AT1G21010

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012234
Physical Location & Seq
Forward (+)
7346156 .. 7347283
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21010.1

Sequence Viewer

Length: 633 bp
ATGGGAATCTGTGTGTCGTTTCGTCGGGAAGATTCAAATTCTTCACCGACGGTGAAGATCGTGACGGTTAACGGCGATCTCCGTGAATACAATGTTCCGGTGATAGCATCTCAAGTTTTGGAAGCTGAATCAGCGGCAGCTTATTCATCTTCGTCTTCCTCTAGACCTTCTTCATATTTCATCTGCGATTCAGATTCTCTCTACTACGACGATTTCATACCGGCGATCAAATCGGAAGAGCCGCTTCAGGCGGACCAGATCTACTTCGTTCTCCCGATTTCTAAACGTCAGAGTCGTTTAACGGCGTCGGATATGGCGGCTCTCGCCGTTAAAGCAAGCGTCGCGATACAAAACTCTGTTAAGAAAGAATCTCGTCGGCGTAAGAAGGTGAGAATCTCTCCGGTTATGATGTTGACGGGGTCTAACGATTCCGTTAACGGAAATGGAAGTGAAACGACAGTTAAAAAGGGACGGCCGTTTGTGAGTAAAACTGCGCCGGTTAAAGCTTCGAGTGGGATAAACCGGTCCGGTTCGGTTCGTAATTTGAGAAGATACACTTCTAAACGAGCTAAGCTGGCGGTTCGATCTTTCAGGCTAAAACTTTCAACCATCTACGAAGGCTCCGTCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

210

Amino Acids

22.97

Weight (kDa)

10.04

Isoelectric Point (pI)

63.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 206 3.8e-34 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 344
AciI CCGC 5 cut(s) 134, 242, 251, 317, 578
AcoI YGGCCR 1 cut(s) 473
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 305
AgeI ACCGGT 1 cut(s) 522
AgsI TTSAA 2 cut(s) 36, 606
AluBI AGCT 5 cut(s) 125, 140, 506, 569, 574
AluI AGCT 5 cut(s) 125, 140, 506, 569, 574
AoxI GGCC 1 cut(s) 473
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 37
ArsI GACNNNNNNTTYG 1 cut(s) 609
AsiGI ACCGGT 1 cut(s) 522
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 2 cut(s) 253, 525
AsuHPI GGTGA 4 cut(s) 36, 64, 112, 400
AvaII GGWCC 2 cut(s) 253, 525
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 149
BccI CCATC 1 cut(s) 617
BceAI ACGGC 5 cut(s) 88, 311, 318, 460, 488
BfaI CTAG 1 cut(s) 162
BglII AGATCT 1 cut(s) 258
BisI GCNGC 4 cut(s) 135, 138, 242, 318
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 4 cut(s) 136, 139, 243, 319
Bme18I GGWCC 2 cut(s) 253, 525
BmgT120I GGNCC 2 cut(s) 253, 525
BmiI GGNNCC 1 cut(s) 622
BmsI GCATC 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 147
BplI GAGNNNNNCTC 2 cut(s) 382, 414
Bpu1102I GCTNAGC 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 305
BsaWI WCCGGW 4 cut(s) 97, 400, 522, 527
BsaXI ACNNNNNCTCC 1 cut(s) 605
Bse118I RCCGGY 3 cut(s) 220, 496, 522
BseX3I CGGCCG 1 cut(s) 473
BseXI GCAGC 1 cut(s) 149
Bsh1236I CGCG 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 1 cut(s) 475
BshTI ACCGGT 1 cut(s) 522
BsiEI CGRYCG 1 cut(s) 476
BsiSI CCGG 6 cut(s) 98, 221, 401, 497, 523, 528
BslFI GGGAC 1 cut(s) 483
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 1 cut(s) 475
Bsp143I GATC 5 cut(s) 57, 76, 225, 258, 584
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp68I TCGCGA 1 cut(s) 344
BspACI CCGC 5 cut(s) 134, 242, 251, 317, 578
BspANI GGCC 1 cut(s) 475
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 1 cut(s) 622
BspQI GCTCTTC 1 cut(s) 231
BsrFI RCCGGY 3 cut(s) 220, 496, 522
BssAI RCCGGY 3 cut(s) 220, 496, 522
BssMI GATC 5 cut(s) 57, 76, 225, 258, 584
BssNI GRCGYC 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 52, 67, 460
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 337, 576
BstDEI CTNAG 1 cut(s) 570
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 5 cut(s) 60, 79, 228, 261, 587
BstMBI GATC 5 cut(s) 57, 76, 225, 258, 584
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 5 cut(s) 131, 323, 332, 341, 575
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 149
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BstZI CGGCCG 1 cut(s) 473
BsuRI GGCC 1 cut(s) 475
BtuMI TCGCGA 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 337, 576
CfoI GCGC 1 cut(s) 496
Cfr10I RCCGGY 3 cut(s) 220, 496, 522
Cfr13I GGNCC 2 cut(s) 253, 525
CpoI CGGWCCG 1 cut(s) 525
CseI GACGC 2 cut(s) 294, 328
CspAI ACCGGT 1 cut(s) 522
CspI CGGWCCG 1 cut(s) 525
DdeI CTNAG 1 cut(s) 570
DpnI GATC 5 cut(s) 59, 78, 227, 260, 586
DpnII GATC 5 cut(s) 57, 76, 225, 258, 584
EaeI YGGCCR 1 cut(s) 473
EagI CGGCCG 1 cut(s) 473
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 1 cut(s) 266
EclXI CGGCCG 1 cut(s) 473
Eco47I GGWCC 2 cut(s) 253, 525
Eco52I CGGCCG 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 230
FaiI YATR 4 cut(s) 175, 218, 314, 407
FalI AAGNNNNNCTT 4 cut(s) 228, 260, 541, 573
FaqI GGGAC 1 cut(s) 483
Fnu4HI GCNGC 4 cut(s) 135, 138, 242, 318
Fsp4HI GCNGC 4 cut(s) 135, 138, 242, 318
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 495
GluI GCNGC 4 cut(s) 135, 138, 242, 318
HaeIII GGCC 1 cut(s) 475
HapII CCGG 6 cut(s) 98, 221, 401, 497, 523, 528
HgaI GACGC 2 cut(s) 294, 328
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 3 cut(s) 70, 414, 436
HindII GTYRAC 3 cut(s) 70, 414, 436
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 9 cut(s) 6, 32, 128, 188, 194, 292, 368, 393, 428
HpaI GTTAAC 2 cut(s) 70, 436
HpaII CCGG 6 cut(s) 98, 221, 401, 497, 523, 528
HphI GGTGA 4 cut(s) 36, 64, 112, 400
Hpy166II GTNNAC 3 cut(s) 70, 414, 436
Hpy188I TCNGA 4 cut(s) 193, 235, 291, 310
Hpy188III TCNNGA 5 cut(s) 26, 61, 162, 274, 343
Hpy8I GTNNAC 3 cut(s) 70, 414, 436
Hpy99I CGWCG 7 cut(s) 27, 52, 212, 310, 344, 378, 629
HpyAV CCTTC 3 cut(s) 177, 379, 611
HpyCH4III ACNGT 3 cut(s) 52, 67, 460
HpyCH4IV ACGT 1 cut(s) 286
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 323, 332, 341, 575
HpyF3I CTNAG 1 cut(s) 570
HpySE526I ACGT 1 cut(s) 286
Hsp92I GRCGYC 1 cut(s) 305
HspAI GCGC 1 cut(s) 494
KspAI GTTAAC 2 cut(s) 70, 436
Kzo9I GATC 5 cut(s) 57, 76, 225, 258, 584
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 1 cut(s) 626
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 1 cut(s) 116
MaeI CTAG 1 cut(s) 162
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 1 cut(s) 61
MalI GATC 5 cut(s) 59, 78, 227, 260, 586
MboI GATC 5 cut(s) 57, 76, 225, 258, 584
MboII GAAGA 8 cut(s) 33, 41, 67, 141, 147, 162, 248, 561
MflI RGATCY 1 cut(s) 258
MluCI AATT 2 cut(s) 37, 541
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 1 cut(s) 169
MseI TTAA 7 cut(s) 69, 299, 330, 360, 435, 462, 501
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 6 cut(s) 98, 221, 401, 497, 523, 528
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 5 cut(s) 131, 323, 332, 341, 575
NdeII GATC 5 cut(s) 57, 76, 225, 258, 584
NlaIV GGNNCC 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 61
NruI TCGCGA 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 231
PcsI WCGNNNNNNNCGW 2 cut(s) 292, 621
PfeI GAWTC 8 cut(s) 6, 32, 128, 188, 194, 368, 393, 428
PflFI GACNNNGTC 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 522
PkrI GCNGC 4 cut(s) 136, 139, 243, 319
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 622
PspPI GGNCC 2 cut(s) 253, 525
PsuI RGATCY 1 cut(s) 258
PsyI GACNNNGTC 1 cut(s) 418
RruI TCGCGA 1 cut(s) 344
Rsr2I CGGWCCG 1 cut(s) 525
RsrII CGGWCCG 1 cut(s) 525
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 7 cut(s) 69, 299, 330, 360, 435, 462, 501
SatI GCNGC 4 cut(s) 135, 138, 242, 318
Sau3AI GATC 5 cut(s) 57, 76, 225, 258, 584
Sau96I GGNCC 2 cut(s) 253, 525
SchI GAGTC 1 cut(s) 301
SetI ASST 8 cut(s) 127, 142, 169, 289, 390, 508, 571, 576
SfaNI GCATC 1 cut(s) 116
SinI GGWCC 2 cut(s) 253, 525
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
Sse9I AATT 2 cut(s) 37, 541
SsiI CCGC 5 cut(s) 134, 242, 251, 317, 578
SspMI CTAG 1 cut(s) 162
TaaI ACNGT 3 cut(s) 52, 67, 460
TaiI ACGT 1 cut(s) 289
TaqI TCGA 2 cut(s) 509, 583
TasI AATT 2 cut(s) 37, 541
TauI GCSGC 3 cut(s) 137, 244, 320
TfiI GAWTC 8 cut(s) 6, 32, 128, 188, 194, 368, 393, 428
Tru1I TTAA 7 cut(s) 69, 299, 330, 360, 435, 462, 501
Tru9I TTAA 7 cut(s) 69, 299, 330, 360, 435, 462, 501
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 135, 162, 169, 205
TspGWI ACGGA 4 cut(s) 71, 421, 453, 613
Tth111I GACNNNGTC 1 cut(s) 418
VpaK11BI GGWCC 2 cut(s) 253, 525
XapI RAATTY 1 cut(s) 37
XbaI TCTAGA 1 cut(s) 161
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.