Rroxscaffold_3G00241920

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012234
Physical Location & Seq
Reverse (-)
34234673 .. 34235617
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00241920.1

Sequence Viewer

Length: 363 bp
ATGGCCGCTTTGGCTGTCAGGGCCAGCCTCGCCCTCCAAAATGCGTCCAAGGCTTCTTCCTCGGGAAAAAAGGATCGTACTCGTCGCCGGAACAAATACCGGGTTTCTCCCATTCTCGTTGTCAAGTCGGAGCCCAGATCGGAGTACGATTTTGTGGATTACGAGGCTGATTTTACGATTGGGCCCTCCAATTCTCTTATGAAGAAGAAGGTGATGGCGCCGGAGAAGGCTGCTTTGGGGGTTTCGAGATCTGGGTCCGTCCGGAAGTTGCAGAGATACTCATCTAAAAGAGTATTGAAGAGGGCCGTTCGGTCGTTTAAACTCAGATTGACCCCAATACAAGAAGGTACCGAGGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

120

Amino Acids

13.45

Weight (kDa)

10.88

Isoelectric Point (pI)

61.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 2 cut(s) 217, 347
AccIII TCCGGA 1 cut(s) 261
AciI CCGC 1 cut(s) 6
AclWI GGATC 1 cut(s) 81
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 3 cut(s) 79, 146, 349
AgsI TTSAA 1 cut(s) 298
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AlwI GGATC 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 61
Aor13HI TCCGGA 1 cut(s) 261
AoxI GGCC 4 cut(s) 3, 21, 182, 303
ApaI GGGCCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 230
Asp718I GGTACC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 5 cut(s) 21, 182, 183, 255, 303
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 186
BaeI ACNNNNGTAYC 1 cut(s) 339
BanI GGYRCC 2 cut(s) 217, 347
BanII GRGCYC 2 cut(s) 135, 186
BbvI GCAGC 1 cut(s) 217
BccI CCATC 1 cut(s) 208
BceAI ACGGC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 101
BfoI RGCGCY 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 11
BglII AGATCT 1 cut(s) 248
BisI GCNGC 2 cut(s) 6, 231
BlsI GCNGC 2 cut(s) 7, 232
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 61
BmgT120I GGNCC 5 cut(s) 21, 182, 183, 255, 303
BmiI GGNNCC 5 cut(s) 132, 184, 219, 256, 349
BmrFI CCNGG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 280
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 3 cut(s) 48, 60, 351
BsaWI WCCGGW 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 280
BseAI TCCGGA 1 cut(s) 261
BseDI CCNNGG 3 cut(s) 48, 60, 351
BseJI GATNNNNATC 1 cut(s) 280
BseMII CTCAG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 314
BshFI GGCC 4 cut(s) 5, 23, 184, 305
BshNI GGYRCC 2 cut(s) 217, 347
BsiEI CGRYCG 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 4 cut(s) 88, 100, 221, 262
BsnI GGCC 4 cut(s) 5, 23, 184, 305
BsoBI CYCGRG 1 cut(s) 61
Bsp120I GGGCCC 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 135, 186
Bsp13I TCCGGA 1 cut(s) 261
Bsp143I GATC 3 cut(s) 73, 137, 248
BspACI CCGC 1 cut(s) 6
BspANI GGCC 4 cut(s) 5, 23, 184, 305
BspCNI CTCAG 1 cut(s) 336
BspEI TCCGGA 1 cut(s) 261
BspLI GGNNCC 5 cut(s) 132, 184, 219, 256, 349
BspPI GGATC 1 cut(s) 81
BspT107I GGYRCC 2 cut(s) 217, 347
BssECI CCNNGG 3 cut(s) 48, 60, 351
BssMI GATC 3 cut(s) 73, 137, 248
BssNI GRCGYC 1 cut(s) 218
BssT1I CCWWGG 1 cut(s) 48
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 3 cut(s) 76, 140, 251
BstMBI GATC 3 cut(s) 73, 137, 248
BstMCI CGRYCG 1 cut(s) 314
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 29, 50
BstSCI CCNGG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BsuRI GGCC 4 cut(s) 5, 23, 184, 305
Cac8I GCNNGC 1 cut(s) 25
CfoI GCGC 1 cut(s) 220
Cfr13I GGNCC 5 cut(s) 21, 182, 183, 255, 303
CseI GACGC 1 cut(s) 33
Csp6I GTAC 3 cut(s) 78, 145, 348
CviQI GTAC 3 cut(s) 78, 145, 348
DdeI CTNAG 1 cut(s) 323
DinI GGCGCC 1 cut(s) 219
DpnI GATC 3 cut(s) 75, 139, 250
DpnII GATC 3 cut(s) 73, 137, 248
DraI TTTAAA 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 135, 186
Eco47I GGWCC 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 2 cut(s) 135, 186
EgeI GGCGCC 1 cut(s) 219
EheI GGCGCC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 48
FaiI YATR 1 cut(s) 200
Fnu4HI GCNGC 2 cut(s) 6, 231
FriOI GRGCYC 2 cut(s) 135, 186
Fsp4HI GCNGC 2 cut(s) 6, 231
GlaI GCGC 1 cut(s) 219
GluI GCNGC 2 cut(s) 6, 231
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 4 cut(s) 5, 23, 184, 305
HapII CCGG 4 cut(s) 88, 100, 221, 262
HgaI GACGC 1 cut(s) 33
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 1 cut(s) 218
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HpaII CCGG 4 cut(s) 88, 100, 221, 262
HphI GGTGA 1 cut(s) 223
Hpy188I TCNGA 3 cut(s) 130, 142, 326
Hpy188III TCNNGA 3 cut(s) 63, 246, 262
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 3 cut(s) 202, 220, 338
HpyCH4V TGCA 1 cut(s) 271
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 29, 50
HpyF3I CTNAG 1 cut(s) 323
Hsp92I GRCGYC 1 cut(s) 218
HspAI GCGC 1 cut(s) 218
KasI GGCGCC 1 cut(s) 217
Kpn2I TCCGGA 1 cut(s) 261
KpnI GGTACC 1 cut(s) 351
Kzo9I GATC 3 cut(s) 73, 137, 248
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 8 cut(s) 4, 37, 101, 113, 148, 234, 237, 275
Lsp1109I GCAGC 1 cut(s) 217
MalI GATC 3 cut(s) 75, 139, 250
MboI GATC 3 cut(s) 73, 137, 248
MboII GAAGA 4 cut(s) 48, 214, 217, 310
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 2 cut(s) 135, 186
MluCI AATT 1 cut(s) 190
Mly113I GGCGCC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 7 cut(s) 38, 44, 70, 157, 196, 294, 346
MroI TCCGGA 1 cut(s) 261
MseI TTAA 1 cut(s) 318
MspI CCGG 4 cut(s) 88, 100, 221, 262
MspR9I CCNGG 1 cut(s) 101
MssI GTTTAAAC 1 cut(s) 319
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 29, 50
NarI GGCGCC 1 cut(s) 218
NciI CCSGG 1 cut(s) 101
NdeII GATC 3 cut(s) 73, 137, 248
NlaIV GGNNCC 5 cut(s) 132, 184, 219, 256, 349
PkrI GCNGC 2 cut(s) 7, 232
PluTI GGCGCC 1 cut(s) 221
PmeI GTTTAAAC 1 cut(s) 319
PspN4I GGNNCC 5 cut(s) 132, 184, 219, 256, 349
PspOMI GGGCCC 1 cut(s) 182
PspPI GGNCC 5 cut(s) 21, 182, 183, 255, 303
PsuI RGATCY 1 cut(s) 248
RsaI GTAC 3 cut(s) 79, 146, 349
RsaNI GTAC 3 cut(s) 78, 145, 348
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 2 cut(s) 6, 231
Sau3AI GATC 3 cut(s) 73, 137, 248
Sau96I GGNCC 5 cut(s) 21, 182, 183, 255, 303
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 2 cut(s) 135, 186
SetI ASST 3 cut(s) 213, 349, 357
SfoI GGCGCC 1 cut(s) 219
SinI GGWCC 1 cut(s) 255
Sse9I AATT 1 cut(s) 190
SsiI CCGC 1 cut(s) 6
SspDI GGCGCC 1 cut(s) 217
StyD4I CCNGG 1 cut(s) 99
StyI CCWWGG 1 cut(s) 48
TaqI TCGA 1 cut(s) 245
TaqII GACCGA 1 cut(s) 300
TasI AATT 1 cut(s) 190
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 1 cut(s) 215
TspGWI ACGGA 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.