AT1G19800

Belongs to the MlaE permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007305
Physical Location & Seq
Forward (+)
6846338 .. 6848128
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G19800.1

Sequence Viewer

Length: 1053 bp
ATGATGCAGACTTGTTGTATCCATCAATCGTTTTGTTTCCCTCATAGAGTCTTTCCACGGTTTGATGCTTCGATTGGTATTAAGCCCCCAAAGCTTTGTCAAGTTGGTTTCATTGGAAAGACTCAATCTTATGGGATTTCAAGTCCGATACGGCAAAGAAGATTATATGTGAATTTGAATGCTAATGATGGTCACCCATCCATGTCTATGTTGGAAGAAGAAACCTCTACTGAAAACAACGCACCCAGTCAAGAAGCCGAGCTTCCGTTCAGCAAATGGTCACCTTCTAAGTACATATGGAGAGGTTTATCAGTTCCTATTATAGCAGGACAAGTCGTTCTCCGGATTTTAAAGGGTAAGATTCACTGGAGAAACACTCTTCAACAGCTGGAGAGAACCGGACCGAAATCTCTAGGAGTTTGTCTTCTGACTTCTACATTTGTTGGTATGGCTTTCACAATCCAGTTCGTTAGAGAATTCACTAGACTAGGTCTAAACAGATCCATTGGAGGTGTCTTGGCTTTAGCCTTCTCTAGAGAGCTAAGTCCAGTCATCACATCGATTGTTGTTGCTGGACGAATGGGAAGTGCATTTGCAGCTGAACTAGGGACAATGCAAGTCTCAGAGCAAACTGATACACTCCGTGTTTTAGGAGCTGACCCAATTGATTATCTAATCACTCCAAGAGTCATCGCCTCGTGTTTGGCTCTACCGTTTCTGACACTCATGTGTTTCACTGTTGGTATGGCTTCAAGCGCTCTGCTCTCTGATGCAGTTTACGGGATCAGCATTAACATAATCATGGACTCGGCTCACCGAGCACTTAGACCATGGGACATTGTGAGTGCCATGATTAAATCTCAAGTCTTTGGAGCTATAATATCGGTAATTAGTTGTTCTTGGGGAGTAACCACTACTGGAGGTGCTAAAGGTGTTGGAGAATCTACAACTTCTGCTGTCGTCATGTCTCTTGTCGGAATCTTCATCGCGGACTTTGTGCTTTCTTCCTTCTTCTTTCAAGGTGCTGGAGATTCTTTGAAGAACTGTGTTTGA

Protein Analysis

350

Amino Acids

37.91

Weight (kDa)

9.01

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaE PF02405 127 - 336 4.9e-71 Permease MlaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 989
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 1 cut(s) 989
AclWI GGATC 2 cut(s) 495, 791
AcsI RAATTY 2 cut(s) 172, 476
AdeI CACNNNGTG 1 cut(s) 644
AfaI GTAC 1 cut(s) 293
AfeI AGCGCT 1 cut(s) 757
AgsI TTSAA 6 cut(s) 141, 178, 383, 753, 1019, 1039
AleI CACNNNNGTG 1 cut(s) 727
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 7 cut(s) 94, 262, 388, 541, 599, 656, 875
AluI AGCT 7 cut(s) 94, 262, 388, 541, 599, 656, 875
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 2 cut(s) 625, 972
AlwI GGATC 2 cut(s) 495, 791
Aor13HI TCCGGA 1 cut(s) 342
Aor51HI AGCGCT 1 cut(s) 757
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 2 cut(s) 172, 476
AspLEI GCGC 1 cut(s) 758
AspS9I GGNCC 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 185, 273, 806
AvaII GGWCC 1 cut(s) 401
BauI CACGAG 1 cut(s) 697
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 1 cut(s) 608
BccI CCATC 3 cut(s) 30, 182, 205
BceAI ACGGC 1 cut(s) 167
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 2 cut(s) 625, 972
BfaI CTAG 5 cut(s) 413, 483, 488, 534, 605
BfoI RGCGCY 1 cut(s) 759
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 1 cut(s) 597
BlsI GCNGC 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 401
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 2 cut(s) 55, 760
BmuI ACTGGG 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 361, 393
BpmI CTGGAG 4 cut(s) 388, 410, 939, 1047
BpuEI CTTGAG 1 cut(s) 846
Bsa29I ATCGAT 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 56, 830
BsaWI WCCGGW 2 cut(s) 342, 398
Bse1I ACTGG 5 cut(s) 246, 371, 463, 548, 922
BseAI TCCGGA 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 560
BseDI CCNNGG 2 cut(s) 56, 830
BseGI GGATG 1 cut(s) 197
BseMII CTCAG 1 cut(s) 636
BseNI ACTGG 5 cut(s) 246, 371, 463, 548, 922
BseXI GCAGC 1 cut(s) 608
Bsh1236I CGCG 1 cut(s) 989
BshVI ATCGAT 1 cut(s) 560
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 2 cut(s) 343, 399
BslFI GGGAC 2 cut(s) 622, 848
BsmAI GTCTC 2 cut(s) 625, 972
BsmFI GGGAC 2 cut(s) 622, 848
BsmI GAATGC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 823
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 2 cut(s) 500, 783
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 1 cut(s) 989
BspCNI CTCAG 1 cut(s) 635
BspDI ATCGAT 1 cut(s) 560
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 1 cut(s) 989
BspPI GGATC 2 cut(s) 495, 791
BsrI ACTGG 5 cut(s) 246, 371, 463, 548, 922
BssECI CCNNGG 2 cut(s) 56, 830
BssMI GATC 2 cut(s) 500, 783
BssSI CACGAG 1 cut(s) 697
BssT1I CCWWGG 1 cut(s) 830
Bst2BI CACGAG 1 cut(s) 697
Bst4CI ACNGT 4 cut(s) 60, 714, 739, 1046
Bst6I CTCTTC 1 cut(s) 384
BstDEI CTNAG 4 cut(s) 288, 542, 622, 824
BstDSI CCRYGG 2 cut(s) 56, 830
BstEII GGTNACC 2 cut(s) 191, 279
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 989
BstH2I RGCGCY 1 cut(s) 759
BstHHI GCGC 1 cut(s) 758
BstKTI GATC 2 cut(s) 503, 786
BstMAI GTCTC 2 cut(s) 625, 972
BstMBI GATC 2 cut(s) 500, 783
BstMWI GCNNNNNNNGC 4 cut(s) 91, 596, 755, 818
BstPI GGTNACC 2 cut(s) 191, 279
BstUI CGCG 1 cut(s) 989
BstV1I GCAGC 1 cut(s) 608
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
Bsu15I ATCGAT 1 cut(s) 560
BsuI GTATCC 1 cut(s) 29
BsuTUI ATCGAT 1 cut(s) 560
BtgI CCRYGG 2 cut(s) 56, 830
BtgZI GCGATG 2 cut(s) 676, 970
BtsCI GGATG 1 cut(s) 197
BtsIMutI CAGTG 2 cut(s) 364, 735
CfoI GCGC 1 cut(s) 758
Cfr13I GGNCC 1 cut(s) 401
ClaI ATCGAT 1 cut(s) 560
CpoI CGGWCCG 1 cut(s) 401
Csp6I GTAC 1 cut(s) 292
CspI CGGWCCG 1 cut(s) 401
CviAII CATG 6 cut(s) 202, 727, 802, 831, 850, 964
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 4 cut(s) 288, 542, 622, 824
DpnI GATC 2 cut(s) 502, 785
DpnII GATC 2 cut(s) 500, 783
DraI TTTAAA 1 cut(s) 351
DraIII CACNNNGTG 1 cut(s) 644
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco130I CCWWGG 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 401
Eco47III AGCGCT 1 cut(s) 757
Eco91I GGTNACC 2 cut(s) 191, 279
EcoO65I GGTNACC 2 cut(s) 191, 279
EcoRI GAATTC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 830
ErhI CCWWGG 1 cut(s) 830
FaeI CATG 6 cut(s) 205, 730, 805, 834, 853, 967
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 2 cut(s) 622, 848
FatI CATG 6 cut(s) 201, 726, 801, 830, 849, 963
FauNDI CATATG 1 cut(s) 296
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 597
FspBI CTAG 5 cut(s) 413, 483, 488, 534, 605
GlaI GCGC 1 cut(s) 757
GluI GCNGC 1 cut(s) 597
GsuI CTGGAG 4 cut(s) 388, 410, 939, 1047
HaeII RGCGCY 1 cut(s) 759
HapII CCGG 2 cut(s) 343, 399
HhaI GCGC 1 cut(s) 758
Hin1II CATG 6 cut(s) 205, 730, 805, 834, 853, 967
Hin6I GCGC 1 cut(s) 756
HinP1I GCGC 1 cut(s) 756
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 8 cut(s) 48, 121, 361, 687, 806, 941, 978, 1031
HpaII CCGG 2 cut(s) 343, 399
HphI GGTGA 3 cut(s) 185, 273, 806
Hpy166II GTNNAC 1 cut(s) 778
Hpy188I TCNGA 6 cut(s) 147, 429, 625, 720, 769, 977
Hpy188III TCNNGA 3 cut(s) 251, 343, 534
Hpy8I GTNNAC 1 cut(s) 778
HpyAV CCTTC 3 cut(s) 294, 538, 1018
HpyCH4III ACNGT 4 cut(s) 60, 714, 739, 1046
HpyCH4V TGCA 5 cut(s) 7, 590, 596, 616, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 596, 755, 818
HpyF3I CTNAG 4 cut(s) 288, 542, 622, 824
Hsp92II CATG 6 cut(s) 205, 730, 805, 834, 853, 967
HspAI GCGC 1 cut(s) 756
Kpn2I TCCGGA 1 cut(s) 342
Kzo9I GATC 2 cut(s) 500, 783
LmnI GCTCC 2 cut(s) 653, 872
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 2 cut(s) 55, 760
MaeI CTAG 5 cut(s) 413, 483, 488, 534, 605
MaeIII GTNAC 3 cut(s) 191, 279, 907
MalI GATC 2 cut(s) 502, 785
MboI GATC 2 cut(s) 500, 783
MboII GAAGA 9 cut(s) 171, 227, 230, 371, 416, 973, 996, 1003, 1051
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 823
MluCI AATT 4 cut(s) 172, 476, 663, 888
MlyI GAGTC 4 cut(s) 57, 115, 696, 800
MmeI TCCRAC 3 cut(s) 192, 916, 955
MnlI CCTC 6 cut(s) 51, 235, 296, 503, 706, 914
MroI TCCGGA 1 cut(s) 342
MseI TTAA 4 cut(s) 81, 350, 792, 855
MslI CAYNNNNRTG 3 cut(s) 206, 727, 800
MspA1I CMGCKG 2 cut(s) 388, 599
MspI CCGG 2 cut(s) 343, 399
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 184
MvnI CGCG 1 cut(s) 989
MwoI GCNNNNNNNGC 4 cut(s) 91, 596, 755, 818
NcoI CCATGG 1 cut(s) 830
NdeI CATATG 1 cut(s) 296
NdeII GATC 2 cut(s) 500, 783
NlaIII CATG 6 cut(s) 205, 730, 805, 834, 853, 967
NmeAIII GCCGAG 2 cut(s) 283, 788
NmuCI GTSAC 2 cut(s) 191, 279
OliI CACNNNNGTG 1 cut(s) 727
PctI GAATGC 1 cut(s) 184
PfeI GAWTC 4 cut(s) 361, 941, 978, 1031
PflFI GACNNNGTC 1 cut(s) 489
PkrI GCNGC 1 cut(s) 598
PleI GAGTC 4 cut(s) 56, 115, 695, 800
PpsI GAGTC 4 cut(s) 56, 115, 695, 800
PspEI GGTNACC 2 cut(s) 191, 279
PspPI GGNCC 1 cut(s) 401
PsuI RGATCY 1 cut(s) 500
PsyI GACNNNGTC 1 cut(s) 489
PvuII CAGCTG 2 cut(s) 388, 599
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 3 cut(s) 206, 727, 800
Rsr2I CGGWCCG 1 cut(s) 401
RsrII CGGWCCG 1 cut(s) 401
SaqAI TTAA 4 cut(s) 81, 350, 792, 855
SatI GCNGC 1 cut(s) 597
Sau3AI GATC 2 cut(s) 500, 783
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 4 cut(s) 57, 115, 696, 800
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 2 cut(s) 55, 760
SinI GGWCC 1 cut(s) 401
SmiMI CAYNNNNRTG 3 cut(s) 206, 727, 800
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 4 cut(s) 172, 476, 663, 888
SsiI CCGC 1 cut(s) 989
SspMI CTAG 5 cut(s) 413, 483, 488, 534, 605
StyI CCWWGG 1 cut(s) 830
TaaI ACNGT 4 cut(s) 60, 714, 739, 1046
TaqI TCGA 2 cut(s) 71, 560
TaqII GACCGA 1 cut(s) 418
TasI AATT 4 cut(s) 172, 476, 663, 888
TatI WGTACW 1 cut(s) 291
TfiI GAWTC 4 cut(s) 361, 941, 978, 1031
Tru1I TTAA 4 cut(s) 81, 350, 792, 855
Tru9I TTAA 4 cut(s) 81, 350, 792, 855
TscAI CASTG 2 cut(s) 371, 742
TseFI GTSAC 2 cut(s) 191, 279
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 2 cut(s) 191, 279
TspDTI ATGAA 2 cut(s) 100, 973
TspGWI ACGGA 2 cut(s) 255, 632
TspRI CASTG 2 cut(s) 371, 742
Tth111I GACNNNGTC 1 cut(s) 489
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 2 cut(s) 172, 476
XbaI TCTAGA 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XspI CTAG 5 cut(s) 413, 483, 488, 534, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.