Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004512

Physical Location & Seq

Genomic Coordinates Reverse (-)

31338198 .. 31345056

Gene Structure 6859 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1233200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGATATCGAGGAGGAAATCCGATCGTTACAGCTTGATTCGGAAGAAGATAATGGGGCTGTTAAGCCAGACGAAGCAATGCCGGAAGTAGAGAACGAGAGTAAGATGGAGGAAGATCCCAGGGAAGAGATTCATACGCAGTCTGATCAGTCAGTTGTAGTTGAGCATAAAGGGAAAGCGAAGGAAATCCCAGAGCCTGAAGAAGAACATGATGACATTGAATTAGATAGGAAGAGGCACTTGAATGTTGTTTTTATTGGCCATGTTGATGCTGGAAAGTCCACAACAGGAGGCCAAATTCTTTTCCTTAGCGGTCAGGTTGATGATCGTACGATCCAAAAATATGAAAAAGAAGCAAAGGACAAAAGTAGGGAAAGCTGGTATATGGCATATATTATGGATACCAATGAGGAAGAGAGAGAGAAGGGTAAAACAGTTGAAGTGGGGCGGGCCCACTTTGAAACCGAAACATCAAGATTTACGATTTTGGATGCACCCGGCCACAAAAGTTATGTTCCAAATATGATCAGTGGTGCATCTCAAGCTGATGTAGGAGTACTGGTAATTTCTGCTCGGAAAGGAGAATTTGAAACTGGGTATGAGAGGGGTGGGCAGACCCGTGAACACGTTCAACTTGCTAAGACATTGGGTGTGTCTAAGTTGCTTGTTGTTGTGAATAAAATGGATGATCCCACTGTGAACTGGTCAAAAGAAAGGTATGATGAAATTGGGTCAAAGATGGTGCCATTCCTTAAAGCATCAGGCTACAATGTGAAAAAAGATGTGCAATTCCTGCCTATATCTGGCCTGATTGGTTTAAATATAAAAACCAGATTGGATAAAAGTGTTTGTTCGTGGTGGGATGGTCCCTGCCTTTTCGAAGTCCTTGATTCTATTGAAGTTCCTCTACGAGATCCTAAAGGTTCATTCAGGATGCCTATCATAGACAGATTCAAGGACATGGGAACAGTTGTTATGGGCAAAGTGGAATCTGGGTCAGTGCGTGAAGGGGATTCGTTGGTTGTCATGCCAAATAAGGTTCCAGTGAAGGCTGTTGCTCTATACATTGATGATGATAGGGTGAAACGTGCGGGCCCCGGTGAAAATCTACGGGTTAGAATATCGGGCATTGAGGATGAAGATATATTGTCAGGCTTTGTCTTGTCGAGTGTTGCAAATCCAATACCTGCTGTAACAGAATTTTTCGCCCAGTTGCAGATCCTTGAACTGCTGGACAATGCAATCTTGACGGCTGGCTATAAAGCTGTCCTGCATATTCACTCGGTTGTTGAAGAATGTGAGATTATTGAGCTTGTAAGTCAAATGGATCCGAAGACGAAGAAACCTATGAAAAAGCATATTCGTTTTGTCAAGAATGGTGCCGTTGTTGTGTGCAAGATTCAGGTCGGTAACATGATCTGCATCGAGAAGTTTGCAGACTTTCCACAACTTGGAAGGTTTACTCTTCGTACCGAAGGAAAAACTATTGCAGTTGGAAAAGTGATTGATCAACCTTCAAATTACCGCAACAGATCAAAGCTGGGTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0002184 GO:0003674 GO:0003676 GO:0003723 GO:0003747 GO:0003824 GO:0003924 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006415 GO:0006464 GO:0006479 GO:0006518 GO:0006807 GO:0008079 GO:0008135 GO:0008150 GO:0008152 GO:0008213 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0018444 GO:0019538 GO:0022411 GO:0032259 GO:0032984 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0043043 GO:0043170 GO:0043412 GO:0043414 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

517

Amino Acids

57.8

Weight (kDa)

5.32

Isoelectric Point (pI)

45.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	78 - 280	6.1e-49	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	322 - 388	2.5e-09	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	395 - 502	2.8e-22	Elongation factor Tu C-terminal domain
GTP-eEF1A_C	PF22594	401 - 501	2.7e-29	GTP-eEF1A C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18070 AT1G18070 AT1G18070
fragaria_vesca	FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000
malus_domestica	MD06G1224200.v1.1 MD14G1233200.v1.1
prunus_persica	Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1
pyrus_communis	pycom06g19950
rosa_chinensis	RchiOBHm_Chr7g0178871
rosa_laevigata	RLG00000005398
rosa_multiflora	Rmu_sc0000888.1_g000002
rosa_roxburghii	Rroxscaffold_3G00274490
rosa_rugosa	Rorug06G0420300 Rorug06G0420300 Rorug06G0420400 Rorug06G0420500 Rorug06G0420500 Rorug06G0420500
rosa_samantha	Rh7AG019800 Rh7BG019300 Rh7CG019900 Rh7DG019800
rosa_wichuraiana	Rw7G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1195
AccB1I	GGYRCC	2 cut(s)	742, 1379
AccB7I	CCANNNNNTGG	1 cut(s)	1451
AciI	CCGC	4 cut(s)	312, 448, 1091, 1525
AclWI	GGATC	7 cut(s)	110, 328, 683, 908, 1213, 1322, 1335
AcoI	YGGCCR	2 cut(s)	259, 499
AcsI	RAATTY	3 cut(s)	297, 584, 1199
AcuI	CTGAAG	1 cut(s)	219
AfaI	GTAC	3 cut(s)	331, 558, 1471
AfiI	CCNNNNNNNGG	2 cut(s)	803, 1451
AflIII	ACRYGT	1 cut(s)	625
AjnI	CCWGG	1 cut(s)	119
AluBI	AGCT	6 cut(s)	34, 378, 545, 1265, 1312, 1540
AluI	AGCT	6 cut(s)	34, 378, 545, 1265, 1312, 1540
AlwI	GGATC	7 cut(s)	110, 328, 683, 908, 1213, 1322, 1335
AlwNI	CAGNNNCTG	1 cut(s)	197
AoxI	GGCC	6 cut(s)	259, 292, 450, 499, 805, 1093
ApaI	GGGCCC	2 cut(s)	454, 1097
ApoI	RAATTY	3 cut(s)	297, 584, 1199
Asp700I	GAANNNNTTC	2 cut(s)	129, 627
AspS9I	GGNCC	5 cut(s)	450, 451, 866, 1093, 1094
AsuC2I	CCSGG	2 cut(s)	498, 1098
AsuHPI	GGTGA	2 cut(s)	1093, 1112
AsuII	TTCGAA	1 cut(s)	879
AvaII	GGWCC	1 cut(s)	866
BaeGI	GKGCMC	2 cut(s)	454, 1097
BalI	TGGCCA	1 cut(s)	261
BamHI	GGATCC	1 cut(s)	1327
BanI	GGYRCC	2 cut(s)	742, 1379
BanII	GRGCYC	2 cut(s)	454, 1097
BarI	GAAGNNNNNNTAC	2 cut(s)	891, 923
BbsI	GAAGAC	1 cut(s)	1340
BccI	CCATC	3 cut(s)	100, 733, 857
BceAI	ACGGC	2 cut(s)	1266, 1367
BcgI	CGANNNNNNTGC	2 cut(s)	1415, 1449
BciT130I	CCWGG	1 cut(s)	121
BciVI	GTATCC	1 cut(s)	394
BclI	TGATCA	3 cut(s)	145, 525, 1507
BcnI	CCSGG	2 cut(s)	498, 1098
BfuAI	ACCTGC	1 cut(s)	1195
BfuI	GTATCC	1 cut(s)	394
BmcAI	AGTACT	1 cut(s)	558
Bme1390I	CCNGG	3 cut(s)	121, 498, 1098
Bme18I	GGWCC	1 cut(s)	866
BmgT120I	GGNCC	5 cut(s)	450, 451, 866, 1093, 1094
BmiI	GGNNCC	8 cut(s)	452, 744, 868, 1041, 1095, 1096, 1329, 1381
BmrFI	CCNGG	3 cut(s)	121, 498, 1098
BmrI	ACTGGG	2 cut(s)	603, 1204
BmsI	GCATC	6 cut(s)	259, 481, 545, 767, 924, 1431
BmuI	ACTGGG	2 cut(s)	603, 1204
BpiI	GAAGAC	1 cut(s)	1340
Bpu10I	CCTNAGC	1 cut(s)	308
Bpu14I	TTCGAA	1 cut(s)	879
BpuEI	CTTGAG	1 cut(s)	525
BpuMI	CCSGG	2 cut(s)	498, 1098
BsaBI	GATNNNNATC	2 cut(s)	938, 1421
BsaJI	CCNNGG	3 cut(s)	119, 120, 1096
BsaXI	ACNNNNNCTCC	2 cut(s)	546, 576
Bsc4I	CCNNNNNNNGG	2 cut(s)	803, 1451
Bse1I	ACTGG	5 cut(s)	564, 598, 707, 1043, 1210
Bse3DI	GCAATG	1 cut(s)	84
Bse8I	GATNNNNATC	2 cut(s)	938, 1421
BseBI	CCWGG	1 cut(s)	121
BseDI	CCNNGG	3 cut(s)	119, 120, 1096
BseGI	GGATG	5 cut(s)	496, 691, 868, 939, 1141
BseJI	GATNNNNATC	2 cut(s)	938, 1421
BseLI	CCNNNNNNNGG	2 cut(s)	803, 1451
BseMI	GCAATG	1 cut(s)	84
BseNI	ACTGG	5 cut(s)	564, 598, 707, 1043, 1210
BseRI	GAGGAG	1 cut(s)	26
BseSI	GKGCMC	2 cut(s)	454, 1097
BseYI	CCCAGC	1 cut(s)	1540
Bsh1285I	CGRYCG	1 cut(s)	26
BshFI	GGCC	6 cut(s)	261, 294, 452, 501, 807, 1095
BshNI	GGYRCC	2 cut(s)	742, 1379
BsiEI	CGRYCG	1 cut(s)	26
BsiSI	CCGG	3 cut(s)	83, 498, 1098
BsiWI	CGTACG	1 cut(s)	329
BslFI	GGGAC	1 cut(s)	852
BslI	CCNNNNNNNGG	2 cut(s)	803, 1451
BsmFI	GGGAC	1 cut(s)	852
BsnI	GGCC	6 cut(s)	261, 294, 452, 501, 807, 1095
Bsp119I	TTCGAA	1 cut(s)	879
Bsp120I	GGGCCC	2 cut(s)	450, 1093
Bsp1286I	GDGCHC	2 cut(s)	454, 1097
BspACI	CCGC	4 cut(s)	312, 448, 1091, 1525
BspANI	GGCC	6 cut(s)	261, 294, 452, 501, 807, 1095
BspLI	GGNNCC	8 cut(s)	452, 744, 868, 1041, 1095, 1096, 1329, 1381
BspMI	ACCTGC	1 cut(s)	1195
BspPI	GGATC	7 cut(s)	110, 328, 683, 908, 1213, 1322, 1335
BspT104I	TTCGAA	1 cut(s)	879
BspT107I	GGYRCC	2 cut(s)	742, 1379
BsrDI	GCAATG	1 cut(s)	84
BsrI	ACTGG	5 cut(s)	564, 598, 707, 1043, 1210
BssECI	CCNNGG	3 cut(s)	119, 120, 1096
Bst2UI	CCWGG	1 cut(s)	121
Bst4CI	ACNGT	3 cut(s)	436, 697, 970
Bst6I	CTCTTC	4 cut(s)	120, 227, 408, 1470
BstAPI	GCANNNNNTGC	1 cut(s)	793
BstBI	TTCGAA	1 cut(s)	879
BstC8I	GCNNGC	3 cut(s)	450, 1093, 1255
BstDEI	CTNAG	3 cut(s)	308, 639, 657
BstF5I	GGATG	5 cut(s)	496, 691, 868, 939, 1141
BstMCI	CGRYCG	1 cut(s)	26
BstMWI	GCNNNNNNNGC	2 cut(s)	542, 793
BstNI	CCWGG	1 cut(s)	121
BstSCI	CCNGG	3 cut(s)	119, 496, 1096
BstSLI	GKGCMC	2 cut(s)	454, 1097
BstV2I	GAAGAC	1 cut(s)	1340
BstX2I	RGATCY	4 cut(s)	115, 913, 1218, 1327
BstYI	RGATCY	4 cut(s)	115, 913, 1218, 1327
BsuI	GTATCC	1 cut(s)	394
BsuRI	GGCC	6 cut(s)	261, 294, 452, 501, 807, 1095
BtsCI	GGATG	5 cut(s)	496, 691, 868, 939, 1141
BtsIMutI	CAGTG	4 cut(s)	535, 693, 1005, 1050
BveI	ACCTGC	1 cut(s)	1195
Cac8I	GCNNGC	3 cut(s)	450, 1093, 1255
CaiI	CAGNNNCTG	1 cut(s)	197
Cfr13I	GGNCC	5 cut(s)	450, 451, 866, 1093, 1094
Csp6I	GTAC	3 cut(s)	330, 557, 1470
CviAII	CATG	5 cut(s)	209, 263, 961, 1027, 1414
CviQI	GTAC	3 cut(s)	330, 557, 1470
DdeI	CTNAG	3 cut(s)	308, 639, 657
DraI	TTTAAA	1 cut(s)	819
EaeI	YGGCCR	2 cut(s)	259, 499
Eam1104I	CTCTTC	4 cut(s)	120, 227, 408, 1470
EarI	CTCTTC	4 cut(s)	120, 227, 408, 1470
Eco24I	GRGCYC	2 cut(s)	454, 1097
Eco32I	GATATC	1 cut(s)	7
Eco47I	GGWCC	1 cut(s)	866
Eco57I	CTGAAG	1 cut(s)	219
EcoO109I	RGGNCCY	1 cut(s)	1094
EcoRII	CCWGG	1 cut(s)	119
EcoRV	GATATC	1 cut(s)	7
EcoT38I	GRGCYC	2 cut(s)	454, 1097
FaeI	CATG	5 cut(s)	212, 266, 964, 1030, 1417
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FaqI	GGGAC	1 cut(s)	852
FatI	CATG	5 cut(s)	208, 262, 960, 1026, 1413
FauI	CCCGC	2 cut(s)	441, 1084
FbaI	TGATCA	3 cut(s)	145, 525, 1507
FokI	GGATG	5 cut(s)	503, 698, 875, 946, 1148
FriOI	GRGCYC	2 cut(s)	454, 1097
GsaI	CCCAGC	1 cut(s)	1544
HaeIII	GGCC	6 cut(s)	261, 294, 452, 501, 807, 1095
HapII	CCGG	3 cut(s)	83, 498, 1098
Hin1II	CATG	5 cut(s)	212, 266, 964, 1030, 1417
HinfI	GANTC	7 cut(s)	38, 130, 890, 951, 989, 1013, 1399
HpaII	CCGG	3 cut(s)	83, 498, 1098
HphI	GGTGA	2 cut(s)	1093, 1112
Hpy166II	GTNNAC	4 cut(s)	282, 623, 700, 1461
Hpy188I	TCNGA	5 cut(s)	23, 43, 145, 576, 1332
Hpy188III	TCNNGA	5 cut(s)	474, 931, 1246, 1372, 1426
Hpy8I	GTNNAC	4 cut(s)	282, 623, 700, 1461
HpyAV	CCTTC	7 cut(s)	175, 418, 1001, 1042, 1449, 1469, 1524
HpyCH4III	ACNGT	3 cut(s)	436, 697, 970
HpyCH4IV	ACGT	2 cut(s)	627, 1087
HpyF10VI	GCNNNNNNNGC	2 cut(s)	542, 793
HpyF3I	CTNAG	3 cut(s)	308, 639, 657
HpySE526I	ACGT	2 cut(s)	627, 1087
Hsp92II	CATG	5 cut(s)	212, 266, 964, 1030, 1417
Ksp22I	TGATCA	3 cut(s)	145, 525, 1507
LweI	GCATC	6 cut(s)	259, 481, 545, 767, 924, 1431
MaeII	ACGT	2 cut(s)	627, 1087
MaeIII	GTNAC	3 cut(s)	27, 1192, 1409
MflI	RGATCY	4 cut(s)	115, 913, 1218, 1327
MhlI	GDGCHC	2 cut(s)	454, 1097
MlsI	TGGCCA	1 cut(s)	261
MluCI	AATT	8 cut(s)	221, 297, 564, 584, 726, 788, 1199, 1519
MluNI	TGGCCA	1 cut(s)	261
MmeI	TCCRAC	1 cut(s)	1474
MnlI	CCTC	9 cut(s)	4, 7, 103, 228, 284, 403, 597, 915, 1126
Mox20I	TGGCCA	1 cut(s)	261
MroXI	GAANNNNTTC	2 cut(s)	129, 627
MscI	TGGCCA	1 cut(s)	261
MseI	TTAA	3 cut(s)	63, 753, 818
MslI	CAYNNNNRTG	2 cut(s)	243, 267
Msp20I	TGGCCA	1 cut(s)	261
MspI	CCGG	3 cut(s)	83, 498, 1098
MspR9I	CCNGG	3 cut(s)	121, 498, 1098
MvaI	CCWGG	1 cut(s)	121
MwoI	GCNNNNNNNGC	2 cut(s)	542, 793
NciI	CCSGG	2 cut(s)	498, 1098
NlaIII	CATG	5 cut(s)	212, 266, 964, 1030, 1417
NlaIV	GGNNCC	8 cut(s)	452, 744, 868, 1041, 1095, 1096, 1329, 1381
NspV	TTCGAA	1 cut(s)	879
PasI	CCCWGGG	1 cut(s)	120
PdmI	GAANNNNTTC	2 cut(s)	129, 627
PfeI	GAWTC	7 cut(s)	38, 130, 890, 951, 989, 1013, 1399
Pfl23II	CGTACG	1 cut(s)	329
PflMI	CCANNNNNTGG	1 cut(s)	1451
Ple19I	CGATCG	1 cut(s)	26
Psp6I	CCWGG	1 cut(s)	119
PspFI	CCCAGC	1 cut(s)	1540
PspGI	CCWGG	1 cut(s)	119
PspLI	CGTACG	1 cut(s)	329
PspN4I	GGNNCC	8 cut(s)	452, 744, 868, 1041, 1095, 1096, 1329, 1381
PspOMI	GGGCCC	2 cut(s)	450, 1093
PspPI	GGNCC	5 cut(s)	450, 451, 866, 1093, 1094
PstNI	CAGNNNCTG	1 cut(s)	197
PsuI	RGATCY	4 cut(s)	115, 913, 1218, 1327
PvuI	CGATCG	1 cut(s)	26
RsaI	GTAC	3 cut(s)	331, 558, 1471
RsaNI	GTAC	3 cut(s)	330, 557, 1470
RseI	CAYNNNNRTG	2 cut(s)	243, 267
SaqAI	TTAA	3 cut(s)	63, 753, 818
Sau96I	GGNCC	5 cut(s)	450, 451, 866, 1093, 1094
ScaI	AGTACT	1 cut(s)	558
ScrFI	CCNGG	3 cut(s)	121, 498, 1098
SduI	GDGCHC	2 cut(s)	454, 1097
SfaNI	GCATC	6 cut(s)	259, 481, 545, 767, 924, 1431
SfuI	TTCGAA	1 cut(s)	879
SinI	GGWCC	1 cut(s)	866
SmiMI	CAYNNNNRTG	2 cut(s)	243, 267
SmlI	CTYRAG	1 cut(s)	540
SmoI	CTYRAG	1 cut(s)	540
Sse9I	AATT	8 cut(s)	221, 297, 564, 584, 726, 788, 1199, 1519
SsiI	CCGC	4 cut(s)	312, 448, 1091, 1525
StyD4I	CCNGG	3 cut(s)	119, 496, 1096
TaaI	ACNGT	3 cut(s)	436, 697, 970
TaiI	ACGT	2 cut(s)	630, 1090
TaqI	TCGA	4 cut(s)	9, 879, 1166, 1425
TasI	AATT	8 cut(s)	221, 297, 564, 584, 726, 788, 1199, 1519
TatI	WGTACW	1 cut(s)	556
TfiI	GAWTC	7 cut(s)	38, 130, 890, 951, 989, 1013, 1399
Tru1I	TTAA	3 cut(s)	63, 753, 818
Tru9I	TTAA	3 cut(s)	63, 753, 818
TscAI	CASTG	4 cut(s)	535, 700, 1005, 1050
TspDTI	ATGAA	6 cut(s)	122, 360, 738, 915, 1152, 1364
TspRI	CASTG	4 cut(s)	535, 700, 1005, 1050
Van91I	CCANNNNNTGG	1 cut(s)	1451
VpaK11BI	GGWCC	1 cut(s)	866
XapI	RAATTY	3 cut(s)	297, 584, 1199
XcmI	CCANNNNNNNNNTGG	1 cut(s)	269
XmnI	GAANNNNTTC	2 cut(s)	129, 627
ZrmI	AGTACT	1 cut(s)	558

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.