MD07G1142400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012234
Physical Location & Seq
Reverse (-)
20645197 .. 20645925
729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1142400.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGGTGGATGCTTGTCTACAGTCATCGTCAGGAGGACTAGTTCAAGCGGAAGAGATGATGATGATTCGTCGTCCACTGCCAAAGTAATCTACATGAACGCATGTGTCCGCGAATACCCGGTTCCCCTTAATGTCTCCAAAGTCCTTGAGGCCGATCAATCGTCGTCTTCGTCTTCATCTTTGTTTATATGTCATTCCGACAGTTTGTATTACGACAGTTACATTCCGGCTTTAGATTCCGAAGATGAGCTGGAGGCCAATCAGATCTACTTCGTACTCCCCAGATCCAAGCTTCAACACCGCCTCAGTGCCACCGATATGGCGGCCCTCGCAGTCAGAGCCAGCCTCGCCCTCCAGAAAATTAATACCAATGCTTCTTCTTCTTCTTCCTCCTCCAAGAATGAAAAGGGAAACAAAAAGGAAAATAAGGGAAGCGGATACCGTAATTACAACAAGGCCCGCATTTCTCCGGTGATTGAGATGAATAATAATAAATATGCAAACTTGTCGGTCGAGGGCGACGGTGGTGATTTGAGCATGAATGGATTAACGATTGGAGGTGGAGGGTCATTAGCGTACAAAGGCCAGAAGATGATGAGTAGTAGCGGCCGCGGCGGTGGGTCATCGTCGGTTAGGAAATTGCGGAGATATACATCGAGAAAGGCAAAGTTGGCCACTCGTTCTTTTAGGCTCAGATTGTCAACCATTCACGAAGGCTCCGTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

243

Amino Acids

26.11

Weight (kDa)

9.59

Isoelectric Point (pI)

65.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 238 1.5e-30 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 17
AccII CGCG 2 cut(s) 111, 612
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 2 cut(s) 607, 672
AfaI GTAC 2 cut(s) 276, 578
AgsI TTSAA 2 cut(s) 45, 296
AhlI ACTAGT 1 cut(s) 38
AloI GAACNNNNNNTCC 2 cut(s) 25, 57
AluBI AGCT 2 cut(s) 250, 292
AluI AGCT 2 cut(s) 250, 292
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 279
AoxI GGCC 7 cut(s) 150, 255, 324, 456, 583, 607, 672
ArsI GACNNNNNNTTYG 1 cut(s) 705
AseI ATTAAT 1 cut(s) 363
AspS9I GGNCC 2 cut(s) 325, 457
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 484, 539
BalI TGGCCA 1 cut(s) 674
BbsI GAAGAC 2 cut(s) 159, 165
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciVI GTATCC 1 cut(s) 431
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 1 cut(s) 39
BfmI CTRYAG 1 cut(s) 18
BfuI GTATCC 1 cut(s) 431
BglII AGATCT 1 cut(s) 264
BisI GCNGC 4 cut(s) 324, 607, 610, 613
BlsI GCNGC 4 cut(s) 325, 608, 611, 614
Bme1390I CCNGG 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 325, 457
BmiI GGNNCC 2 cut(s) 123, 718
BmrFI CCNGG 1 cut(s) 119
BpiI GAAGAC 2 cut(s) 159, 165
BplI GAGNNNNNCTC 2 cut(s) 330, 362
BpmI CTGGAG 2 cut(s) 272, 338
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 652
BsaJI CCNNGG 1 cut(s) 610
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 3 cut(s) 25, 55, 701
Bse8I GATNNNNATC 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 610
BseGI GGATG 1 cut(s) 14
BseJI GATNNNNATC 1 cut(s) 652
BseMII CTCAG 2 cut(s) 319, 706
BseRI GAGGAG 1 cut(s) 382
BseX3I CGGCCG 1 cut(s) 607
Bsh1236I CGCG 2 cut(s) 111, 612
Bsh1285I CGRYCG 2 cut(s) 513, 610
BshFI GGCC 7 cut(s) 152, 257, 326, 458, 585, 609, 674
BsiEI CGRYCG 2 cut(s) 513, 610
BsiSI CCGG 3 cut(s) 119, 227, 470
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 7 cut(s) 152, 257, 326, 458, 585, 609, 674
Bsp143I GATC 3 cut(s) 154, 264, 284
BspANI GGCC 7 cut(s) 152, 257, 326, 458, 585, 609, 674
BspCNI CTCAG 2 cut(s) 318, 705
BspFNI CGCG 2 cut(s) 111, 612
BspLI GGNNCC 2 cut(s) 123, 718
BspPI GGATC 1 cut(s) 279
BssECI CCNNGG 1 cut(s) 610
BssMI GATC 3 cut(s) 154, 264, 284
Bst4CI ACNGT 5 cut(s) 22, 203, 218, 443, 524
Bst6I CTCTTC 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 343, 460
BstDEI CTNAG 2 cut(s) 305, 692
BstDSI CCRYGG 1 cut(s) 610
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 2 cut(s) 111, 612
BstKTI GATC 3 cut(s) 157, 267, 287
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 3 cut(s) 154, 264, 284
BstMCI CGRYCG 2 cut(s) 513, 610
BstMWI GCNNNNNNNGC 5 cut(s) 329, 338, 347, 612, 671
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 1 cut(s) 18
BstUI CGCG 2 cut(s) 111, 612
BstV2I GAAGAC 2 cut(s) 159, 165
BstX2I RGATCY 2 cut(s) 264, 284
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 2 cut(s) 264, 284
BstZI CGGCCG 1 cut(s) 607
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 7 cut(s) 152, 257, 326, 458, 585, 609, 674
BtgI CCRYGG 1 cut(s) 610
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 2 cut(s) 75, 313
Cac8I GCNNGC 2 cut(s) 343, 460
CciNI GCGGCCGC 1 cut(s) 607
Cfr13I GGNCC 2 cut(s) 325, 457
Cfr42I CCGCGG 1 cut(s) 613
Csp6I GTAC 2 cut(s) 275, 577
CviAII CATG 3 cut(s) 94, 102, 538
CviQI GTAC 2 cut(s) 275, 577
DdeI CTNAG 2 cut(s) 305, 692
DpnI GATC 3 cut(s) 156, 266, 286
DpnII GATC 3 cut(s) 154, 264, 284
EaeI YGGCCR 2 cut(s) 607, 672
EagI CGGCCG 1 cut(s) 607
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 607
Eco52I CGGCCG 1 cut(s) 607
FaeI CATG 3 cut(s) 97, 105, 541
FaiI YATR 8 cut(s) 95, 103, 188, 190, 320, 498, 539, 651
FatI CATG 3 cut(s) 93, 101, 537
FauI CCCGC 1 cut(s) 467
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 4 cut(s) 324, 607, 610, 613
FokI GGATG 1 cut(s) 21
Fsp4HI GCNGC 4 cut(s) 324, 607, 610, 613
FspBI CTAG 1 cut(s) 39
GluI GCNGC 4 cut(s) 324, 607, 610, 613
GsuI CTGGAG 2 cut(s) 272, 338
HaeIII GGCC 7 cut(s) 152, 257, 326, 458, 585, 609, 674
HapII CCGG 3 cut(s) 119, 227, 470
Hin1II CATG 3 cut(s) 97, 105, 541
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 65, 236
HpaII CCGG 3 cut(s) 119, 227, 470
HphI GGTGA 2 cut(s) 484, 539
Hpy166II GTNNAC 3 cut(s) 18, 75, 702
Hpy188I TCNGA 6 cut(s) 199, 241, 264, 338, 695, 728
Hpy188III TCNNGA 4 cut(s) 31, 355, 657, 710
Hpy8I GTNNAC 3 cut(s) 18, 75, 702
Hpy99I CGWCG 4 cut(s) 73, 166, 524, 631
HpyAV CCTTC 1 cut(s) 707
HpyCH4III ACNGT 5 cut(s) 22, 203, 218, 443, 524
HpyCH4V TGCA 1 cut(s) 500
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 338, 347, 612, 671
HpyF3I CTNAG 2 cut(s) 305, 692
Hsp92II CATG 3 cut(s) 97, 105, 541
KspI CCGCGG 1 cut(s) 613
Kzo9I GATC 3 cut(s) 154, 264, 284
LmnI GCTCC 1 cut(s) 722
LpnPI CCDG 9 cut(s) 16, 132, 236, 240, 295, 355, 368, 483, 599
MaeI CTAG 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 218
MalI GATC 3 cut(s) 156, 266, 286
MboI GATC 3 cut(s) 154, 264, 284
MboII GAAGA 9 cut(s) 63, 159, 165, 254, 369, 372, 375, 378, 601
MflI RGATCY 2 cut(s) 264, 284
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 3 cut(s) 360, 445, 638
MluNI TGGCCA 1 cut(s) 674
MmeI TCCRAC 1 cut(s) 222
Mox20I TGGCCA 1 cut(s) 674
MscI TGGCCA 1 cut(s) 674
MseI TTAA 3 cut(s) 129, 363, 548
MslI CAYNNNNRTG 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 612
MspI CCGG 3 cut(s) 119, 227, 470
MspR9I CCNGG 1 cut(s) 119
MvnI CGCG 2 cut(s) 111, 612
MwoI GCNNNNNNNGC 5 cut(s) 329, 338, 347, 612, 671
NciI CCSGG 1 cut(s) 119
NdeII GATC 3 cut(s) 154, 264, 284
NlaIII CATG 3 cut(s) 97, 105, 541
NlaIV GGNNCC 2 cut(s) 123, 718
NotI GCGGCCGC 1 cut(s) 607
NspI RCATGY 1 cut(s) 105
PcsI WCGNNNNNNNCGW 2 cut(s) 167, 717
PfeI GAWTC 2 cut(s) 65, 236
PkrI GCNGC 4 cut(s) 325, 608, 611, 614
PshBI ATTAAT 1 cut(s) 363
PspN4I GGNNCC 2 cut(s) 123, 718
PspPI GGNCC 2 cut(s) 325, 457
PsuI RGATCY 2 cut(s) 264, 284
RsaI GTAC 2 cut(s) 276, 578
RsaNI GTAC 2 cut(s) 275, 577
RseI CAYNNNNRTG 1 cut(s) 317
SacII CCGCGG 1 cut(s) 613
SaqAI TTAA 3 cut(s) 129, 363, 548
SatI GCNGC 4 cut(s) 324, 607, 610, 613
Sau3AI GATC 3 cut(s) 154, 264, 284
Sau96I GGNCC 2 cut(s) 325, 457
ScrFI CCNGG 1 cut(s) 119
SetI ASST 3 cut(s) 252, 294, 562
SfcI CTRYAG 1 cut(s) 18
Sfr303I CCGCGG 1 cut(s) 613
SgrBI CCGCGG 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SpeI ACTAGT 1 cut(s) 38
Sse9I AATT 3 cut(s) 360, 445, 638
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 117
TaaI ACNGT 5 cut(s) 22, 203, 218, 443, 524
TaqI TCGA 2 cut(s) 513, 656
TaqII GACCGA 1 cut(s) 499
TasI AATT 3 cut(s) 360, 445, 638
TauI GCSGC 4 cut(s) 326, 609, 612, 615
TfiI GAWTC 2 cut(s) 65, 236
Tru1I TTAA 3 cut(s) 129, 363, 548
Tru9I TTAA 3 cut(s) 129, 363, 548
TscAI CASTG 2 cut(s) 82, 313
TspDTI ATGAA 5 cut(s) 110, 165, 417, 497, 554
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 2 cut(s) 82, 313
VspI ATTAAT 1 cut(s) 363
XceI RCATGY 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.