AT1G76600

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012234
Physical Location & Seq
Forward (+)
28746833 .. 28747848
1016 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76600.1

Sequence Viewer

Length: 651 bp
ATGGGTCTTTGTGTATCTGTTAATCGAAACGAGTACGTTTCTTCGTCGACGACGGCTAAAATCGTAACAATTAACGGCGATCTCCGTGAATACGATGTACCGGTGTTAGCATCTCAGGTTCTGGAATCTGAATCGACGTCTTCTTCTTCATCATCATCATCTTCTTCTTATTTTCTATGTAATTCCGATTCTCTCTACTACGACGATTTTATCCCAGCGATTGAATCTGATGAGATTCTTCAAGCGAATCAGATCTATTTCGTCCTTCCCATCTCTAAACGACAATACCGTCTCTCTGCCTCGGATATGGCGGCTCTCGCTGTAAAAGCCAGCGTCGCGATTGAGAAAGCAGCGGGGAAGAAAAATCGCCGTCGTAGAAGCGGAAGAATCTCTCCGGTTGTGACGTTGAATCAGGCTAACGATAATCGGATCGCCGCCGTGAACAACAGAATTGGAGGCGAAGCAACGAATATGATGATGCAAAAAGGAAAGTTACCGAATAGAACGACGCCGTTTAAAGATACAAACGGTTATTCTCGGTCTGGTTCGGTTAGGAAATTGAAAAGATATACGTCTGGTCGAGCTAAATTGGCGGTTCGATCGTTTAGACTGAGACTCTCTACTATATACGAAGGCTCTTCGTTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

216

Amino Acids

23.8

Weight (kDa)

9.93

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 211 2.7e-33 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 288
AatII GACGTC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 47
AccII CGCG 1 cut(s) 338
AciI CCGC 5 cut(s) 311, 353, 381, 435, 593
AclWI GGATC 1 cut(s) 437
AcyI GRCGYC 2 cut(s) 137, 509
AfaI GTAC 2 cut(s) 35, 99
AgeI ACCGGT 1 cut(s) 100
AgsI TTSAA 4 cut(s) 224, 242, 409, 562
AluBI AGCT 1 cut(s) 584
AluI AGCT 1 cut(s) 584
Alw26I GTCTC 2 cut(s) 296, 607
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 121
ApeKI GCWGC 1 cut(s) 350
AsiGI ACCGGT 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 278
BceAI ACGGC 5 cut(s) 69, 91, 354, 422, 496
BcoDI GTCTC 2 cut(s) 296, 607
BglII AGATCT 1 cut(s) 252
BisI GCNGC 3 cut(s) 312, 351, 435
BlsI GCNGC 3 cut(s) 313, 352, 436
BmsI GCATC 2 cut(s) 119, 468
BpiI GAAGAC 1 cut(s) 132
BsaHI GRCGYC 2 cut(s) 137, 509
BsaJI CCNNGG 1 cut(s) 300
BsaWI WCCGGW 2 cut(s) 100, 394
Bse118I RCCGGY 1 cut(s) 100
BseDI CCNNGG 1 cut(s) 300
BseMII CTCAG 2 cut(s) 128, 602
BseXI GCAGC 1 cut(s) 362
BseYI CCCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 602
BshTI ACCGGT 1 cut(s) 100
BsiEI CGRYCG 1 cut(s) 602
BsiSI CCGG 2 cut(s) 101, 395
BsmAI GTCTC 2 cut(s) 296, 607
BsmBI CGTCTC 1 cut(s) 296
Bsp143I GATC 4 cut(s) 79, 252, 429, 599
Bsp68I TCGCGA 1 cut(s) 338
BspACI CCGC 5 cut(s) 311, 353, 381, 435, 593
BspCNI CTCAG 2 cut(s) 127, 603
BspFNI CGCG 1 cut(s) 338
BspPI GGATC 1 cut(s) 437
BspQI GCTCTTC 1 cut(s) 643
BsrFI RCCGGY 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 4 cut(s) 79, 252, 429, 599
BssNI GRCGYC 2 cut(s) 137, 509
Bst4CI ACNGT 2 cut(s) 290, 530
Bst6I CTCTTC 1 cut(s) 643
BstACI GRCGYC 2 cut(s) 137, 509
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 2 cut(s) 114, 611
BstFNI CGCG 1 cut(s) 338
BstKTI GATC 4 cut(s) 82, 255, 432, 602
BstMAI GTCTC 2 cut(s) 296, 607
BstMBI GATC 4 cut(s) 79, 252, 429, 599
BstMCI CGRYCG 1 cut(s) 602
BstMWI GCNNNNNNNGC 4 cut(s) 317, 326, 335, 590
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 362
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BtuMI TCGCGA 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 331
CaiI CAGNNNCTG 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 100
CseI GACGC 2 cut(s) 322, 517
Csp6I GTAC 2 cut(s) 34, 98
CspAI ACCGGT 1 cut(s) 100
CviJI RGCY 6 cut(s) 56, 314, 329, 416, 584, 636
CviKI_1 RGCY 6 cut(s) 56, 314, 329, 416, 584, 636
CviQI GTAC 2 cut(s) 34, 98
DdeI CTNAG 2 cut(s) 114, 611
DpnI GATC 4 cut(s) 81, 254, 431, 601
DpnII GATC 4 cut(s) 79, 252, 429, 599
DraI TTTAAA 1 cut(s) 517
DrdI GACNNNNNNGTC 1 cut(s) 288
DseDI GACNNNNNNGTC 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 643
EarI CTCTTC 1 cut(s) 643
Esp3I CGTCTC 1 cut(s) 296
FaiI YATR 6 cut(s) 178, 308, 473, 570, 626, 628
FauI CCCGC 1 cut(s) 346
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 3 cut(s) 312, 351, 435
Fsp4HI GCNGC 3 cut(s) 312, 351, 435
GluI GCNGC 3 cut(s) 312, 351, 435
GsaI CCCAGC 1 cut(s) 218
HapII CCGG 2 cut(s) 101, 395
HgaI GACGC 2 cut(s) 322, 517
Hin1I GRCGYC 2 cut(s) 137, 509
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 9 cut(s) 125, 131, 188, 224, 235, 247, 387, 409, 615
HpaII CCGG 2 cut(s) 101, 395
Hpy166II GTNNAC 2 cut(s) 48, 442
Hpy188I TCNGA 6 cut(s) 130, 187, 229, 252, 304, 429
Hpy188III TCNNGA 2 cut(s) 122, 337
Hpy8I GTNNAC 2 cut(s) 48, 442
Hpy99I CGWCG 8 cut(s) 49, 52, 55, 139, 206, 338, 375, 511
HpyAV CCTTC 2 cut(s) 275, 626
HpyCH4III ACNGT 2 cut(s) 290, 530
HpyCH4IV ACGT 4 cut(s) 36, 137, 404, 572
HpyCH4V TGCA 1 cut(s) 481
HpyF10VI GCNNNNNNNGC 4 cut(s) 317, 326, 335, 590
HpyF3I CTNAG 2 cut(s) 114, 611
HpySE526I ACGT 4 cut(s) 36, 137, 404, 572
Hsp92I GRCGYC 2 cut(s) 137, 509
Kzo9I GATC 4 cut(s) 79, 252, 429, 599
LguI GCTCTTC 1 cut(s) 643
LpnPI CCDG 9 cut(s) 101, 107, 114, 228, 343, 398, 408, 528, 561
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 2 cut(s) 119, 468
MaeII ACGT 4 cut(s) 36, 137, 404, 572
MaeIII GTNAC 3 cut(s) 64, 400, 492
MalI GATC 4 cut(s) 81, 254, 431, 601
MboI GATC 4 cut(s) 79, 252, 429, 599
MflI RGATCY 1 cut(s) 252
MluCI AATT 6 cut(s) 69, 181, 450, 557, 587, 646
MlyI GAGTC 1 cut(s) 609
MnlI CCTC 2 cut(s) 310, 449
MseI TTAA 4 cut(s) 21, 72, 516, 645
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 2 cut(s) 101, 395
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 317, 326, 335, 590
NdeII GATC 4 cut(s) 79, 252, 429, 599
NmuCI GTSAC 1 cut(s) 400
NruI TCGCGA 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 643
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PfeI GAWTC 8 cut(s) 125, 131, 188, 224, 235, 247, 387, 409
PinAI ACCGGT 1 cut(s) 100
PkrI GCNGC 3 cut(s) 313, 352, 436
Ple19I CGATCG 1 cut(s) 602
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 252
PvuI CGATCG 1 cut(s) 602
RruI TCGCGA 1 cut(s) 338
RsaI GTAC 2 cut(s) 35, 99
RsaNI GTAC 2 cut(s) 34, 98
SalI GTCGAC 1 cut(s) 46
SapI GCTCTTC 1 cut(s) 643
SaqAI TTAA 4 cut(s) 21, 72, 516, 645
SatI GCNGC 3 cut(s) 312, 351, 435
Sau3AI GATC 4 cut(s) 79, 252, 429, 599
SchI GAGTC 1 cut(s) 609
SetI ASST 6 cut(s) 39, 120, 140, 407, 575, 586
SfaNI GCATC 2 cut(s) 119, 468
SgrDI CGTCGACG 1 cut(s) 46
Sse9I AATT 6 cut(s) 69, 181, 450, 557, 587, 646
SsiI CCGC 5 cut(s) 311, 353, 381, 435, 593
TaaI ACNGT 2 cut(s) 290, 530
TaiI ACGT 4 cut(s) 39, 140, 407, 575
TaqI TCGA 5 cut(s) 25, 47, 134, 580, 598
TaqII GACCGA 1 cut(s) 528
TasI AATT 6 cut(s) 69, 181, 450, 557, 587, 646
TauI GCSGC 2 cut(s) 314, 437
TfiI GAWTC 8 cut(s) 125, 131, 188, 224, 235, 247, 387, 409
Tru1I TTAA 4 cut(s) 21, 72, 516, 645
Tru9I TTAA 4 cut(s) 21, 72, 516, 645
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 1 cut(s) 138
TspGWI ACGGA 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 47
ZraI GACGTC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.