Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004681

Physical Location & Seq

Genomic Coordinates Reverse (-)

7038527 .. 7040231

Gene Structure 1705 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G20330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGACTCTTTAACACTCTTCTTCACCGGTGCACTCGTCGCCGTCGGTATCTACTGGTTCCTCTGCGTTCTCGGTCCAGCAGAGCGTAAAGGCAAACGAGCCGTAGATCTCTCTGGTGGCTCAATCTCCGCCGAGAAAGTCCAAGACAACTACAAACAGTACTGGTCTTTCTTCCGCCGTCCAAAAGAAATCGAAACCGCCGAGAAAGTTCCAGACTTCGTCGACACATTCTACAATCTCGTCACCGACATATACGAGTGGGGATGGGGACAATCCTTCCACTTCTCACCATCAATCCCCGGAAAATCTCACAAAGACGCCACGCGCCTCCACGAAGAGATGGCCGTAGATCTGATCCAAGTCAAACCTGGTCAAAAGATCCTAGACGTCGGATGCGGTGTCGGCGGTCCGATGCGAGCGATTGCATCTCACTCGCGAGCTAACGTAGTCGGGATTACAATAAACGAGTATCAGGTGAACAGAGCTCGTCTCCACAATAAGAAAGCTGGTCTCGACGCGCTTTGCGAGGTCGTGTGTGGTAACTTCCTCCAGATGCCGTTCGATGACAACAGTTTCGACGGTGCTTATTCCATCGAAGCCACGTGTCACGCGCCGAAGCTGGAAGAAGTGTACGCAGAGATCTACAGGGTGTTGAAACCCGGATCTATGTATGTGTCGTACGAGTGGGTTACGACGGAGAAATTTAAGGCGGAGGATGACGAACACGTGGAGGTAATCCAAGGGATTGAGAGAGGCGATGCGTTACCAGGGCTTAGGGCTTACGTGGATATAGCTGAGACGGCTAAAAAGGTTGGGTTTGAGATAGTGAAGGAGAAGGATCTGGCGAGTCCACCGGCTGAGCCGTGGTGGACTAGGCTTAAGATGGGTAGGCTTGCTTATTGGAGGAATCACATTGTGGTTCAGATTTTGTCAGCGGTTGGAGTTGCTCCTAAAGGAACTGTTGATGTTCATGAGATGTTGTTTAAGACTGCTGATTATTTGACCAGAGGAGGTGAAACCGGAATATTCTCTCCGATGCATATGATTCTCTGCAGAAAACCGGAGTCACCGGAGGAGAGTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006275 GO:0006629 GO:0006694 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008156 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009825 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010051 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0012505 GO:0016049 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0045934 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090329 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko00100 ko01110 map00100 map01110

Pfam Domains

Protein Families

Methyltransf_11 Sterol_MT_C

Protein Analysis

361

Amino Acids

40.45

Weight (kDa)

5.75

Isoelectric Point (pI)

35.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	75 - 281	1.5e-15	Mycolic acid cyclopropane synthetase
Ubie_methyltran	PF01209	110 - 230	3.9e-11	ubiE/COQ5 methyltransferase family
Methyltransf_31	PF13847	122 - 236	4.1e-17	Methyltransferase domain
Methyltransf_23	PF13489	122 - 223	1.5e-06	Methyltransferase domain
Methyltransf_25	PF13649	127 - 221	5.9e-20	Methyltransferase domain
Methyltransf_11	PF08241	128 - 224	2.2e-23	Methyltransferase domain
Methyltransf_12	PF08242	128 - 223	4.2e-11	Methyltransferase domain
Sterol_MT_C	PF08498	291 - 355	2.7e-23	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20330 AT1G76090
fragaria_vesca	FvH4_2g37920
malus_domestica	MD08G1007400.v1.1 MD15G1006600.v1.1
prunus_persica	Prupe.1G359200_v2.0.a1
pyrus_communis	pycom08g00600 pycom15g00520
rosa_chinensis	RchiOBHm_Chr4g0386051 RchiOBHm_Chr6g0303691
rosa_laevigata	RLG00000010176 RLG00000011056
rosa_multiflora	Rmu_co8442993.1_g000001 Rmu_sc0000472.1_g000013 Rmu_sc0002413.1_g000008 Rmu_sc0026342.1_g000001 Rmu_ssc0000114.1_g000012
rosa_roxburghii	Rroxscaffold_5G00333810 Rroxscaffold_7G00164480
rosa_rugosa	Rorug03G0306500 Rorug06G0324000
rosa_samantha	Rh4AG010400 Rh4BG007500 Rh4CG011100 Rh4DG008800 Rh6AG438300 Rh6BG464800 Rh6CG450600 Rh6DG437200
rosa_wichuraiana	Rw4G000100 Rw6G037930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	390
AccI	GTMKAC	1 cut(s)	222
AccII	CGCG	4 cut(s)	325, 436, 518, 611
AciI	CCGC	7 cut(s)	129, 175, 198, 396, 405, 710, 935
AclWI	GGATC	4 cut(s)	349, 373, 670, 846
AcoI	YGGCCR	1 cut(s)	342
AcsI	RAATTY	1 cut(s)	701
AcvI	CACGTG	2 cut(s)	603, 727
AcyI	GRCGYC	2 cut(s)	318, 387
AdeI	CACNNNGTG	1 cut(s)	916
AfaI	GTAC	3 cut(s)	161, 632, 680
AflII	CTTAAG	1 cut(s)	878
AflIII	ACRYGT	2 cut(s)	602, 724
AgeI	ACCGGT	1 cut(s)	26
AgsI	TTSAA	1 cut(s)	655
AjnI	CCWGG	2 cut(s)	367, 766
AluBI	AGCT	5 cut(s)	440, 485, 506, 619, 794
AluI	AGCT	5 cut(s)	440, 485, 506, 619, 794
Alw21I	GWGCWC	2 cut(s)	34, 487
Alw26I	GTCTC	3 cut(s)	494, 515, 791
Alw44I	GTGCAC	1 cut(s)	30
AlwI	GGATC	4 cut(s)	349, 373, 670, 846
AoxI	GGCC	1 cut(s)	342
ApaLI	GTGCAC	1 cut(s)	30
ApoI	RAATTY	1 cut(s)	701
ArsI	GACNNNNNNTTYG	2 cut(s)	557, 589
AsiGI	ACCGGT	1 cut(s)	26
AspLEI	GCGC	3 cut(s)	327, 520, 613
AspS9I	GGNCC	2 cut(s)	74, 407
AsuC2I	CCSGG	2 cut(s)	300, 660
AsuHPI	GGTGA	6 cut(s)	16, 235, 279, 487, 1025, 1059
AvaII	GGWCC	2 cut(s)	74, 407
BaeGI	GKGCMC	1 cut(s)	34
BanII	GRGCYC	1 cut(s)	487
BbrPI	CACGTG	2 cut(s)	603, 727
Bbv12I	GWGCWC	2 cut(s)	34, 487
BccI	CCATC	5 cut(s)	258, 298, 334, 599, 877
BceAI	ACGGC	7 cut(s)	26, 86, 162, 329, 541, 816, 847
BcgI	CGANNNNNNTGC	2 cut(s)	414, 448
BciT130I	CCWGG	2 cut(s)	369, 768
BcnI	CCSGG	2 cut(s)	300, 660
BcoDI	GTCTC	3 cut(s)	494, 515, 791
BfaI	CTAG	2 cut(s)	383, 873
BfmI	CTRYAG	2 cut(s)	643, 1051
BfrI	CTTAAG	1 cut(s)	878
BglII	AGATCT	3 cut(s)	106, 349, 639
BlpI	GCTNAGC	1 cut(s)	858
BmcAI	AGTACT	1 cut(s)	161
Bme1390I	CCNGG	4 cut(s)	300, 369, 660, 768
Bme18I	GGWCC	2 cut(s)	74, 407
BmgT120I	GGNCC	2 cut(s)	74, 407
BmiI	GGNNCC	1 cut(s)	59
BmrFI	CCNGG	4 cut(s)	300, 369, 660, 768
BmsI	GCATC	6 cut(s)	383, 402, 434, 543, 748, 1026
BplI	GAGNNNNNCTC	2 cut(s)	474, 506
BpmI	CTGGAG	1 cut(s)	533
Bpu10I	CCTNAGC	1 cut(s)	773
Bpu1102I	GCTNAGC	1 cut(s)	858
BpuMI	CCSGG	2 cut(s)	300, 660
BsaAI	YACGTR	3 cut(s)	603, 727, 784
BsaHI	GRCGYC	2 cut(s)	318, 387
BsaI	GGTCTC	1 cut(s)	515
BsaJI	CCNNGG	4 cut(s)	298, 739, 767, 863
BsaWI	WCCGGW	4 cut(s)	26, 1019, 1060, 1069
Bse118I	RCCGGY	2 cut(s)	26, 853
Bse1I	ACTGG	2 cut(s)	59, 167
BseBI	CCWGG	2 cut(s)	369, 768
BseDI	CCNNGG	4 cut(s)	298, 739, 767, 863
BseGI	GGATG	3 cut(s)	269, 398, 721
BseMII	CTCAG	2 cut(s)	786, 849
BseNI	ACTGG	2 cut(s)	59, 167
BseRI	GAGGAG	1 cut(s)	1023
BseSI	GKGCMC	1 cut(s)	34
Bsh1236I	CGCG	4 cut(s)	325, 436, 518, 611
BshFI	GGCC	1 cut(s)	344
BshTI	ACCGGT	1 cut(s)	26
BsiHKAI	GWGCWC	2 cut(s)	34, 487
BsiSI	CCGG	7 cut(s)	27, 300, 660, 854, 1020, 1061, 1070
BsiWI	CGTACG	1 cut(s)	678
BslFI	GGGAC	1 cut(s)	282
BsmAI	GTCTC	3 cut(s)	494, 515, 791
BsmBI	CGTCTC	2 cut(s)	494, 791
BsmFI	GGGAC	1 cut(s)	282
BsnI	GGCC	1 cut(s)	344
Bso31I	GGTCTC	1 cut(s)	515
Bsp1286I	GDGCHC	2 cut(s)	34, 487
Bsp143I	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
Bsp1720I	GCTNAGC	1 cut(s)	858
Bsp68I	TCGCGA	1 cut(s)	436
BspACI	CCGC	7 cut(s)	129, 175, 198, 396, 405, 710, 935
BspANI	GGCC	1 cut(s)	344
BspCNI	CTCAG	2 cut(s)	787, 850
BspFNI	CGCG	4 cut(s)	325, 436, 518, 611
BspHI	TCATGA	1 cut(s)	970
BspLI	GGNNCC	1 cut(s)	59
BspMAI	CTGCAG	1 cut(s)	1055
BspPI	GGATC	4 cut(s)	349, 373, 670, 846
BspTI	CTTAAG	1 cut(s)	878
BspTNI	GGTCTC	1 cut(s)	515
BsrFI	RCCGGY	2 cut(s)	26, 853
BsrI	ACTGG	2 cut(s)	59, 167
BssAI	RCCGGY	2 cut(s)	26, 853
BssECI	CCNNGG	4 cut(s)	298, 739, 767, 863
BssMI	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
BssNI	GRCGYC	2 cut(s)	318, 387
BssT1I	CCWWGG	1 cut(s)	739
Bst2UI	CCWGG	2 cut(s)	369, 768
Bst4CI	ACNGT	4 cut(s)	159, 572, 581, 961
Bst6I	CTCTTC	2 cut(s)	23, 330
BstACI	GRCGYC	2 cut(s)	318, 387
BstAFI	CTTAAG	1 cut(s)	878
BstBAI	YACGTR	3 cut(s)	603, 727, 784
BstC8I	GCNNGC	3 cut(s)	417, 438, 894
BstDEI	CTNAG	3 cut(s)	773, 795, 858
BstDSI	CCRYGG	1 cut(s)	863
BstF5I	GGATG	3 cut(s)	269, 398, 721
BstFNI	CGCG	4 cut(s)	325, 436, 518, 611
BstHHI	GCGC	3 cut(s)	327, 520, 613
BstKTI	GATC	7 cut(s)	109, 352, 357, 381, 642, 665, 841
BstMAI	GTCTC	3 cut(s)	494, 515, 791
BstMBI	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
BstMWI	GCNNNNNNNGC	3 cut(s)	38, 402, 800
BstNI	CCWGG	2 cut(s)	369, 768
BstSCI	CCNGG	4 cut(s)	298, 367, 658, 766
BstSFI	CTRYAG	2 cut(s)	643, 1051
BstSLI	GKGCMC	1 cut(s)	34
BstUI	CGCG	4 cut(s)	325, 436, 518, 611
BstX2I	RGATCY	6 cut(s)	106, 349, 378, 639, 662, 838
BstYI	RGATCY	6 cut(s)	106, 349, 378, 639, 662, 838
BsuRI	GGCC	1 cut(s)	344
BtgI	CCRYGG	1 cut(s)	863
BtgZI	GCGATG	1 cut(s)	771
BtsCI	GGATG	3 cut(s)	269, 398, 721
BtuMI	TCGCGA	1 cut(s)	436
Cac8I	GCNNGC	3 cut(s)	417, 438, 894
CciI	TCATGA	1 cut(s)	970
CfoI	GCGC	3 cut(s)	327, 520, 613
Cfr10I	RCCGGY	2 cut(s)	26, 853
Cfr13I	GGNCC	2 cut(s)	74, 407
CpoI	CGGWCCG	1 cut(s)	407
CseI	GACGC	2 cut(s)	326, 524
CsiI	ACCWGGT	1 cut(s)	367
Csp6I	GTAC	3 cut(s)	160, 631, 679
CspAI	ACCGGT	1 cut(s)	26
CspI	CGGWCCG	1 cut(s)	407
CviAII	CATG	1 cut(s)	971
CviQI	GTAC	3 cut(s)	160, 631, 679
DdeI	CTNAG	3 cut(s)	773, 795, 858
DpnI	GATC	7 cut(s)	108, 351, 356, 380, 641, 664, 840
DpnII	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
DraIII	CACNNNGTG	1 cut(s)	916
EaeI	YGGCCR	1 cut(s)	342
Eam1104I	CTCTTC	2 cut(s)	23, 330
EarI	CTCTTC	2 cut(s)	23, 330
EciI	GGCGGA	3 cut(s)	118, 164, 725
Ecl136II	GAGCTC	1 cut(s)	485
Eco130I	CCWWGG	1 cut(s)	739
Eco24I	GRGCYC	1 cut(s)	487
Eco31I	GGTCTC	1 cut(s)	515
Eco47I	GGWCC	2 cut(s)	74, 407
Eco53kI	GAGCTC	1 cut(s)	485
Eco72I	CACGTG	2 cut(s)	603, 727
EcoICRI	GAGCTC	1 cut(s)	485
EcoRII	CCWGG	2 cut(s)	367, 766
EcoT14I	CCWWGG	1 cut(s)	739
EcoT22I	ATGCAT	1 cut(s)	1041
EcoT38I	GRGCYC	1 cut(s)	487
ErhI	CCWWGG	1 cut(s)	739
Esp3I	CGTCTC	2 cut(s)	494, 791
FaeI	CATG	1 cut(s)	974
FaiI	YATR	8 cut(s)	251, 253, 668, 672, 791, 972, 1041, 1043
FaqI	GGGAC	1 cut(s)	282
FatI	CATG	1 cut(s)	970
FauNDI	CATATG	1 cut(s)	1041
FblI	GTMKAC	1 cut(s)	222
FokI	GGATG	3 cut(s)	276, 405, 728
FriOI	GRGCYC	1 cut(s)	487
FspBI	CTAG	2 cut(s)	383, 873
GlaI	GCGC	3 cut(s)	326, 519, 612
GsuI	CTGGAG	1 cut(s)	533
HaeIII	GGCC	1 cut(s)	344
HapII	CCGG	7 cut(s)	27, 300, 660, 854, 1020, 1061, 1070
HgaI	GACGC	2 cut(s)	326, 524
HhaI	GCGC	3 cut(s)	327, 520, 613
Hin1I	GRCGYC	2 cut(s)	318, 387
Hin1II	CATG	1 cut(s)	974
Hin6I	GCGC	3 cut(s)	325, 518, 611
HinP1I	GCGC	3 cut(s)	325, 518, 611
HincII	GTYRAC	1 cut(s)	223
HindII	GTYRAC	1 cut(s)	223
HinfI	GANTC	5 cut(s)	5, 847, 907, 1045, 1064
HpaII	CCGG	7 cut(s)	27, 300, 660, 854, 1020, 1061, 1070
HphI	GGTGA	6 cut(s)	16, 235, 279, 487, 1025, 1059
Hpy166II	GTNNAC	6 cut(s)	32, 223, 478, 631, 851, 870
Hpy188I	TCNGA	5 cut(s)	354, 392, 411, 924, 1035
Hpy188III	TCNNGA	7 cut(s)	212, 435, 451, 512, 550, 971, 1083
Hpy8I	GTNNAC	6 cut(s)	32, 223, 478, 631, 851, 870
Hpy99I	CGWCG	7 cut(s)	41, 47, 224, 392, 518, 581, 697
HpyAV	CCTTC	3 cut(s)	286, 823, 829
HpyCH4III	ACNGT	4 cut(s)	159, 572, 581, 961
HpyCH4IV	ACGT	5 cut(s)	387, 444, 602, 726, 783
HpyCH4V	TGCA	4 cut(s)	32, 425, 1039, 1053
HpyF10VI	GCNNNNNNNGC	3 cut(s)	38, 402, 800
HpyF3I	CTNAG	3 cut(s)	773, 795, 858
HpySE526I	ACGT	5 cut(s)	387, 444, 602, 726, 783
Hsp92I	GRCGYC	2 cut(s)	318, 387
Hsp92II	CATG	1 cut(s)	974
HspAI	GCGC	3 cut(s)	325, 518, 611
Kzo9I	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
LmnI	GCTCC	1 cut(s)	952
LweI	GCATC	6 cut(s)	383, 402, 434, 543, 748, 1026
MabI	ACCWGGT	1 cut(s)	367
MaeI	CTAG	2 cut(s)	383, 873
MaeII	ACGT	5 cut(s)	387, 444, 602, 726, 783
MaeIII	GTNAC	6 cut(s)	241, 539, 605, 688, 762, 1065
MalI	GATC	7 cut(s)	108, 351, 356, 380, 641, 664, 840
MboI	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
MboII	GAAGA	5 cut(s)	10, 13, 163, 347, 635
MflI	RGATCY	6 cut(s)	106, 349, 378, 639, 662, 838
MhlI	GDGCHC	2 cut(s)	34, 487
MluCI	AATT	1 cut(s)	701
MlyI	GAGTC	2 cut(s)	856, 1073
MmeI	TCCRAC	2 cut(s)	370, 919
Mph1103I	ATGCAT	1 cut(s)	1041
MseI	TTAA	4 cut(s)	11, 705, 879, 984
MspA1I	CMGCKG	1 cut(s)	935
MspCI	CTTAAG	1 cut(s)	878
MspI	CCGG	7 cut(s)	27, 300, 660, 854, 1020, 1061, 1070
MspR9I	CCNGG	4 cut(s)	300, 369, 660, 768
MvaI	CCWGG	2 cut(s)	369, 768
MvnI	CGCG	4 cut(s)	325, 436, 518, 611
MwoI	GCNNNNNNNGC	3 cut(s)	38, 402, 800
NciI	CCSGG	2 cut(s)	300, 660
NdeI	CATATG	1 cut(s)	1041
NdeII	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
NlaIII	CATG	1 cut(s)	974
NlaIV	GGNNCC	1 cut(s)	59
NmeAIII	GCCGAG	2 cut(s)	157, 226
NmuCI	GTSAC	3 cut(s)	241, 605, 1065
NruI	TCGCGA	1 cut(s)	436
NsiI	ATGCAT	1 cut(s)	1041
PagI	TCATGA	1 cut(s)	970
PcsI	WCGNNNNNNNCGW	2 cut(s)	198, 522
PfeI	GAWTC	2 cut(s)	907, 1045
Pfl23II	CGTACG	1 cut(s)	678
PflFI	GACNNNGTC	1 cut(s)	218
PinAI	ACCGGT	1 cut(s)	26
PleI	GAGTC	2 cut(s)	855, 1072
PmaCI	CACGTG	2 cut(s)	603, 727
PmlI	CACGTG	2 cut(s)	603, 727
PpsI	GAGTC	2 cut(s)	855, 1072
Ppu21I	YACGTR	3 cut(s)	603, 727, 784
Psp124BI	GAGCTC	1 cut(s)	487
Psp6I	CCWGG	2 cut(s)	367, 766
PspCI	CACGTG	2 cut(s)	603, 727
PspGI	CCWGG	2 cut(s)	367, 766
PspLI	CGTACG	1 cut(s)	678
PspN4I	GGNNCC	1 cut(s)	59
PspPI	GGNCC	2 cut(s)	74, 407
PstI	CTGCAG	1 cut(s)	1055
PsuI	RGATCY	6 cut(s)	106, 349, 378, 639, 662, 838
PsyI	GACNNNGTC	1 cut(s)	218
RruI	TCGCGA	1 cut(s)	436
RsaI	GTAC	3 cut(s)	161, 632, 680
RsaNI	GTAC	3 cut(s)	160, 631, 679
Rsr2I	CGGWCCG	1 cut(s)	407
RsrII	CGGWCCG	1 cut(s)	407
SacI	GAGCTC	1 cut(s)	487
SalI	GTCGAC	1 cut(s)	221
SaqAI	TTAA	4 cut(s)	11, 705, 879, 984
Sau3AI	GATC	7 cut(s)	106, 349, 354, 378, 639, 662, 838
Sau96I	GGNCC	2 cut(s)	74, 407
ScaI	AGTACT	1 cut(s)	161
SchI	GAGTC	2 cut(s)	856, 1073
ScrFI	CCNGG	4 cut(s)	300, 369, 660, 768
SduI	GDGCHC	2 cut(s)	34, 487
SexAI	ACCWGGT	1 cut(s)	367
SfaNI	GCATC	6 cut(s)	383, 402, 434, 543, 748, 1026
SfcI	CTRYAG	2 cut(s)	643, 1051
SgrAI	CRCCGGYG	1 cut(s)	26
SinI	GGWCC	2 cut(s)	74, 407
SmlI	CTYRAG	1 cut(s)	878
SmoI	CTYRAG	1 cut(s)	878
Sse9I	AATT	1 cut(s)	701
SsiI	CCGC	7 cut(s)	129, 175, 198, 396, 405, 710, 935
SspI	AATATT	1 cut(s)	1026
SspMI	CTAG	2 cut(s)	383, 873
SstI	GAGCTC	1 cut(s)	487
StyD4I	CCNGG	4 cut(s)	298, 367, 658, 766
StyI	CCWWGG	1 cut(s)	739
TaaI	ACNGT	4 cut(s)	159, 572, 581, 961
TaiI	ACGT	5 cut(s)	390, 447, 605, 729, 786
TaqI	TCGA	6 cut(s)	192, 222, 513, 561, 576, 594
TaqII	GACCGA	1 cut(s)	62
TasI	AATT	1 cut(s)	701
TatI	WGTACW	1 cut(s)	159
TfiI	GAWTC	2 cut(s)	907, 1045
Tru1I	TTAA	4 cut(s)	11, 705, 879, 984
Tru9I	TTAA	4 cut(s)	11, 705, 879, 984
TseFI	GTSAC	3 cut(s)	241, 605, 1065
Tsp45I	GTSAC	3 cut(s)	241, 605, 1065
TspDTI	ATGAA	1 cut(s)	959
TspGWI	ACGGA	1 cut(s)	710
Tth111I	GACNNNGTC	1 cut(s)	218
Vha464I	CTTAAG	1 cut(s)	878
VneI	GTGCAC	1 cut(s)	30
VpaK11BI	GGWCC	2 cut(s)	74, 407
XapI	RAATTY	1 cut(s)	701
XcmI	CCANNNNNNNNNTGG	1 cut(s)	365
XmiI	GTMKAC	1 cut(s)	222
XspI	CTAG	2 cut(s)	383, 873
ZraI	GACGTC	1 cut(s)	388
ZrmI	AGTACT	1 cut(s)	161
Zsp2I	ATGCAT	1 cut(s)	1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.