Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0004681

Physical Location & Seq

Genomic Coordinates Forward (+)

1175224 .. 1176291

Gene Structure 1068 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00333810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGGAGTCTCTGACCCTCTTCTGTACTGGTGCTCTCCTTGCCGGCGGGCTCTACTGGTTCGTCTGCGTTCTCGGCCCAGCCGAGCGACAGGGCAAGCGGGCCGCAGATCTCTCCGGCGGCTCCATCTCTGCCGAAAAAGTCCAAGACAGTTACAACCAGTACTGGTCTTTCTTCCGTCGCCCTAAACAGATCGAAGCCTCCGAGAAAGTCCCCGACTTCGTAGACACCTTTTACAACCTTGTCACCGATATTTACGAGTGGGGTTGGGGCCAGTCCTTCCACTTCTCCCCCTCGGTCGCCGGCAAGTCCTACAAGGATGCCACGCGCCTCCACGAGGAGATGGCTGTCGATCTCATTAACGTCGAACCCGGAGCTCGAATCCTAGACGTCGGGTGCGGCGTTGGCGGGCCGATGCGCGCCATTGCAGCCCACTCGAGGGCGAACGTCGTGGGGATCACGATCAACGAGTACCAGGTGAAGAGGGCGCGTCTGCACAACAACAAGGCCGGCCTCGACTCACTCTGCCAAGTTGTGTGCGGCAACTTCCTCCAGATGCCGTTCCCGGAGAACAGCTTCGACGGCGCGTACTCGATCGAGGCGACGTGCCACGCGCCGAAGCTGGAGGAGGTGTACGCCGAGATCTTTAGGGTTCTGAAGCCTGGAGCGCTGTACGCTTCGTACGAGTGGGTGACGACTGACAAGTACAACGGTGACGATGCGGAACACAGGGAGGTGATTCAGGGGATCGAGAGAGGCGACGCCTTGCCGGGGCTGAGGACCCACGTGGACATCGCCGAGGCGGCGAAGAGGGTAGGGTTTGAAGTGGTGAAGGAGAAGGATCTGGCAAAGCCTCCCAGTGAGCCGTGGTGGACTAGGTTGAAGATGGGCAGGATCGCCTACTGGCGCAACCACATTCTGGTTACCGTGCTCTCCTTCTTAGGAATTGCACCCAAGGGAACAGTGGATGTTCACGAGATGCTTTTTGTCACCGCTGACTATTTGACCAGAGGTGGAGAGACCGGAATCTTCACTCCGATGCATATGATCCTCTGCCGGAAGCCGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006275 GO:0006629 GO:0006694 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008156 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009825 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010051 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0012505 GO:0016049 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0045934 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090329 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko00100 ko01110 map00100 map01110

Pfam Domains

Protein Families

Methyltransf_11 Sterol_MT_C

Protein Analysis

355

Amino Acids

39.62

Weight (kDa)

5.97

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	75 - 281	1.5e-14	Mycolic acid cyclopropane synthetase
Ubie_methyltran	PF01209	109 - 225	9e-09	ubiE/COQ5 methyltransferase family
Methyltransf_23	PF13489	122 - 277	2.1e-09	Methyltransferase domain
Methyltransf_31	PF13847	123 - 228	2.3e-15	Methyltransferase domain
Methyltransf_25	PF13649	127 - 221	2.1e-19	Methyltransferase domain
Methyltransf_11	PF08241	128 - 223	5.5e-23	Methyltransferase domain
Methyltransf_12	PF08242	128 - 223	2.6e-11	Methyltransferase domain
Sterol_MT_C	PF08498	291 - 355	1e-24	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20330 AT1G76090
fragaria_vesca	FvH4_2g37920
malus_domestica	MD08G1007400.v1.1 MD15G1006600.v1.1
prunus_persica	Prupe.1G359200_v2.0.a1
pyrus_communis	pycom08g00600 pycom15g00520
rosa_chinensis	RchiOBHm_Chr4g0386051 RchiOBHm_Chr6g0303691
rosa_laevigata	RLG00000010176 RLG00000011056
rosa_multiflora	Rmu_co8442993.1_g000001 Rmu_sc0000472.1_g000013 Rmu_sc0002413.1_g000008 Rmu_sc0026342.1_g000001 Rmu_ssc0000114.1_g000012
rosa_roxburghii	Rroxscaffold_5G00333810 Rroxscaffold_7G00164480
rosa_rugosa	Rorug03G0306500 Rorug06G0324000
rosa_samantha	Rh4AG010400 Rh4BG007500 Rh4CG011100 Rh4DG008800 Rh6AG438300 Rh6BG464800 Rh6CG450600 Rh6DG437200
rosa_wichuraiana	Rw4G000100 Rw6G037930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	390
AccB7I	CCANNNNNTGG	1 cut(s)	916
AccI	GTMKAC	1 cut(s)	222
AccII	CGCG	5 cut(s)	325, 417, 487, 584, 611
AclWI	GGATC	5 cut(s)	461, 752, 846, 899, 1039
AcuI	CTGAAG	1 cut(s)	674
AcvI	CACGTG	1 cut(s)	784
AcyI	GRCGYC	2 cut(s)	387, 759
AfaI	GTAC	8 cut(s)	25, 161, 470, 587, 632, 671, 680, 704
AfeI	AGCGCT	1 cut(s)	666
AfiI	CCNNNNNNNGG	6 cut(s)	334, 435, 436, 768, 916, 1060
AgsI	TTSAA	2 cut(s)	821, 880
AjiI	CACGTC	1 cut(s)	603
AjnI	CCWGG	2 cut(s)	471, 658
AluBI	AGCT	3 cut(s)	374, 573, 619
AluI	AGCT	3 cut(s)	374, 573, 619
Alw21I	GWGCWC	3 cut(s)	34, 376, 930
Alw26I	GTCTC	2 cut(s)	12, 1010
AlwI	GGATC	5 cut(s)	461, 752, 846, 899, 1039
Ama87I	CYCGRG	1 cut(s)	433
Aor51HI	AGCGCT	1 cut(s)	666
AoxI	GGCC	6 cut(s)	73, 99, 268, 407, 504, 508
ApeKI	GCWGC	1 cut(s)	425
Asp700I	GAANNNNTTC	1 cut(s)	572
AspLEI	GCGC	8 cut(s)	327, 417, 419, 487, 584, 613, 667, 906
AspS9I	GGNCC	5 cut(s)	74, 99, 268, 407, 777
AsuC2I	CCSGG	3 cut(s)	369, 563, 768
AsuHPI	GGTGA	7 cut(s)	235, 487, 700, 722, 745, 838, 979
AvaI	CYCGRG	1 cut(s)	433
AvaII	GGWCC	1 cut(s)	777
BanII	GRGCYC	2 cut(s)	51, 376
BauI	CACGAG	2 cut(s)	332, 971
BbrPI	CACGTG	1 cut(s)	784
Bbv12I	GWGCWC	3 cut(s)	34, 376, 930
BbvCI	CCTCAGC	1 cut(s)	773
BbvI	GCAGC	1 cut(s)	437
BccI	CCATC	3 cut(s)	131, 334, 877
BceAI	ACGGC	3 cut(s)	541, 595, 847
BcgI	CGANNNNNNTGC	2 cut(s)	414, 448
BciT130I	CCWGG	2 cut(s)	473, 660
BcnI	CCSGG	3 cut(s)	369, 563, 768
BcoDI	GTCTC	2 cut(s)	12, 1010
BfaI	CTAG	2 cut(s)	383, 873
BfoI	RGCGCY	1 cut(s)	668
BglI	GCCNNNNNGGC	1 cut(s)	800
BglII	AGATCT	2 cut(s)	106, 639
BisI	GCNGC	6 cut(s)	102, 118, 397, 426, 538, 801
BlsI	GCNGC	6 cut(s)	103, 119, 398, 427, 539, 802
BmcAI	AGTACT	1 cut(s)	161
Bme1390I	CCNGG	5 cut(s)	369, 473, 563, 660, 768
Bme18I	GGWCC	1 cut(s)	777
BmeT110I	CYCGRG	1 cut(s)	433
BmgBI	CACGTC	1 cut(s)	603
BmgT120I	GGNCC	5 cut(s)	74, 99, 268, 407, 777
BmiI	GGNNCC	3 cut(s)	121, 269, 779
BmrFI	CCNGG	5 cut(s)	369, 473, 563, 660, 768
BmrI	ACTGGG	1 cut(s)	849
BmsI	GCATC	6 cut(s)	307, 402, 543, 706, 966, 1026
BmuI	ACTGGG	1 cut(s)	849
BpmI	CTGGAG	3 cut(s)	533, 641, 681
Bpu10I	CCTNAGC	1 cut(s)	773
BpuMI	CCSGG	3 cut(s)	369, 563, 768
BsaAI	YACGTR	1 cut(s)	784
BsaBI	GATNNNNATC	1 cut(s)	458
BsaHI	GRCGYC	2 cut(s)	387, 759
BsaI	GGTCTC	1 cut(s)	1010
BsaJI	CCNNGG	5 cut(s)	291, 767, 795, 863, 951
BsaWI	WCCGGW	1 cut(s)	1019
BsaXI	ACNNNNNCTCC	2 cut(s)	614, 644
Bsc4I	CCNNNNNNNGG	6 cut(s)	334, 435, 436, 768, 916, 1060
Bse118I	RCCGGY	3 cut(s)	41, 299, 506
Bse1I	ACTGG	7 cut(s)	31, 59, 157, 167, 271, 855, 905
Bse3DI	GCAATG	1 cut(s)	420
Bse8I	GATNNNNATC	1 cut(s)	458
BseBI	CCWGG	2 cut(s)	473, 660
BseDI	CCNNGG	5 cut(s)	291, 767, 795, 863, 951
BseGI	GGATG	2 cut(s)	322, 970
BseJI	GATNNNNATC	1 cut(s)	458
BseLI	CCNNNNNNNGG	6 cut(s)	334, 435, 436, 768, 916, 1060
BseMI	GCAATG	1 cut(s)	420
BseMII	CTCAG	1 cut(s)	764
BseNI	ACTGG	7 cut(s)	31, 59, 157, 167, 271, 855, 905
BsePI	GCGCGC	1 cut(s)	415
BseRI	GAGGAG	2 cut(s)	350, 638
BseXI	GCAGC	1 cut(s)	437
BseYI	CCCAGC	1 cut(s)	76
BsgI	GTGCAG	1 cut(s)	476
Bsh1236I	CGCG	5 cut(s)	325, 417, 487, 584, 611
Bsh1285I	CGRYCG	2 cut(s)	297, 594
BshFI	GGCC	6 cut(s)	75, 101, 270, 409, 506, 510
BsiEI	CGRYCG	2 cut(s)	297, 594
BsiHKAI	GWGCWC	3 cut(s)	34, 376, 930
BsiHKCI	CYCGRG	1 cut(s)	433
BsiWI	CGTACG	1 cut(s)	678
BslFI	GGGAC	1 cut(s)	194
BslI	CCNNNNNNNGG	6 cut(s)	334, 435, 436, 768, 916, 1060
BsmAI	GTCTC	2 cut(s)	12, 1010
BsmFI	GGGAC	1 cut(s)	194
BsnI	GGCC	6 cut(s)	75, 101, 270, 409, 506, 510
Bso31I	GGTCTC	1 cut(s)	1010
BsoBI	CYCGRG	1 cut(s)	433
Bsp1286I	GDGCHC	4 cut(s)	34, 51, 376, 930
BspANI	GGCC	6 cut(s)	75, 101, 270, 409, 506, 510
BspCNI	CTCAG	1 cut(s)	765
BspFNI	CGCG	5 cut(s)	325, 417, 487, 584, 611
BspLI	GGNNCC	3 cut(s)	121, 269, 779
BspPI	GGATC	5 cut(s)	461, 752, 846, 899, 1039
BspTNI	GGTCTC	1 cut(s)	1010
BsrDI	GCAATG	1 cut(s)	420
BsrFI	RCCGGY	3 cut(s)	41, 299, 506
BsrI	ACTGG	7 cut(s)	31, 59, 157, 167, 271, 855, 905
BssAI	RCCGGY	3 cut(s)	41, 299, 506
BssECI	CCNNGG	5 cut(s)	291, 767, 795, 863, 951
BssHII	GCGCGC	1 cut(s)	415
BssNI	GRCGYC	2 cut(s)	387, 759
BssSI	CACGAG	2 cut(s)	332, 971
BssT1I	CCWWGG	1 cut(s)	951
Bst2BI	CACGAG	2 cut(s)	332, 971
Bst2UI	CCWGG	2 cut(s)	473, 660
Bst4CI	ACNGT	4 cut(s)	149, 710, 925, 961
Bst6I	CTCTTC	3 cut(s)	23, 473, 800
BstACI	GRCGYC	2 cut(s)	387, 759
BstBAI	YACGTR	1 cut(s)	784
BstC8I	GCNNGC	8 cut(s)	43, 47, 95, 99, 301, 407, 417, 508
BstDEI	CTNAG	2 cut(s)	773, 937
BstDSI	CCRYGG	1 cut(s)	863
BstEII	GGTNACC	1 cut(s)	919
BstENI	CCTNNNNNAGG	1 cut(s)	332
BstF5I	GGATG	2 cut(s)	322, 970
BstFNI	CGCG	5 cut(s)	325, 417, 487, 584, 611
BstH2I	RGCGCY	1 cut(s)	668
BstHHI	GCGC	8 cut(s)	327, 417, 419, 487, 584, 613, 667, 906
BstMAI	GTCTC	2 cut(s)	12, 1010
BstMCI	CGRYCG	2 cut(s)	297, 594
BstMWI	GCNNNNNNNGC	8 cut(s)	38, 72, 402, 425, 579, 664, 671, 800
BstNI	CCWGG	2 cut(s)	473, 660
BstPI	GGTNACC	1 cut(s)	919
BstSCI	CCNGG	5 cut(s)	367, 471, 561, 658, 766
BstUI	CGCG	5 cut(s)	325, 417, 487, 584, 611
BstV1I	GCAGC	1 cut(s)	437
BstX2I	RGATCY	3 cut(s)	106, 639, 838
BstYI	RGATCY	3 cut(s)	106, 639, 838
BsuRI	GGCC	6 cut(s)	75, 101, 270, 409, 506, 510
BtgI	CCRYGG	1 cut(s)	863
BtgZI	GCGATG	1 cut(s)	775
BtrI	CACGTC	1 cut(s)	603
BtsCI	GGATG	2 cut(s)	322, 970
BtsIMutI	CAGTG	2 cut(s)	862, 966
Cac8I	GCNNGC	8 cut(s)	43, 47, 95, 99, 301, 407, 417, 508
CfoI	GCGC	8 cut(s)	327, 417, 419, 487, 584, 613, 667, 906
Cfr10I	RCCGGY	3 cut(s)	41, 299, 506
Cfr13I	GGNCC	5 cut(s)	74, 99, 268, 407, 777
CseI	GACGC	2 cut(s)	476, 767
CsiI	ACCWGGT	1 cut(s)	471
Csp6I	GTAC	8 cut(s)	24, 160, 469, 586, 631, 670, 679, 703
CviQI	GTAC	8 cut(s)	24, 160, 469, 586, 631, 670, 679, 703
DdeI	CTNAG	2 cut(s)	773, 937
Eam1104I	CTCTTC	3 cut(s)	23, 473, 800
EarI	CTCTTC	3 cut(s)	23, 473, 800
Ecl136II	GAGCTC	1 cut(s)	374
Eco130I	CCWWGG	1 cut(s)	951
Eco24I	GRGCYC	2 cut(s)	51, 376
Eco31I	GGTCTC	1 cut(s)	1010
Eco47I	GGWCC	1 cut(s)	777
Eco47III	AGCGCT	1 cut(s)	666
Eco53kI	GAGCTC	1 cut(s)	374
Eco57I	CTGAAG	1 cut(s)	674
Eco72I	CACGTG	1 cut(s)	784
Eco88I	CYCGRG	1 cut(s)	433
Eco91I	GGTNACC	1 cut(s)	919
EcoICRI	GAGCTC	1 cut(s)	374
EcoNI	CCTNNNNNAGG	1 cut(s)	332
EcoO109I	RGGNCCY	1 cut(s)	777
EcoO65I	GGTNACC	1 cut(s)	919
EcoRII	CCWGG	2 cut(s)	471, 658
EcoT14I	CCWWGG	1 cut(s)	951
EcoT22I	ATGCAT	1 cut(s)	1041
EcoT38I	GRGCYC	2 cut(s)	51, 376
ErhI	CCWWGG	1 cut(s)	951
FaiI	YATR	2 cut(s)	1041, 1043
FaqI	GGGAC	1 cut(s)	194
FauI	CCCGC	3 cut(s)	38, 90, 398
FauNDI	CATATG	1 cut(s)	1041
FblI	GTMKAC	1 cut(s)	222
Fnu4HI	GCNGC	6 cut(s)	102, 118, 397, 426, 538, 801
FokI	GGATG	2 cut(s)	329, 977
FriOI	GRGCYC	2 cut(s)	51, 376
FseI	GGCCGGCC	1 cut(s)	510
Fsp4HI	GCNGC	6 cut(s)	102, 118, 397, 426, 538, 801
FspBI	CTAG	2 cut(s)	383, 873
GlaI	GCGC	8 cut(s)	326, 416, 418, 486, 583, 612, 666, 905
GluI	GCNGC	6 cut(s)	102, 118, 397, 426, 538, 801
GsaI	CCCAGC	1 cut(s)	80
GsuI	CTGGAG	3 cut(s)	533, 641, 681
HaeII	RGCGCY	1 cut(s)	668
HaeIII	GGCC	6 cut(s)	75, 101, 270, 409, 506, 510
HgaI	GACGC	2 cut(s)	476, 767
HhaI	GCGC	8 cut(s)	327, 417, 419, 487, 584, 613, 667, 906
Hin1I	GRCGYC	2 cut(s)	387, 759
Hin6I	GCGC	8 cut(s)	325, 415, 417, 485, 582, 611, 665, 904
HinP1I	GCGC	8 cut(s)	325, 415, 417, 485, 582, 611, 665, 904
HinfI	GANTC	5 cut(s)	5, 378, 515, 736, 1023
HphI	GGTGA	7 cut(s)	235, 487, 700, 722, 745, 838, 979
Hpy166II	GTNNAC	5 cut(s)	223, 631, 787, 870, 970
Hpy188I	TCNGA	4 cut(s)	12, 202, 654, 1035
Hpy188III	TCNNGA	4 cut(s)	457, 550, 748, 971
Hpy8I	GTNNAC	5 cut(s)	223, 631, 787, 870, 970
Hpy99I	CGWCG	7 cut(s)	180, 365, 392, 449, 581, 604, 761
HpyAV	CCTTC	4 cut(s)	286, 823, 829, 943
HpyCH4III	ACNGT	4 cut(s)	149, 710, 925, 961
HpyCH4IV	ACGT	5 cut(s)	360, 387, 444, 602, 783
HpyCH4V	TGCA	4 cut(s)	425, 493, 947, 1039
HpyF10VI	GCNNNNNNNGC	8 cut(s)	38, 72, 402, 425, 579, 664, 671, 800
HpyF3I	CTNAG	2 cut(s)	773, 937
HpySE526I	ACGT	5 cut(s)	360, 387, 444, 602, 783
Hsp92I	GRCGYC	2 cut(s)	387, 759
HspAI	GCGC	8 cut(s)	325, 415, 417, 485, 582, 611, 665, 904
KroI	GCCGGC	3 cut(s)	41, 299, 506
KroNI	GCCGGC	3 cut(s)	43, 301, 508
LmnI	GCTCC	3 cut(s)	125, 371, 662
Lsp1109I	GCAGC	1 cut(s)	437
LweI	GCATC	6 cut(s)	307, 402, 543, 706, 966, 1026
MabI	ACCWGGT	1 cut(s)	471
MaeI	CTAG	2 cut(s)	383, 873
MaeII	ACGT	5 cut(s)	360, 387, 444, 602, 783
MaeIII	GTNAC	6 cut(s)	149, 241, 688, 710, 919, 985
MboII	GAAGA	6 cut(s)	10, 163, 490, 817, 892, 1018
MflI	RGATCY	3 cut(s)	106, 639, 838
MhlI	GDGCHC	4 cut(s)	34, 51, 376, 930
MluCI	AATT	1 cut(s)	942
MlyI	GAGTC	2 cut(s)	14, 509
Mph1103I	ATGCAT	1 cut(s)	1041
MroNI	GCCGGC	3 cut(s)	41, 299, 506
MroXI	GAANNNNTTC	1 cut(s)	572
MseI	TTAA	1 cut(s)	357
MslI	CAYNNNNRTG	1 cut(s)	1034
MspA1I	CMGCKG	1 cut(s)	992
MspR9I	CCNGG	5 cut(s)	369, 473, 563, 660, 768
MvaI	CCWGG	2 cut(s)	473, 660
MvnI	CGCG	5 cut(s)	325, 417, 487, 584, 611
MwoI	GCNNNNNNNGC	8 cut(s)	38, 72, 402, 425, 579, 664, 671, 800
NaeI	GCCGGC	3 cut(s)	43, 301, 508
NciI	CCSGG	3 cut(s)	369, 563, 768
NdeI	CATATG	1 cut(s)	1041
NgoMIV	GCCGGC	3 cut(s)	41, 299, 506
NlaIV	GGNNCC	3 cut(s)	121, 269, 779
NmeAIII	GCCGAG	4 cut(s)	51, 106, 661, 820
NmuCI	GTSAC	4 cut(s)	241, 688, 710, 985
NsiI	ATGCAT	1 cut(s)	1041
PaeR7I	CTCGAG	1 cut(s)	433
PauI	GCGCGC	1 cut(s)	415
PcsI	WCGNNNNNNNCGW	6 cut(s)	78, 198, 396, 596, 678, 753
PdiI	GCCGGC	3 cut(s)	43, 301, 508
PdmI	GAANNNNTTC	1 cut(s)	572
PfeI	GAWTC	3 cut(s)	378, 736, 1023
Pfl23II	CGTACG	1 cut(s)	678
PflMI	CCANNNNNTGG	1 cut(s)	916
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	6 cut(s)	103, 119, 398, 427, 539, 802
Ple19I	CGATCG	1 cut(s)	594
PleI	GAGTC	2 cut(s)	13, 509
PmaCI	CACGTG	1 cut(s)	784
PmlI	CACGTG	1 cut(s)	784
PpsI	GAGTC	2 cut(s)	13, 509
Ppu21I	YACGTR	1 cut(s)	784
PpuMI	RGGWCCY	1 cut(s)	777
Psp124BI	GAGCTC	1 cut(s)	376
Psp5II	RGGWCCY	1 cut(s)	777
Psp6I	CCWGG	2 cut(s)	471, 658
PspCI	CACGTG	1 cut(s)	784
PspEI	GGTNACC	1 cut(s)	919
PspFI	CCCAGC	1 cut(s)	76
PspGI	CCWGG	2 cut(s)	471, 658
PspLI	CGTACG	1 cut(s)	678
PspN4I	GGNNCC	3 cut(s)	121, 269, 779
PspPI	GGNCC	5 cut(s)	74, 99, 268, 407, 777
PspPPI	RGGWCCY	1 cut(s)	777
PspXI	VCTCGAGB	1 cut(s)	433
PsuI	RGATCY	3 cut(s)	106, 639, 838
PteI	GCGCGC	1 cut(s)	415
PvuI	CGATCG	1 cut(s)	594
RigI	GGCCGGCC	1 cut(s)	510
RsaI	GTAC	8 cut(s)	25, 161, 470, 587, 632, 671, 680, 704
RsaNI	GTAC	8 cut(s)	24, 160, 469, 586, 631, 670, 679, 703
RseI	CAYNNNNRTG	1 cut(s)	1034
SacI	GAGCTC	1 cut(s)	376
SaqAI	TTAA	1 cut(s)	357
SatI	GCNGC	6 cut(s)	102, 118, 397, 426, 538, 801
Sau96I	GGNCC	5 cut(s)	74, 99, 268, 407, 777
ScaI	AGTACT	1 cut(s)	161
SchI	GAGTC	2 cut(s)	14, 509
ScrFI	CCNGG	5 cut(s)	369, 473, 563, 660, 768
SduI	GDGCHC	4 cut(s)	34, 51, 376, 930
SexAI	ACCWGGT	1 cut(s)	471
SfaNI	GCATC	6 cut(s)	307, 402, 543, 706, 966, 1026
Sfr274I	CTCGAG	1 cut(s)	433
SinI	GGWCC	1 cut(s)	777
SlaI	CTCGAG	1 cut(s)	433
SmiMI	CAYNNNNRTG	1 cut(s)	1034
SmlI	CTYRAG	1 cut(s)	433
SmoI	CTYRAG	1 cut(s)	433
Sse9I	AATT	1 cut(s)	942
SspMI	CTAG	2 cut(s)	383, 873
SstI	GAGCTC	1 cut(s)	376
StyD4I	CCNGG	5 cut(s)	367, 471, 561, 658, 766
StyI	CCWWGG	1 cut(s)	951
TaaI	ACNGT	4 cut(s)	149, 710, 925, 961
TaiI	ACGT	5 cut(s)	363, 390, 447, 605, 786
TaqII	GACCGA	1 cut(s)	283
TasI	AATT	1 cut(s)	942
TatI	WGTACW	3 cut(s)	23, 159, 702
TauI	GCSGC	5 cut(s)	104, 120, 399, 540, 803
TfiI	GAWTC	3 cut(s)	378, 736, 1023
Tru1I	TTAA	1 cut(s)	357
Tru9I	TTAA	1 cut(s)	357
TscAI	CASTG	2 cut(s)	862, 966
TseFI	GTSAC	4 cut(s)	241, 688, 710, 985
TseI	GCWGC	1 cut(s)	425
Tsp45I	GTSAC	4 cut(s)	241, 688, 710, 985
TspGWI	ACGGA	1 cut(s)	164
TspRI	CASTG	2 cut(s)	862, 966
Van91I	CCANNNNNTGG	1 cut(s)	916
VpaK11BI	GGWCC	1 cut(s)	777
XagI	CCTNNNNNAGG	1 cut(s)	332
XcmI	CCANNNNNNNNNTGG	1 cut(s)	958
XhoI	CTCGAG	1 cut(s)	433
XmiI	GTMKAC	1 cut(s)	222
XmnI	GAANNNNTTC	1 cut(s)	572
XspI	CTAG	2 cut(s)	383, 873
ZraI	GACGTC	1 cut(s)	388
ZrmI	AGTACT	1 cut(s)	161
Zsp2I	ATGCAT	1 cut(s)	1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.