Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0004681

Physical Location & Seq

Genomic Coordinates Forward (+)

307506 .. 308594

Gene Structure 1089 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g00520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGGACTCGCTCACTCTCTTTTGTACGGGCGCTCTCCTCGCCGGCGGGATCTATTGGTTTGTCTGCATTCTTGGCCCTGCCGAGCGAAAAGGCAAACGTGCCATGGATCTCTCTGGTGGCTCCATCTCCGCCGAGAAAGTTCAAGATAGCTACGACAAATATTGGTCCTTTTTCCGCCGCCCAAAAGAGATCGAAACGGCCGAGAAAGTCCCCGACTTTGTGGACACCTTTTACAATTTAGTCACCGATATTTACGAGTGGGGTTGGGGCCAGTCCTTTCACTTCTCCCCGCACGTCCCCGGCAAGTCCCACAAGGACGCCACGCGCCTCCACGAGGAGATGGCCGTCGATCTCATCGACGTCAAACCCGGAGACAGAATTCTCGACGTCGGATGCGGCGTAGGTGGGCCTATGCGTGCCATCGCGGCCCACTCGCGTGCCAACGTCACGGGGATCACGATCAACGAGTACCAGGTTAAACGGGCGCGTCAGCACAATAAGAAGGCCGGCCTCGACTCGCTCTGCGAGGTCGTCTGCGGGAACTTCCTCGAGATGCCGTTCCCGGAGAACAGCTTTGACGGCGCGTACTCAATCGAGGCGACATGCCACGCGCCGAAGCTGGAGGAGGTGTACGCGCAACACGAGATCTTCAAGGTTCTGAAGCCGGGGGCTCTGTACGTGTCGTACGAGTGGGTGACGACGGACAAGTACAAGGGTGACGACGCCGAACACGTGGATGTCATTCAGGGGATCGAGAGAGGGGACGCGCTGCCGGGACTCAGGAGCTATACGGATATTGCGGAGACGGCGAGGAAGGTCGGGTTCGAGGTGGTGAAGGAGAAAGATCTGGCCAAGCCGCCGGCGCATCCGTGGTGGAATCGATTGAAGATGGGGAGGATCGCATACTGGCGCAACCACATTCTGGTGACGGTGCTCGCCGCCGTCGGAATTGCACCGAAAGGAACCGTGGATGTGCACGAGATGCTGTTCAAAACCGCCGACTTTTTGACCAGGGGCGGCGAAACCGGAATCTTCAGTCCGATGCACATGATTCTCTGCCGGAAGCCCGAGACAGCCAAATCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006275 GO:0006629 GO:0006694 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008156 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009825 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010051 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0012505 GO:0016049 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0045934 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090329 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko00100 ko01110 map00100 map01110

Pfam Domains

Protein Families

Methyltransf_11 Sterol_MT_C

Protein Analysis

363

Amino Acids

40.45

Weight (kDa)

6.36

Isoelectric Point (pI)

32.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	76 - 284	1.1e-15	Mycolic acid cyclopropane synthetase
Ubie_methyltran	PF01209	110 - 230	1.4e-08	ubiE/COQ5 methyltransferase family
Methyltransf_31	PF13847	122 - 231	3.2e-15	Methyltransferase domain
Methyltransf_23	PF13489	122 - 279	4.2e-07	Methyltransferase domain
Methyltransf_25	PF13649	127 - 223	1.8e-18	Methyltransferase domain
Methyltransf_11	PF08241	128 - 226	3.2e-20	Methyltransferase domain
Methyltransf_12	PF08242	128 - 225	5.4e-10	Methyltransferase domain
Sterol_MT_C	PF08498	294 - 357	9.9e-23	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20330 AT1G76090
fragaria_vesca	FvH4_2g37920
malus_domestica	MD08G1007400.v1.1 MD15G1006600.v1.1
prunus_persica	Prupe.1G359200_v2.0.a1
pyrus_communis	pycom08g00600 pycom15g00520
rosa_chinensis	RchiOBHm_Chr4g0386051 RchiOBHm_Chr6g0303691
rosa_laevigata	RLG00000010176 RLG00000011056
rosa_multiflora	Rmu_co8442993.1_g000001 Rmu_sc0000472.1_g000013 Rmu_sc0002413.1_g000008 Rmu_sc0026342.1_g000001 Rmu_ssc0000114.1_g000012
rosa_roxburghii	Rroxscaffold_5G00333810 Rroxscaffold_7G00164480
rosa_rugosa	Rorug03G0306500 Rorug06G0324000
rosa_samantha	Rh4AG010400 Rh4BG007500 Rh4CG011100 Rh4DG008800 Rh6AG438300 Rh6BG464800 Rh6CG450600 Rh6DG437200
rosa_wichuraiana	Rw4G000100 Rw6G037930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	363, 390
AccB7I	CCANNNNNTGG	1 cut(s)	922
AccII	CGCG	8 cut(s)	325, 425, 436, 487, 584, 611, 635, 767
AclWI	GGATC	5 cut(s)	56, 114, 461, 758, 905
AcoI	YGGCCR	3 cut(s)	198, 342, 849
AcsI	RAATTY	1 cut(s)	378
AcuI	CTGAAG	2 cut(s)	680, 1018
AcvI	CACGTG	1 cut(s)	733
AcyI	GRCGYC	4 cut(s)	318, 360, 387, 723
AfaI	GTAC	7 cut(s)	25, 470, 587, 632, 677, 686, 710
AfiI	CCNNNNNNNGG	2 cut(s)	334, 922
AflIII	ACRYGT	2 cut(s)	678, 730
AgsI	TTSAA	4 cut(s)	143, 652, 886, 991
AjiI	CACGTC	1 cut(s)	295
AjnI	CCWGG	2 cut(s)	471, 1010
AleI	CACNNNNGTG	1 cut(s)	435
AluBI	AGCT	4 cut(s)	150, 573, 619, 786
AluI	AGCT	4 cut(s)	150, 573, 619, 786
Alw21I	GWGCWC	2 cut(s)	936, 978
Alw26I	GTCTC	3 cut(s)	366, 797, 1064
Alw44I	GTGCAC	1 cut(s)	974
AlwI	GGATC	5 cut(s)	56, 114, 461, 758, 905
Ama87I	CYCGRG	2 cut(s)	548, 1067
AoxI	GGCC	9 cut(s)	73, 198, 268, 342, 407, 426, 504, 508, 849
ApaLI	GTGCAC	1 cut(s)	974
ApeKI	GCWGC	1 cut(s)	769
ApoI	RAATTY	1 cut(s)	378
AspLEI	GCGC	9 cut(s)	32, 327, 487, 584, 613, 637, 769, 865, 912
AspS9I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
AsuC2I	CCSGG	5 cut(s)	300, 369, 563, 666, 774
AsuHPI	GGTGA	5 cut(s)	235, 706, 728, 844, 937
AvaI	CYCGRG	2 cut(s)	548, 1067
AvaII	GGWCC	1 cut(s)	165
BaeGI	GKGCMC	1 cut(s)	978
BalI	TGGCCA	1 cut(s)	851
BanII	GRGCYC	1 cut(s)	673
BauI	CACGAG	3 cut(s)	332, 641, 977
BbrPI	CACGTG	1 cut(s)	733
Bbv12I	GWGCWC	2 cut(s)	936, 978
BbvI	GCAGC	1 cut(s)	756
BccI	CCATC	4 cut(s)	131, 334, 428, 883
BceAI	ACGGC	6 cut(s)	213, 329, 541, 595, 822, 926
BciT130I	CCWGG	2 cut(s)	473, 1012
BcnI	CCSGG	5 cut(s)	300, 369, 563, 666, 774
BcoDI	GTCTC	3 cut(s)	366, 797, 1064
BfoI	RGCGCY	1 cut(s)	33
BglI	GCCNNNNNGGC	1 cut(s)	425
BglII	AGATCT	2 cut(s)	645, 844
BisI	GCNGC	7 cut(s)	178, 397, 426, 770, 857, 939, 1018
BlsI	GCNGC	7 cut(s)	179, 398, 427, 771, 858, 940, 1019
Bme1390I	CCNGG	7 cut(s)	300, 369, 473, 563, 666, 774, 1012
Bme18I	GGWCC	1 cut(s)	165
BmeT110I	CYCGRG	2 cut(s)	548, 1067
BmgBI	CACGTC	1 cut(s)	295
BmgT120I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
BmiI	GGNNCC	3 cut(s)	121, 269, 964
BmrFI	CCNGG	7 cut(s)	300, 369, 473, 563, 666, 774, 1012
BmsI	GCATC	5 cut(s)	383, 543, 874, 972, 1032
BpmI	CTGGAG	1 cut(s)	641
BpuMI	CCSGG	5 cut(s)	300, 369, 563, 666, 774
Bsa29I	ATCGAT	1 cut(s)	880
BsaAI	YACGTR	2 cut(s)	679, 733
BsaBI	GATNNNNATC	1 cut(s)	458
BsaHI	GRCGYC	4 cut(s)	318, 360, 387, 723
BsaJI	CCNNGG	6 cut(s)	102, 298, 665, 869, 966, 1011
BsaWI	WCCGGW	1 cut(s)	1025
BsaXI	ACNNNNNCTCC	2 cut(s)	614, 644
Bsc4I	CCNNNNNNNGG	2 cut(s)	334, 922
Bse118I	RCCGGY	3 cut(s)	41, 506, 859
Bse1I	ACTGG	2 cut(s)	271, 911
Bse8I	GATNNNNATC	1 cut(s)	458
BseBI	CCWGG	2 cut(s)	473, 1012
BseCI	ATCGAT	1 cut(s)	880
BseDI	CCNNGG	6 cut(s)	102, 298, 665, 869, 966, 1011
BseGI	GGATG	4 cut(s)	398, 742, 865, 976
BseJI	GATNNNNATC	1 cut(s)	458
BseLI	CCNNNNNNNGG	2 cut(s)	334, 922
BseMII	CTCAG	1 cut(s)	793
BseNI	ACTGG	2 cut(s)	271, 911
BseRI	GAGGAG	3 cut(s)	26, 350, 638
BseSI	GKGCMC	1 cut(s)	978
BseX3I	CGGCCG	1 cut(s)	198
BseXI	GCAGC	1 cut(s)	756
Bsh1236I	CGCG	8 cut(s)	325, 425, 436, 487, 584, 611, 635, 767
Bsh1285I	CGRYCG	1 cut(s)	201
BshFI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 851
BshVI	ATCGAT	1 cut(s)	880
BsiEI	CGRYCG	1 cut(s)	201
BsiHKAI	GWGCWC	2 cut(s)	936, 978
BsiHKCI	CYCGRG	2 cut(s)	548, 1067
BsiWI	CGTACG	1 cut(s)	684
BslFI	GGGAC	5 cut(s)	194, 281, 292, 776, 789
BslI	CCNNNNNNNGG	2 cut(s)	334, 922
BsmAI	GTCTC	3 cut(s)	366, 797, 1064
BsmBI	CGTCTC	1 cut(s)	797
BsmFI	GGGAC	5 cut(s)	194, 281, 292, 776, 789
BsmI	GAATGC	1 cut(s)	66
BsnI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 851
BsoBI	CYCGRG	2 cut(s)	548, 1067
Bsp1286I	GDGCHC	3 cut(s)	673, 936, 978
Bsp19I	CCATGG	1 cut(s)	102
BspANI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 851
BspCNI	CTCAG	1 cut(s)	792
BspDI	ATCGAT	1 cut(s)	880
BspFNI	CGCG	8 cut(s)	325, 425, 436, 487, 584, 611, 635, 767
BspLI	GGNNCC	3 cut(s)	121, 269, 964
BspPI	GGATC	5 cut(s)	56, 114, 461, 758, 905
BsrFI	RCCGGY	3 cut(s)	41, 506, 859
BsrI	ACTGG	2 cut(s)	271, 911
BssAI	RCCGGY	3 cut(s)	41, 506, 859
BssECI	CCNNGG	6 cut(s)	102, 298, 665, 869, 966, 1011
BssNI	GRCGYC	4 cut(s)	318, 360, 387, 723
BssSI	CACGAG	3 cut(s)	332, 641, 977
BssT1I	CCWWGG	1 cut(s)	102
Bst2BI	CACGAG	3 cut(s)	332, 641, 977
Bst2UI	CCWGG	2 cut(s)	473, 1012
Bst4CI	ACNGT	2 cut(s)	931, 967
BstACI	GRCGYC	4 cut(s)	318, 360, 387, 723
BstAPI	GCANNNNNTGC	1 cut(s)	982
BstBAI	YACGTR	2 cut(s)	679, 733
BstC8I	GCNNGC	6 cut(s)	43, 417, 438, 508, 861, 936
BstDEI	CTNAG	1 cut(s)	779
BstDSI	CCRYGG	3 cut(s)	102, 869, 966
BstENI	CCTNNNNNAGG	1 cut(s)	332
BstF5I	GGATG	4 cut(s)	398, 742, 865, 976
BstFNI	CGCG	8 cut(s)	325, 425, 436, 487, 584, 611, 635, 767
BstH2I	RGCGCY	1 cut(s)	33
BstHHI	GCGC	9 cut(s)	32, 327, 487, 584, 613, 637, 769, 865, 912
BstMAI	GTCTC	3 cut(s)	366, 797, 1064
BstMCI	CGRYCG	1 cut(s)	201
BstMWI	GCNNNNNNNGC	7 cut(s)	38, 72, 425, 579, 806, 862, 982
BstNI	CCWGG	2 cut(s)	473, 1012
BstNSI	RCATGY	1 cut(s)	606
BstSCI	CCNGG	7 cut(s)	298, 367, 471, 561, 664, 772, 1010
BstSLI	GKGCMC	1 cut(s)	978
BstUI	CGCG	8 cut(s)	325, 425, 436, 487, 584, 611, 635, 767
BstV1I	GCAGC	1 cut(s)	756
BstX2I	RGATCY	4 cut(s)	48, 106, 645, 844
BstYI	RGATCY	4 cut(s)	48, 106, 645, 844
BstZI	CGGCCG	1 cut(s)	198
Bsu15I	ATCGAT	1 cut(s)	880
BsuRI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 851
BsuTUI	ATCGAT	1 cut(s)	880
BtgI	CCRYGG	3 cut(s)	102, 869, 966
BtgZI	GCGATG	1 cut(s)	406
BtrI	CACGTC	1 cut(s)	295
BtsCI	GGATG	4 cut(s)	398, 742, 865, 976
Cac8I	GCNNGC	6 cut(s)	43, 417, 438, 508, 861, 936
CfoI	GCGC	9 cut(s)	32, 327, 487, 584, 613, 637, 769, 865, 912
Cfr10I	RCCGGY	3 cut(s)	41, 506, 859
Cfr13I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
ClaI	ATCGAT	1 cut(s)	880
CseI	GACGC	4 cut(s)	326, 476, 731, 773
CsiI	ACCWGGT	1 cut(s)	471
Csp6I	GTAC	7 cut(s)	24, 469, 586, 631, 676, 685, 709
CviAII	CATG	3 cut(s)	103, 603, 1048
CviQI	GTAC	7 cut(s)	24, 469, 586, 631, 676, 685, 709
DdeI	CTNAG	1 cut(s)	779
EaeI	YGGCCR	3 cut(s)	198, 342, 849
EagI	CGGCCG	1 cut(s)	198
EciI	GGCGGA	2 cut(s)	118, 164
EclXI	CGGCCG	1 cut(s)	198
Eco130I	CCWWGG	1 cut(s)	102
Eco24I	GRGCYC	1 cut(s)	673
Eco47I	GGWCC	1 cut(s)	165
Eco52I	CGGCCG	1 cut(s)	198
Eco57I	CTGAAG	2 cut(s)	680, 1018
Eco72I	CACGTG	1 cut(s)	733
Eco88I	CYCGRG	2 cut(s)	548, 1067
EcoNI	CCTNNNNNAGG	1 cut(s)	332
EcoRI	GAATTC	1 cut(s)	378
EcoRII	CCWGG	2 cut(s)	471, 1010
EcoT14I	CCWWGG	1 cut(s)	102
EcoT38I	GRGCYC	1 cut(s)	673
ErhI	CCWWGG	1 cut(s)	102
Esp3I	CGTCTC	1 cut(s)	797
FaeI	CATG	3 cut(s)	106, 606, 1051
FaiI	YATR	6 cut(s)	104, 413, 604, 789, 904, 1049
FaqI	GGGAC	5 cut(s)	194, 281, 292, 776, 789
FatI	CATG	3 cut(s)	102, 602, 1047
FauI	CCCGC	3 cut(s)	38, 297, 530
Fnu4HI	GCNGC	7 cut(s)	178, 397, 426, 770, 857, 939, 1018
FokI	GGATG	4 cut(s)	405, 749, 852, 983
FriOI	GRGCYC	1 cut(s)	673
FseI	GGCCGGCC	1 cut(s)	510
Fsp4HI	GCNGC	7 cut(s)	178, 397, 426, 770, 857, 939, 1018
GlaI	GCGC	9 cut(s)	31, 326, 486, 583, 612, 636, 768, 864, 911
GluI	GCNGC	7 cut(s)	178, 397, 426, 770, 857, 939, 1018
GsuI	CTGGAG	1 cut(s)	641
HaeII	RGCGCY	1 cut(s)	33
HaeIII	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 851
HgaI	GACGC	4 cut(s)	326, 476, 731, 773
HhaI	GCGC	9 cut(s)	32, 327, 487, 584, 613, 637, 769, 865, 912
Hin1I	GRCGYC	4 cut(s)	318, 360, 387, 723
Hin1II	CATG	3 cut(s)	106, 606, 1051
Hin6I	GCGC	9 cut(s)	30, 325, 485, 582, 611, 635, 767, 863, 910
HinP1I	GCGC	9 cut(s)	30, 325, 485, 582, 611, 635, 767, 863, 910
HinfI	GANTC	6 cut(s)	5, 515, 777, 877, 1029, 1051
HphI	GGTGA	5 cut(s)	235, 706, 728, 844, 937
Hpy166II	GTNNAC	3 cut(s)	223, 631, 976
Hpy188I	TCNGA	4 cut(s)	392, 660, 947, 1041
Hpy188III	TCNNGA	7 cut(s)	143, 383, 457, 550, 754, 781, 1086
Hpy8I	GTNNAC	3 cut(s)	223, 631, 976
Hpy99I	CGWCG	7 cut(s)	350, 362, 389, 392, 703, 725, 947
HpyAV	CCTTC	3 cut(s)	496, 808, 829
HpyCH4III	ACNGT	2 cut(s)	931, 967
HpyCH4IV	ACGT	7 cut(s)	97, 294, 360, 387, 444, 678, 732
HpyCH4V	TGCA	4 cut(s)	66, 953, 976, 1045
HpyF10VI	GCNNNNNNNGC	7 cut(s)	38, 72, 425, 579, 806, 862, 982
HpyF3I	CTNAG	1 cut(s)	779
HpySE526I	ACGT	7 cut(s)	97, 294, 360, 387, 444, 678, 732
Hsp92I	GRCGYC	4 cut(s)	318, 360, 387, 723
Hsp92II	CATG	3 cut(s)	106, 606, 1051
HspAI	GCGC	9 cut(s)	30, 325, 485, 582, 611, 635, 767, 863, 910
KroI	GCCGGC	3 cut(s)	41, 506, 859
KroNI	GCCGGC	3 cut(s)	43, 508, 861
LmnI	GCTCC	2 cut(s)	125, 783
Lsp1109I	GCAGC	1 cut(s)	756
LweI	GCATC	5 cut(s)	383, 543, 874, 972, 1032
MabI	ACCWGGT	1 cut(s)	471
MaeII	ACGT	7 cut(s)	97, 294, 360, 387, 444, 678, 732
MaeIII	GTNAC	5 cut(s)	241, 445, 694, 716, 925
MboII	GAAGA	3 cut(s)	640, 898, 1024
MflI	RGATCY	4 cut(s)	48, 106, 645, 844
MhlI	GDGCHC	3 cut(s)	673, 936, 978
MlsI	TGGCCA	1 cut(s)	851
MluCI	AATT	3 cut(s)	235, 378, 948
MluNI	TGGCCA	1 cut(s)	851
MlyI	GAGTC	2 cut(s)	509, 771
MmeI	TCCRAC	2 cut(s)	370, 925
Mox20I	TGGCCA	1 cut(s)	851
MreI	CGCCGGCG	2 cut(s)	41, 859
MroNI	GCCGGC	3 cut(s)	41, 506, 859
MscI	TGGCCA	1 cut(s)	851
MseI	TTAA	1 cut(s)	477
MslI	CAYNNNNRTG	3 cut(s)	435, 735, 923
Msp20I	TGGCCA	1 cut(s)	851
MspR9I	CCNGG	7 cut(s)	300, 369, 473, 563, 666, 774, 1012
Mva1269I	GAATGC	1 cut(s)	66
MvaI	CCWGG	2 cut(s)	473, 1012
MvnI	CGCG	8 cut(s)	325, 425, 436, 487, 584, 611, 635, 767
MwoI	GCNNNNNNNGC	7 cut(s)	38, 72, 425, 579, 806, 862, 982
NaeI	GCCGGC	3 cut(s)	43, 508, 861
NciI	CCSGG	5 cut(s)	300, 369, 563, 666, 774
NcoI	CCATGG	1 cut(s)	102
NgoMIV	GCCGGC	3 cut(s)	41, 506, 859
NlaIII	CATG	3 cut(s)	106, 606, 1051
NlaIV	GGNNCC	3 cut(s)	121, 269, 964
NmeAIII	GCCGAG	3 cut(s)	106, 157, 226
NmuCI	GTSAC	5 cut(s)	241, 445, 694, 716, 925
NspI	RCATGY	1 cut(s)	606
OliI	CACNNNNGTG	1 cut(s)	435
PaeR7I	CTCGAG	1 cut(s)	548
PcsI	WCGNNNNNNNCGW	7 cut(s)	198, 354, 396, 455, 639, 684, 729
PctI	GAATGC	1 cut(s)	66
PdiI	GCCGGC	3 cut(s)	43, 508, 861
PfeI	GAWTC	3 cut(s)	877, 1029, 1051
Pfl23II	CGTACG	1 cut(s)	684
PflMI	CCANNNNNTGG	1 cut(s)	922
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	7 cut(s)	179, 398, 427, 771, 858, 940, 1019
PleI	GAGTC	2 cut(s)	509, 771
PmaCI	CACGTG	1 cut(s)	733
PmlI	CACGTG	1 cut(s)	733
PpsI	GAGTC	2 cut(s)	509, 771
Ppu21I	YACGTR	2 cut(s)	679, 733
Psp6I	CCWGG	2 cut(s)	471, 1010
PspCI	CACGTG	1 cut(s)	733
PspGI	CCWGG	2 cut(s)	471, 1010
PspLI	CGTACG	1 cut(s)	684
PspN4I	GGNNCC	3 cut(s)	121, 269, 964
PspPI	GGNCC	5 cut(s)	74, 165, 268, 407, 427
PsuI	RGATCY	4 cut(s)	48, 106, 645, 844
RigI	GGCCGGCC	1 cut(s)	510
RsaI	GTAC	7 cut(s)	25, 470, 587, 632, 677, 686, 710
RsaNI	GTAC	7 cut(s)	24, 469, 586, 631, 676, 685, 709
RseI	CAYNNNNRTG	3 cut(s)	435, 735, 923
SaqAI	TTAA	1 cut(s)	477
SatI	GCNGC	7 cut(s)	178, 397, 426, 770, 857, 939, 1018
Sau96I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
SchI	GAGTC	2 cut(s)	509, 771
ScrFI	CCNGG	7 cut(s)	300, 369, 473, 563, 666, 774, 1012
SduI	GDGCHC	3 cut(s)	673, 936, 978
SexAI	ACCWGGT	1 cut(s)	471
SfaNI	GCATC	5 cut(s)	383, 543, 874, 972, 1032
Sfr274I	CTCGAG	1 cut(s)	548
SgrAI	CRCCGGYG	2 cut(s)	41, 859
SinI	GGWCC	1 cut(s)	165
SlaI	CTCGAG	1 cut(s)	548
SmiMI	CAYNNNNRTG	3 cut(s)	435, 735, 923
SmlI	CTYRAG	1 cut(s)	548
SmoI	CTYRAG	1 cut(s)	548
Sse9I	AATT	3 cut(s)	235, 378, 948
SspI	AATATT	1 cut(s)	161
StyD4I	CCNGG	7 cut(s)	298, 367, 471, 561, 664, 772, 1010
StyI	CCWWGG	1 cut(s)	102
TaaI	ACNGT	2 cut(s)	931, 967
TaiI	ACGT	7 cut(s)	100, 297, 363, 390, 447, 681, 735
TasI	AATT	3 cut(s)	235, 378, 948
TatI	WGTACW	1 cut(s)	708
TauI	GCSGC	6 cut(s)	180, 399, 428, 859, 941, 1020
TfiI	GAWTC	3 cut(s)	877, 1029, 1051
Tru1I	TTAA	1 cut(s)	477
Tru9I	TTAA	1 cut(s)	477
TseFI	GTSAC	5 cut(s)	241, 445, 694, 716, 925
TseI	GCWGC	1 cut(s)	769
Tsp45I	GTSAC	5 cut(s)	241, 445, 694, 716, 925
TspGWI	ACGGA	3 cut(s)	716, 806, 858
Van91I	CCANNNNNTGG	1 cut(s)	922
VneI	GTGCAC	1 cut(s)	974
VpaK11BI	GGWCC	1 cut(s)	165
XagI	CCTNNNNNAGG	1 cut(s)	332
XapI	RAATTY	1 cut(s)	378
XceI	RCATGY	1 cut(s)	606
XhoI	CTCGAG	1 cut(s)	548
ZraI	GACGTC	2 cut(s)	361, 388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.