Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0004512

Physical Location & Seq

Genomic Coordinates Reverse (-)

66125694 .. 66138211

Gene Structure 12518 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00274490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGATGCAGCCACGTAGCCCTAGTGATGGTAATGGGACCTCGAAATCTAGGGATATTGAGGAGGAAATCCGGTCGCTGCAGCTGGATTCAGCAGAAGATAATGGGGATGTAAATCCGGAAGCTGGAAATCTGGATGTCGAGAATTCTGATGATAAGATGGAGGAAGTTGATGATAAGATGGAGGAAGGTGATGATAATATGGAGGAAGGTTCCAGGGATGCCGTTGAGCCAGTGCAAAAAGTGAAGGAGAAGAAAGACGAACTTGATGAGGTGGAAATGAATAAGAAGAGGCACTTGAATGTTGTTTTTATTGGCCATGTGGATGCTGGAAAGTCAACAACAGGAGGTCAAATCCTTTTCCTTAGTGGTCAGGTTGATGATCGTACAATTCAAAAATATGAAAAAGAAGCGAAAGACAAAAGTAGGGAGAGCTGGTATATGGCATTTATCATGGATACCAATGAAGAAGAGAGGGCTAAGGGTAAAACTATTGAAGTTGGACGAGCACATTTTGAAACTGAGACAACTCGATTTACGATCTTGGATGCGCCAAGAATTCTGATTGCCCACGTCTTGGATCAGGGTCACAAAAGTTATGTACCAAATATGATCAGTGGTGCATCTCAGGCTGATATAGGAGTGCTGGTAATATCTGCCCGGAAAGGAGAATTTGAAACAGGGTATGAAAGAGGTGGACAGACCCGTGAACATGTTCAGCTTGCAAAAACTTTGGGAGTGTCTAAACTGCTTGTTGTTGTGAATAAAATGGATGATCTTACTGTGAACTGGTCAAAAGAAAGGTATGATGAAATTGAGTCGAAGATGGTACCATTCCTTAAGACATCAGGCTACAACGTGAAGAAAGATGTGCAGTTCATACCAATATCTGGTCTTCTTGGTACAAACATGAAAACTAGGTTGAGTAAAGAAACTTGTTCATGGTGGGATGGCCCCTGCCTTTTTGAAGCCCTTGACTCTATTGAAGTTCCTCTACGAGACCCTAAAGGTCCATTCAGCGTGATGCGTGATAGAGGCATTATGCCAGTAGTTTTCAGTGGGAGTCATGATGTACTGTCTTTATTAGTTGCCATACTGTATCAGTTGCCCATCATAGATAAATTCAAGGACATGGGAACTACTGTTATGGGGAAAGTGGAATCTGGTTCAGTGCGTGAAGGGGATTCACTGATTATCATGCCAAATAAGGCTCAAGTGAAAGTTATTGCTATTTTCATTGATGAAGATAGGGTCAAATATGCTGGACCTGGTGAAAATCTTCGAGTTAGGTTATCAGGTATCGACGACGAGGACATACTGTCAGGTTTTGTCTTGTCGAGTGTGGCAAATCCAATACCTGCTGTTACCGAGTTTTATGCGCAGTTGCAGATCCTTGAGTTGCTGGAAAATGCAATCTTTACACCTGGATACAAAGCTGTCCTGCATATTCACTCTGTTGTTGAGGAATGTGAGATTGTTGATCTGCTAGAGGAAATTGATATGAAGACTAAGAAACCTAAGAAGAAGAAGCCTCTTTTTGTGAAGAATGGTGCTGTTATTAAATGCCGGATTCAGGTGACTAGCTTGATATGCATGGAGAAGTTCTCCGATTTTCCCCAACTTGGAAGGTTTACTCTTCGTACTGAAGGTAAAACTATTGCTGTGGGAAAAGTAATTGATGCTCCATCCAAGAAAAATAGCTTAAAGTCCATAGATTTTAATCTTGTCTCAATTTCCAAAGCATTCTTCAAGGTGCAGAGCAGGGTTTCAGTCTTTCAGAGTTCAGAGAGACTTTGGAGTTTTTTCCAGAAAAACGACCCGAGAGAGATGGCCAGCTCTGCACATACAATGAATGGTGGGGATGGAAAGTACAGCTACTCTGTTCACTCCAATAATCAGCCAGATAGGCGATATTTTGCGGCTGGAGTTCCGGGATCTTTTCATGGAAGATTGTTTCCTAAAGCCTTCCTAGATTTTGTTTACTCTCAGTATGCTCTCCAATGGCTTTCTAGGGTACCACCAGAGATACTTGACTCCAACTCACCTGCATTCAACAAGGGGAGGATTATGTATGGAAATTCGGCTAGTGAAGTTGGTCAGGCATTTTCGGATCAGTATGCCAAGGACATGCAGTCTTTTTTTGATGCTCGAGCCCATGAGCTTGCTCCTGGAGGGTTATTGTTGCTCTTGATTCCAGGGCGTCCGGATGGAACTGTTCGGGCACAACATTCCGTACATCCGCTTTTTGAACCCATGGAGTCTAGTCTATCCGGTATGGTTAATCAGGGTTTAATAAGTGGAGATAAACTTGACTCTTTCAACTTGCCTATCTACTCTCCATCCATGGAAGAGTTAAGGAAGATAATAGAAAACAGCGAGTGCTTCGATGTATTAAGGTTGGAGATCCAATTACCTGAGAGGCCAACCCCTTTGCGTAGTGAGTATGCTAGATCAGGCTTTGAGAAAATTCTCACCAAACAGTTTGGGACTGAAATTATAGAACCACTTTTCGATCGATACTCCAAGAAAGTACAAGGTCGTTCTCCCATTACTTCTTGTGATGTGATTGAAGAAGCTTTTCCTATGAATGGAGGAGATGACCAATACAGCTATGGTAAAAACTCCAATCGTCAGAGGAAGGCAGCTGCCAATGTCAAGGCAATCCTGGTTGAGGCCATTCTTGAAAACCTTCAGATTGGTAATCTGTGTAGTACTACTAATTCTAACTCATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0002184 GO:0003674 GO:0003676 GO:0003723 GO:0003747 GO:0003824 GO:0003924 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006415 GO:0006464 GO:0006479 GO:0006518 GO:0006807 GO:0008079 GO:0008135 GO:0008150 GO:0008152 GO:0008213 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0018444 GO:0019538 GO:0022411 GO:0032259 GO:0032984 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0043043 GO:0043170 GO:0043412 GO:0043414 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

910

Amino Acids

101.74

Weight (kDa)

5.47

Isoelectric Point (pI)

43.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	96 - 319	9e-44	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	378 - 444	2.8e-09	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	451 - 558	3.9e-23	Elongation factor Tu C-terminal domain
GTP-eEF1A_C	PF22594	456 - 557	1.8e-29	GTP-eEF1A C-terminal domain-like
Methyltransf_7	PF03492	628 - 845	4.8e-63	SAM dependent carboxyl methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18070 AT1G18070 AT1G18070
fragaria_vesca	FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000
malus_domestica	MD06G1224200.v1.1 MD14G1233200.v1.1
prunus_persica	Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1
pyrus_communis	pycom06g19950
rosa_chinensis	RchiOBHm_Chr7g0178871
rosa_laevigata	RLG00000005398
rosa_multiflora	Rmu_sc0000888.1_g000002
rosa_roxburghii	Rroxscaffold_3G00274490
rosa_rugosa	Rorug06G0420300 Rorug06G0420300 Rorug06G0420400 Rorug06G0420500 Rorug06G0420500 Rorug06G0420500
rosa_samantha	Rh7AG019800 Rh7BG019300 Rh7CG019900 Rh7DG019800
rosa_wichuraiana	Rw7G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2050
Acc16I	TGCGCA	1 cut(s)	1377
Acc36I	ACCTGC	2 cut(s)	1363, 2050
Acc65I	GGTACC	2 cut(s)	826, 2011
AccB1I	GGYRCC	2 cut(s)	826, 2011
AccB7I	CCANNNNNTGG	2 cut(s)	574, 887
AccIII	TCCGGA	2 cut(s)	115, 2200
AciI	CCGC	2 cut(s)	1916, 2237
AclWI	GGATC	5 cut(s)	585, 1381, 1939, 2115, 2395
AcoI	YGGCCR	2 cut(s)	313, 1827
AcsI	RAATTY	6 cut(s)	142, 555, 668, 1118, 2074, 2463
AcuI	CTGAAG	2 cut(s)	1662, 2672
AcyI	GRCGYC	1 cut(s)	2197
AfiI	CCNNNNNNNGG	8 cut(s)	26, 122, 574, 887, 1570, 1619, 2585, 2668
AflII	CTTAAG	1 cut(s)	836
AflIII	ACRYGT	1 cut(s)	709
AhdI	GACNNNNNGTC	2 cut(s)	1315, 2128
AjiI	CACGTC	1 cut(s)	571
AjnI	CCWGG	6 cut(s)	212, 1264, 1420, 2164, 2191, 2661
AjuI	GAANNNNNNNTTGG	2 cut(s)	1727, 1759
Alw21I	GWGCWC	1 cut(s)	508
Alw26I	GTCTC	4 cut(s)	515, 990, 1729, 1780
AlwI	GGATC	5 cut(s)	585, 1381, 1939, 2115, 2395
Ama87I	CYCGRG	2 cut(s)	1816, 2145
Aor13HI	TCCGGA	2 cut(s)	115, 2200
AoxI	GGCC	5 cut(s)	313, 949, 1827, 2417, 2670
ApeKI	GCWGC	5 cut(s)	7, 76, 79, 2639, 2642
ApoI	RAATTY	6 cut(s)	142, 555, 668, 1118, 2074, 2463
Asp700I	GAANNNNTTC	4 cut(s)	711, 1275, 2574, 2685
Asp718I	GGTACC	2 cut(s)	826, 2011
AspLEI	GCGC	2 cut(s)	550, 1378
AspS9I	GGNCC	4 cut(s)	36, 950, 1007, 1262
AsuC2I	CCSGG	2 cut(s)	658, 1929
AsuHPI	GGTGA	5 cut(s)	200, 1280, 1585, 2031, 2461
AvaI	CYCGRG	2 cut(s)	1816, 2145
AvaII	GGWCC	3 cut(s)	36, 1007, 1262
BaeGI	GKGCMC	1 cut(s)	2221
BalI	TGGCCA	2 cut(s)	315, 1829
BanI	GGYRCC	2 cut(s)	826, 2011
BanII	GRGCYC	1 cut(s)	2152
BarI	GAAGNNNNNNTAC	2 cut(s)	975, 1007
BbsI	GAAGAC	2 cut(s)	884, 1508
Bbv12I	GWGCWC	1 cut(s)	508
BbvI	GCAGC	5 cut(s)	19, 63, 91, 2629, 2651
BceAI	ACGGC	1 cut(s)	206
BcgI	CGANNNNNNTGC	2 cut(s)	1355, 1389
BciT130I	CCWGG	6 cut(s)	214, 1266, 1422, 2166, 2193, 2663
BciVI	GTATCC	2 cut(s)	448, 1418
BclI	TGATCA	1 cut(s)	609
BcnI	CCSGG	2 cut(s)	658, 1929
BcoDI	GTCTC	4 cut(s)	515, 990, 1729, 1780
BfmI	CTRYAG	1 cut(s)	77
BfrI	CTTAAG	1 cut(s)	836
BfuAI	ACCTGC	2 cut(s)	1363, 2050
BfuI	GTATCC	2 cut(s)	448, 1418
BglI	GCCNNNNNGGC	1 cut(s)	1903
BisI	GCNGC	6 cut(s)	8, 77, 80, 1917, 2640, 2643
BlsI	GCNGC	6 cut(s)	9, 78, 81, 1918, 2641, 2644
BmcAI	AGTACT	1 cut(s)	2710
Bme1390I	CCNGG	8 cut(s)	214, 658, 1266, 1422, 1929, 2166, 2193, 2663
Bme18I	GGWCC	3 cut(s)	36, 1007, 1262
BmeRI	GACNNNNNGTC	2 cut(s)	1315, 2128
BmeT110I	CYCGRG	2 cut(s)	1816, 2145
BmgBI	CACGTC	1 cut(s)	571
BmgT120I	GGNCC	4 cut(s)	36, 950, 1007, 1262
BmiI	GGNNCC	5 cut(s)	37, 211, 828, 952, 2013
BmrFI	CCNGG	8 cut(s)	214, 658, 1266, 1422, 1929, 2166, 2193, 2663
BmsI	GCATC	7 cut(s)	208, 313, 535, 629, 1011, 1666, 2131
BpiI	GAAGAC	2 cut(s)	884, 1508
BplI	GAGNNNNNCTC	2 cut(s)	1586, 1618
BpmI	CTGGAG	2 cut(s)	1941, 2187
Bpu10I	CCTNAGC	1 cut(s)	477
BpuEI	CTTGAG	2 cut(s)	1194, 1412
BpuMI	CCSGG	2 cut(s)	658, 1929
Bsa29I	ATCGAT	1 cut(s)	2512
BsaAI	YACGTR	1 cut(s)	14
BsaBI	GATNNNNATC	2 cut(s)	111, 1716
BsaHI	GRCGYC	1 cut(s)	2197
BsaI	GGTCTC	1 cut(s)	990
BsaJI	CCNNGG	5 cut(s)	213, 2118, 2192, 2250, 2340
BsaWI	WCCGGW	4 cut(s)	69, 115, 2200, 2267
BsaXI	ACNNNNNCTCC	4 cut(s)	419, 449, 630, 660
Bsc4I	CCNNNNNNNGG	8 cut(s)	26, 122, 574, 887, 1570, 1619, 2585, 2668
Bse1I	ACTGG	3 cut(s)	230, 791, 1043
Bse8I	GATNNNNATC	2 cut(s)	111, 1716
BseAI	TCCGGA	2 cut(s)	115, 2200
BseBI	CCWGG	6 cut(s)	214, 1266, 1422, 2166, 2193, 2663
BseCI	ATCGAT	1 cut(s)	2512
BseDI	CCNNGG	5 cut(s)	213, 2118, 2192, 2250, 2340
BseJI	GATNNNNATC	2 cut(s)	111, 1716
BseLI	CCNNNNNNNGG	8 cut(s)	26, 122, 574, 887, 1570, 1619, 2585, 2668
BseMII	CTCAG	4 cut(s)	510, 638, 1997, 2403
BseNI	ACTGG	3 cut(s)	230, 791, 1043
BseRI	GAGGAG	2 cut(s)	74, 2604
BseSI	GKGCMC	1 cut(s)	2221
BseXI	GCAGC	5 cut(s)	19, 63, 91, 2629, 2651
BsgI	GTGCAG	3 cut(s)	890, 1772, 1821
Bsh1285I	CGRYCG	2 cut(s)	74, 2512
BshFI	GGCC	5 cut(s)	315, 951, 1829, 2419, 2672
BshNI	GGYRCC	2 cut(s)	826, 2011
BshVI	ATCGAT	1 cut(s)	2512
BsiEI	CGRYCG	2 cut(s)	74, 2512
BsiHKAI	GWGCWC	1 cut(s)	508
BsiHKCI	CYCGRG	2 cut(s)	1816, 2145
BsiSI	CCGG	7 cut(s)	70, 116, 658, 1564, 1928, 2201, 2268
BslFI	GGGAC	2 cut(s)	49, 2497
BslI	CCNNNNNNNGG	8 cut(s)	26, 122, 574, 887, 1570, 1619, 2585, 2668
BsmAI	GTCTC	4 cut(s)	515, 990, 1729, 1780
BsmFI	GGGAC	2 cut(s)	49, 2497
BsmI	GAATGC	2 cut(s)	1739, 2045
BsnI	GGCC	5 cut(s)	315, 951, 1829, 2419, 2672
Bso31I	GGTCTC	1 cut(s)	990
BsoBI	CYCGRG	2 cut(s)	1816, 2145
Bsp1286I	GDGCHC	3 cut(s)	508, 2152, 2221
Bsp13I	TCCGGA	2 cut(s)	115, 2200
Bsp19I	CCATGG	2 cut(s)	2250, 2340
BspACI	CCGC	2 cut(s)	1916, 2237
BspANI	GGCC	5 cut(s)	315, 951, 1829, 2419, 2672
BspCNI	CTCAG	4 cut(s)	511, 637, 1996, 2404
BspDI	ATCGAT	1 cut(s)	2512
BspEI	TCCGGA	2 cut(s)	115, 2200
BspHI	TCATGA	1 cut(s)	1063
BspLI	GGNNCC	5 cut(s)	37, 211, 828, 952, 2013
BspMAI	CTGCAG	1 cut(s)	81
BspMI	ACCTGC	2 cut(s)	1363, 2050
BspPI	GGATC	5 cut(s)	585, 1381, 1939, 2115, 2395
BspT107I	GGYRCC	2 cut(s)	826, 2011
BspTI	CTTAAG	1 cut(s)	836
BspTNI	GGTCTC	1 cut(s)	990
BsrI	ACTGG	3 cut(s)	230, 791, 1043
BssECI	CCNNGG	5 cut(s)	213, 2118, 2192, 2250, 2340
BssNI	GRCGYC	1 cut(s)	2197
BssT1I	CCWWGG	3 cut(s)	2118, 2250, 2340
Bst2UI	CCWGG	6 cut(s)	214, 1266, 1422, 2166, 2193, 2663
Bst4CI	ACNGT	7 cut(s)	781, 1074, 1095, 1141, 1317, 2212, 2478
Bst6I	CTCTTC	4 cut(s)	281, 462, 1638, 2340
BstACI	GRCGYC	1 cut(s)	2197
BstAFI	CTTAAG	1 cut(s)	836
BstBAI	YACGTR	1 cut(s)	14
BstC8I	GCNNGC	3 cut(s)	720, 1831, 2160
BstDEI	CTNAG	8 cut(s)	362, 477, 519, 624, 1506, 1515, 1983, 2412
BstDSI	CCRYGG	2 cut(s)	2250, 2340
BstENI	CCTNNNNNAGG	1 cut(s)	2666
BstHHI	GCGC	2 cut(s)	550, 1378
BstMAI	GTCTC	4 cut(s)	515, 990, 1729, 1780
BstMCI	CGRYCG	2 cut(s)	74, 2512
BstMWI	GCNNNNNNNGC	4 cut(s)	626, 1587, 1835, 1903
BstNI	CCWGG	6 cut(s)	214, 1266, 1422, 2166, 2193, 2663
BstNSI	RCATGY	2 cut(s)	713, 2128
BstSCI	CCNGG	8 cut(s)	212, 656, 1264, 1420, 1927, 2164, 2191, 2661
BstSFI	CTRYAG	1 cut(s)	77
BstSLI	GKGCMC	1 cut(s)	2221
BstV1I	GCAGC	5 cut(s)	19, 63, 91, 2629, 2651
BstV2I	GAAGAC	2 cut(s)	884, 1508
BstX2I	RGATCY	3 cut(s)	1386, 1931, 2400
BstYI	RGATCY	3 cut(s)	1386, 1931, 2400
Bsu15I	ATCGAT	1 cut(s)	2512
BsuI	GTATCC	2 cut(s)	448, 1418
BsuRI	GGCC	5 cut(s)	315, 951, 1829, 2419, 2672
BsuTUI	ATCGAT	1 cut(s)	2512
BtgI	CCRYGG	2 cut(s)	2250, 2340
BtrI	CACGTC	1 cut(s)	571
BtsIMutI	CAGTG	5 cut(s)	237, 619, 1060, 1173, 1184
BveI	ACCTGC	2 cut(s)	1363, 2050
Cac8I	GCNNGC	3 cut(s)	720, 1831, 2160
CciI	TCATGA	1 cut(s)	1063
CfoI	GCGC	2 cut(s)	550, 1378
Cfr13I	GGNCC	4 cut(s)	36, 950, 1007, 1262
ClaI	ATCGAT	1 cut(s)	2512
CseI	GACGC	1 cut(s)	2186
CsiI	ACCWGGT	1 cut(s)	1264
DdeI	CTNAG	8 cut(s)	362, 477, 519, 624, 1506, 1515, 1983, 2412
DriI	GACNNNNNGTC	2 cut(s)	1315, 2128
EaeI	YGGCCR	2 cut(s)	313, 1827
Eam1104I	CTCTTC	4 cut(s)	281, 462, 1638, 2340
Eam1105I	GACNNNNNGTC	2 cut(s)	1315, 2128
EarI	CTCTTC	4 cut(s)	281, 462, 1638, 2340
Eco130I	CCWWGG	3 cut(s)	2118, 2250, 2340
Eco24I	GRGCYC	1 cut(s)	2152
Eco31I	GGTCTC	1 cut(s)	990
Eco47I	GGWCC	3 cut(s)	36, 1007, 1262
Eco57I	CTGAAG	2 cut(s)	1662, 2672
Eco88I	CYCGRG	2 cut(s)	1816, 2145
EcoNI	CCTNNNNNAGG	1 cut(s)	2666
EcoO109I	RGGNCCY	1 cut(s)	36
EcoRI	GAATTC	2 cut(s)	142, 555
EcoRII	CCWGG	6 cut(s)	212, 1264, 1420, 2164, 2191, 2661
EcoT14I	CCWWGG	3 cut(s)	2118, 2250, 2340
EcoT22I	ATGCAT	1 cut(s)	1592
EcoT38I	GRGCYC	1 cut(s)	2152
ErhI	CCWWGG	3 cut(s)	2118, 2250, 2340
FalI	AAGNNNNNCTT	4 cut(s)	246, 278, 278, 310
FaqI	GGGAC	2 cut(s)	49, 2497
FbaI	TGATCA	1 cut(s)	609
Fnu4HI	GCNGC	6 cut(s)	8, 77, 80, 1917, 2640, 2643
FriOI	GRGCYC	1 cut(s)	2152
Fsp4HI	GCNGC	6 cut(s)	8, 77, 80, 1917, 2640, 2643
FspI	TGCGCA	1 cut(s)	1377
GlaI	GCGC	2 cut(s)	549, 1377
GluI	GCNGC	6 cut(s)	8, 77, 80, 1917, 2640, 2643
GsuI	CTGGAG	2 cut(s)	1941, 2187
HaeIII	GGCC	5 cut(s)	315, 951, 1829, 2419, 2672
HapII	CCGG	7 cut(s)	70, 116, 658, 1564, 1928, 2201, 2268
HgaI	GACGC	1 cut(s)	2186
HhaI	GCGC	2 cut(s)	550, 1378
Hin1I	GRCGYC	1 cut(s)	2197
Hin6I	GCGC	2 cut(s)	548, 1376
HinP1I	GCGC	2 cut(s)	548, 1376
HincII	GTYRAC	1 cut(s)	336
HindII	GTYRAC	1 cut(s)	336
HindIII	AAGCTT	1 cut(s)	2571
HpaII	CCGG	7 cut(s)	70, 116, 658, 1564, 1928, 2201, 2268
HphI	GGTGA	5 cut(s)	200, 1280, 1585, 2031, 2461
Hpy166II	GTNNAC	7 cut(s)	336, 695, 707, 784, 1629, 1882, 1978
Hpy188I	TCNGA	9 cut(s)	148, 561, 1606, 1776, 1783, 2107, 2631, 2691, 2732
Hpy188III	TCNNGA	8 cut(s)	116, 131, 139, 1064, 1804, 2185, 2201, 2678
Hpy8I	GTNNAC	7 cut(s)	336, 695, 707, 784, 1629, 1882, 1978
Hpy99I	CGWCG	2 cut(s)	1304, 1307
HpyAV	CCTTC	9 cut(s)	179, 200, 238, 1169, 1617, 1637, 1972, 2629, 2696
HpyCH4III	ACNGT	7 cut(s)	781, 1074, 1095, 1141, 1317, 2212, 2478
HpyCH4IV	ACGT	3 cut(s)	13, 570, 855
HpyF10VI	GCNNNNNNNGC	4 cut(s)	626, 1587, 1835, 1903
HpyF3I	CTNAG	8 cut(s)	362, 477, 519, 624, 1506, 1515, 1983, 2412
HpySE526I	ACGT	3 cut(s)	13, 570, 855
Hsp92I	GRCGYC	1 cut(s)	2197
HspAI	GCGC	2 cut(s)	548, 1376
Kpn2I	TCCGGA	2 cut(s)	115, 2200
KpnI	GGTACC	2 cut(s)	830, 2015
Ksp22I	TGATCA	1 cut(s)	609
LmnI	GCTCC	2 cut(s)	1684, 2167
Lsp1109I	GCAGC	5 cut(s)	19, 63, 91, 2629, 2651
LweI	GCATC	7 cut(s)	208, 313, 535, 629, 1011, 1666, 2131
MabI	ACCWGGT	1 cut(s)	1264
MaeII	ACGT	3 cut(s)	13, 570, 855
MaeIII	GTNAC	3 cut(s)	584, 1360, 1573
MflI	RGATCY	3 cut(s)	1386, 1931, 2400
MhlI	GDGCHC	3 cut(s)	508, 2152, 2221
MlsI	TGGCCA	2 cut(s)	315, 1829
MluNI	TGGCCA	2 cut(s)	315, 1829
MlyI	GAGTC	6 cut(s)	824, 968, 1069, 2024, 2264, 2303
MmeI	TCCRAC	3 cut(s)	478, 2058, 2376
Mox20I	TGGCCA	2 cut(s)	315, 1829
Mph1103I	ATGCAT	1 cut(s)	1592
MroI	TCCGGA	2 cut(s)	115, 2200
MroXI	GAANNNNTTC	4 cut(s)	711, 1275, 2574, 2685
MscI	TGGCCA	2 cut(s)	315, 1829
MseI	TTAA	8 cut(s)	837, 1557, 1700, 1716, 2277, 2288, 2351, 2390
MslI	CAYNNNNRTG	2 cut(s)	297, 321
Msp20I	TGGCCA	2 cut(s)	315, 1829
MspA1I	CMGCKG	2 cut(s)	82, 2642
MspCI	CTTAAG	1 cut(s)	836
MspI	CCGG	7 cut(s)	70, 116, 658, 1564, 1928, 2201, 2268
MspR9I	CCNGG	8 cut(s)	214, 658, 1266, 1422, 1929, 2166, 2193, 2663
Mva1269I	GAATGC	2 cut(s)	1739, 2045
MvaI	CCWGG	6 cut(s)	214, 1266, 1422, 2166, 2193, 2663
MwoI	GCNNNNNNNGC	4 cut(s)	626, 1587, 1835, 1903
NciI	CCSGG	2 cut(s)	658, 1929
NcoI	CCATGG	2 cut(s)	2250, 2340
NlaIV	GGNNCC	5 cut(s)	37, 211, 828, 952, 2013
NmuCI	GTSAC	2 cut(s)	584, 1573
NsbI	TGCGCA	1 cut(s)	1377
NsiI	ATGCAT	1 cut(s)	1592
NspI	RCATGY	2 cut(s)	713, 2128
PaeR7I	CTCGAG	1 cut(s)	2145
PagI	TCATGA	1 cut(s)	1063
PaqCI	CACCTGC	1 cut(s)	2050
PciI	ACATGT	1 cut(s)	709
PctI	GAATGC	2 cut(s)	1739, 2045
PdmI	GAANNNNTTC	4 cut(s)	711, 1275, 2574, 2685
PfeI	GAWTC	5 cut(s)	86, 1157, 1181, 1567, 2188
PflMI	CCANNNNNTGG	2 cut(s)	574, 887
PfoI	TCCNGGA	2 cut(s)	1927, 2164
PkrI	GCNGC	6 cut(s)	9, 78, 81, 1918, 2641, 2644
Ple19I	CGATCG	1 cut(s)	2512
PleI	GAGTC	6 cut(s)	823, 968, 1068, 2024, 2263, 2303
PpsI	GAGTC	6 cut(s)	823, 968, 1068, 2024, 2263, 2303
Ppu21I	YACGTR	1 cut(s)	14
PpuMI	RGGWCCY	1 cut(s)	36
PscI	ACATGT	1 cut(s)	709
Psp5II	RGGWCCY	1 cut(s)	36
Psp6I	CCWGG	6 cut(s)	212, 1264, 1420, 2164, 2191, 2661
PspGI	CCWGG	6 cut(s)	212, 1264, 1420, 2164, 2191, 2661
PspN4I	GGNNCC	5 cut(s)	37, 211, 828, 952, 2013
PspPI	GGNCC	4 cut(s)	36, 950, 1007, 1262
PspPPI	RGGWCCY	1 cut(s)	36
PspXI	VCTCGAGB	1 cut(s)	2145
PstI	CTGCAG	1 cut(s)	81
PsuI	RGATCY	3 cut(s)	1386, 1931, 2400
PvuI	CGATCG	1 cut(s)	2512
PvuII	CAGCTG	2 cut(s)	82, 2642
RseI	CAYNNNNRTG	2 cut(s)	297, 321
SaqAI	TTAA	8 cut(s)	837, 1557, 1700, 1716, 2277, 2288, 2351, 2390
SatI	GCNGC	6 cut(s)	8, 77, 80, 1917, 2640, 2643
Sau96I	GGNCC	4 cut(s)	36, 950, 1007, 1262
ScaI	AGTACT	1 cut(s)	2710
SchI	GAGTC	6 cut(s)	824, 968, 1069, 2024, 2264, 2303
ScrFI	CCNGG	8 cut(s)	214, 658, 1266, 1422, 1929, 2166, 2193, 2663
SduI	GDGCHC	3 cut(s)	508, 2152, 2221
SexAI	ACCWGGT	1 cut(s)	1264
SfaNI	GCATC	7 cut(s)	208, 313, 535, 629, 1011, 1666, 2131
SfcI	CTRYAG	1 cut(s)	77
Sfr274I	CTCGAG	1 cut(s)	2145
SinI	GGWCC	3 cut(s)	36, 1007, 1262
SlaI	CTCGAG	1 cut(s)	2145
SmiMI	CAYNNNNRTG	2 cut(s)	297, 321
SmlI	CTYRAG	4 cut(s)	836, 1209, 1391, 2145
SmoI	CTYRAG	4 cut(s)	836, 1209, 1391, 2145
SsiI	CCGC	2 cut(s)	1916, 2237
StyD4I	CCNGG	8 cut(s)	212, 656, 1264, 1420, 1927, 2164, 2191, 2661
StyI	CCWWGG	3 cut(s)	2118, 2250, 2340
TaaI	ACNGT	7 cut(s)	781, 1074, 1095, 1141, 1317, 2212, 2478
TaiI	ACGT	3 cut(s)	16, 573, 858
TatI	WGTACW	4 cut(s)	1069, 1866, 2527, 2708
TauI	GCSGC	1 cut(s)	1919
TfiI	GAWTC	5 cut(s)	86, 1157, 1181, 1567, 2188
Tru1I	TTAA	8 cut(s)	837, 1557, 1700, 1716, 2277, 2288, 2351, 2390
Tru9I	TTAA	8 cut(s)	837, 1557, 1700, 1716, 2277, 2288, 2351, 2390
TscAI	CASTG	5 cut(s)	237, 619, 1060, 1173, 1191
TseFI	GTSAC	2 cut(s)	584, 1573
TseI	GCWGC	5 cut(s)	7, 76, 79, 2639, 2642
Tsp45I	GTSAC	2 cut(s)	584, 1573
TspGWI	ACGGA	1 cut(s)	2218
TspRI	CASTG	5 cut(s)	237, 619, 1060, 1173, 1191
Van91I	CCANNNNNTGG	2 cut(s)	574, 887
Vha464I	CTTAAG	1 cut(s)	836
VpaK11BI	GGWCC	3 cut(s)	36, 1007, 1262
XagI	CCTNNNNNAGG	1 cut(s)	2666
XapI	RAATTY	6 cut(s)	142, 555, 668, 1118, 2074, 2463
XceI	RCATGY	2 cut(s)	713, 2128
XcmI	CCANNNNNNNNNTGG	2 cut(s)	323, 2606
XhoI	CTCGAG	1 cut(s)	2145
XmnI	GAANNNNTTC	4 cut(s)	711, 1275, 2574, 2685
ZrmI	AGTACT	1 cut(s)	2710
Zsp2I	ATGCAT	1 cut(s)	1592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.