AT1G18070

Elongation factor Tu C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004512
Physical Location & Seq
Reverse (-)
6213651 .. 6218384
4734 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18070.1

Sequence Viewer

Length: 1599 bp
ATGGATCTTGAGGCTGAGATCCGCGCATTACAGCTTGAATCGGCAGATGAAAACAATGGTGTTGTCATTCCAGAAGTTCATAACTCACATGAAGTTGAGAATTTGGATAAAGCACCGGAAGACTTGAAAGATGAAGTGCAAGAATCAATTCCAGTTCCTGATGAGCAAGAAGCTTCTGAGGATCATGATGAAGTAATGCTTCATCCAGTCCATAATCCAGCGAAAGCTAAAGAGAAAGCTGCTCAAGAGAAGGCTGCCAAAGAGGAAGCTGAAGATGTTGCAGAAGCAAATAAAAAGAGACACTTGAATGTTGTGTTCATTGGGCATGTTGATGCTGGTAAGTCCACAATTGGAGGACAGATTCTTTTTCTTAGTGGTCAGGTGGATGATCGACAAATCCAAAAGTATGAGAAGGAAGCAAAAGACAAAAGTAGAGAAAGCTGGTATATGGCATATATAATGGATACAAATGAAGAAGAGAGGCTTAAGGGCAAAACAGTTGAAGTTGGAAGGGCTCACTTTGAAACGGAGAGCACTAGATTCACTATTTTAGATGCTCCGGGGCACAAGAGTTACGTACCCAATATGATTAGTGGAGCATCTCAAGCGGACATTGGTGTGCTGGTGATTTCAGCTCGAAAAGGTGAATTTGAAACAGGATATGAGAGGGGTGGGCAGACACGTGAACATGTTCAACTTGCCAAAACATTGGGTGTCTCTAAACTGATTGTTGTTGTGAACAAGATGGATGACCCAACTGTGAACTGGTCGAAAGAAAGGTACGATGAAATAGAACAAAAAATGGTACCGTTCCTGAAAGCCTCTGGCTACAACACAAAGAAAGATGTTGTCTTCTTGCCTATATCTGGTCTAATGGGCAAAAATATGGACCAGAGGATGGGTCAAGAAATTTGTCCTTGGTGGAGTGGCCCTAGCTTTTTTGAAGTACTTGATTCTATTGAAATTCCGCCACGCGATCCTAATGGTCCATTCAGGATGCCAATTATTGATAAGTTCAAGGACATGGGAACTGTTGTTATGGGAAAAGTAGAATCTGGCAGCATTCGGGAGGGTGATTCCTTGGTTGTTATGCCGAACAAGGAACAAGTGAAAGTTGTTGCTATATATTGCGATGAGGATAAAGTTAAGCGTGCAGGACCGGGTGAGAATTTGAGAGTTCGTATTACTGGCATTGAAGATGAGGACATCCTTTCTGGTTTTGTTTTATCCAGCATTGTAAACCCTGTCCCTGCTGTTACCGAGTTTGTTGCCCAACTACAAATCCTTGAGCTGCTTGACAACGCTATTTTTACAGCTGGTTACAAGGCAATTCTCCACATCCATGCGGTTGTCGAGGAGTGTGAGATTATCGAATTGAAAAGCCAAATTGATCTCAAGACAAGGAAACCAATGAAAAAGAAAGTTTTGTTTGTGAAGAATGGTGCTGCTGTTGTGTGCCGCATCCAGGTTACGAATTCAATTTGTATTGAAAAATTCTCAGATTTCCCTCAACTTGGAAGATTCACTCTACGAACTGAAGGAAAAACAATTGCTGTTGGAAAGGTTACCGAGCTCTTAAGTTCTGTCTCAAGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

532

Amino Acids

59.24

Weight (kDa)

5.19

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 99 - 302 5.1e-49 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 343 - 409 1.6e-10 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 416 - 525 1e-23 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 421 - 523 1.5e-29 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 805
AccB1I GGYRCC 1 cut(s) 805
AccB7I CCANNNNNTGG 1 cut(s) 898
AccII CGCG 2 cut(s) 24, 975
AciI CCGC 5 cut(s) 22, 608, 968, 1346, 1459
AclWI GGATC 4 cut(s) 12, 13, 189, 971
AcsI RAATTY 7 cut(s) 100, 647, 909, 963, 1168, 1474, 1493
AcuI CTGAAG 2 cut(s) 291, 1557
AcvI CACGTG 1 cut(s) 683
AfaI GTAC 4 cut(s) 579, 782, 807, 948
AfeI AGCGCT 1 cut(s) 1594
AfiI CCNNNNNNNGG 4 cut(s) 866, 898, 1465, 1514
AflII CTTAAG 2 cut(s) 485, 1576
AflIII ACRYGT 2 cut(s) 680, 688
AjnI CCWGG 1 cut(s) 1464
Alw21I GWGCWC 2 cut(s) 536, 1575
Alw26I GTCTC 3 cut(s) 292, 721, 1591
AlwI GGATC 4 cut(s) 12, 13, 189, 971
AlwNI CAGNNNCTG 1 cut(s) 158
Aor51HI AGCGCT 1 cut(s) 1594
AoxI GGCC 1 cut(s) 928
ApeKI GCWGC 5 cut(s) 239, 254, 1059, 1291, 1445
ApoI RAATTY 7 cut(s) 100, 647, 909, 963, 1168, 1474, 1493
Asp700I GAANNNNTTC 2 cut(s) 147, 690
Asp718I GGTACC 1 cut(s) 805
AspLEI GCGC 2 cut(s) 26, 1595
AspS9I GGNCC 4 cut(s) 889, 929, 986, 1157
AsuC2I CCSGG 2 cut(s) 561, 1161
AsuHPI GGTGA 4 cut(s) 637, 656, 1085, 1175
AvaII GGWCC 3 cut(s) 889, 986, 1157
BaeGI GKGCMC 1 cut(s) 567
BanI GGYRCC 1 cut(s) 805
BanII GRGCYC 2 cut(s) 517, 1575
BbrPI CACGTG 1 cut(s) 683
BbsI GAAGAC 2 cut(s) 126, 844
Bbv12I GWGCWC 2 cut(s) 536, 1575
BbvI GCAGC 5 cut(s) 226, 241, 1071, 1278, 1432
BccI CCATC 2 cut(s) 739, 892
BcgI CGANNNNNNTGC 2 cut(s) 1250, 1284
BciT130I CCWGG 1 cut(s) 1466
BciVI GTATCC 1 cut(s) 457
BcnI CCSGG 2 cut(s) 561, 1161
BcoDI GTCTC 3 cut(s) 292, 721, 1591
BfaI CTAG 2 cut(s) 537, 933
BfoI RGCGCY 1 cut(s) 1596
BfrI CTTAAG 2 cut(s) 485, 1576
BfuI GTATCC 1 cut(s) 457
BisI GCNGC 6 cut(s) 240, 255, 1060, 1292, 1446, 1459
BlsI GCNGC 6 cut(s) 241, 256, 1061, 1293, 1447, 1460
BmcAI AGTACT 1 cut(s) 948
Bme1390I CCNGG 3 cut(s) 561, 1161, 1466
Bme18I GGWCC 3 cut(s) 889, 986, 1157
BmgT120I GGNCC 4 cut(s) 889, 929, 986, 1157
BmiI GGNNCC 1 cut(s) 807
BmrFI CCNGG 3 cut(s) 561, 1161, 1466
BmsI GCATC 5 cut(s) 322, 544, 608, 987, 1470
BpiI GAAGAC 2 cut(s) 126, 844
BpuEI CTTGAG 6 cut(s) 29, 228, 588, 1307, 1379, 1573
BpuMI CCSGG 2 cut(s) 561, 1161
BsaAI YACGTR 2 cut(s) 577, 683
BsaJI CCNNGG 3 cut(s) 560, 917, 1080
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 4 cut(s) 866, 898, 1465, 1514
Bse1I ACTGG 4 cut(s) 152, 206, 770, 1192
BseBI CCWGG 1 cut(s) 1466
BseDI CCNNGG 3 cut(s) 560, 917, 1080
BseGI GGATG 8 cut(s) 202, 391, 754, 903, 1002, 1206, 1338, 1461
BseLI CCNNNNNNNGG 4 cut(s) 866, 898, 1465, 1514
BseMII CTCAG 3 cut(s) 6, 168, 1512
BseNI ACTGG 4 cut(s) 152, 206, 770, 1192
BseRI GAGGAG 1 cut(s) 1370
BseSI GKGCMC 1 cut(s) 567
BseXI GCAGC 5 cut(s) 226, 241, 1071, 1278, 1432
BsgI GTGCAG 1 cut(s) 1173
Bsh1236I CGCG 2 cut(s) 24, 975
BshFI GGCC 1 cut(s) 930
BshNI GGYRCC 1 cut(s) 805
BsiHKAI GWGCWC 2 cut(s) 536, 1575
BsiSI CCGG 3 cut(s) 116, 560, 1160
BslFI GGGAC 1 cut(s) 1232
BslI CCNNNNNNNGG 4 cut(s) 866, 898, 1465, 1514
BsmAI GTCTC 3 cut(s) 292, 721, 1591
BsmFI GGGAC 1 cut(s) 1232
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 1 cut(s) 930
Bsp1286I GDGCHC 4 cut(s) 517, 536, 567, 1575
Bsp143I GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
BspACI CCGC 5 cut(s) 22, 608, 968, 1346, 1459
BspANI GGCC 1 cut(s) 930
BspCNI CTCAG 3 cut(s) 7, 169, 1511
BspFNI CGCG 2 cut(s) 24, 975
BspHI TCATGA 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 807
BspPI GGATC 4 cut(s) 12, 13, 189, 971
BspT107I GGYRCC 1 cut(s) 805
BspTI CTTAAG 2 cut(s) 485, 1576
BsrI ACTGG 4 cut(s) 152, 206, 770, 1192
BssECI CCNNGG 3 cut(s) 560, 917, 1080
BssMI GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
BssT1I CCWWGG 2 cut(s) 917, 1080
Bst2UI CCWGG 1 cut(s) 1466
Bst4CI ACNGT 4 cut(s) 499, 760, 810, 1033
Bst6I CTCTTC 1 cut(s) 471
BstAFI CTTAAG 2 cut(s) 485, 1576
BstBAI YACGTR 2 cut(s) 577, 683
BstC8I GCNNGC 1 cut(s) 1152
BstDEI CTNAG 4 cut(s) 15, 177, 371, 1498
BstEII GGTNACC 1 cut(s) 1564
BstF5I GGATG 8 cut(s) 202, 391, 754, 903, 1002, 1206, 1338, 1461
BstFNI CGCG 2 cut(s) 24, 975
BstH2I RGCGCY 1 cut(s) 1596
BstHHI GCGC 2 cut(s) 26, 1595
BstKTI GATC 6 cut(s) 7, 21, 184, 391, 979, 1393
BstMAI GTCTC 3 cut(s) 292, 721, 1591
BstMBI GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 605
BstNI CCWGG 1 cut(s) 1466
BstNSI RCATGY 2 cut(s) 329, 692
BstPI GGTNACC 1 cut(s) 1564
BstSCI CCNGG 3 cut(s) 559, 1159, 1464
BstSLI GKGCMC 1 cut(s) 567
BstSNI TACGTA 1 cut(s) 577
BstUI CGCG 2 cut(s) 24, 975
BstV1I GCAGC 5 cut(s) 226, 241, 1071, 1278, 1432
BstV2I GAAGAC 2 cut(s) 126, 844
BstX2I RGATCY 2 cut(s) 4, 18
BstXI CCANNNNNNTGG 1 cut(s) 709
BstYI RGATCY 2 cut(s) 4, 18
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 1146
BtsCI GGATG 8 cut(s) 202, 391, 754, 903, 1002, 1206, 1338, 1461
Cac8I GCNNGC 1 cut(s) 1152
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 1 cut(s) 184
CfoI GCGC 2 cut(s) 26, 1595
Cfr13I GGNCC 4 cut(s) 889, 929, 986, 1157
Csp6I GTAC 4 cut(s) 578, 781, 806, 947
CviAII CATG 6 cut(s) 89, 185, 326, 689, 1024, 1343
CviQI GTAC 4 cut(s) 578, 781, 806, 947
DdeI CTNAG 4 cut(s) 15, 177, 371, 1498
DpnI GATC 6 cut(s) 6, 20, 183, 390, 978, 1392
DpnII GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
EciI GGCGGA 1 cut(s) 957
Ecl136II GAGCTC 1 cut(s) 1573
Eco105I TACGTA 1 cut(s) 577
Eco130I CCWWGG 2 cut(s) 917, 1080
Eco24I GRGCYC 2 cut(s) 517, 1575
Eco47I GGWCC 3 cut(s) 889, 986, 1157
Eco47III AGCGCT 1 cut(s) 1594
Eco53kI GAGCTC 1 cut(s) 1573
Eco57I CTGAAG 2 cut(s) 291, 1557
Eco72I CACGTG 1 cut(s) 683
Eco91I GGTNACC 1 cut(s) 1564
EcoICRI GAGCTC 1 cut(s) 1573
EcoO65I GGTNACC 1 cut(s) 1564
EcoRI GAATTC 1 cut(s) 1474
EcoRII CCWGG 1 cut(s) 1464
EcoT14I CCWWGG 2 cut(s) 917, 1080
EcoT38I GRGCYC 2 cut(s) 517, 1575
ErhI CCWWGG 2 cut(s) 917, 1080
FaeI CATG 6 cut(s) 92, 188, 329, 692, 1027, 1346
FalI AAGNNNNNCTT 6 cut(s) 183, 215, 287, 319, 468, 500
FaqI GGGAC 1 cut(s) 1232
FatI CATG 6 cut(s) 88, 184, 325, 688, 1023, 1342
Fnu4HI GCNGC 6 cut(s) 240, 255, 1060, 1292, 1446, 1459
FokI GGATG 8 cut(s) 189, 398, 761, 910, 1009, 1193, 1325, 1448
FriOI GRGCYC 2 cut(s) 517, 1575
Fsp4HI GCNGC 6 cut(s) 240, 255, 1060, 1292, 1446, 1459
FspBI CTAG 2 cut(s) 537, 933
GlaI GCGC 2 cut(s) 25, 1594
GluI GCNGC 6 cut(s) 240, 255, 1060, 1292, 1446, 1459
HaeII RGCGCY 1 cut(s) 1596
HaeIII GGCC 1 cut(s) 930
HapII CCGG 3 cut(s) 116, 560, 1160
HhaI GCGC 2 cut(s) 26, 1595
Hin1II CATG 6 cut(s) 92, 188, 329, 692, 1027, 1346
Hin6I GCGC 2 cut(s) 24, 1593
HinP1I GCGC 2 cut(s) 24, 1593
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 8 cut(s) 38, 143, 361, 540, 953, 1052, 1076, 1521
HpaII CCGG 3 cut(s) 116, 560, 1160
HphI GGTGA 4 cut(s) 637, 656, 1085, 1175
Hpy166II GTNNAC 5 cut(s) 345, 686, 739, 763, 1240
Hpy188I TCNGA 2 cut(s) 178, 1501
Hpy8I GTNNAC 5 cut(s) 345, 686, 739, 763, 1240
HpyAV CCTTC 4 cut(s) 244, 406, 504, 1532
HpyCH4III ACNGT 4 cut(s) 499, 760, 810, 1033
HpyCH4IV ACGT 2 cut(s) 576, 682
HpyCH4V TGCA 3 cut(s) 139, 281, 1154
HpyF10VI GCNNNNNNNGC 1 cut(s) 605
HpyF3I CTNAG 4 cut(s) 15, 177, 371, 1498
HpySE526I ACGT 2 cut(s) 576, 682
Hsp92II CATG 6 cut(s) 92, 188, 329, 692, 1027, 1346
HspAI GCGC 2 cut(s) 24, 1593
KpnI GGTACC 1 cut(s) 809
Kzo9I GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
LmnI GCTCC 2 cut(s) 562, 596
Lsp1109I GCAGC 5 cut(s) 226, 241, 1071, 1278, 1432
LweI GCATC 5 cut(s) 322, 544, 608, 987, 1470
MaeI CTAG 2 cut(s) 537, 933
MaeII ACGT 2 cut(s) 576, 682
MaeIII GTNAC 5 cut(s) 572, 1255, 1319, 1468, 1564
MalI GATC 6 cut(s) 6, 20, 183, 390, 978, 1392
MboI GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
MboII GAAGA 8 cut(s) 131, 284, 485, 488, 844, 1208, 1447, 1530
MfeI CAATTG 2 cut(s) 348, 1548
MflI RGATCY 2 cut(s) 4, 18
MhlI GDGCHC 4 cut(s) 517, 536, 567, 1575
MmeI TCCRAC 2 cut(s) 487, 1537
MroXI GAANNNNTTC 2 cut(s) 147, 690
MseI TTAA 3 cut(s) 486, 1146, 1577
MslI CAYNNNNRTG 4 cut(s) 306, 330, 617, 1341
MspA1I CMGCKG 1 cut(s) 1316
MspCI CTTAAG 2 cut(s) 485, 1576
MspI CCGG 3 cut(s) 116, 560, 1160
MspR9I CCNGG 3 cut(s) 561, 1161, 1466
MunI CAATTG 2 cut(s) 348, 1548
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 1 cut(s) 1466
MvnI CGCG 2 cut(s) 24, 975
MwoI GCNNNNNNNGC 1 cut(s) 605
NciI CCSGG 2 cut(s) 561, 1161
NdeII GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
NlaIII CATG 6 cut(s) 92, 188, 329, 692, 1027, 1346
NlaIV GGNNCC 1 cut(s) 807
NspI RCATGY 2 cut(s) 329, 692
PagI TCATGA 1 cut(s) 184
PciI ACATGT 1 cut(s) 688
PctI GAATGC 1 cut(s) 1062
PdmI GAANNNNTTC 2 cut(s) 147, 690
PfeI GAWTC 8 cut(s) 38, 143, 361, 540, 953, 1052, 1076, 1521
PflMI CCANNNNNTGG 1 cut(s) 898
PkrI GCNGC 6 cut(s) 241, 256, 1061, 1293, 1447, 1460
PmaCI CACGTG 1 cut(s) 683
PmlI CACGTG 1 cut(s) 683
Ppu21I YACGTR 2 cut(s) 577, 683
PscI ACATGT 1 cut(s) 688
Psp124BI GAGCTC 1 cut(s) 1575
Psp6I CCWGG 1 cut(s) 1464
PspCI CACGTG 1 cut(s) 683
PspEI GGTNACC 1 cut(s) 1564
PspGI CCWGG 1 cut(s) 1464
PspN4I GGNNCC 1 cut(s) 807
PspPI GGNCC 4 cut(s) 889, 929, 986, 1157
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 2 cut(s) 4, 18
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 4 cut(s) 579, 782, 807, 948
RsaNI GTAC 4 cut(s) 578, 781, 806, 947
RseI CAYNNNNRTG 4 cut(s) 306, 330, 617, 1341
SacI GAGCTC 1 cut(s) 1575
SaqAI TTAA 3 cut(s) 486, 1146, 1577
SatI GCNGC 6 cut(s) 240, 255, 1060, 1292, 1446, 1459
Sau3AI GATC 6 cut(s) 4, 18, 181, 388, 976, 1390
Sau96I GGNCC 4 cut(s) 889, 929, 986, 1157
ScaI AGTACT 1 cut(s) 948
ScrFI CCNGG 3 cut(s) 561, 1161, 1466
SduI GDGCHC 4 cut(s) 517, 536, 567, 1575
SfaNI GCATC 5 cut(s) 322, 544, 608, 987, 1470
SinI GGWCC 3 cut(s) 889, 986, 1157
SmiMI CAYNNNNRTG 4 cut(s) 306, 330, 617, 1341
SmlI CTYRAG 8 cut(s) 8, 243, 485, 603, 1286, 1394, 1576, 1588
SmoI CTYRAG 8 cut(s) 8, 243, 485, 603, 1286, 1394, 1576, 1588
SnaBI TACGTA 1 cut(s) 577
SsiI CCGC 5 cut(s) 22, 608, 968, 1346, 1459
SspMI CTAG 2 cut(s) 537, 933
SstI GAGCTC 1 cut(s) 1575
StyD4I CCNGG 3 cut(s) 559, 1159, 1464
StyI CCWWGG 2 cut(s) 917, 1080
TaaI ACNGT 4 cut(s) 499, 760, 810, 1033
TaiI ACGT 2 cut(s) 579, 685
TaqI TCGA 5 cut(s) 391, 637, 770, 1353, 1371
TatI WGTACW 1 cut(s) 946
TauI GCSGC 1 cut(s) 1461
TfiI GAWTC 8 cut(s) 38, 143, 361, 540, 953, 1052, 1076, 1521
Tru1I TTAA 3 cut(s) 486, 1146, 1577
Tru9I TTAA 3 cut(s) 486, 1146, 1577
TseI GCWGC 5 cut(s) 239, 254, 1059, 1291, 1445
TspGWI ACGGA 1 cut(s) 542
Van91I CCANNNNNTGG 1 cut(s) 898
Vha464I CTTAAG 2 cut(s) 485, 1576
VpaK11BI GGWCC 3 cut(s) 889, 986, 1157
XapI RAATTY 7 cut(s) 100, 647, 909, 963, 1168, 1474, 1493
XceI RCATGY 2 cut(s) 329, 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 762
XmnI GAANNNNTTC 2 cut(s) 147, 690
XspI CTAG 2 cut(s) 537, 933
ZrmI AGTACT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.