Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004681

Physical Location & Seq

Genomic Coordinates Forward (+)

580729 .. 582069

Gene Structure 1341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1007400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGACTCTCTCACCCTCTTTTGTACGGGCGCTCTCCTCGCCGGCGGGATCTATTGGTTTGTCTGCATTCTCGGCCCCGCCGAGCGAAAGGGGAAGCGCGCCGTAGATCTCTCCGGTGGCTCCATCTCCGCCGAGAAAGTTCAAGATAGCTACGACAAATATTGGTCCTTCTTCCGCCGCCCAAAAGAGATCGAAACGGCTGAGAAAGTCCCCGATTTTGTAGACACCTTTTACAACTTAGTCACCGATATTTATGAGTGGGGTTGGGGCCAGTCCTTCCACTTCTCCCCGCACATCCCCGGTAAGTCCCACAAAGACGCCACGCGCCTCCACGAGGAGATGGCCGTCGATCTCATCGATGTAAAACCCGGAGACCGAATTCTTGACGTCGGATGCGGCGTGGGTGGCCCTATGCGTGCCATCGCGGCCCACTCGCGGGCCAACGTCACAGGGATCACGATCAACGAGTACCAGGTGAAACGCGCGCGTCTGCACAACAAGAAGGCCGGCCTCGACTCGCTCTGCGAGGTCGTATGCGGGAACTTCCTCGAGATGCCGTTCCCGGATAACAGCTTCGACGGCGCGTACTCTATCGAGGCTACATGTCACGCGCCGAAGCTGGAGGAGGTGTACGCCGAGATCTTCCGGGTTTTGAAGCCCGGTGCTCTGTACGTGTCGTACGAGTGGGTGACGACAGACAAGTACAAGGGTGAGGACGCTGAACACGTGGAGGTCATTCAGGGAATCGAGAGAGGGGACGCCCTGCCGGGACTTAGGAGCTATACGGACATTGCAGAGACGGCGAGGAAGGTCGGTTTCGAGGTGGTGAAGGAGAACGATCTGGCCAAGCCGCCGGCGCAGCCGTGGTGGAGTCGATTGAAGATGGGGAGGATCGCCTACTGGCGCAACCACATTCTGGTGACGGTGCTCGCCGCCGTCGGAATTGCACCCAAGGGAACCGTGGATGTGCACGAGATGCTGTTCAAAACTGCCGACTTTTTGACCAGGGGCGGCGAAACCGGAATCTTTAGTCCGATGCACATGATTCTCTGCCGGAAGCCCGAAACACCCAAATCATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006275 GO:0006629 GO:0006694 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008156 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009825 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010051 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0012505 GO:0016049 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0045934 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090329 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko00100 ko01110 map00100 map01110

Pfam Domains

Protein Families

Methyltransf_11 Sterol_MT_C

Protein Analysis

361

Amino Acids

40.17

Weight (kDa)

6.15

Isoelectric Point (pI)

34.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	77 - 281	4.2e-15	Mycolic acid cyclopropane synthetase
Ubie_methyltran	PF01209	110 - 228	4.7e-10	ubiE/COQ5 methyltransferase family
Methyltransf_31	PF13847	122 - 229	9.8e-17	Methyltransferase domain
Methyltransf_23	PF13489	122 - 277	1.3e-09	Methyltransferase domain
Methyltransf_25	PF13649	127 - 221	3.3e-20	Methyltransferase domain
Methyltransf_11	PF08241	128 - 224	8e-24	Methyltransferase domain
Methyltransf_12	PF08242	128 - 223	1.5e-11	Methyltransferase domain
Sterol_MT_C	PF08498	291 - 355	7.8e-23	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20330 AT1G76090
fragaria_vesca	FvH4_2g37920
malus_domestica	MD08G1007400.v1.1 MD15G1006600.v1.1
prunus_persica	Prupe.1G359200_v2.0.a1
pyrus_communis	pycom08g00600 pycom15g00520
rosa_chinensis	RchiOBHm_Chr4g0386051 RchiOBHm_Chr6g0303691
rosa_laevigata	RLG00000010176 RLG00000011056
rosa_multiflora	Rmu_co8442993.1_g000001 Rmu_sc0000472.1_g000013 Rmu_sc0002413.1_g000008 Rmu_sc0026342.1_g000001 Rmu_ssc0000114.1_g000012
rosa_roxburghii	Rroxscaffold_5G00333810 Rroxscaffold_7G00164480
rosa_rugosa	Rorug03G0306500 Rorug06G0324000
rosa_samantha	Rh4AG010400 Rh4BG007500 Rh4CG011100 Rh4DG008800 Rh6AG438300 Rh6BG464800 Rh6CG450600 Rh6DG437200
rosa_wichuraiana	Rw4G000100 Rw6G037930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	390
AccB7I	CCANNNNNTGG	1 cut(s)	916
AccI	GTMKAC	1 cut(s)	222
AccII	CGCG	9 cut(s)	99, 325, 425, 436, 483, 485, 487, 584, 611
AclWI	GGATC	3 cut(s)	56, 461, 899
AcoI	YGGCCR	2 cut(s)	342, 843
AcsI	RAATTY	1 cut(s)	378
AcvI	CACGTG	1 cut(s)	727
AcyI	GRCGYC	3 cut(s)	318, 387, 759
AfaI	GTAC	7 cut(s)	25, 470, 587, 632, 671, 680, 704
AfiI	CCNNNNNNNGG	4 cut(s)	334, 435, 436, 916
AflIII	ACRYGT	3 cut(s)	602, 672, 724
AgsI	TTSAA	4 cut(s)	143, 655, 880, 985
AjnI	CCWGG	2 cut(s)	471, 1004
AluBI	AGCT	4 cut(s)	150, 573, 619, 780
AluI	AGCT	4 cut(s)	150, 573, 619, 780
Alw21I	GWGCWC	3 cut(s)	667, 930, 972
Alw26I	GTCTC	2 cut(s)	366, 791
Alw44I	GTGCAC	1 cut(s)	968
AlwI	GGATC	3 cut(s)	56, 461, 899
Ama87I	CYCGRG	1 cut(s)	548
AoxI	GGCC	9 cut(s)	73, 268, 342, 406, 426, 438, 504, 508, 843
ApaLI	GTGCAC	1 cut(s)	968
ApeKI	GCWGC	1 cut(s)	859
ApoI	RAATTY	1 cut(s)	378
AspS9I	GGNCC	6 cut(s)	74, 165, 268, 407, 427, 438
AsuC2I	CCSGG	6 cut(s)	300, 369, 563, 647, 660, 768
AsuHPI	GGTGA	7 cut(s)	4, 235, 487, 700, 722, 838, 931
AvaI	CYCGRG	1 cut(s)	548
AvaII	GGWCC	1 cut(s)	165
BaeGI	GKGCMC	1 cut(s)	972
BalI	TGGCCA	1 cut(s)	845
BauI	CACGAG	2 cut(s)	332, 971
BbrPI	CACGTG	1 cut(s)	727
Bbv12I	GWGCWC	3 cut(s)	667, 930, 972
BbvI	GCAGC	1 cut(s)	871
BccI	CCATC	4 cut(s)	131, 334, 428, 877
BceAI	ACGGC	8 cut(s)	86, 213, 329, 541, 595, 816, 847, 920
BciT130I	CCWGG	2 cut(s)	473, 1006
BcnI	CCSGG	6 cut(s)	300, 369, 563, 647, 660, 768
BcoDI	GTCTC	2 cut(s)	366, 791
BfoI	RGCGCY	1 cut(s)	33
BglI	GCCNNNNNGGC	1 cut(s)	425
BglII	AGATCT	2 cut(s)	106, 639
BisI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
BlsI	GCNGC	7 cut(s)	179, 398, 427, 852, 861, 934, 1013
Bme1390I	CCNGG	8 cut(s)	300, 369, 473, 563, 647, 660, 768, 1006
Bme18I	GGWCC	1 cut(s)	165
BmeT110I	CYCGRG	1 cut(s)	548
BmgT120I	GGNCC	6 cut(s)	74, 165, 268, 407, 427, 438
BmiI	GGNNCC	4 cut(s)	76, 121, 269, 958
BmrFI	CCNGG	8 cut(s)	300, 369, 473, 563, 647, 660, 768, 1006
BmsI	GCATC	4 cut(s)	383, 543, 966, 1026
BpmI	CTGGAG	1 cut(s)	641
BpuMI	CCSGG	6 cut(s)	300, 369, 563, 647, 660, 768
Bsa29I	ATCGAT	1 cut(s)	357
BsaAI	YACGTR	2 cut(s)	673, 727
BsaBI	GATNNNNATC	1 cut(s)	458
BsaHI	GRCGYC	3 cut(s)	318, 387, 759
BsaI	GGTCTC	1 cut(s)	366
BsaJI	CCNNGG	5 cut(s)	298, 863, 951, 960, 1005
BsaWI	WCCGGW	2 cut(s)	113, 1019
BsaXI	ACNNNNNCTCC	2 cut(s)	614, 644
Bsc4I	CCNNNNNNNGG	4 cut(s)	334, 435, 436, 916
Bse118I	RCCGGY	3 cut(s)	41, 506, 853
Bse1I	ACTGG	2 cut(s)	271, 905
Bse3DI	GCAATG	1 cut(s)	789
Bse8I	GATNNNNATC	1 cut(s)	458
BseBI	CCWGG	2 cut(s)	473, 1006
BseCI	ATCGAT	1 cut(s)	357
BseDI	CCNNGG	5 cut(s)	298, 863, 951, 960, 1005
BseGI	GGATG	3 cut(s)	294, 398, 970
BseJI	GATNNNNATC	1 cut(s)	458
BseLI	CCNNNNNNNGG	4 cut(s)	334, 435, 436, 916
BseMI	GCAATG	1 cut(s)	789
BseMII	CTCAG	1 cut(s)	192
BseNI	ACTGG	2 cut(s)	271, 905
BsePI	GCGCGC	2 cut(s)	97, 483
BseRI	GAGGAG	3 cut(s)	26, 350, 638
BseSI	GKGCMC	1 cut(s)	972
BseXI	GCAGC	1 cut(s)	871
BsgI	GTGCAG	1 cut(s)	476
Bsh1236I	CGCG	9 cut(s)	99, 325, 425, 436, 483, 485, 487, 584, 611
BshFI	GGCC	9 cut(s)	75, 270, 344, 408, 428, 440, 506, 510, 845
BshVI	ATCGAT	1 cut(s)	357
BsiHKAI	GWGCWC	3 cut(s)	667, 930, 972
BsiHKCI	CYCGRG	1 cut(s)	548
BsiWI	CGTACG	1 cut(s)	678
BslFI	GGGAC	4 cut(s)	194, 292, 770, 783
BslI	CCNNNNNNNGG	4 cut(s)	334, 435, 436, 916
BsmAI	GTCTC	2 cut(s)	366, 791
BsmBI	CGTCTC	1 cut(s)	791
BsmFI	GGGAC	4 cut(s)	194, 292, 770, 783
BsmI	GAATGC	1 cut(s)	66
BsnI	GGCC	9 cut(s)	75, 270, 344, 408, 428, 440, 506, 510, 845
Bso31I	GGTCTC	1 cut(s)	366
BsoBI	CYCGRG	1 cut(s)	548
Bsp1286I	GDGCHC	3 cut(s)	667, 930, 972
Bsp143I	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
BspANI	GGCC	9 cut(s)	75, 270, 344, 408, 428, 440, 506, 510, 845
BspCNI	CTCAG	1 cut(s)	193
BspDI	ATCGAT	1 cut(s)	357
BspFNI	CGCG	9 cut(s)	99, 325, 425, 436, 483, 485, 487, 584, 611
BspLI	GGNNCC	4 cut(s)	76, 121, 269, 958
BspPI	GGATC	3 cut(s)	56, 461, 899
BspTNI	GGTCTC	1 cut(s)	366
BsrDI	GCAATG	1 cut(s)	789
BsrFI	RCCGGY	3 cut(s)	41, 506, 853
BsrI	ACTGG	2 cut(s)	271, 905
BssAI	RCCGGY	3 cut(s)	41, 506, 853
BssECI	CCNNGG	5 cut(s)	298, 863, 951, 960, 1005
BssHII	GCGCGC	2 cut(s)	97, 483
BssMI	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
BssNI	GRCGYC	3 cut(s)	318, 387, 759
BssSI	CACGAG	2 cut(s)	332, 971
BssT1I	CCWWGG	1 cut(s)	951
Bst2BI	CACGAG	2 cut(s)	332, 971
Bst2UI	CCWGG	2 cut(s)	473, 1006
Bst4CI	ACNGT	2 cut(s)	925, 961
BstACI	GRCGYC	3 cut(s)	318, 387, 759
BstAPI	GCANNNNNTGC	1 cut(s)	976
BstBAI	YACGTR	2 cut(s)	673, 727
BstC8I	GCNNGC	8 cut(s)	43, 99, 417, 438, 485, 508, 855, 930
BstDEI	CTNAG	3 cut(s)	201, 238, 773
BstDSI	CCRYGG	2 cut(s)	863, 960
BstENI	CCTNNNNNAGG	1 cut(s)	332
BstF5I	GGATG	3 cut(s)	294, 398, 970
BstFNI	CGCG	9 cut(s)	99, 325, 425, 436, 483, 485, 487, 584, 611
BstH2I	RGCGCY	1 cut(s)	33
BstKTI	GATC	9 cut(s)	51, 109, 192, 352, 456, 462, 642, 841, 894
BstMAI	GTCTC	2 cut(s)	366, 791
BstMBI	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
BstMWI	GCNNNNNNNGC	9 cut(s)	38, 72, 405, 425, 579, 800, 856, 859, 976
BstNI	CCWGG	2 cut(s)	473, 1006
BstNSI	RCATGY	1 cut(s)	606
BstSCI	CCNGG	8 cut(s)	298, 367, 471, 561, 645, 658, 766, 1004
BstSLI	GKGCMC	1 cut(s)	972
BstUI	CGCG	9 cut(s)	99, 325, 425, 436, 483, 485, 487, 584, 611
BstV1I	GCAGC	1 cut(s)	871
BstX2I	RGATCY	3 cut(s)	48, 106, 639
BstYI	RGATCY	3 cut(s)	48, 106, 639
Bsu15I	ATCGAT	1 cut(s)	357
BsuRI	GGCC	9 cut(s)	75, 270, 344, 408, 428, 440, 506, 510, 845
BsuTUI	ATCGAT	1 cut(s)	357
BtgI	CCRYGG	2 cut(s)	863, 960
BtgZI	GCGATG	1 cut(s)	406
BtsCI	GGATG	3 cut(s)	294, 398, 970
Cac8I	GCNNGC	8 cut(s)	43, 99, 417, 438, 485, 508, 855, 930
Cfr10I	RCCGGY	3 cut(s)	41, 506, 853
Cfr13I	GGNCC	6 cut(s)	74, 165, 268, 407, 427, 438
ClaI	ATCGAT	1 cut(s)	357
CseI	GACGC	4 cut(s)	326, 476, 725, 767
CsiI	ACCWGGT	1 cut(s)	471
Csp6I	GTAC	7 cut(s)	24, 469, 586, 631, 670, 679, 703
CviAII	CATG	2 cut(s)	603, 1042
CviQI	GTAC	7 cut(s)	24, 469, 586, 631, 670, 679, 703
DdeI	CTNAG	3 cut(s)	201, 238, 773
DpnI	GATC	9 cut(s)	50, 108, 191, 351, 455, 461, 641, 840, 893
DpnII	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
EaeI	YGGCCR	2 cut(s)	342, 843
EciI	GGCGGA	2 cut(s)	118, 164
Eco130I	CCWWGG	1 cut(s)	951
Eco31I	GGTCTC	1 cut(s)	366
Eco47I	GGWCC	1 cut(s)	165
Eco72I	CACGTG	1 cut(s)	727
Eco88I	CYCGRG	1 cut(s)	548
EcoNI	CCTNNNNNAGG	1 cut(s)	332
EcoRI	GAATTC	1 cut(s)	378
EcoRII	CCWGG	2 cut(s)	471, 1004
EcoT14I	CCWWGG	1 cut(s)	951
ErhI	CCWWGG	1 cut(s)	951
Esp3I	CGTCTC	1 cut(s)	791
FaeI	CATG	2 cut(s)	606, 1045
FaiI	YATR	6 cut(s)	255, 413, 535, 604, 783, 1043
FaqI	GGGAC	4 cut(s)	194, 292, 770, 783
FatI	CATG	2 cut(s)	602, 1041
FauI	CCCGC	5 cut(s)	38, 85, 297, 429, 530
FblI	GTMKAC	1 cut(s)	222
Fnu4HI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
FokI	GGATG	3 cut(s)	281, 405, 977
FseI	GGCCGGCC	1 cut(s)	510
Fsp4HI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
GluI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
GsuI	CTGGAG	1 cut(s)	641
HaeII	RGCGCY	1 cut(s)	33
HaeIII	GGCC	9 cut(s)	75, 270, 344, 408, 428, 440, 506, 510, 845
HgaI	GACGC	4 cut(s)	326, 476, 725, 767
Hin1I	GRCGYC	3 cut(s)	318, 387, 759
Hin1II	CATG	2 cut(s)	606, 1045
HinfI	GANTC	6 cut(s)	5, 515, 744, 871, 1023, 1045
HphI	GGTGA	7 cut(s)	4, 235, 487, 700, 722, 838, 931
Hpy166II	GTNNAC	3 cut(s)	223, 631, 970
Hpy188I	TCNGA	3 cut(s)	392, 941, 1035
Hpy188III	TCNNGA	5 cut(s)	143, 383, 457, 550, 748
Hpy8I	GTNNAC	3 cut(s)	223, 631, 970
Hpy99I	CGWCG	4 cut(s)	350, 392, 581, 941
HpyAV	CCTTC	5 cut(s)	178, 286, 496, 802, 823
HpyCH4III	ACNGT	2 cut(s)	925, 961
HpyCH4IV	ACGT	4 cut(s)	387, 444, 672, 726
HpyCH4V	TGCA	6 cut(s)	66, 493, 794, 947, 970, 1039
HpyF10VI	GCNNNNNNNGC	9 cut(s)	38, 72, 405, 425, 579, 800, 856, 859, 976
HpyF3I	CTNAG	3 cut(s)	201, 238, 773
HpySE526I	ACGT	4 cut(s)	387, 444, 672, 726
Hsp92I	GRCGYC	3 cut(s)	318, 387, 759
Hsp92II	CATG	2 cut(s)	606, 1045
KroI	GCCGGC	3 cut(s)	41, 506, 853
KroNI	GCCGGC	3 cut(s)	43, 508, 855
Kzo9I	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
LmnI	GCTCC	2 cut(s)	125, 777
Lsp1109I	GCAGC	1 cut(s)	871
LweI	GCATC	4 cut(s)	383, 543, 966, 1026
MabI	ACCWGGT	1 cut(s)	471
MaeII	ACGT	4 cut(s)	387, 444, 672, 726
MaeIII	GTNAC	5 cut(s)	241, 445, 605, 688, 919
MalI	GATC	9 cut(s)	50, 108, 191, 351, 455, 461, 641, 840, 893
MauBI	CGCGCGCG	1 cut(s)	483
MboI	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
MboII	GAAGA	3 cut(s)	163, 634, 892
MflI	RGATCY	3 cut(s)	48, 106, 639
MhlI	GDGCHC	3 cut(s)	667, 930, 972
MlsI	TGGCCA	1 cut(s)	845
MluCI	AATT	2 cut(s)	378, 942
MluNI	TGGCCA	1 cut(s)	845
MlyI	GAGTC	2 cut(s)	509, 880
MmeI	TCCRAC	2 cut(s)	370, 919
Mox20I	TGGCCA	1 cut(s)	845
MreI	CGCCGGCG	2 cut(s)	41, 853
MroNI	GCCGGC	3 cut(s)	41, 506, 853
MscI	TGGCCA	1 cut(s)	845
MseI	TTAA	1 cut(s)	1081
MslI	CAYNNNNRTG	1 cut(s)	917
Msp20I	TGGCCA	1 cut(s)	845
MspR9I	CCNGG	8 cut(s)	300, 369, 473, 563, 647, 660, 768, 1006
Mva1269I	GAATGC	1 cut(s)	66
MvaI	CCWGG	2 cut(s)	473, 1006
MvnI	CGCG	9 cut(s)	99, 325, 425, 436, 483, 485, 487, 584, 611
MwoI	GCNNNNNNNGC	9 cut(s)	38, 72, 405, 425, 579, 800, 856, 859, 976
NaeI	GCCGGC	3 cut(s)	43, 508, 855
NciI	CCSGG	6 cut(s)	300, 369, 563, 647, 660, 768
NdeII	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
NgoMIV	GCCGGC	3 cut(s)	41, 506, 853
NlaIII	CATG	2 cut(s)	606, 1045
NlaIV	GGNNCC	4 cut(s)	76, 121, 269, 958
NmeAIII	GCCGAG	4 cut(s)	51, 106, 157, 661
NmuCI	GTSAC	5 cut(s)	241, 445, 605, 688, 919
NspI	RCATGY	1 cut(s)	606
PaeR7I	CTCGAG	1 cut(s)	548
PauI	GCGCGC	2 cut(s)	97, 483
PciI	ACATGT	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	5 cut(s)	78, 354, 396, 678, 723
PctI	GAATGC	1 cut(s)	66
PdiI	GCCGGC	3 cut(s)	43, 508, 855
PfeI	GAWTC	3 cut(s)	744, 1023, 1045
Pfl23II	CGTACG	1 cut(s)	678
PflMI	CCANNNNNTGG	1 cut(s)	916
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	7 cut(s)	179, 398, 427, 852, 861, 934, 1013
PleI	GAGTC	2 cut(s)	509, 879
PmaCI	CACGTG	1 cut(s)	727
PmlI	CACGTG	1 cut(s)	727
PpsI	GAGTC	2 cut(s)	509, 879
Ppu21I	YACGTR	2 cut(s)	673, 727
PscI	ACATGT	1 cut(s)	602
Psp6I	CCWGG	2 cut(s)	471, 1004
PspCI	CACGTG	1 cut(s)	727
PspGI	CCWGG	2 cut(s)	471, 1004
PspLI	CGTACG	1 cut(s)	678
PspN4I	GGNNCC	4 cut(s)	76, 121, 269, 958
PspPI	GGNCC	6 cut(s)	74, 165, 268, 407, 427, 438
PsuI	RGATCY	3 cut(s)	48, 106, 639
PteI	GCGCGC	2 cut(s)	97, 483
RigI	GGCCGGCC	1 cut(s)	510
RsaI	GTAC	7 cut(s)	25, 470, 587, 632, 671, 680, 704
RsaNI	GTAC	7 cut(s)	24, 469, 586, 631, 670, 679, 703
RseI	CAYNNNNRTG	1 cut(s)	917
SaqAI	TTAA	1 cut(s)	1081
SatI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
Sau3AI	GATC	9 cut(s)	48, 106, 189, 349, 453, 459, 639, 838, 891
Sau96I	GGNCC	6 cut(s)	74, 165, 268, 407, 427, 438
SchI	GAGTC	2 cut(s)	509, 880
ScrFI	CCNGG	8 cut(s)	300, 369, 473, 563, 647, 660, 768, 1006
SduI	GDGCHC	3 cut(s)	667, 930, 972
SexAI	ACCWGGT	1 cut(s)	471
SfaNI	GCATC	4 cut(s)	383, 543, 966, 1026
Sfr274I	CTCGAG	1 cut(s)	548
SgrAI	CRCCGGYG	2 cut(s)	41, 853
SinI	GGWCC	1 cut(s)	165
SlaI	CTCGAG	1 cut(s)	548
SmiMI	CAYNNNNRTG	1 cut(s)	917
SmlI	CTYRAG	1 cut(s)	548
SmoI	CTYRAG	1 cut(s)	548
Sse9I	AATT	2 cut(s)	378, 942
SspI	AATATT	1 cut(s)	161
StyD4I	CCNGG	8 cut(s)	298, 367, 471, 561, 645, 658, 766, 1004
StyI	CCWWGG	1 cut(s)	951
TaaI	ACNGT	2 cut(s)	925, 961
TaiI	ACGT	4 cut(s)	390, 447, 675, 729
TaqII	GACCGA	1 cut(s)	390
TasI	AATT	2 cut(s)	378, 942
TatI	WGTACW	1 cut(s)	702
TauI	GCSGC	6 cut(s)	180, 399, 428, 853, 935, 1014
TfiI	GAWTC	3 cut(s)	744, 1023, 1045
Tru1I	TTAA	1 cut(s)	1081
Tru9I	TTAA	1 cut(s)	1081
TseFI	GTSAC	5 cut(s)	241, 445, 605, 688, 919
TseI	GCWGC	1 cut(s)	859
Tsp45I	GTSAC	5 cut(s)	241, 445, 605, 688, 919
TspGWI	ACGGA	1 cut(s)	800
Van91I	CCANNNNNTGG	1 cut(s)	916
VneI	GTGCAC	1 cut(s)	968
VpaK11BI	GGWCC	1 cut(s)	165
XagI	CCTNNNNNAGG	1 cut(s)	332
XapI	RAATTY	1 cut(s)	378
XceI	RCATGY	1 cut(s)	606
XcmI	CCANNNNNNNNNTGG	1 cut(s)	958
XhoI	CTCGAG	1 cut(s)	548
XmiI	GTMKAC	1 cut(s)	222
ZraI	GACGTC	1 cut(s)	388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.