MD05G1209200.v1.1

Belongs to the MlaE permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007305
Physical Location & Seq
Forward (+)
34088917 .. 34091448
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1209200.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGCAAACCGCTTCATATCTTCATCCGTTCTCTTATTTTGCTGACAGAAGAAGCCTTTTGAAACCAGATGGGTGGAGTAAATCACAAACCTCATATTCAAGTGATCTTGTCAGGAGAGTTCTGATTACTAGAAGACCTCAGACATGTAAGTTTCTCTTGCCTAAGCAACAAACCAGATTGTTTGCAATTCCCAACATAGATGATGGCCACCCTTCTGCCTCTGTGTTAGAAGATTCAAATACAAATCATGCACCCAATTCAGAAGCGAAGACGGTGTTGCACAAATGGTCACCTCCCACGTATATGTGGAGGGGGTTTTCAGCTCTTATCCTGGCAGGACAGGTTATTTTGAGAACTTTGAAGGGCAAAGTGCACTGGGGAAATACACTTCAGCAATTAAAGAGAGTTGGGCCAGGTTCAGTCGGGGTCTGTCTCTTGACTTCAACATTTGTTGGCATGGCCTTTACGATCCAATTCGTTAGGGAATTTACTAGATTAGGGTTAAGCAGAGCCGTTGGTGGGGTTTTAGCACTGGCTTTCTCAAGGGAGTTGAGTCCTGTAATTACATCAATCGTGGTTGCTGGGCGTATTGGAAGCTCATTTGCTGCAGAGTTGGGAACAATGCAGGTTTCTGAGCAAACCGACACTCTGAGAGTTCTTGGGGCTGACCCGGTTGATTATCTTGTGACTCCAAGGGTGATTGCTACATGTATTGCTCTTCCGTTTCTGACTCTAATGTGTTTCTCAGTAGGGATGGCATCCAGCGCCCTCCTTTCCGATGGCATTTATGGTGTTAGCATCAACATTATATTGGATTCAGCTTGCAGAGCTCTCAAACCATGGGATATAATTAGTGCAATGATCAAGTCACAGGTTTTTGGTGCGATCATATCGATTGTGAGTTGTGCTTGGGGAGTGACCACTATGGGAGGAGCCAAGGGTGTTGGAGAGTCAACAACTTCGGCTGTGGTTATATCTCTTGTTGGGATCTTTATCGCAGATTTTGTACTCTCGTGTTGTTTCTTCCAGGGAGTTGGAGATTCATTGAAGAGGCTCTAA

Protein Analysis

353

Amino Acids

37.95

Weight (kDa)

9.44

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaE PF02405 130 - 338 1e-68 Permease MlaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 616
AciI CCGC 1 cut(s) 9
AclWI GGATC 2 cut(s) 463, 995
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 1 cut(s) 1008
AfiI CCNNNNNNNGG 1 cut(s) 519
AflIII ACRYGT 2 cut(s) 143, 707
AgsI TTSAA 6 cut(s) 61, 99, 237, 361, 444, 1048
AjnI CCWGG 3 cut(s) 330, 412, 1026
AluBI AGCT 4 cut(s) 323, 597, 821, 830
AluI AGCT 4 cut(s) 323, 597, 821, 830
Alw21I GWGCWC 2 cut(s) 375, 832
Alw26I GTCTC 1 cut(s) 437
Alw44I GTGCAC 1 cut(s) 371
AlwI GGATC 2 cut(s) 463, 995
AoxI GGCC 3 cut(s) 205, 410, 459
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 1 cut(s) 485
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 410
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 2 cut(s) 282, 709
BaeGI GKGCMC 1 cut(s) 375
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 1 cut(s) 832
BauI CACGAG 1 cut(s) 1012
BbsI GAAGAC 2 cut(s) 139, 275
Bbv12I GWGCWC 2 cut(s) 375, 832
BbvI GCAGC 1 cut(s) 592
BccI CCATC 4 cut(s) 62, 197, 748, 773
BceAI ACGGC 1 cut(s) 497
BciT130I CCWGG 3 cut(s) 332, 414, 1028
BclI TGATCA 1 cut(s) 861
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 2 cut(s) 129, 492
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 616
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 4 cut(s) 332, 414, 671, 1028
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 1 cut(s) 934
BmrFI CCNGG 4 cut(s) 332, 414, 671, 1028
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 2 cut(s) 767, 807
BmuI ACTGGG 1 cut(s) 385
BpiI GAAGAC 2 cut(s) 139, 275
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 1 cut(s) 671
Bsa29I ATCGAT 1 cut(s) 893
BsaAI YACGTR 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 992
BsaJI CCNNGG 4 cut(s) 692, 839, 936, 1027
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 380, 537
Bse3DI GCAATG 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 992
BseBI CCWGG 3 cut(s) 332, 414, 1028
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 4 cut(s) 692, 839, 936, 1027
BseGI GGATG 3 cut(s) 22, 758, 759
BseJI GATNNNNATC 1 cut(s) 992
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 4 cut(s) 152, 624, 641, 759
BseNI ACTGG 2 cut(s) 380, 537
BseRI GAGGAG 1 cut(s) 945
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 581
BshFI GGCC 3 cut(s) 207, 412, 461
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 375, 832
BsiSI CCGG 1 cut(s) 671
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 1 cut(s) 437
BsnI GGCC 3 cut(s) 207, 412, 461
Bsp1286I GDGCHC 2 cut(s) 375, 832
Bsp143I GATC 5 cut(s) 103, 468, 861, 885, 987
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 1 cut(s) 9
BspANI GGCC 3 cut(s) 207, 412, 461
BspCNI CTCAG 4 cut(s) 151, 625, 642, 758
BspDI ATCGAT 1 cut(s) 893
BspLI GGNNCC 1 cut(s) 934
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 2 cut(s) 463, 995
BspQI GCTCTTC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 2 cut(s) 380, 537
BssECI CCNNGG 4 cut(s) 692, 839, 936, 1027
BssMI GATC 5 cut(s) 103, 468, 861, 885, 987
BssSI CACGAG 1 cut(s) 1012
BssT1I CCWWGG 3 cut(s) 692, 839, 936
Bst2BI CACGAG 1 cut(s) 1012
Bst2UI CCWGG 3 cut(s) 332, 414, 1028
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 2 cut(s) 723, 1043
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 823
BstDEI CTNAG 5 cut(s) 138, 162, 633, 650, 745
BstDSI CCRYGG 1 cut(s) 839
BstEII GGTNACC 1 cut(s) 288
BstF5I GGATG 3 cut(s) 22, 758, 759
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 5 cut(s) 106, 471, 864, 888, 990
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 5 cut(s) 103, 468, 861, 885, 987
BstMWI GCNNNNNNNGC 2 cut(s) 764, 827
BstNI CCWGG 3 cut(s) 332, 414, 1028
BstNSI RCATGY 2 cut(s) 147, 711
BstPI GGTNACC 1 cut(s) 288
BstSCI CCNGG 4 cut(s) 330, 412, 669, 1026
BstSFI CTRYAG 1 cut(s) 606
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 592
BstV2I GAAGAC 2 cut(s) 139, 275
BstX2I RGATCY 1 cut(s) 987
BstXI CCANNNNNNTGG 2 cut(s) 72, 1034
BstYI RGATCY 1 cut(s) 987
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 3 cut(s) 207, 412, 461
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 839
BtsCI GGATG 3 cut(s) 22, 758, 759
BtsIMutI CAGTG 2 cut(s) 373, 530
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 1 cut(s) 823
CfoI GCGC 1 cut(s) 767
Cfr13I GGNCC 1 cut(s) 410
ClaI ATCGAT 1 cut(s) 893
Csp6I GTAC 1 cut(s) 1007
CviAII CATG 5 cut(s) 144, 248, 457, 708, 840
CviQI GTAC 1 cut(s) 1007
DdeI CTNAG 5 cut(s) 138, 162, 633, 650, 745
DpnI GATC 5 cut(s) 105, 470, 863, 887, 989
DpnII GATC 5 cut(s) 103, 468, 861, 885, 987
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 723, 1043
EarI CTCTTC 2 cut(s) 723, 1043
Ecl136II GAGCTC 1 cut(s) 830
Eco130I CCWWGG 3 cut(s) 692, 839, 936
Eco24I GRGCYC 1 cut(s) 832
Eco53kI GAGCTC 1 cut(s) 830
Eco57I CTGAAG 1 cut(s) 374
Eco91I GGTNACC 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 830
EcoO65I GGTNACC 1 cut(s) 288
EcoRII CCWGG 3 cut(s) 330, 412, 1026
EcoT14I CCWWGG 3 cut(s) 692, 839, 936
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 3 cut(s) 692, 839, 936
FaeI CATG 5 cut(s) 147, 251, 460, 711, 843
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 5 cut(s) 143, 247, 456, 707, 839
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 3 cut(s) 9, 745, 766
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 129, 492
GlaI GCGC 1 cut(s) 766
GluI GCNGC 1 cut(s) 606
GsaI CCCAGC 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 3 cut(s) 207, 412, 461
HapII CCGG 1 cut(s) 671
HhaI GCGC 1 cut(s) 767
Hin1II CATG 5 cut(s) 147, 251, 460, 711, 843
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 954
HindII GTYRAC 1 cut(s) 954
HinfI GANTC 7 cut(s) 233, 553, 688, 730, 815, 950, 1040
HpaII CCGG 1 cut(s) 671
HphI GGTGA 2 cut(s) 282, 709
Hpy166II GTNNAC 2 cut(s) 373, 954
Hpy188I TCNGA 7 cut(s) 123, 141, 262, 634, 651, 729, 778
Hpy188III TCNNGA 2 cut(s) 112, 436
Hpy8I GTNNAC 2 cut(s) 373, 954
HpyAV CCTTC 2 cut(s) 222, 355
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 9 cut(s) 4, 185, 251, 280, 373, 608, 625, 825, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 764, 827
HpyF3I CTNAG 5 cut(s) 138, 162, 633, 650, 745
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 5 cut(s) 147, 251, 460, 711, 843
HspAI GCGC 1 cut(s) 765
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 5 cut(s) 103, 468, 861, 885, 987
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 1 cut(s) 932
Lsp1109I GCAGC 1 cut(s) 592
LweI GCATC 2 cut(s) 767, 807
MaeI CTAG 2 cut(s) 129, 492
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 4 cut(s) 288, 685, 867, 916
MalI GATC 5 cut(s) 105, 470, 863, 887, 989
MboI GATC 5 cut(s) 103, 468, 861, 885, 987
MboII GAAGA 7 cut(s) 11, 60, 144, 242, 280, 710, 1015
MflI RGATCY 1 cut(s) 987
MhlI GDGCHC 2 cut(s) 375, 832
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 7 cut(s) 186, 256, 395, 473, 485, 561, 849
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 4 cut(s) 562, 682, 724, 959
MmeI TCCRAC 2 cut(s) 925, 1015
MnlI CCTC 8 cut(s) 100, 147, 229, 303, 303, 779, 923, 1044
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 2 cut(s) 398, 503
MslI CAYNNNNRTG 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 1 cut(s) 671
MspR9I CCNGG 4 cut(s) 332, 414, 671, 1028
MvaI CCWGG 3 cut(s) 332, 414, 1028
MwoI GCNNNNNNNGC 2 cut(s) 764, 827
NciI CCSGG 1 cut(s) 671
NcoI CCATGG 1 cut(s) 839
NdeII GATC 5 cut(s) 103, 468, 861, 885, 987
NlaIII CATG 5 cut(s) 147, 251, 460, 711, 843
NlaIV GGNNCC 1 cut(s) 934
NmuCI GTSAC 4 cut(s) 288, 685, 867, 916
NspI RCATGY 2 cut(s) 147, 711
PciI ACATGT 2 cut(s) 143, 707
PciSI GCTCTTC 1 cut(s) 723
PfeI GAWTC 3 cut(s) 233, 815, 1040
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 4 cut(s) 561, 682, 724, 958
PpsI GAGTC 4 cut(s) 561, 682, 724, 958
Ppu21I YACGTR 1 cut(s) 300
PscI ACATGT 2 cut(s) 143, 707
Psp124BI GAGCTC 1 cut(s) 832
Psp6I CCWGG 3 cut(s) 330, 412, 1026
PspEI GGTNACC 1 cut(s) 288
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 3 cut(s) 330, 412, 1026
PspN4I GGNNCC 1 cut(s) 934
PspPI GGNCC 1 cut(s) 410
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 987
RsaI GTAC 1 cut(s) 1008
RsaNI GTAC 1 cut(s) 1007
RseI CAYNNNNRTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 832
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 2 cut(s) 398, 503
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 5 cut(s) 103, 468, 861, 885, 987
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 4 cut(s) 562, 682, 724, 959
ScrFI CCNGG 4 cut(s) 332, 414, 671, 1028
SduI GDGCHC 2 cut(s) 375, 832
SfaNI GCATC 2 cut(s) 767, 807
SfcI CTRYAG 1 cut(s) 606
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
Sse9I AATT 7 cut(s) 186, 256, 395, 473, 485, 561, 849
SsiI CCGC 1 cut(s) 9
SspMI CTAG 2 cut(s) 129, 492
SstI GAGCTC 1 cut(s) 832
StyD4I CCNGG 4 cut(s) 330, 412, 669, 1026
StyI CCWWGG 3 cut(s) 692, 839, 936
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 893
TasI AATT 7 cut(s) 186, 256, 395, 473, 485, 561, 849
TatI WGTACW 1 cut(s) 1006
TfiI GAWTC 3 cut(s) 233, 815, 1040
Tru1I TTAA 2 cut(s) 398, 503
Tru9I TTAA 2 cut(s) 398, 503
TscAI CASTG 2 cut(s) 380, 537
TseFI GTSAC 4 cut(s) 288, 685, 867, 916
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 4 cut(s) 288, 685, 867, 916
TspDTI ATGAA 2 cut(s) 11, 1032
TspGWI ACGGA 2 cut(s) 15, 711
TspRI CASTG 2 cut(s) 380, 537
VneI GTGCAC 1 cut(s) 371
XapI RAATTY 1 cut(s) 485
XceI RCATGY 2 cut(s) 147, 711
XspI CTAG 2 cut(s) 129, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.