pycom05g19530

Belongs to the MlaE permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007305
Physical Location & Seq
Forward (+)
22448730 .. 22449741
1012 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g19530.1

Sequence Viewer

Length: 906 bp
ATGCAAACCGCTTCATATCTTCATCCATTCTCTTATTTTTCTGACAGAAGAAACCATTTAAAACCAGATGGGTGGAGTAAATCACAAACCTCATATTCAAGTGATCTTGTTAGGAGAGTTCTGATTACTAGAAGACCTCAGACGTGTAAGTTTCTCTTGCCTAAGCAACAAACCAGATTGTATGCAATTCCCAACACGGATGATGGCCACCCTTCTGCCTCTGTGTTAGAAGATTCAAATACAAATCATGCACCCAATTCGGAAGCGAAGACAGTGTTGCACAAATGGTCACCTCCCACGTATATGTGGAGGGGGTTTTCAGCTCTTATCCTGGCAGGACAGGTTATTTTGAGAACTTTGAAGGGCAAGGTGCACTGGGGAAATACACTCCAGCAATTAAGGAGAGTTGGGCCAGGTTCAGTCGGGGTCTGTCTCTTGACTTCAACATTTGTTGGCATGGCCTTTACAATCCAATTCGTTAGGGAATTTACTAGATTAGGGTTAAGCAGAGCCGTTGGTGGGGTTTTAGCACTGGCTTTCTCAAGGGAGTTGAGTCCTGTAATTACATCAATCGTGGTTGCTGGGCGTATTGGAAGCTCATTTGCAGCAGAGTTGGGAACAATGCAGGTTTCTGAGCAAACCGACACTCTGAGAGTTCTTGGGGCAGACCCGGTTGATTATCTTGTGACTCCGAGGGTGATTGCTACATGTATTGCCCTTCCGTTTCTGACTCTAATGTGTTTCTCAGTAGGGATGGCATCCAGCGCCCTCCTTTCCGATGGCATTTATGGTGTTAGCATCAACATTATATTGGATTCAGCTTGCCGAGCTCTCAAGCCATGGGATATAATTAGTGCAATAGATCGATCAAGTCACAGGTTTTTGGTGCGATCATATCAATTGTGA

Protein Analysis

302

Amino Acids

33.15

Weight (kDa)

9.8

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaE PF02405 130 - 287 1.5e-48 Permease MlaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 616
AciI CCGC 1 cut(s) 9
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 485
AfiI CCNNNNNNNGG 1 cut(s) 519
AflIII ACRYGT 2 cut(s) 143, 707
AgsI TTSAA 4 cut(s) 99, 237, 361, 444
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 330, 412
AluBI AGCT 4 cut(s) 323, 597, 821, 830
AluI AGCT 4 cut(s) 323, 597, 821, 830
Alw21I GWGCWC 2 cut(s) 375, 832
Alw26I GTCTC 1 cut(s) 437
Alw44I GTGCAC 1 cut(s) 371
AoxI GGCC 3 cut(s) 205, 410, 459
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 1 cut(s) 485
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 410
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 2 cut(s) 282, 709
BaeGI GKGCMC 1 cut(s) 375
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 1 cut(s) 832
BbsI GAAGAC 2 cut(s) 139, 275
Bbv12I GWGCWC 2 cut(s) 375, 832
BbvI GCAGC 1 cut(s) 617
BccI CCATC 4 cut(s) 62, 197, 748, 773
BceAI ACGGC 1 cut(s) 497
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BciT130I CCWGG 2 cut(s) 332, 414
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 2 cut(s) 129, 492
BfoI RGCGCY 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 616
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 3 cut(s) 332, 414, 671
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 410
BmrFI CCNGG 3 cut(s) 332, 414, 671
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 2 cut(s) 767, 807
BmuI ACTGGG 1 cut(s) 385
BpiI GAAGAC 2 cut(s) 139, 275
BpmI CTGGAG 1 cut(s) 374
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 2 cut(s) 526, 818
BpuMI CCSGG 1 cut(s) 671
Bsa29I ATCGAT 1 cut(s) 865
BsaAI YACGTR 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 692, 839
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 380, 537
BseBI CCWGG 2 cut(s) 332, 414
BseCI ATCGAT 1 cut(s) 865
BseDI CCNNGG 2 cut(s) 692, 839
BseGI GGATG 4 cut(s) 22, 205, 758, 759
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMII CTCAG 4 cut(s) 152, 624, 641, 759
BseNI ACTGG 2 cut(s) 380, 537
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 617
BseYI CCCAGC 1 cut(s) 581
BshFI GGCC 3 cut(s) 207, 412, 461
BshVI ATCGAT 1 cut(s) 865
BsiHKAI GWGCWC 2 cut(s) 375, 832
BsiSI CCGG 1 cut(s) 671
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 1 cut(s) 437
BsnI GGCC 3 cut(s) 207, 412, 461
Bsp1286I GDGCHC 2 cut(s) 375, 832
Bsp143I GATC 4 cut(s) 103, 862, 866, 890
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 1 cut(s) 9
BspANI GGCC 3 cut(s) 207, 412, 461
BspCNI CTCAG 4 cut(s) 151, 625, 642, 758
BspDI ATCGAT 1 cut(s) 865
BspMI ACCTGC 1 cut(s) 616
BsrI ACTGG 2 cut(s) 380, 537
BssECI CCNNGG 2 cut(s) 692, 839
BssMI GATC 4 cut(s) 103, 862, 866, 890
BssT1I CCWWGG 1 cut(s) 839
Bst2UI CCWGG 2 cut(s) 332, 414
Bst4CI ACNGT 1 cut(s) 274
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 823
BstDEI CTNAG 5 cut(s) 138, 162, 633, 650, 745
BstDSI CCRYGG 1 cut(s) 839
BstEII GGTNACC 1 cut(s) 288
BstF5I GGATG 4 cut(s) 22, 205, 758, 759
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 4 cut(s) 106, 865, 869, 893
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 4 cut(s) 103, 862, 866, 890
BstMWI GCNNNNNNNGC 2 cut(s) 764, 827
BstNI CCWGG 2 cut(s) 332, 414
BstNSI RCATGY 1 cut(s) 711
BstPI GGTNACC 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 330, 412, 669
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 617
BstV2I GAAGAC 2 cut(s) 139, 275
BstXI CCANNNNNNTGG 1 cut(s) 72
Bsu15I ATCGAT 1 cut(s) 865
BsuRI GGCC 3 cut(s) 207, 412, 461
BsuTUI ATCGAT 1 cut(s) 865
BtgI CCRYGG 1 cut(s) 839
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 4 cut(s) 22, 205, 758, 759
BtsIMutI CAGTG 3 cut(s) 279, 373, 530
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 1 cut(s) 823
CfoI GCGC 1 cut(s) 767
Cfr13I GGNCC 1 cut(s) 410
ClaI ATCGAT 1 cut(s) 865
CviAII CATG 4 cut(s) 248, 457, 708, 840
DdeI CTNAG 5 cut(s) 138, 162, 633, 650, 745
DpnI GATC 4 cut(s) 105, 864, 868, 892
DpnII GATC 4 cut(s) 103, 862, 866, 890
DraI TTTAAA 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 205
Ecl136II GAGCTC 1 cut(s) 830
Eco130I CCWWGG 1 cut(s) 839
Eco24I GRGCYC 1 cut(s) 832
Eco53kI GAGCTC 1 cut(s) 830
Eco91I GGTNACC 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 830
EcoO65I GGTNACC 1 cut(s) 288
EcoRII CCWGG 2 cut(s) 330, 412
EcoT14I CCWWGG 1 cut(s) 839
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 1 cut(s) 839
FaeI CATG 4 cut(s) 251, 460, 711, 843
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 4 cut(s) 247, 456, 707, 839
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 4 cut(s) 9, 212, 745, 766
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 2 cut(s) 129, 492
GlaI GCGC 1 cut(s) 766
GluI GCNGC 1 cut(s) 606
GsaI CCCAGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 3 cut(s) 207, 412, 461
HapII CCGG 1 cut(s) 671
HhaI GCGC 1 cut(s) 767
Hin1II CATG 4 cut(s) 251, 460, 711, 843
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HinfI GANTC 5 cut(s) 233, 553, 688, 730, 815
HpaII CCGG 1 cut(s) 671
HphI GGTGA 2 cut(s) 282, 709
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 9 cut(s) 43, 123, 141, 262, 634, 651, 693, 729, 778
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 3 cut(s) 222, 355, 728
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 2 cut(s) 143, 299
HpyCH4V TGCA 8 cut(s) 4, 185, 251, 280, 373, 605, 625, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 764, 827
HpyF3I CTNAG 5 cut(s) 138, 162, 633, 650, 745
HpySE526I ACGT 2 cut(s) 143, 299
Hsp92II CATG 4 cut(s) 251, 460, 711, 843
HspAI GCGC 1 cut(s) 765
Kzo9I GATC 4 cut(s) 103, 862, 866, 890
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 2 cut(s) 767, 807
MaeI CTAG 2 cut(s) 129, 492
MaeII ACGT 2 cut(s) 143, 299
MaeIII GTNAC 3 cut(s) 288, 685, 872
MalI GATC 4 cut(s) 105, 864, 868, 892
MboI GATC 4 cut(s) 103, 862, 866, 890
MboII GAAGA 5 cut(s) 11, 60, 144, 242, 280
MfeI CAATTG 1 cut(s) 899
MhlI GDGCHC 2 cut(s) 375, 832
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 8 cut(s) 186, 256, 395, 473, 485, 561, 849, 899
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 3 cut(s) 562, 682, 724
MnlI CCTC 7 cut(s) 100, 147, 229, 303, 303, 687, 779
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 3 cut(s) 59, 398, 503
MslI CAYNNNNRTG 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 1 cut(s) 671
MspR9I CCNGG 3 cut(s) 332, 414, 671
MunI CAATTG 1 cut(s) 899
MvaI CCWGG 2 cut(s) 332, 414
MwoI GCNNNNNNNGC 2 cut(s) 764, 827
NciI CCSGG 1 cut(s) 671
NcoI CCATGG 1 cut(s) 839
NdeII GATC 4 cut(s) 103, 862, 866, 890
NlaIII CATG 4 cut(s) 251, 460, 711, 843
NmeAIII GCCGAG 1 cut(s) 851
NmuCI GTSAC 3 cut(s) 288, 685, 872
NspI RCATGY 1 cut(s) 711
PciI ACATGT 1 cut(s) 707
PfeI GAWTC 2 cut(s) 233, 815
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 3 cut(s) 561, 682, 724
PpsI GAGTC 3 cut(s) 561, 682, 724
Ppu21I YACGTR 1 cut(s) 300
PscI ACATGT 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 832
Psp6I CCWGG 2 cut(s) 330, 412
PspEI GGTNACC 1 cut(s) 288
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 2 cut(s) 330, 412
PspPI GGNCC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 832
SaqAI TTAA 3 cut(s) 59, 398, 503
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 4 cut(s) 103, 862, 866, 890
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 3 cut(s) 562, 682, 724
ScrFI CCNGG 3 cut(s) 332, 414, 671
SduI GDGCHC 2 cut(s) 375, 832
SfaNI GCATC 2 cut(s) 767, 807
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 2 cut(s) 541, 833
SmoI CTYRAG 2 cut(s) 541, 833
Sse9I AATT 8 cut(s) 186, 256, 395, 473, 485, 561, 849, 899
SsiI CCGC 1 cut(s) 9
SspMI CTAG 2 cut(s) 129, 492
SstI GAGCTC 1 cut(s) 832
StyD4I CCNGG 3 cut(s) 330, 412, 669
StyI CCWWGG 1 cut(s) 839
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 2 cut(s) 146, 302
TaqI TCGA 1 cut(s) 865
TasI AATT 8 cut(s) 186, 256, 395, 473, 485, 561, 849, 899
TfiI GAWTC 2 cut(s) 233, 815
Tru1I TTAA 3 cut(s) 59, 398, 503
Tru9I TTAA 3 cut(s) 59, 398, 503
TscAI CASTG 3 cut(s) 279, 380, 537
TseFI GTSAC 3 cut(s) 288, 685, 872
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 3 cut(s) 288, 685, 872
TspDTI ATGAA 1 cut(s) 11
TspGWI ACGGA 2 cut(s) 212, 711
TspRI CASTG 3 cut(s) 279, 380, 537
VneI GTGCAC 1 cut(s) 371
XapI RAATTY 1 cut(s) 485
XceI RCATGY 1 cut(s) 711
XspI CTAG 2 cut(s) 129, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.