 657
Alw21I GWGCWC 1 cut(s) 56
AlwI GGATC 2 cut(s) 219, 752
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 2 cut(s) 246, 325
ApeKI GCWGC 1 cut(s) 146
Asp700I GAANNNNTTC 2 cut(s) 94, 510
AspA2I CCTAGG 1 cut(s) 550
AspS9I GGNCC 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 232
AvaII GGWCC 1 cut(s) 283
AvrII CCTAGG 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 183
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BalI TGGCCA 1 cut(s) 327
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 396, 428
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 133
BccI CCATC 3 cut(s) 346, 681, 697
BciT130I CCWGG 3 cut(s) 564, 642, 681
BciVI GTATCC 1 cut(s) 514
BfaI CTAG 2 cut(s) 536, 551
BfmI CTRYAG 1 cut(s) 698
BfuI GTATCC 1 cut(s) 514
BglI GCCNNNNNGGC 1 cut(s) 646
BisI GCNGC 1 cut(s) 147
BlnI CCTAGG 1 cut(s) 550
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 3 cut(s) 564, 642, 681
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 3 cut(s) 564, 642, 681
BmsI GCATC 3 cut(s) 298, 685, 800
Bpu10I CCTNAGC 1 cut(s) 337
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 17, 550
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 200
Bse1I ACTGG 1 cut(s) 618
Bse3DI GCAATG 2 cut(s) 328, 348
BseBI CCWGG 3 cut(s) 564, 642, 681
BseDI CCNNGG 2 cut(s) 17, 550
BseGI GGATG 2 cut(s) 676, 692
BseLI CCNNNNNNNGG 2 cut(s) 124, 200
BseMI GCAATG 2 cut(s) 328, 348
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 618
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 2 cut(s) 248, 327
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 1 cut(s) 111
BsiWI CGTACG 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 124, 200
BsnI GGCC 2 cut(s) 248, 327
BsoBI CYCGRG 1 cut(s) 232
Bsp1286I GDGCHC 3 cut(s) 39, 56, 183
Bsp143I GATC 3 cut(s) 211, 744, 773
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 2 cut(s) 118, 515
BspANI GGCC 2 cut(s) 248, 327
BspCNI CTCAG 1 cut(s) 42
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 702
BspPI GGATC 2 cut(s) 219, 752
BspQI GCTCTTC 1 cut(s) 482
BsrDI GCAATG 2 cut(s) 328, 348
BsrI ACTGG 1 cut(s) 618
BssECI CCNNGG 2 cut(s) 17, 550
BssMI GATC 3 cut(s) 211, 744, 773
BssT1I CCWWGG 1 cut(s) 550
Bst2UI CCWGG 3 cut(s) 564, 642, 681
Bst4CI ACNGT 2 cut(s) 301, 494
Bst6I CTCTTC 2 cut(s) 432, 482
BstDEI CTNAG 3 cut(s) 50, 337, 658
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 2 cut(s) 676, 692
BstFNI CGCG 1 cut(s) 79
BstKTI GATC 3 cut(s) 214, 747, 776
BstMBI GATC 3 cut(s) 211, 744, 773
BstMWI GCNNNNNNNGC 2 cut(s) 126, 646
BstNI CCWGG 3 cut(s) 564, 642, 681
BstNSI RCATGY 1 cut(s) 528
BstSCI CCNGG 3 cut(s) 562, 640, 679
BstSFI CTRYAG 1 cut(s) 698
BstSLI GKGCMC 1 cut(s) 183
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 2 cut(s) 211, 744
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 2 cut(s) 211, 744
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 2 cut(s) 248, 327
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 2 cut(s) 676, 692
BtsIMutI CAGTG 3 cut(s) 585, 602, 611
Cfr13I GGNCC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 81, 707
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 4 cut(s) 30, 329, 525, 777
CviQI GTAC 2 cut(s) 81, 707
DdeI CTNAG 3 cut(s) 50, 337, 658
DpnI GATC 3 cut(s) 213, 746, 775
DpnII GATC 3 cut(s) 211, 744, 773
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 432, 482
EarI CTCTTC 2 cut(s) 432, 482
Eco130I CCWWGG 1 cut(s) 550
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 232
EcoRII CCWGG 3 cut(s) 562, 640, 679
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 4 cut(s) 33, 332, 528, 780
FaiI YATR 6 cut(s) 31, 330, 387, 526, 669, 778
FatI CATG 4 cut(s) 29, 328, 524, 776
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 2 cut(s) 663, 699
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 2 cut(s) 536, 551
GluI GCNGC 1 cut(s) 147
HaeIII GGCC 2 cut(s) 248, 327
HapII CCGG 1 cut(s) 111
Hin1II CATG 4 cut(s) 33, 332, 528, 780
HinfI GANTC 3 cut(s) 365, 440, 590
HpaII CCGG 1 cut(s) 111
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 4 cut(s) 201, 349, 401, 594
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 2 cut(s) 301, 494
HpyCH4IV ACGT 2 cut(s) 83, 277
HpyCH4V TGCA 3 cut(s) 149, 623, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 646
HpyF3I CTNAG 3 cut(s) 50, 337, 658
HpySE526I ACGT 2 cut(s) 83, 277
Hsp92II CATG 4 cut(s) 33, 332, 528, 780
Kzo9I GATC 3 cut(s) 211, 744, 773
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 3 cut(s) 298, 685, 800
MaeI CTAG 2 cut(s) 536, 551
MaeII ACGT 2 cut(s) 83, 277
MaeIII GTNAC 1 cut(s) 488
MalI GATC 3 cut(s) 213, 746, 775
MboI GATC 3 cut(s) 211, 744, 773
MboII GAAGA 7 cut(s) 253, 417, 449, 469, 503, 551, 704
MflI RGATCY 2 cut(s) 211, 744
MhlI GDGCHC 3 cut(s) 39, 56, 183
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 2 cut(s) 103, 303
MluI ACGCGT 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 98, 180
MnlI CCTC 6 cut(s) 15, 51, 81, 202, 204, 638
Mox20I TGGCCA 1 cut(s) 327
MroXI GAANNNNTTC 2 cut(s) 94, 510
MscI TGGCCA 1 cut(s) 327
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 681
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 3 cut(s) 564, 642, 681
MvaI CCWGG 3 cut(s) 564, 642, 681
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 126, 646
NdeII GATC 3 cut(s) 211, 744, 773
NlaIII CATG 4 cut(s) 33, 332, 528, 780
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 488
NspI RCATGY 1 cut(s) 528
PciI ACATGT 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 482
PdmI GAANNNNTTC 2 cut(s) 94, 510
PfeI GAWTC 2 cut(s) 365, 590
Pfl23II CGTACG 1 cut(s) 80
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PscI ACATGT 1 cut(s) 524
Psp6I CCWGG 3 cut(s) 562, 640, 679
PspGI CCWGG 3 cut(s) 562, 640, 679
PspLI CGTACG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 1 cut(s) 283
PstI CTGCAG 1 cut(s) 702
PsuI RGATCY 2 cut(s) 211, 744
RsaI GTAC 2 cut(s) 82, 708
RsaNI GTAC 2 cut(s) 81, 707
RseI CAYNNNNRTG 1 cut(s) 681
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 3 cut(s) 211, 744, 773
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 564, 642, 681
SduI GDGCHC 3 cut(s) 39, 56, 183
SfaNI GCATC 3 cut(s) 298, 685, 800
SfcI CTRYAG 1 cut(s) 698
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 2 cut(s) 103, 303
SsiI CCGC 2 cut(s) 118, 515
SspMI CTAG 2 cut(s) 536, 551
StyD4I CCNGG 3 cut(s) 562, 640, 679
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 2 cut(s) 301, 494
TaiI ACGT 2 cut(s) 86, 280
TaqII GACCGA 1 cut(s) 433
TasI AATT 2 cut(s) 103, 303
TfiI GAWTC 2 cut(s) 365, 590
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 3 cut(s) 592, 609, 618
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 1 cut(s) 378
TspGWI ACGGA 2 cut(s) 34, 721
TspRI CASTG 3 cut(s) 592, 609, 618
VpaK11BI GGWCC 1 cut(s) 283
XceI RCATGY 1 cut(s) 528
XmaJI CCTAGG 1 cut(s) 550
XmnI GAANNNNTTC 2 cut(s) 94, 510
XspI CTAG 2 cut(s) 536, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.