MD10G1195600.v1.1

Belongs to the MlaE permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007305
Physical Location & Seq
Forward (+)
29400494 .. 29402909
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1195600.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGCAAACCGCTTCATATCTTCATCTGTTCTCTTATATTTCTGACAGAAGAAGCCTTTTAAAACCAGTAGGGTGGATTAAATCACAAACCCCATATTCAAATCATCTTGATAGGAGAGTTCCGATTACTGTAAGACCTCAGTCGTGTAAGTTTCTCTTGCTGAAGCAACAAACCAGATTGTTTGCAGTTCCCAACACAGATGATGGCCATCCTTCTGCCTCTGTGTTAGAAGATTCAAACACAAATCATGCACCCAGTCCGGAAGTGGAGACGGTGTTGCACAAATGGTCACCTCCCACGTATTTGTGGAGGGGGTTTTCAGCTCTTATCCTGACAGGACAGGTTATTTTGAGAACCTTGAAGGGCAAAGTGCACTGGGGAAATACACTTCAGCAATTAAAGAGAGTTGGACCTGGTTCCGTCGGGGTCTGTCTCTTGACTTCAGCATTTGTTGGCATGGCTTTTACTATCCAATTCGTTAGGGAATTTTCTAGATTAGGGTTAAGCAGAGCTGTTGGTGGGGTTTTAGCCCTGGCTTTCTCAAGGGAGTTGAGTCCTGTAATTACATCAATCGTGGTTGCTGGGCGTATTGGAAGTTCATTTGCAGCAGAGTTGGGAACAATGCAGGTTTCTGAGCAAACCGACACACTGAGAGTTCTCGGGGCAGACCCAGTTGATTATCTTGTGACTCCAAGAGTGATTGCTACTTGTATTGCTCTACCGTTTCTGACTCTAATGTGTTTCACAGTAGGGATGGCATCCAGCGCCCTCCTTTCCGATGGCATTTATGGTGTTAGCATCAACATTATTCTGGATTCAGCTTGCAGAGCTCTCAAGCCATGGGATATAATTAGTGCAATGATCAAGTCACAGGTTTTTGGTCCGATCATATCGATTGTGAGTTGTGCCTGGGGAGTTACCACTATGGGAGGAGCCAAGGGAGTTGGGGAGTCAACAACTTCGGCTGTGGTTATATCTCTTGTTGGGATCTTTATCGCAGATTTTGTACTCTCTTGTTGTTTCTTCCAAGGAGTTGGCGATTCATTGAAGAGGCTATGA

Protein Analysis

353

Amino Acids

37.95

Weight (kDa)

9.26

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MlaE PF02405 130 - 338 1.2e-68 Permease MlaE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 616
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 1 cut(s) 9
AclWI GGATC 1 cut(s) 995
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 3 cut(s) 182, 374, 426
AfaI GTAC 1 cut(s) 1008
AgsI TTSAA 4 cut(s) 99, 237, 361, 1048
AjnI CCWGG 3 cut(s) 412, 531, 908
AluBI AGCT 4 cut(s) 323, 512, 821, 830
AluI AGCT 4 cut(s) 323, 512, 821, 830
Alw21I GWGCWC 2 cut(s) 375, 832
Alw26I GTCTC 2 cut(s) 263, 437
Alw44I GTGCAC 1 cut(s) 371
AlwI GGATC 1 cut(s) 995
Ama87I CYCGRG 1 cut(s) 659
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 1 cut(s) 205
ApaLI GTGCAC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 1 cut(s) 485
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 2 cut(s) 410, 881
AsuHPI GGTGA 1 cut(s) 282
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 2 cut(s) 410, 881
BaeGI GKGCMC 1 cut(s) 375
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 1 cut(s) 832
Bbv12I GWGCWC 2 cut(s) 375, 832
BbvI GCAGC 1 cut(s) 617
BccI CCATC 4 cut(s) 197, 216, 748, 773
BciT130I CCWGG 3 cut(s) 414, 533, 910
BclI TGATCA 1 cut(s) 861
BcoDI GTCTC 2 cut(s) 263, 437
BfaI CTAG 1 cut(s) 492
BfoI RGCGCY 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 616
BisI GCNGC 1 cut(s) 606
BlsI GCNGC 1 cut(s) 607
Bme1390I CCNGG 3 cut(s) 414, 533, 910
Bme18I GGWCC 2 cut(s) 410, 881
BmeT110I CYCGRG 1 cut(s) 659
BmgT120I GGNCC 2 cut(s) 410, 881
BmiI GGNNCC 2 cut(s) 418, 934
BmrFI CCNGG 3 cut(s) 414, 533, 910
BmrI ACTGGG 3 cut(s) 249, 385, 665
BmsI GCATC 2 cut(s) 767, 807
BmuI ACTGGG 3 cut(s) 249, 385, 665
BoxI GACNNNNGTC 1 cut(s) 139
BpuEI CTTGAG 2 cut(s) 526, 818
Bsa29I ATCGAT 1 cut(s) 893
BsaAI YACGTR 1 cut(s) 300
BsaBI GATNNNNATC 2 cut(s) 207, 992
BsaJI CCNNGG 5 cut(s) 531, 839, 909, 936, 1027
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bse1I ACTGG 4 cut(s) 65, 255, 380, 671
Bse3DI GCAATG 1 cut(s) 864
Bse8I GATNNNNATC 2 cut(s) 207, 992
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 3 cut(s) 414, 533, 910
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 5 cut(s) 531, 839, 909, 936, 1027
BseGI GGATG 3 cut(s) 208, 758, 759
BseJI GATNNNNATC 2 cut(s) 207, 992
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 3 cut(s) 152, 624, 641
BseNI ACTGG 4 cut(s) 65, 255, 380, 671
BseRI GAGGAG 1 cut(s) 945
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 617
BseYI CCCAGC 1 cut(s) 581
BshFI GGCC 1 cut(s) 207
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 375, 832
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 1 cut(s) 260
BsmAI GTCTC 2 cut(s) 263, 437
BsmBI CGTCTC 1 cut(s) 263
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 375, 832
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 3 cut(s) 861, 885, 987
Bsp19I CCATGG 1 cut(s) 839
BspACI CCGC 1 cut(s) 9
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 3 cut(s) 151, 625, 642
BspDI ATCGAT 1 cut(s) 893
BspEI TCCGGA 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 418, 934
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 4 cut(s) 65, 255, 380, 671
BssECI CCNNGG 5 cut(s) 531, 839, 909, 936, 1027
BssMI GATC 3 cut(s) 861, 885, 987
BssT1I CCWWGG 3 cut(s) 839, 936, 1027
Bst2UI CCWGG 3 cut(s) 414, 533, 910
Bst4CI ACNGT 4 cut(s) 130, 274, 723, 748
Bst6I CTCTTC 1 cut(s) 1043
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 823
BstDEI CTNAG 3 cut(s) 138, 633, 650
BstDSI CCRYGG 1 cut(s) 839
BstEII GGTNACC 1 cut(s) 288
BstF5I GGATG 3 cut(s) 208, 758, 759
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 3 cut(s) 864, 888, 990
BstMAI GTCTC 2 cut(s) 263, 437
BstMBI GATC 3 cut(s) 861, 885, 987
BstMWI GCNNNNNNNGC 2 cut(s) 764, 827
BstNI CCWGG 3 cut(s) 414, 533, 910
BstPAI GACNNNNGTC 1 cut(s) 139
BstPI GGTNACC 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 412, 531, 908
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 987
BstXI CCANNNNNNTGG 2 cut(s) 72, 1034
BstYI RGATCY 1 cut(s) 987
Bsu15I ATCGAT 1 cut(s) 893
BsuRI GGCC 1 cut(s) 207
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 839
BtsCI GGATG 3 cut(s) 208, 758, 759
BtsIMutI CAGTG 2 cut(s) 373, 647
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 1 cut(s) 823
CfoI GCGC 1 cut(s) 767
Cfr13I GGNCC 2 cut(s) 410, 881
ClaI ATCGAT 1 cut(s) 893
CsiI ACCWGGT 1 cut(s) 412
Csp6I GTAC 1 cut(s) 1007
CviAII CATG 3 cut(s) 248, 457, 840
CviQI GTAC 1 cut(s) 1007
DdeI CTNAG 3 cut(s) 138, 633, 650
DpnI GATC 3 cut(s) 863, 887, 989
DpnII GATC 3 cut(s) 861, 885, 987
DraI TTTAAA 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 1043
EarI CTCTTC 1 cut(s) 1043
Ecl136II GAGCTC 1 cut(s) 830
Eco130I CCWWGG 3 cut(s) 839, 936, 1027
Eco24I GRGCYC 1 cut(s) 832
Eco47I GGWCC 2 cut(s) 410, 881
Eco53kI GAGCTC 1 cut(s) 830
Eco57I CTGAAG 3 cut(s) 182, 374, 426
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 830
EcoO65I GGTNACC 1 cut(s) 288
EcoRII CCWGG 3 cut(s) 412, 531, 908
EcoT14I CCWWGG 3 cut(s) 839, 936, 1027
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 3 cut(s) 839, 936, 1027
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 3 cut(s) 251, 460, 843
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 3 cut(s) 247, 456, 839
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 1 cut(s) 606
FokI GGATG 3 cut(s) 195, 745, 766
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 1 cut(s) 606
FspBI CTAG 1 cut(s) 492
GlaI GCGC 1 cut(s) 766
GluI GCNGC 1 cut(s) 606
GsaI CCCAGC 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 260
HhaI GCGC 1 cut(s) 767
Hin1II CATG 3 cut(s) 251, 460, 843
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 954
HindII GTYRAC 1 cut(s) 954
HinfI GANTC 7 cut(s) 233, 553, 688, 730, 815, 950, 1040
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 282
Hpy166II GTNNAC 2 cut(s) 373, 954
Hpy188I TCNGA 6 cut(s) 43, 123, 634, 729, 778, 885
Hpy188III TCNNGA 6 cut(s) 107, 260, 331, 436, 492, 812
Hpy8I GTNNAC 2 cut(s) 373, 954
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 2 cut(s) 222, 355
HpyCH4III ACNGT 4 cut(s) 130, 274, 723, 748
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 9 cut(s) 4, 185, 251, 280, 373, 605, 625, 825, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 764, 827
HpyF3I CTNAG 3 cut(s) 138, 633, 650
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 3 cut(s) 251, 460, 843
HspAI GCGC 1 cut(s) 765
Kpn2I TCCGGA 1 cut(s) 259
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 3 cut(s) 861, 885, 987
LmnI GCTCC 1 cut(s) 932
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 2 cut(s) 767, 807
MabI ACCWGGT 1 cut(s) 412
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 4 cut(s) 288, 685, 867, 916
MalI GATC 3 cut(s) 863, 887, 989
MboI GATC 3 cut(s) 861, 885, 987
MboII GAAGA 4 cut(s) 11, 60, 242, 1015
MflI RGATCY 1 cut(s) 987
MhlI GDGCHC 2 cut(s) 375, 832
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 5 cut(s) 395, 473, 485, 561, 849
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 4 cut(s) 562, 682, 724, 959
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 7 cut(s) 147, 229, 303, 303, 779, 923, 1044
Mox20I TGGCCA 1 cut(s) 207
MroI TCCGGA 1 cut(s) 259
MscI TGGCCA 1 cut(s) 207
MseI TTAA 4 cut(s) 59, 78, 398, 503
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 3 cut(s) 414, 533, 910
MvaI CCWGG 3 cut(s) 414, 533, 910
MwoI GCNNNNNNNGC 2 cut(s) 764, 827
NcoI CCATGG 1 cut(s) 839
NdeII GATC 3 cut(s) 861, 885, 987
NlaIII CATG 3 cut(s) 251, 460, 843
NlaIV GGNNCC 2 cut(s) 418, 934
NmuCI GTSAC 3 cut(s) 288, 685, 867
PfeI GAWTC 3 cut(s) 233, 815, 1040
PkrI GCNGC 1 cut(s) 607
PleI GAGTC 4 cut(s) 561, 682, 724, 958
PpsI GAGTC 4 cut(s) 561, 682, 724, 958
Ppu21I YACGTR 1 cut(s) 300
PshAI GACNNNNGTC 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 832
Psp6I CCWGG 3 cut(s) 412, 531, 908
PspEI GGTNACC 1 cut(s) 288
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 3 cut(s) 412, 531, 908
PspN4I GGNNCC 2 cut(s) 418, 934
PspPI GGNCC 2 cut(s) 410, 881
PsrI GAACNNNNNNTAC 2 cut(s) 580, 612
PsuI RGATCY 1 cut(s) 987
RsaI GTAC 1 cut(s) 1008
RsaNI GTAC 1 cut(s) 1007
SacI GAGCTC 1 cut(s) 832
SaqAI TTAA 4 cut(s) 59, 78, 398, 503
SatI GCNGC 1 cut(s) 606
Sau3AI GATC 3 cut(s) 861, 885, 987
Sau96I GGNCC 2 cut(s) 410, 881
SchI GAGTC 4 cut(s) 562, 682, 724, 959
ScrFI CCNGG 3 cut(s) 414, 533, 910
SduI GDGCHC 2 cut(s) 375, 832
SexAI ACCWGGT 1 cut(s) 412
SfaNI GCATC 2 cut(s) 767, 807
SinI GGWCC 2 cut(s) 410, 881
SmlI CTYRAG 2 cut(s) 541, 833
SmoI CTYRAG 2 cut(s) 541, 833
Sse9I AATT 5 cut(s) 395, 473, 485, 561, 849
SsiI CCGC 1 cut(s) 9
SspMI CTAG 1 cut(s) 492
SstI GAGCTC 1 cut(s) 832
StyD4I CCNGG 3 cut(s) 412, 531, 908
StyI CCWWGG 3 cut(s) 839, 936, 1027
TaaI ACNGT 4 cut(s) 130, 274, 723, 748
TaiI ACGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 893
TasI AATT 5 cut(s) 395, 473, 485, 561, 849
TatI WGTACW 1 cut(s) 1006
TfiI GAWTC 3 cut(s) 233, 815, 1040
Tru1I TTAA 4 cut(s) 59, 78, 398, 503
Tru9I TTAA 4 cut(s) 59, 78, 398, 503
TscAI CASTG 2 cut(s) 380, 654
TseFI GTSAC 3 cut(s) 288, 685, 867
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 3 cut(s) 288, 685, 867
TspDTI ATGAA 3 cut(s) 11, 588, 1032
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 2 cut(s) 380, 654
VneI GTGCAC 1 cut(s) 371
VpaK11BI GGWCC 2 cut(s) 410, 881
XapI RAATTY 1 cut(s) 485
XbaI TCTAGA 1 cut(s) 491
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.