Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0004512

Physical Location & Seq

Genomic Coordinates Reverse (-)

9682055 .. 9689456

Gene Structure 7402 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g17000.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGGATATTGAGGAGGAAATCCGGTCGCTGCAGCTGGATTCAGCAGAAGATAATGGGGATGTAAATCCAGAAGCTGCAAATCTGGATGTCGAGAAGTCGGATGATAAGATGGAGGAAGGTGATGATAAGATGGAGGAAGGTGATGGTAAGATGGAGGAAGATTCTGTTGACCCCGTGGCACATGAGCCAAAAGTAAAGGAGAAGGAAGAACAACATGACGAGGATGAAATAAATAAGAAGAGGCACTTGAATGTTGTTTTTATTGGCCATGTGGACGCTGGAAAGTCAACTACGGGAGGTCAAATCCTTTTCCTTAGTGGTCAGGTTGATGATCGTACAATTCAAAAATATGAAAAAGAAGCGAAAGACAAAAGTAGGGAGAGCTGGTATATGGCGTATATCATGGATACCAATGAAGAAGAGAGGCTTAAGGGTAAAACTGTTGAAGTTGGACGAGCACATTTTGAAACCGAGACAACTCGATTTACGATTTTGGATGCACCAGGTCACAAAAGTTATGTACCAAATATGATTAGTGGTGCATCTCAGGCTGATATAGGAGTACTGGTAATATCTGCTCGGAAAGGAGAATTTGAAACAGGGTATGAGAGAGGTGGACAGACCCGTGAACATGTTCAGCTTGCAAAAACATTGGGAGTGTCTAAACTGCTTGTTGTTGTCAATAAAATGGACGATCCTACTGTGAACTGGTCAAAAGAAAGGTATGATGAAATTGAGTCAAAGATGGTACCATTCCTTAAGACATCAGGCTACAACGTGAAGAAAGATGTGCAGTTCCTACCAATATCAGGTCTTATGGGTACAAACATGAAAACAAGATTGAATAAAGAAATTTGTTCATGGTGGGATGGCCCCTGCCTTTTTGAAGCCCTTGATGCTATTGAAGTTCCTCTACGGGACCCGAAAGGTCCATTCAGGTTGCCCATCATAGATAAATTCAAGGACATGGGAACTACTGTTATGGGGAAAGTGGAATCCGGTTCGGTGCGTGAAGGCGATTCACTGGTTGTCATGCCAAATAAGGCTCATGTGAAAGTGCTTGCTATATTCATTGATGAAGATAGGGTCAAATATGCTGGACCTGGTGAAAATCTTCGGGTTAGGTTATCAGGTATCGATGATGAGGACATACTGGCAGGTTTTGTCTTGTCAAGTGTGGCTAATCCAATACCTGCTGTTACTGAGTTTTATGCGCAGTTGCAGATCCTTGAGTTGCTGGAAAATGCAATCTTTACACCTGGATACAAAGCTGTCCTGCATATTCATGCTGTTGTTGAGGAATGTGAGATTGTTGATCTGATTGAGGAAATTGATATGAAGACTAAGAAACCTAAGAAGAAGAAGCCTCTTTTTGTAAAGAATGGTGCTGTAATTAAATGCCGGATTCAGGTGATTAACATGATATGCATGGAGAAGTTTGCCGATTTTCCCCAACTTGGAAGGTTTACTCTTCGTACTGAAGGTAAAACTATTGCTGTGGGAAAAGTAATCGATGCTCCATCGAAGAAAAGTAAACAATCTTCGACAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0002184 GO:0003674 GO:0003676 GO:0003723 GO:0003747 GO:0003824 GO:0003924 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006415 GO:0006464 GO:0006479 GO:0006518 GO:0006807 GO:0008079 GO:0008135 GO:0008150 GO:0008152 GO:0008213 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0018444 GO:0019538 GO:0022411 GO:0032259 GO:0032984 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0043043 GO:0043170 GO:0043412 GO:0043414 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0071840 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

518

Amino Acids

57.91

Weight (kDa)

5.16

Isoelectric Point (pI)

41.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	80 - 284	2.1e-48	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	324 - 390	9.4e-10	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	397 - 504	5e-24	Elongation factor Tu C-terminal domain
GTP-eEF1A_C	PF22594	402 - 503	6.6e-29	GTP-eEF1A C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18070 AT1G18070 AT1G18070
fragaria_vesca	FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000 FvH4_5g17000
malus_domestica	MD06G1224200.v1.1 MD14G1233200.v1.1
prunus_persica	Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1 Prupe.5G229200_v2.0.a1
pyrus_communis	pycom06g19950
rosa_chinensis	RchiOBHm_Chr7g0178871
rosa_laevigata	RLG00000005398
rosa_multiflora	Rmu_sc0000888.1_g000002
rosa_roxburghii	Rroxscaffold_3G00274490
rosa_rugosa	Rorug06G0420300 Rorug06G0420300 Rorug06G0420400 Rorug06G0420500 Rorug06G0420500 Rorug06G0420500
rosa_samantha	Rh7AG019800 Rh7BG019300 Rh7CG019900 Rh7DG019800
rosa_wichuraiana	Rw7G001600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1215
Acc36I	ACCTGC	2 cut(s)	1148, 1201
Acc65I	GGTACC	1 cut(s)	748
AccB1I	GGYRCC	1 cut(s)	748
AclWI	GGATC	2 cut(s)	689, 1219
AcoI	YGGCCR	1 cut(s)	265
AcsI	RAATTY	3 cut(s)	590, 852, 956
AcuI	CTGAAG	1 cut(s)	1500
AfaI	GTAC	6 cut(s)	337, 522, 564, 750, 823, 1477
AfiI	CCNNNNNNNGG	3 cut(s)	809, 1408, 1457
AflII	CTTAAG	2 cut(s)	428, 758
AflIII	ACRYGT	1 cut(s)	631
AgsI	TTSAA	9 cut(s)	250, 344, 446, 467, 596, 844, 887, 905, 961
AjnI	CCWGG	3 cut(s)	502, 1102, 1258
AluBI	AGCT	5 cut(s)	34, 74, 384, 640, 1271
AluI	AGCT	5 cut(s)	34, 74, 384, 640, 1271
Alw21I	GWGCWC	1 cut(s)	460
Alw26I	GTCTC	1 cut(s)	467
AlwI	GGATC	2 cut(s)	689, 1219
AlwNI	CAGNNNCTG	1 cut(s)	74
AoxI	GGCC	2 cut(s)	265, 871
ApeKI	GCWGC	3 cut(s)	28, 31, 74
ApoI	RAATTY	3 cut(s)	590, 852, 956
Asp700I	GAANNNNTTC	2 cut(s)	633, 1113
Asp718I	GGTACC	1 cut(s)	748
AspLEI	GCGC	1 cut(s)	1216
AspS9I	GGNCC	4 cut(s)	872, 919, 929, 1100
AsuHPI	GGTGA	4 cut(s)	131, 152, 1118, 1423
AvaII	GGWCC	3 cut(s)	919, 929, 1100
BalI	TGGCCA	1 cut(s)	267
BanI	GGYRCC	1 cut(s)	748
BarI	GAAGNNNNNNTAC	3 cut(s)	897, 929, 1525
BbsI	GAAGAC	1 cut(s)	1346
Bbv12I	GWGCWC	1 cut(s)	460
BbvI	GCAGC	3 cut(s)	15, 43, 61
BccI	CCATC	8 cut(s)	103, 124, 137, 145, 739, 863, 953, 1528
BciT130I	CCWGG	3 cut(s)	504, 1104, 1260
BciVI	GTATCC	2 cut(s)	400, 1256
BcoDI	GTCTC	1 cut(s)	467
BfmI	CTRYAG	1 cut(s)	29
BfrI	CTTAAG	2 cut(s)	428, 758
BfuAI	ACCTGC	2 cut(s)	1148, 1201
BfuI	GTATCC	2 cut(s)	400, 1256
BisI	GCNGC	3 cut(s)	29, 32, 75
BlsI	GCNGC	3 cut(s)	30, 33, 76
BmcAI	AGTACT	1 cut(s)	564
Bme1390I	CCNGG	3 cut(s)	504, 1104, 1260
Bme18I	GGWCC	3 cut(s)	919, 929, 1100
BmgT120I	GGNCC	4 cut(s)	872, 919, 929, 1100
BmiI	GGNNCC	4 cut(s)	750, 874, 920, 921
BmrFI	CCNGG	3 cut(s)	504, 1104, 1260
BmsI	GCATC	4 cut(s)	487, 551, 886, 1504
BpiI	GAAGAC	1 cut(s)	1346
BpuEI	CTTGAG	1 cut(s)	1250
Bsa29I	ATCGAT	2 cut(s)	1137, 1512
BsaBI	GATNNNNATC	1 cut(s)	63
BsaJI	CCNNGG	1 cut(s)	174
BsaWI	WCCGGW	2 cut(s)	21, 998
BsaXI	ACNNNNNCTCC	4 cut(s)	371, 401, 552, 582
Bsc4I	CCNNNNNNNGG	3 cut(s)	809, 1408, 1457
Bse1I	ACTGG	4 cut(s)	570, 713, 1029, 1158
Bse8I	GATNNNNATC	1 cut(s)	63
BseBI	CCWGG	3 cut(s)	504, 1104, 1260
BseCI	ATCGAT	2 cut(s)	1137, 1512
BseDI	CCNNGG	1 cut(s)	174
BseGI	GGATG	6 cut(s)	64, 91, 106, 229, 502, 874
BseJI	GATNNNNATC	1 cut(s)	63
BseLI	CCNNNNNNNGG	3 cut(s)	809, 1408, 1457
BseMII	CTCAG	2 cut(s)	560, 1194
BseNI	ACTGG	4 cut(s)	570, 713, 1029, 1158
BseRI	GAGGAG	1 cut(s)	26
BseXI	GCAGC	3 cut(s)	15, 43, 61
BsgI	GTGCAG	1 cut(s)	812
Bsh1285I	CGRYCG	1 cut(s)	26
BshFI	GGCC	2 cut(s)	267, 873
BshNI	GGYRCC	1 cut(s)	748
BshVI	ATCGAT	2 cut(s)	1137, 1512
BsiEI	CGRYCG	1 cut(s)	26
BsiHKAI	GWGCWC	1 cut(s)	460
BsiSI	CCGG	3 cut(s)	22, 999, 1402
BslFI	GGGAC	1 cut(s)	932
BslI	CCNNNNNNNGG	3 cut(s)	809, 1408, 1457
BsmAI	GTCTC	1 cut(s)	467
BsmFI	GGGAC	1 cut(s)	932
BsnI	GGCC	2 cut(s)	267, 873
Bsp1286I	GDGCHC	1 cut(s)	460
Bsp143I	GATC	4 cut(s)	331, 694, 1224, 1315
BspANI	GGCC	2 cut(s)	267, 873
BspCNI	CTCAG	2 cut(s)	559, 1195
BspDI	ATCGAT	2 cut(s)	1137, 1512
BspLI	GGNNCC	4 cut(s)	750, 874, 920, 921
BspMAI	CTGCAG	1 cut(s)	33
BspMI	ACCTGC	2 cut(s)	1148, 1201
BspPI	GGATC	2 cut(s)	689, 1219
BspT107I	GGYRCC	1 cut(s)	748
BspTI	CTTAAG	2 cut(s)	428, 758
BsrI	ACTGG	4 cut(s)	570, 713, 1029, 1158
BssECI	CCNNGG	1 cut(s)	174
BssMI	GATC	4 cut(s)	331, 694, 1224, 1315
Bst2UI	CCWGG	3 cut(s)	504, 1104, 1260
Bst4CI	ACNGT	3 cut(s)	442, 703, 979
Bst6I	CTCTTC	3 cut(s)	233, 414, 1476
BstAFI	CTTAAG	2 cut(s)	428, 758
BstC8I	GCNNGC	2 cut(s)	642, 1062
BstDEI	CTNAG	5 cut(s)	314, 546, 1203, 1344, 1353
BstDSI	CCRYGG	1 cut(s)	174
BstF5I	GGATG	6 cut(s)	64, 91, 106, 229, 502, 874
BstHHI	GCGC	1 cut(s)	1216
BstKTI	GATC	4 cut(s)	334, 697, 1227, 1318
BstMAI	GTCTC	1 cut(s)	467
BstMBI	GATC	4 cut(s)	331, 694, 1224, 1315
BstMCI	CGRYCG	1 cut(s)	26
BstMWI	GCNNNNNNNGC	2 cut(s)	548, 896
BstNI	CCWGG	3 cut(s)	504, 1104, 1260
BstNSI	RCATGY	1 cut(s)	635
BstSCI	CCNGG	3 cut(s)	502, 1102, 1258
BstSFI	CTRYAG	1 cut(s)	29
BstV1I	GCAGC	3 cut(s)	15, 43, 61
BstV2I	GAAGAC	1 cut(s)	1346
BstX2I	RGATCY	1 cut(s)	1224
BstYI	RGATCY	1 cut(s)	1224
Bsu15I	ATCGAT	2 cut(s)	1137, 1512
BsuI	GTATCC	2 cut(s)	400, 1256
BsuRI	GGCC	2 cut(s)	267, 873
BsuTUI	ATCGAT	2 cut(s)	1137, 1512
BtgI	CCRYGG	1 cut(s)	174
BtsCI	GGATG	6 cut(s)	64, 91, 106, 229, 502, 874
BtsIMutI	CAGTG	1 cut(s)	1022
BveI	ACCTGC	2 cut(s)	1148, 1201
Cac8I	GCNNGC	2 cut(s)	642, 1062
CaiI	CAGNNNCTG	1 cut(s)	74
CfoI	GCGC	1 cut(s)	1216
Cfr13I	GGNCC	4 cut(s)	872, 919, 929, 1100
ClaI	ATCGAT	2 cut(s)	1137, 1512
CseI	GACGC	1 cut(s)	284
CsiI	ACCWGGT	2 cut(s)	502, 1102
Csp6I	GTAC	6 cut(s)	336, 521, 563, 749, 822, 1476
CviQI	GTAC	6 cut(s)	336, 521, 563, 749, 822, 1476
DdeI	CTNAG	5 cut(s)	314, 546, 1203, 1344, 1353
DpnI	GATC	4 cut(s)	333, 696, 1226, 1317
DpnII	GATC	4 cut(s)	331, 694, 1224, 1315
EaeI	YGGCCR	1 cut(s)	265
Eam1104I	CTCTTC	3 cut(s)	233, 414, 1476
EarI	CTCTTC	3 cut(s)	233, 414, 1476
Eco47I	GGWCC	3 cut(s)	919, 929, 1100
Eco57I	CTGAAG	1 cut(s)	1500
EcoO109I	RGGNCCY	1 cut(s)	919
EcoRII	CCWGG	3 cut(s)	502, 1102, 1258
EcoT22I	ATGCAT	1 cut(s)	1430
FalI	AAGNNNNNCTT	4 cut(s)	230, 262, 411, 443
FaqI	GGGAC	1 cut(s)	932
Fnu4HI	GCNGC	3 cut(s)	29, 32, 75
FokI	GGATG	6 cut(s)	71, 98, 113, 236, 509, 881
Fsp4HI	GCNGC	3 cut(s)	29, 32, 75
FspI	TGCGCA	1 cut(s)	1215
GlaI	GCGC	1 cut(s)	1215
GluI	GCNGC	3 cut(s)	29, 32, 75
HaeIII	GGCC	2 cut(s)	267, 873
HapII	CCGG	3 cut(s)	22, 999, 1402
HgaI	GACGC	1 cut(s)	284
HhaI	GCGC	1 cut(s)	1216
Hin6I	GCGC	1 cut(s)	1214
HinP1I	GCGC	1 cut(s)	1214
HincII	GTYRAC	2 cut(s)	169, 288
HindII	GTYRAC	2 cut(s)	169, 288
HinfI	GANTC	6 cut(s)	38, 161, 737, 995, 1019, 1405
HpaII	CCGG	3 cut(s)	22, 999, 1402
HphI	GGTGA	4 cut(s)	131, 152, 1118, 1423
Hpy166II	GTNNAC	8 cut(s)	169, 274, 288, 617, 629, 706, 1467, 1535
Hpy188I	TCNGA	3 cut(s)	100, 582, 1320
Hpy188III	TCNNGA	3 cut(s)	68, 83, 91
Hpy8I	GTNNAC	8 cut(s)	169, 274, 288, 617, 629, 706, 1467, 1535
HpyAV	CCTTC	6 cut(s)	110, 131, 196, 1007, 1455, 1475
HpyCH4III	ACNGT	3 cut(s)	442, 703, 979
HpyCH4IV	ACGT	1 cut(s)	777
HpyF10VI	GCNNNNNNNGC	2 cut(s)	548, 896
HpyF3I	CTNAG	5 cut(s)	314, 546, 1203, 1344, 1353
HpySE526I	ACGT	1 cut(s)	777
HspAI	GCGC	1 cut(s)	1214
KflI	GGGWCCC	1 cut(s)	919
KpnI	GGTACC	1 cut(s)	752
Kzo9I	GATC	4 cut(s)	331, 694, 1224, 1315
LmnI	GCTCC	1 cut(s)	1522
Lsp1109I	GCAGC	3 cut(s)	15, 43, 61
LweI	GCATC	4 cut(s)	487, 551, 886, 1504
MabI	ACCWGGT	2 cut(s)	502, 1102
MaeII	ACGT	1 cut(s)	777
MaeIII	GTNAC	2 cut(s)	506, 1198
MalI	GATC	4 cut(s)	333, 696, 1226, 1317
MboI	GATC	4 cut(s)	331, 694, 1224, 1315
MflI	RGATCY	1 cut(s)	1224
MhlI	GDGCHC	1 cut(s)	460
MlsI	TGGCCA	1 cut(s)	267
MluCI	AATT	7 cut(s)	339, 590, 732, 852, 956, 1329, 1392
MluNI	TGGCCA	1 cut(s)	267
MlyI	GAGTC	1 cut(s)	746
MmeI	TCCRAC	2 cut(s)	78, 430
Mox20I	TGGCCA	1 cut(s)	267
Mph1103I	ATGCAT	1 cut(s)	1430
MroXI	GAANNNNTTC	2 cut(s)	633, 1113
MscI	TGGCCA	1 cut(s)	267
MseI	TTAA	4 cut(s)	429, 759, 1395, 1416
MslI	CAYNNNNRTG	2 cut(s)	249, 1284
Msp20I	TGGCCA	1 cut(s)	267
MspA1I	CMGCKG	1 cut(s)	34
MspCI	CTTAAG	2 cut(s)	428, 758
MspI	CCGG	3 cut(s)	22, 999, 1402
MspR9I	CCNGG	3 cut(s)	504, 1104, 1260
MvaI	CCWGG	3 cut(s)	504, 1104, 1260
MwoI	GCNNNNNNNGC	2 cut(s)	548, 896
NdeII	GATC	4 cut(s)	331, 694, 1224, 1315
NlaIV	GGNNCC	4 cut(s)	750, 874, 920, 921
NmuCI	GTSAC	1 cut(s)	506
NsbI	TGCGCA	1 cut(s)	1215
NsiI	ATGCAT	1 cut(s)	1430
NspI	RCATGY	1 cut(s)	635
PciI	ACATGT	1 cut(s)	631
PdmI	GAANNNNTTC	2 cut(s)	633, 1113
PfeI	GAWTC	5 cut(s)	38, 161, 995, 1019, 1405
PkrI	GCNGC	3 cut(s)	30, 33, 76
PleI	GAGTC	1 cut(s)	745
PpsI	GAGTC	1 cut(s)	745
PpuMI	RGGWCCY	1 cut(s)	919
PscI	ACATGT	1 cut(s)	631
Psp5II	RGGWCCY	1 cut(s)	919
Psp6I	CCWGG	3 cut(s)	502, 1102, 1258
PspGI	CCWGG	3 cut(s)	502, 1102, 1258
PspN4I	GGNNCC	4 cut(s)	750, 874, 920, 921
PspPI	GGNCC	4 cut(s)	872, 919, 929, 1100
PspPPI	RGGWCCY	1 cut(s)	919
PstI	CTGCAG	1 cut(s)	33
PstNI	CAGNNNCTG	1 cut(s)	74
PsuI	RGATCY	1 cut(s)	1224
PvuII	CAGCTG	1 cut(s)	34
RsaI	GTAC	6 cut(s)	337, 522, 564, 750, 823, 1477
RsaNI	GTAC	6 cut(s)	336, 521, 563, 749, 822, 1476
RseI	CAYNNNNRTG	2 cut(s)	249, 1284
SaqAI	TTAA	4 cut(s)	429, 759, 1395, 1416
SatI	GCNGC	3 cut(s)	29, 32, 75
Sau3AI	GATC	4 cut(s)	331, 694, 1224, 1315
Sau96I	GGNCC	4 cut(s)	872, 919, 929, 1100
ScaI	AGTACT	1 cut(s)	564
SchI	GAGTC	1 cut(s)	746
ScrFI	CCNGG	3 cut(s)	504, 1104, 1260
SduI	GDGCHC	1 cut(s)	460
SexAI	ACCWGGT	2 cut(s)	502, 1102
SfaNI	GCATC	4 cut(s)	487, 551, 886, 1504
SfcI	CTRYAG	1 cut(s)	29
SinI	GGWCC	3 cut(s)	919, 929, 1100
SmiMI	CAYNNNNRTG	2 cut(s)	249, 1284
SmlI	CTYRAG	3 cut(s)	428, 758, 1229
SmoI	CTYRAG	3 cut(s)	428, 758, 1229
Sse9I	AATT	7 cut(s)	339, 590, 732, 852, 956, 1329, 1392
StyD4I	CCNGG	3 cut(s)	502, 1102, 1258
TaaI	ACNGT	3 cut(s)	442, 703, 979
TaiI	ACGT	1 cut(s)	780
TaqI	TCGA	6 cut(s)	90, 481, 1137, 1512, 1523, 1544
TasI	AATT	7 cut(s)	339, 590, 732, 852, 956, 1329, 1392
TatI	WGTACW	1 cut(s)	562
TfiI	GAWTC	5 cut(s)	38, 161, 995, 1019, 1405
Tru1I	TTAA	4 cut(s)	429, 759, 1395, 1416
Tru9I	TTAA	4 cut(s)	429, 759, 1395, 1416
TscAI	CASTG	1 cut(s)	1029
TseFI	GTSAC	1 cut(s)	506
TseI	GCWGC	3 cut(s)	28, 31, 74
Tsp45I	GTSAC	1 cut(s)	506
TspRI	CASTG	1 cut(s)	1029
Vha464I	CTTAAG	2 cut(s)	428, 758
VpaK11BI	GGWCC	3 cut(s)	919, 929, 1100
XapI	RAATTY	3 cut(s)	590, 852, 956
XceI	RCATGY	1 cut(s)	635
XcmI	CCANNNNNNNNNTGG	1 cut(s)	275
XmnI	GAANNNNTTC	2 cut(s)	633, 1113
ZrmI	AGTACT	1 cut(s)	564
Zsp2I	ATGCAT	1 cut(s)	1430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.