FvH4_5g26600

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012234
Physical Location & Seq
Forward (+)
18048280 .. 18049657
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26600.t1

Sequence Viewer

Length: 669 bp
ATGGGTGGATGCTTCTCCGTCAATGCTAATTTTGAACCTTCCCTTCATTCTTCTTCTCCCACAGCCAAGGTTATCTCCGTGAACGGAAGTCTTCTTGAATACCCAGTTCCCATCGCCGTCTCGCAAGTGCTCAAGCCCGAGGAGGCTGCTTCTTCTTCCTCCACGCCCTCTTTTTTCGTCTGCAACTCCGACCGGCTTAACTACGACGATTATATTCCTGCTTTGGAATCCCACCACCAACTCGAGGCTGATCAGATCTACTTCGTTCTCCCGGTCGCTAAGCTCCAACGTCGCCTCACTGCCTCGGATATGGCCGCGTTCGCCGTCAGGGCCAGCCTCGCCCTCCAGAATGCATCCGCGGCTTCTTCCTCATCGGAAAAGAAGGATCGACTTCGTCGACGGAAACAGAACAGGGTTTCTCCTATTTTCGTTGTCAAGTCGGAGCCCACCAATTTTGTGGATTACGAGGTTGATTTTACGATTGGTCCCTCCAATTCCCTTATGAAGAAGAAGGTGATGGCGCCGGAGAAGCCTGCAGGTTTGGGGGTTTCCAGATCTGGGTCCGTCAGGAAGTTGCAGAGATACACATCTAAAAGGGTGTTGAAGAGAGCCGTTCGGTCGTTTAAACTCAGATTGGCCCCAATACAAGAAGGTACCGAGGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

223

Amino Acids

24.44

Weight (kDa)

9.8

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 217 3.5e-28 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 527
Acc65I GGTACC 1 cut(s) 653
AccB1I GGYRCC 2 cut(s) 520, 653
AccI GTMKAC 1 cut(s) 397
AccII CGCG 2 cut(s) 317, 359
AciI CCGC 3 cut(s) 315, 357, 359
AclWI GGATC 1 cut(s) 393
AcoI YGGCCR 1 cut(s) 312
AcyI GRCGYC 1 cut(s) 521
AfaI GTAC 1 cut(s) 655
AfiI CCNNNNNNNGG 2 cut(s) 244, 558
AgsI TTSAA 3 cut(s) 35, 98, 604
AluBI AGCT 1 cut(s) 283
AluI AGCT 1 cut(s) 283
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 1 cut(s) 393
Ama87I CYCGRG 2 cut(s) 137, 242
AoxI GGCC 3 cut(s) 312, 330, 636
ApeKI GCWGC 1 cut(s) 146
Asp718I GGTACC 1 cut(s) 653
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 4 cut(s) 330, 485, 561, 637
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 1 cut(s) 526
AvaI CYCGRG 2 cut(s) 137, 242
AvaII GGWCC 2 cut(s) 485, 561
BaeI ACNNNNGTAYC 1 cut(s) 645
BanI GGYRCC 2 cut(s) 520, 653
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 133
BccI CCATC 2 cut(s) 119, 511
BceAI ACGGC 3 cut(s) 101, 308, 596
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 124
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 527
BglI GCCNNNNNGGC 1 cut(s) 329
BglII AGATCT 2 cut(s) 255, 554
BisI GCNGC 3 cut(s) 147, 315, 360
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 3 cut(s) 148, 316, 361
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 485, 561
BmeT110I CYCGRG 2 cut(s) 137, 242
BmgT120I GGNCC 4 cut(s) 330, 485, 561, 637
BmiI GGNNCC 6 cut(s) 444, 487, 522, 562, 639, 655
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 362
BmuI ACTGGG 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 329
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 272
BsaBI GATNNNNATC 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 521
BsaJI CCNNGG 5 cut(s) 66, 138, 303, 357, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 558
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 1 cut(s) 104
Bse8I GATNNNNATC 1 cut(s) 586
BseDI CCNNGG 5 cut(s) 66, 138, 303, 357, 657
BseGI GGATG 2 cut(s) 14, 353
BseJI GATNNNNATC 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 244, 558
BseMII CTCAG 1 cut(s) 643
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 133
Bsh1236I CGCG 2 cut(s) 317, 359
Bsh1285I CGRYCG 3 cut(s) 193, 276, 620
BshFI GGCC 3 cut(s) 314, 332, 638
BshNI GGYRCC 2 cut(s) 520, 653
BsiEI CGRYCG 3 cut(s) 193, 276, 620
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 2 cut(s) 137, 242
BsiSI CCGG 3 cut(s) 193, 272, 524
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 2 cut(s) 244, 558
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 3 cut(s) 314, 332, 638
BsoBI CYCGRG 2 cut(s) 137, 242
Bsp1286I GDGCHC 2 cut(s) 132, 447
Bsp143I GATC 4 cut(s) 250, 255, 385, 554
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 3 cut(s) 315, 357, 359
BspANI GGCC 3 cut(s) 314, 332, 638
BspCNI CTCAG 1 cut(s) 642
BspFNI CGCG 2 cut(s) 317, 359
BspLI GGNNCC 6 cut(s) 444, 487, 522, 562, 639, 655
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 1 cut(s) 527
BspPI GGATC 1 cut(s) 393
BspT107I GGYRCC 2 cut(s) 520, 653
BsrFI RCCGGY 1 cut(s) 192
BsrI ACTGG 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 5 cut(s) 66, 138, 303, 357, 657
BssMI GATC 4 cut(s) 250, 255, 385, 554
BssNI GRCGYC 1 cut(s) 521
BssT1I CCWWGG 1 cut(s) 66
Bst6I CTCTTC 1 cut(s) 599
BstACI GRCGYC 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 334, 534
BstDEI CTNAG 2 cut(s) 279, 629
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 2 cut(s) 14, 353
BstFNI CGCG 2 cut(s) 317, 359
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 4 cut(s) 253, 258, 388, 557
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 4 cut(s) 250, 255, 385, 554
BstMCI CGRYCG 3 cut(s) 193, 276, 620
BstMWI GCNNNNNNNGC 5 cut(s) 320, 329, 338, 359, 529
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 534
BstUI CGCG 2 cut(s) 317, 359
BstV1I GCAGC 1 cut(s) 133
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 2 cut(s) 255, 554
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 2 cut(s) 255, 554
BsuRI GGCC 3 cut(s) 314, 332, 638
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 14, 353
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 2 cut(s) 334, 534
CfoI GCGC 1 cut(s) 523
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 4 cut(s) 330, 485, 561, 637
Cfr42I CCGCGG 1 cut(s) 360
Csp6I GTAC 1 cut(s) 654
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviQI GTAC 1 cut(s) 654
DdeI CTNAG 2 cut(s) 279, 629
DinI GGCGCC 1 cut(s) 522
DpnI GATC 4 cut(s) 252, 257, 387, 556
DpnII GATC 4 cut(s) 250, 255, 385, 554
DraI TTTAAA 1 cut(s) 625
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 447
Eco47I GGWCC 2 cut(s) 485, 561
Eco88I CYCGRG 2 cut(s) 137, 242
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 447
EgeI GGCGCC 1 cut(s) 522
EheI GGCGCC 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 124
FaiI YATR 3 cut(s) 213, 311, 503
FaqI GGGAC 1 cut(s) 471
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 3 cut(s) 147, 315, 360
FokI GGATG 2 cut(s) 21, 340
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 3 cut(s) 147, 315, 360
GlaI GCGC 1 cut(s) 522
GluI GCNGC 3 cut(s) 147, 315, 360
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 3 cut(s) 314, 332, 638
HapII CCGG 3 cut(s) 193, 272, 524
HhaI GCGC 1 cut(s) 523
Hin1I GRCGYC 1 cut(s) 521
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HincII GTYRAC 1 cut(s) 398
HindII GTYRAC 1 cut(s) 398
HinfI GANTC 1 cut(s) 227
HpaII CCGG 3 cut(s) 193, 272, 524
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 2 cut(s) 82, 398
Hpy188I TCNGA 6 cut(s) 190, 255, 307, 376, 442, 632
Hpy188III TCNNGA 4 cut(s) 95, 346, 552, 568
Hpy8I GTNNAC 2 cut(s) 82, 398
Hpy99I CGWCG 4 cut(s) 209, 294, 399, 402
HpyAV CCTTC 5 cut(s) 48, 53, 376, 505, 644
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 4 cut(s) 183, 353, 536, 577
HpyF10VI GCNNNNNNNGC 5 cut(s) 320, 329, 338, 359, 529
HpyF3I CTNAG 2 cut(s) 279, 629
HpySE526I ACGT 1 cut(s) 289
Hsp92I GRCGYC 1 cut(s) 521
HspAI GCGC 1 cut(s) 521
KasI GGCGCC 1 cut(s) 520
KpnI GGTACC 1 cut(s) 657
Ksp22I TGATCA 1 cut(s) 250
KspI CCGCGG 1 cut(s) 360
Kzo9I GATC 4 cut(s) 250, 255, 385, 554
LmnI GCTCC 2 cut(s) 288, 442
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 1 cut(s) 362
MaeII ACGT 1 cut(s) 289
MalI GATC 4 cut(s) 252, 257, 387, 556
MboI GATC 4 cut(s) 250, 255, 385, 554
MboII GAAGA 9 cut(s) 42, 45, 83, 144, 147, 357, 517, 520, 616
MflI RGATCY 2 cut(s) 255, 554
MhlI GDGCHC 2 cut(s) 132, 447
MluCI AATT 3 cut(s) 28, 451, 493
Mly113I GGCGCC 1 cut(s) 521
MmeI TCCRAC 3 cut(s) 213, 310, 420
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 2 cut(s) 198, 624
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 3 cut(s) 193, 272, 524
MspR9I CCNGG 1 cut(s) 272
MssI GTTTAAAC 1 cut(s) 625
Mva1269I GAATGC 1 cut(s) 355
MvnI CGCG 2 cut(s) 317, 359
MwoI GCNNNNNNNGC 5 cut(s) 320, 329, 338, 359, 529
NarI GGCGCC 1 cut(s) 521
NciI CCSGG 1 cut(s) 272
NdeII GATC 4 cut(s) 250, 255, 385, 554
NlaIV GGNNCC 6 cut(s) 444, 487, 522, 562, 639, 655
NsiI ATGCAT 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 394
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 227
PflFI GACNNNGTC 1 cut(s) 393
PkrI GCNGC 3 cut(s) 148, 316, 361
PluTI GGCGCC 1 cut(s) 524
PmeI GTTTAAAC 1 cut(s) 625
PspN4I GGNNCC 6 cut(s) 444, 487, 522, 562, 639, 655
PspPI GGNCC 4 cut(s) 330, 485, 561, 637
PspXI VCTCGAGB 1 cut(s) 242
PstI CTGCAG 1 cut(s) 538
PsuI RGATCY 2 cut(s) 255, 554
PsyI GACNNNGTC 1 cut(s) 393
RsaI GTAC 1 cut(s) 655
RsaNI GTAC 1 cut(s) 654
SacII CCGCGG 1 cut(s) 360
SalI GTCGAC 1 cut(s) 396
SaqAI TTAA 2 cut(s) 198, 624
SatI GCNGC 3 cut(s) 147, 315, 360
Sau3AI GATC 4 cut(s) 250, 255, 385, 554
Sau96I GGNCC 4 cut(s) 330, 485, 561, 637
SbfI CCTGCAGG 1 cut(s) 538
ScrFI CCNGG 1 cut(s) 272
SdaI CCTGCAGG 1 cut(s) 538
SduI GDGCHC 2 cut(s) 132, 447
SetI ASST 9 cut(s) 40, 72, 285, 292, 471, 516, 541, 655, 663
SfaNI GCATC 1 cut(s) 362
SfcI CTRYAG 1 cut(s) 534
SfoI GGCGCC 1 cut(s) 522
Sfr274I CTCGAG 1 cut(s) 242
Sfr303I CCGCGG 1 cut(s) 360
SgrBI CCGCGG 1 cut(s) 360
SgrDI CGTCGACG 1 cut(s) 396
SinI GGWCC 2 cut(s) 485, 561
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 131, 242
SmoI CTYRAG 2 cut(s) 131, 242
Sse8387I CCTGCAGG 1 cut(s) 538
Sse9I AATT 3 cut(s) 28, 451, 493
SsiI CCGC 3 cut(s) 315, 357, 359
SspDI GGCGCC 1 cut(s) 520
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 66
TaiI ACGT 1 cut(s) 292
TaqI TCGA 3 cut(s) 243, 388, 397
TaqII GACCGA 1 cut(s) 606
TasI AATT 3 cut(s) 28, 451, 493
TauI GCSGC 2 cut(s) 317, 362
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 2 cut(s) 198, 624
Tru9I TTAA 2 cut(s) 198, 624
TscAI CASTG 1 cut(s) 304
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 35, 518
TspGWI ACGGA 5 cut(s) 7, 67, 99, 415, 553
TspRI CASTG 1 cut(s) 304
Tth111I GACNNNGTC 1 cut(s) 393
VpaK11BI GGWCC 2 cut(s) 485, 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 397
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.