Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004681

Physical Location & Seq

Genomic Coordinates Forward (+)

367822 .. 369818

Gene Structure 1997 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1006600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGACTCTCTCACTCTCTTTTGTACGGGCGCTCTCCTCGCCGGCGGGATCTATTGGTTTGTCTGCATTCTTGGCCCTGCCGAGCGAAAAGGCAAACGTGCCATGGATCTCTCCGGTGGCTCCATCTCCGCCGAGAAAGTTCAAGATAGCTACGACAAATATTGGTCCTTTTTCCGCCGCCCAAAAGAGATCGAAACGGCCGAGAAAGTCCCCGACTTTGTGGACACCTTTTACAACTTAGTCACCGATATTTACGAGTGGGGTTGGGGCCAGTCCTTTCACTTCTCCCCGCACGTCCCCGGCAAGTCCCACAAGGACGCCACGCGCCTCCACGAGGAGATGGCCGTCGATCTCATCGACGTGAAACCCGGAGACCGAATTCTCGACGTCGGATGCGGCGTAGGTGGGCCTATGCGTGCCATCGCGGCCCACTCGCGCGCCAACGTCACCGGGATCACGATCAACGAGTACCAGGTTAAACGGGCGCGTCAGCACAATAAGAAGGCCGGCCTCGAATCGCTCTGCGAGGTCGTCTGCGGGAACTTCCTCGAGATGCCGTTCCCGGAGAACAGCTTTGACGGCGCGTACTCAATCGAGGCGACATGCCACGCGCCGAAGTTGGAGGAGGTGTACGCTGAGATCTTCAGGGTTCTGAAGCCGGGGGCTCTGTACGTGTCGTACGAGTGGGTGACGACGGACAAGTACAAGGGTGACGACGCCGAACACATGGATGTCATTCAGGGGATCGAGAGAGGGGACGCCCTGCCGGGACTCAGGAGCTATACGGATATTGCGGAGACGGCGAGGAAGGTCGGGTTCGAGGTGGTGAAGGAGAAAGATCTGGCCAAGCCGCCGGCGCAGCCGTGGTGGAATCGATTGAAGATGGGGAGGATCGCATACTGGCGCAACCACATTCTGGTGACGGTGCTCGCCGCCGTCGGAATTGCACCGAAAGGAACCGTGGATGTGCACGAGATGCTGTTCAAAACCGCGGACTTTTTGACCAGGGGCGGCGAAACCGGAATCTTCAGTCCGATGCACATGATTCTCTGCCGGAAGCCCGAGACAGCCAAATCATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006275 GO:0006629 GO:0006694 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008156 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009825 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010051 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0012505 GO:0016049 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045786 GO:0045934 GO:0048519 GO:0048523 GO:0048856 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090329 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko00100 ko01110 map00100 map01110

Pfam Domains

Protein Families

Methyltransf_11 Sterol_MT_C

Protein Analysis

361

Amino Acids

40.25

Weight (kDa)

6.28

Isoelectric Point (pI)

34.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	76 - 282	2.2e-16	Mycolic acid cyclopropane synthetase
Ubie_methyltran	PF01209	110 - 228	1.7e-10	ubiE/COQ5 methyltransferase family
Methyltransf_31	PF13847	122 - 229	2.5e-17	Methyltransferase domain
Methyltransf_23	PF13489	122 - 277	1.5e-09	Methyltransferase domain
Methyltransf_25	PF13649	127 - 221	9.4e-21	Methyltransferase domain
Methyltransf_11	PF08241	128 - 224	6.5e-24	Methyltransferase domain
Methyltransf_12	PF08242	128 - 223	3.9e-12	Methyltransferase domain
Sterol_MT_C	PF08498	292 - 355	9.9e-23	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004681)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20330 AT1G76090
fragaria_vesca	FvH4_2g37920
malus_domestica	MD08G1007400.v1.1 MD15G1006600.v1.1
prunus_persica	Prupe.1G359200_v2.0.a1
pyrus_communis	pycom08g00600 pycom15g00520
rosa_chinensis	RchiOBHm_Chr4g0386051 RchiOBHm_Chr6g0303691
rosa_laevigata	RLG00000010176 RLG00000011056
rosa_multiflora	Rmu_co8442993.1_g000001 Rmu_sc0000472.1_g000013 Rmu_sc0002413.1_g000008 Rmu_sc0026342.1_g000001 Rmu_ssc0000114.1_g000012
rosa_roxburghii	Rroxscaffold_5G00333810 Rroxscaffold_7G00164480
rosa_rugosa	Rorug03G0306500 Rorug06G0324000
rosa_samantha	Rh4AG010400 Rh4BG007500 Rh4CG011100 Rh4DG008800 Rh6AG438300 Rh6BG464800 Rh6CG450600 Rh6DG437200
rosa_wichuraiana	Rw4G000100 Rw6G037930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	390
AccB7I	CCANNNNNTGG	1 cut(s)	916
AccII	CGCG	8 cut(s)	325, 425, 436, 438, 487, 584, 611, 992
AclWI	GGATC	5 cut(s)	56, 114, 461, 752, 899
AcoI	YGGCCR	3 cut(s)	198, 342, 843
AcsI	RAATTY	1 cut(s)	378
AcuI	CTGAAG	3 cut(s)	628, 674, 1012
AcyI	GRCGYC	4 cut(s)	318, 387, 717, 759
AfaI	GTAC	7 cut(s)	25, 470, 587, 632, 671, 680, 704
AfiI	CCNNNNNNNGG	2 cut(s)	334, 916
AflIII	ACRYGT	1 cut(s)	672
AgsI	TTSAA	3 cut(s)	143, 880, 985
AjiI	CACGTC	2 cut(s)	295, 361
AjnI	CCWGG	2 cut(s)	471, 1004
AluBI	AGCT	3 cut(s)	150, 573, 780
AluI	AGCT	3 cut(s)	150, 573, 780
Alw21I	GWGCWC	2 cut(s)	930, 972
Alw26I	GTCTC	3 cut(s)	366, 791, 1058
Alw44I	GTGCAC	1 cut(s)	968
AlwI	GGATC	5 cut(s)	56, 114, 461, 752, 899
Ama87I	CYCGRG	2 cut(s)	548, 1061
AoxI	GGCC	9 cut(s)	73, 198, 268, 342, 407, 426, 504, 508, 843
ApaLI	GTGCAC	1 cut(s)	968
ApeKI	GCWGC	1 cut(s)	859
ApoI	RAATTY	1 cut(s)	378
AspLEI	GCGC	9 cut(s)	32, 327, 438, 440, 487, 584, 613, 859, 906
AspS9I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
AsuC2I	CCSGG	6 cut(s)	300, 369, 451, 563, 660, 768
AsuHPI	GGTGA	6 cut(s)	235, 439, 700, 722, 838, 931
AvaI	CYCGRG	2 cut(s)	548, 1061
AvaII	GGWCC	1 cut(s)	165
BaeGI	GKGCMC	1 cut(s)	972
BalI	TGGCCA	1 cut(s)	845
BanII	GRGCYC	1 cut(s)	667
BauI	CACGAG	2 cut(s)	332, 971
Bbv12I	GWGCWC	2 cut(s)	930, 972
BbvI	GCAGC	1 cut(s)	871
BccI	CCATC	4 cut(s)	131, 334, 428, 877
BceAI	ACGGC	7 cut(s)	213, 329, 541, 595, 816, 847, 920
BciT130I	CCWGG	2 cut(s)	473, 1006
BcnI	CCSGG	6 cut(s)	300, 369, 451, 563, 660, 768
BcoDI	GTCTC	3 cut(s)	366, 791, 1058
BfoI	RGCGCY	1 cut(s)	33
BglI	GCCNNNNNGGC	1 cut(s)	425
BglII	AGATCT	2 cut(s)	639, 838
BisI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
BlsI	GCNGC	7 cut(s)	179, 398, 427, 852, 861, 934, 1013
Bme1390I	CCNGG	8 cut(s)	300, 369, 451, 473, 563, 660, 768, 1006
Bme18I	GGWCC	1 cut(s)	165
BmeT110I	CYCGRG	2 cut(s)	548, 1061
BmgBI	CACGTC	2 cut(s)	295, 361
BmgT120I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
BmiI	GGNNCC	3 cut(s)	121, 269, 958
BmrFI	CCNGG	8 cut(s)	300, 369, 451, 473, 563, 660, 768, 1006
BmsI	GCATC	4 cut(s)	383, 543, 966, 1026
BpuMI	CCSGG	6 cut(s)	300, 369, 451, 563, 660, 768
Bsa29I	ATCGAT	1 cut(s)	874
BsaAI	YACGTR	1 cut(s)	673
BsaBI	GATNNNNATC	1 cut(s)	458
BsaHI	GRCGYC	4 cut(s)	318, 387, 717, 759
BsaI	GGTCTC	1 cut(s)	366
BsaJI	CCNNGG	7 cut(s)	102, 298, 659, 863, 960, 990, 1005
BsaWI	WCCGGW	2 cut(s)	113, 1019
BsaXI	ACNNNNNCTCC	2 cut(s)	614, 644
Bsc4I	CCNNNNNNNGG	2 cut(s)	334, 916
Bse118I	RCCGGY	3 cut(s)	41, 506, 853
Bse1I	ACTGG	2 cut(s)	271, 905
Bse8I	GATNNNNATC	1 cut(s)	458
BseBI	CCWGG	2 cut(s)	473, 1006
BseCI	ATCGAT	1 cut(s)	874
BseDI	CCNNGG	7 cut(s)	102, 298, 659, 863, 960, 990, 1005
BseGI	GGATG	3 cut(s)	398, 736, 970
BseJI	GATNNNNATC	1 cut(s)	458
BseLI	CCNNNNNNNGG	2 cut(s)	334, 916
BseMII	CTCAG	2 cut(s)	627, 787
BseNI	ACTGG	2 cut(s)	271, 905
BsePI	GCGCGC	1 cut(s)	436
BseRI	GAGGAG	3 cut(s)	26, 350, 638
BseSI	GKGCMC	1 cut(s)	972
BseX3I	CGGCCG	1 cut(s)	198
BseXI	GCAGC	1 cut(s)	871
Bsh1236I	CGCG	8 cut(s)	325, 425, 436, 438, 487, 584, 611, 992
Bsh1285I	CGRYCG	1 cut(s)	201
BshFI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 845
BshVI	ATCGAT	1 cut(s)	874
BsiEI	CGRYCG	1 cut(s)	201
BsiHKAI	GWGCWC	2 cut(s)	930, 972
BsiHKCI	CYCGRG	2 cut(s)	548, 1061
BsiWI	CGTACG	1 cut(s)	678
BslFI	GGGAC	5 cut(s)	194, 281, 292, 770, 783
BslI	CCNNNNNNNGG	2 cut(s)	334, 916
BsmAI	GTCTC	3 cut(s)	366, 791, 1058
BsmBI	CGTCTC	1 cut(s)	791
BsmFI	GGGAC	5 cut(s)	194, 281, 292, 770, 783
BsmI	GAATGC	1 cut(s)	66
BsnI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 845
Bso31I	GGTCTC	1 cut(s)	366
BsoBI	CYCGRG	2 cut(s)	548, 1061
Bsp1286I	GDGCHC	3 cut(s)	667, 930, 972
Bsp19I	CCATGG	1 cut(s)	102
BspANI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 845
BspCNI	CTCAG	2 cut(s)	628, 786
BspDI	ATCGAT	1 cut(s)	874
BspFNI	CGCG	8 cut(s)	325, 425, 436, 438, 487, 584, 611, 992
BspLI	GGNNCC	3 cut(s)	121, 269, 958
BspPI	GGATC	5 cut(s)	56, 114, 461, 752, 899
BspTNI	GGTCTC	1 cut(s)	366
BsrFI	RCCGGY	3 cut(s)	41, 506, 853
BsrI	ACTGG	2 cut(s)	271, 905
BssAI	RCCGGY	3 cut(s)	41, 506, 853
BssECI	CCNNGG	7 cut(s)	102, 298, 659, 863, 960, 990, 1005
BssHII	GCGCGC	1 cut(s)	436
BssNI	GRCGYC	4 cut(s)	318, 387, 717, 759
BssSI	CACGAG	2 cut(s)	332, 971
BssT1I	CCWWGG	1 cut(s)	102
Bst2BI	CACGAG	2 cut(s)	332, 971
Bst2UI	CCWGG	2 cut(s)	473, 1006
Bst4CI	ACNGT	2 cut(s)	925, 961
BstACI	GRCGYC	4 cut(s)	318, 387, 717, 759
BstAPI	GCANNNNNTGC	1 cut(s)	976
BstBAI	YACGTR	1 cut(s)	673
BstC8I	GCNNGC	6 cut(s)	43, 417, 438, 508, 855, 930
BstDEI	CTNAG	3 cut(s)	238, 636, 773
BstDSI	CCRYGG	4 cut(s)	102, 863, 960, 990
BstENI	CCTNNNNNAGG	1 cut(s)	332
BstF5I	GGATG	3 cut(s)	398, 736, 970
BstFNI	CGCG	8 cut(s)	325, 425, 436, 438, 487, 584, 611, 992
BstH2I	RGCGCY	1 cut(s)	33
BstHHI	GCGC	9 cut(s)	32, 327, 438, 440, 487, 584, 613, 859, 906
BstMAI	GTCTC	3 cut(s)	366, 791, 1058
BstMCI	CGRYCG	1 cut(s)	201
BstMWI	GCNNNNNNNGC	8 cut(s)	38, 72, 425, 579, 800, 856, 859, 976
BstNI	CCWGG	2 cut(s)	473, 1006
BstNSI	RCATGY	1 cut(s)	606
BstSCI	CCNGG	8 cut(s)	298, 367, 449, 471, 561, 658, 766, 1004
BstSLI	GKGCMC	1 cut(s)	972
BstUI	CGCG	8 cut(s)	325, 425, 436, 438, 487, 584, 611, 992
BstV1I	GCAGC	1 cut(s)	871
BstX2I	RGATCY	4 cut(s)	48, 106, 639, 838
BstYI	RGATCY	4 cut(s)	48, 106, 639, 838
BstZI	CGGCCG	1 cut(s)	198
Bsu15I	ATCGAT	1 cut(s)	874
BsuRI	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 845
BsuTUI	ATCGAT	1 cut(s)	874
BtgI	CCRYGG	4 cut(s)	102, 863, 960, 990
BtgZI	GCGATG	1 cut(s)	406
BtrI	CACGTC	2 cut(s)	295, 361
BtsCI	GGATG	3 cut(s)	398, 736, 970
Cac8I	GCNNGC	6 cut(s)	43, 417, 438, 508, 855, 930
CfoI	GCGC	9 cut(s)	32, 327, 438, 440, 487, 584, 613, 859, 906
Cfr10I	RCCGGY	3 cut(s)	41, 506, 853
Cfr13I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
Cfr42I	CCGCGG	1 cut(s)	993
ClaI	ATCGAT	1 cut(s)	874
CseI	GACGC	4 cut(s)	326, 476, 725, 767
CsiI	ACCWGGT	1 cut(s)	471
Csp6I	GTAC	7 cut(s)	24, 469, 586, 631, 670, 679, 703
CviAII	CATG	4 cut(s)	103, 603, 727, 1042
CviQI	GTAC	7 cut(s)	24, 469, 586, 631, 670, 679, 703
DdeI	CTNAG	3 cut(s)	238, 636, 773
EaeI	YGGCCR	3 cut(s)	198, 342, 843
EagI	CGGCCG	1 cut(s)	198
EciI	GGCGGA	2 cut(s)	118, 164
EclXI	CGGCCG	1 cut(s)	198
Eco130I	CCWWGG	1 cut(s)	102
Eco24I	GRGCYC	1 cut(s)	667
Eco31I	GGTCTC	1 cut(s)	366
Eco47I	GGWCC	1 cut(s)	165
Eco52I	CGGCCG	1 cut(s)	198
Eco57I	CTGAAG	3 cut(s)	628, 674, 1012
Eco88I	CYCGRG	2 cut(s)	548, 1061
EcoNI	CCTNNNNNAGG	1 cut(s)	332
EcoRI	GAATTC	1 cut(s)	378
EcoRII	CCWGG	2 cut(s)	471, 1004
EcoT14I	CCWWGG	1 cut(s)	102
EcoT38I	GRGCYC	1 cut(s)	667
ErhI	CCWWGG	1 cut(s)	102
Esp3I	CGTCTC	1 cut(s)	791
FaeI	CATG	4 cut(s)	106, 606, 730, 1045
FaiI	YATR	7 cut(s)	104, 413, 604, 728, 783, 898, 1043
FaqI	GGGAC	5 cut(s)	194, 281, 292, 770, 783
FatI	CATG	4 cut(s)	102, 602, 726, 1041
FauI	CCCGC	3 cut(s)	38, 297, 530
Fnu4HI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
FokI	GGATG	3 cut(s)	405, 743, 977
FriOI	GRGCYC	1 cut(s)	667
FseI	GGCCGGCC	1 cut(s)	510
Fsp4HI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
GlaI	GCGC	9 cut(s)	31, 326, 437, 439, 486, 583, 612, 858, 905
GluI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
HaeII	RGCGCY	1 cut(s)	33
HaeIII	GGCC	9 cut(s)	75, 200, 270, 344, 409, 428, 506, 510, 845
HgaI	GACGC	4 cut(s)	326, 476, 725, 767
HhaI	GCGC	9 cut(s)	32, 327, 438, 440, 487, 584, 613, 859, 906
Hin1I	GRCGYC	4 cut(s)	318, 387, 717, 759
Hin1II	CATG	4 cut(s)	106, 606, 730, 1045
Hin6I	GCGC	9 cut(s)	30, 325, 436, 438, 485, 582, 611, 857, 904
HinP1I	GCGC	9 cut(s)	30, 325, 436, 438, 485, 582, 611, 857, 904
HinfI	GANTC	6 cut(s)	5, 515, 771, 871, 1023, 1045
HphI	GGTGA	6 cut(s)	235, 439, 700, 722, 838, 931
Hpy166II	GTNNAC	3 cut(s)	223, 631, 970
Hpy188I	TCNGA	4 cut(s)	392, 654, 941, 1035
Hpy188III	TCNNGA	6 cut(s)	143, 383, 457, 550, 748, 775
Hpy8I	GTNNAC	3 cut(s)	223, 631, 970
Hpy99I	CGWCG	7 cut(s)	350, 362, 389, 392, 697, 719, 941
HpyAV	CCTTC	3 cut(s)	496, 802, 823
HpyCH4III	ACNGT	2 cut(s)	925, 961
HpyCH4IV	ACGT	6 cut(s)	97, 294, 360, 387, 444, 672
HpyCH4V	TGCA	4 cut(s)	66, 947, 970, 1039
HpyF10VI	GCNNNNNNNGC	8 cut(s)	38, 72, 425, 579, 800, 856, 859, 976
HpyF3I	CTNAG	3 cut(s)	238, 636, 773
HpySE526I	ACGT	6 cut(s)	97, 294, 360, 387, 444, 672
Hsp92I	GRCGYC	4 cut(s)	318, 387, 717, 759
Hsp92II	CATG	4 cut(s)	106, 606, 730, 1045
HspAI	GCGC	9 cut(s)	30, 325, 436, 438, 485, 582, 611, 857, 904
KroI	GCCGGC	3 cut(s)	41, 506, 853
KroNI	GCCGGC	3 cut(s)	43, 508, 855
KspI	CCGCGG	1 cut(s)	993
LmnI	GCTCC	2 cut(s)	125, 777
Lsp1109I	GCAGC	1 cut(s)	871
LweI	GCATC	4 cut(s)	383, 543, 966, 1026
MabI	ACCWGGT	1 cut(s)	471
MaeII	ACGT	6 cut(s)	97, 294, 360, 387, 444, 672
MaeIII	GTNAC	5 cut(s)	241, 445, 688, 710, 919
MboII	GAAGA	3 cut(s)	634, 892, 1018
MflI	RGATCY	4 cut(s)	48, 106, 639, 838
MhlI	GDGCHC	3 cut(s)	667, 930, 972
MlsI	TGGCCA	1 cut(s)	845
MluCI	AATT	2 cut(s)	378, 942
MluNI	TGGCCA	1 cut(s)	845
MlyI	GAGTC	1 cut(s)	765
MmeI	TCCRAC	3 cut(s)	370, 600, 919
Mox20I	TGGCCA	1 cut(s)	845
MreI	CGCCGGCG	2 cut(s)	41, 853
MroNI	GCCGGC	3 cut(s)	41, 506, 853
MscI	TGGCCA	1 cut(s)	845
MseI	TTAA	2 cut(s)	477, 1081
MslI	CAYNNNNRTG	3 cut(s)	359, 729, 917
Msp20I	TGGCCA	1 cut(s)	845
MspA1I	CMGCKG	1 cut(s)	992
MspR9I	CCNGG	8 cut(s)	300, 369, 451, 473, 563, 660, 768, 1006
Mva1269I	GAATGC	1 cut(s)	66
MvaI	CCWGG	2 cut(s)	473, 1006
MvnI	CGCG	8 cut(s)	325, 425, 436, 438, 487, 584, 611, 992
MwoI	GCNNNNNNNGC	8 cut(s)	38, 72, 425, 579, 800, 856, 859, 976
NaeI	GCCGGC	3 cut(s)	43, 508, 855
NciI	CCSGG	6 cut(s)	300, 369, 451, 563, 660, 768
NcoI	CCATGG	1 cut(s)	102
NgoMIV	GCCGGC	3 cut(s)	41, 506, 853
NlaIII	CATG	4 cut(s)	106, 606, 730, 1045
NlaIV	GGNNCC	3 cut(s)	121, 269, 958
NmeAIII	GCCGAG	3 cut(s)	106, 157, 226
NmuCI	GTSAC	5 cut(s)	241, 445, 688, 710, 919
NspI	RCATGY	1 cut(s)	606
PaeR7I	CTCGAG	1 cut(s)	548
PauI	GCGCGC	1 cut(s)	436
PcsI	WCGNNNNNNNCGW	4 cut(s)	198, 354, 396, 678
PctI	GAATGC	1 cut(s)	66
PdiI	GCCGGC	3 cut(s)	43, 508, 855
PfeI	GAWTC	4 cut(s)	515, 871, 1023, 1045
Pfl23II	CGTACG	1 cut(s)	678
PflMI	CCANNNNNTGG	1 cut(s)	916
PfoI	TCCNGGA	1 cut(s)	561
PkrI	GCNGC	7 cut(s)	179, 398, 427, 852, 861, 934, 1013
PleI	GAGTC	1 cut(s)	765
PpsI	GAGTC	1 cut(s)	765
Ppu21I	YACGTR	1 cut(s)	673
Psp6I	CCWGG	2 cut(s)	471, 1004
PspGI	CCWGG	2 cut(s)	471, 1004
PspLI	CGTACG	1 cut(s)	678
PspN4I	GGNNCC	3 cut(s)	121, 269, 958
PspPI	GGNCC	5 cut(s)	74, 165, 268, 407, 427
PsuI	RGATCY	4 cut(s)	48, 106, 639, 838
PteI	GCGCGC	1 cut(s)	436
RigI	GGCCGGCC	1 cut(s)	510
RsaI	GTAC	7 cut(s)	25, 470, 587, 632, 671, 680, 704
RsaNI	GTAC	7 cut(s)	24, 469, 586, 631, 670, 679, 703
RseI	CAYNNNNRTG	3 cut(s)	359, 729, 917
SacII	CCGCGG	1 cut(s)	993
SaqAI	TTAA	2 cut(s)	477, 1081
SatI	GCNGC	7 cut(s)	178, 397, 426, 851, 860, 933, 1012
Sau96I	GGNCC	5 cut(s)	74, 165, 268, 407, 427
SchI	GAGTC	1 cut(s)	765
ScrFI	CCNGG	8 cut(s)	300, 369, 451, 473, 563, 660, 768, 1006
SduI	GDGCHC	3 cut(s)	667, 930, 972
SexAI	ACCWGGT	1 cut(s)	471
SfaNI	GCATC	4 cut(s)	383, 543, 966, 1026
Sfr274I	CTCGAG	1 cut(s)	548
Sfr303I	CCGCGG	1 cut(s)	993
SgrAI	CRCCGGYG	2 cut(s)	41, 853
SgrBI	CCGCGG	1 cut(s)	993
SinI	GGWCC	1 cut(s)	165
SlaI	CTCGAG	1 cut(s)	548
SmiMI	CAYNNNNRTG	3 cut(s)	359, 729, 917
SmlI	CTYRAG	1 cut(s)	548
SmoI	CTYRAG	1 cut(s)	548
Sse9I	AATT	2 cut(s)	378, 942
SspI	AATATT	1 cut(s)	161
StyD4I	CCNGG	8 cut(s)	298, 367, 449, 471, 561, 658, 766, 1004
StyI	CCWWGG	1 cut(s)	102
TaaI	ACNGT	2 cut(s)	925, 961
TaiI	ACGT	6 cut(s)	100, 297, 363, 390, 447, 675
TaqII	GACCGA	1 cut(s)	390
TasI	AATT	2 cut(s)	378, 942
TatI	WGTACW	1 cut(s)	702
TauI	GCSGC	6 cut(s)	180, 399, 428, 853, 935, 1014
TfiI	GAWTC	4 cut(s)	515, 871, 1023, 1045
Tru1I	TTAA	2 cut(s)	477, 1081
Tru9I	TTAA	2 cut(s)	477, 1081
TseFI	GTSAC	5 cut(s)	241, 445, 688, 710, 919
TseI	GCWGC	1 cut(s)	859
Tsp45I	GTSAC	5 cut(s)	241, 445, 688, 710, 919
TspGWI	ACGGA	2 cut(s)	710, 800
Van91I	CCANNNNNTGG	1 cut(s)	916
VneI	GTGCAC	1 cut(s)	968
VpaK11BI	GGWCC	1 cut(s)	165
XagI	CCTNNNNNAGG	1 cut(s)	332
XapI	RAATTY	1 cut(s)	378
XceI	RCATGY	1 cut(s)	606
XhoI	CTCGAG	1 cut(s)	548
ZraI	GACGTC	1 cut(s)	388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.