Prupe.5G001600_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012234
Physical Location & Seq
Reverse (-)
290465 .. 291133
669 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G001600.1

Sequence Viewer

Length: 669 bp
ATGGGTGGATGCGTGTCGTCTGTGATCAGGAGTAGTCATAATGAAGAGCTTCTGCTCAATTCGCCAACCGCCAAAGTTATCTCCATCAATGGAAGTCTCCGCGAATACCCAGTTCCCGTGATTGTCTCCCAAGTCCTCGAGGCTGGCCAAACGGCATCCTCTTCGTCTTCGTCGTCGTCGTCGTTTTTATGCAATTCAGACCGTTTGTATTACGACAACTACATCCCGGTTTTGGATTCGGAAGATGAACTTGAGGCCGATCAGATCTACTTCATACTCCCGAGATCGAAGCTCGAACACCGCCTCAGCGCCACCGATATGGCGGCCTTGGCTGTGAGAGCCAGCCTCGCCTTCCAAGATGCCTCTTCCTCTTCTTCTAGTTACAAGACCAAGGAGAAGAAAAAGGACCTTCATCCTCGCCGTAATTACAAGAAGGCCCGCGTTTCTCCCGTTCTGATTAATTATGCAAACTCCGACATGGACAGAGATGACTTCAATGAAATTACCATCGGAGACTCGGCCTACAAAGGGCAGATGAGCCAGAAGCAGCAGATTTCGAGATCTAAGTCGGTTAAGAAATTGCAGAGATACACATCCAAAAGGGCCAAGATGGCCGTTCGATCTTTCAGACTCAGATTGACGACCATAGACGAAGGCTCTGTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

223

Amino Acids

24.76

Weight (kDa)

9.33

Isoelectric Point (pI)

62.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 137
AccII CGCG 2 cut(s) 102, 441
AciI CCGC 5 cut(s) 69, 100, 301, 323, 439
AcoI YGGCCR 2 cut(s) 145, 612
AfiI CCNNNNNNNGG 2 cut(s) 232, 528
AgsI TTSAA 1 cut(s) 496
AluBI AGCT 2 cut(s) 49, 292
AluI AGCT 2 cut(s) 49, 292
Alw26I GTCTC 3 cut(s) 101, 130, 507
Ama87I CYCGRG 2 cut(s) 137, 280
AoxI GGCC 7 cut(s) 145, 255, 324, 435, 519, 603, 612
ApeKI GCWGC 1 cut(s) 547
ArsI GACNNNNNNTTYG 1 cut(s) 645
AseI ATTAAT 1 cut(s) 459
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 3 cut(s) 406, 436, 603
AsuC2I CCSGG 1 cut(s) 227
AvaI CYCGRG 2 cut(s) 137, 280
AvaII GGWCC 1 cut(s) 406
BalI TGGCCA 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 159
BbvCI CCTCAGC 1 cut(s) 305
BbvI GCAGC 1 cut(s) 559
BccI CCATC 3 cut(s) 92, 515, 604
BceAI ACGGC 3 cut(s) 168, 405, 599
BcgI CGANNNNNNTGC 2 cut(s) 144, 178
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 101, 130, 507
BfaI CTAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 312
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 2 cut(s) 264, 560
BisI GCNGC 2 cut(s) 324, 548
BlsI GCNGC 2 cut(s) 325, 549
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 406
BmeT110I CYCGRG 2 cut(s) 137, 280
BmgT120I GGNCC 3 cut(s) 406, 436, 603
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 2 cut(s) 164, 349
BmuI ACTGGG 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 330, 362
Bpu10I CCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 327, 390
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 528
Bse1I ACTGG 1 cut(s) 110
Bse8I GATNNNNATC 1 cut(s) 592
BseDI CCNNGG 2 cut(s) 327, 390
BseGI GGATG 5 cut(s) 14, 155, 222, 412, 593
BseJI GATNNNNATC 1 cut(s) 592
BseLI CCNNNNNNNGG 2 cut(s) 232, 528
BseMII CTCAG 2 cut(s) 319, 646
BseNI ACTGG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 102, 441
BshFI GGCC 7 cut(s) 147, 257, 326, 437, 521, 605, 614
BsiHKCI CYCGRG 2 cut(s) 137, 280
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 2 cut(s) 232, 528
BsmAI GTCTC 3 cut(s) 101, 130, 507
BsnI GGCC 7 cut(s) 147, 257, 326, 437, 521, 605, 614
BsoBI CYCGRG 2 cut(s) 137, 280
Bsp143I GATC 6 cut(s) 24, 259, 264, 284, 560, 620
BspACI CCGC 5 cut(s) 69, 100, 301, 323, 439
BspANI GGCC 7 cut(s) 147, 257, 326, 437, 521, 605, 614
BspCNI CTCAG 2 cut(s) 318, 645
BspFNI CGCG 2 cut(s) 102, 441
BspQI GCTCTTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 327, 390
BssMI GATC 6 cut(s) 24, 259, 264, 284, 560, 620
BssT1I CCWWGG 2 cut(s) 327, 390
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 4 cut(s) 39, 166, 370, 376
BstC8I GCNNGC 3 cut(s) 145, 343, 439
BstDEI CTNAG 3 cut(s) 305, 564, 632
BstF5I GGATG 5 cut(s) 14, 155, 222, 412, 593
BstFNI CGCG 2 cut(s) 102, 441
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 6 cut(s) 27, 262, 267, 287, 563, 623
BstMAI GTCTC 3 cut(s) 101, 130, 507
BstMBI GATC 6 cut(s) 24, 259, 264, 284, 560, 620
BstMWI GCNNNNNNNGC 5 cut(s) 61, 329, 338, 347, 611
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 2 cut(s) 102, 441
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 1 cut(s) 159
BstX2I RGATCY 2 cut(s) 264, 560
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 2 cut(s) 264, 560
BsuRI GGCC 7 cut(s) 147, 257, 326, 437, 521, 605, 614
BtsCI GGATG 5 cut(s) 14, 155, 222, 412, 593
Cac8I GCNNGC 3 cut(s) 145, 343, 439
CfoI GCGC 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 406, 436, 603
CviAII CATG 1 cut(s) 478
DdeI CTNAG 3 cut(s) 305, 564, 632
DpnI GATC 6 cut(s) 26, 261, 266, 286, 562, 622
DpnII GATC 6 cut(s) 24, 259, 264, 284, 560, 620
EaeI YGGCCR 2 cut(s) 145, 612
Eam1104I CTCTTC 4 cut(s) 39, 166, 370, 376
EarI CTCTTC 4 cut(s) 39, 166, 370, 376
Eco130I CCWWGG 2 cut(s) 327, 390
Eco47I GGWCC 1 cut(s) 406
Eco88I CYCGRG 2 cut(s) 137, 280
EcoO109I RGGNCCY 1 cut(s) 406
EcoT14I CCWWGG 2 cut(s) 327, 390
ErhI CCWWGG 2 cut(s) 327, 390
FaeI CATG 1 cut(s) 481
FaiI YATR 7 cut(s) 39, 190, 275, 320, 465, 479, 647
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FatI CATG 1 cut(s) 477
FauI CCCGC 1 cut(s) 446
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 324, 548
FokI GGATG 5 cut(s) 21, 142, 209, 399, 580
Fsp4HI GCNGC 2 cut(s) 324, 548
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 310
GluI GCNGC 2 cut(s) 324, 548
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 7 cut(s) 147, 257, 326, 437, 521, 605, 614
HapII CCGG 1 cut(s) 227
HhaI GCGC 1 cut(s) 311
Hin1II CATG 1 cut(s) 481
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 3 cut(s) 236, 515, 630
HpaII CCGG 1 cut(s) 227
Hpy188I TCNGA 9 cut(s) 199, 241, 264, 456, 475, 512, 629, 635, 668
Hpy188III TCNNGA 3 cut(s) 28, 280, 558
Hpy99I CGWCG 4 cut(s) 175, 178, 181, 184
HpyAV CCTTC 4 cut(s) 361, 419, 427, 647
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4V TGCA 3 cut(s) 192, 467, 583
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 329, 338, 347, 611
HpyF3I CTNAG 3 cut(s) 305, 564, 632
Hsp92II CATG 1 cut(s) 481
HspAI GCGC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 6 cut(s) 24, 259, 264, 284, 560, 620
LguI GCTCTTC 1 cut(s) 39
LpnPI CCDG 6 cut(s) 13, 123, 129, 240, 355, 554
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 2 cut(s) 164, 349
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 380
MalI GATC 6 cut(s) 26, 261, 266, 286, 562, 622
MboI GATC 6 cut(s) 24, 259, 264, 284, 560, 620
MboII GAAGA 8 cut(s) 56, 153, 159, 254, 357, 363, 366, 409
MflI RGATCY 2 cut(s) 264, 560
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 6 cut(s) 58, 193, 424, 460, 501, 578
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 2 cut(s) 509, 624
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 9 cut(s) 133, 146, 169, 247, 314, 356, 373, 379, 426
Mox20I TGGCCA 1 cut(s) 147
MroXI GAANNNNTTC 1 cut(s) 48
MscI TGGCCA 1 cut(s) 147
MseI TTAA 2 cut(s) 459, 573
MslI CAYNNNNRTG 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 147
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 1 cut(s) 227
MvnI CGCG 2 cut(s) 102, 441
MwoI GCNNNNNNNGC 5 cut(s) 61, 329, 338, 347, 611
NciI CCSGG 1 cut(s) 227
NdeII GATC 6 cut(s) 24, 259, 264, 284, 560, 620
NlaIII CATG 1 cut(s) 481
NmeAIII GCCGAG 1 cut(s) 497
PaeR7I CTCGAG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 3 cut(s) 170, 176, 179
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 325, 549
PleI GAGTC 2 cut(s) 509, 624
PpsI GAGTC 2 cut(s) 509, 624
PpuMI RGGWCCY 1 cut(s) 406
PshBI ATTAAT 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 406
PspPI GGNCC 3 cut(s) 406, 436, 603
PspPPI RGGWCCY 1 cut(s) 406
PspXI VCTCGAGB 1 cut(s) 137
PsuI RGATCY 2 cut(s) 264, 560
RseI CAYNNNNRTG 1 cut(s) 317
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 459, 573
SatI GCNGC 2 cut(s) 324, 548
Sau3AI GATC 6 cut(s) 24, 259, 264, 284, 560, 620
Sau96I GGNCC 3 cut(s) 406, 436, 603
SchI GAGTC 2 cut(s) 509, 624
ScrFI CCNGG 1 cut(s) 227
SetI ASST 3 cut(s) 51, 294, 411
SfaNI GCATC 2 cut(s) 164, 349
SfiI GGCCNNNNNGGCC 1 cut(s) 611
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 406
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 137, 251
SmoI CTYRAG 2 cut(s) 137, 251
Sse9I AATT 6 cut(s) 58, 193, 424, 460, 501, 578
SsiI CCGC 5 cut(s) 69, 100, 301, 323, 439
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 2 cut(s) 327, 390
TaaI ACNGT 1 cut(s) 203
TaqI TCGA 5 cut(s) 138, 287, 294, 557, 619
TasI AATT 6 cut(s) 58, 193, 424, 460, 501, 578
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 2 cut(s) 459, 573
Tru9I TTAA 2 cut(s) 459, 573
TseI GCWGC 1 cut(s) 547
TspDTI ATGAA 5 cut(s) 57, 261, 262, 401, 513
VpaK11BI GGWCC 1 cut(s) 406
VspI ATTAAT 1 cut(s) 459
XhoI CTCGAG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.