Rh7BG300200

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012234
Physical Location & Seq
Forward (+)
28521692 .. 28522893
1202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG300200.1

Sequence Viewer

Length: 672 bp
ATGGGTGGATGCTTCTCCGTGAGCGCTTTTGAACCTTCTCTTCATTCTTCGTCTCCCACCGCCAAAGTTGTCTCCGCGAACGGAACTCTCATAGAATACCCTGTTCCCGTTTCCGTCTCGCAAGTCCTCAAGGTCGAGAAGGCAGCTTCTCCCTCTTCCTCCACGGCGTCGTTTTTCGTCTGCAACTCCGACCGGTTTAACTACGACTCTTATATTCCTGCTTTGGATTCCGACCACCAACTCGAGGCTGATCAGATCTACTTCGTACTCCCGGTTGCTAAGCTCCAGCGGCGGCTGACTGCCTCGGATATGGCCGCTTTGGCTGTCAGGGCCAGCCTCGCCCTCCAAAATGCGTCCACGGCTTCTTCCTCGGGAAAAAAGGATCGTACTCGTCGCCGGAACAAGAACCGGGTTTCTCCCATTCTCGTTGTCAAGTCGGAGCCCAGATCGGAGTACGATTTTGTGGATTACGAGGCTGATTTTACGATTGGGCCCTCCAATTCTCTTATGAAGAAGAAGGTGATGGCGCCGGAGAAGGCTGCTTTGGGGGTTTCGAGATCTGGGTCCGTCCGGAAGTTGCAGAGATACACATCTAAAAGGGTATTGAAGAGGGCCGTTCGGTCGTTTAAACTCAGATTGACCCCAATACAAGAAGGTACCGAGGTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

223

Amino Acids

24.45

Weight (kDa)

9.89

Isoelectric Point (pI)

59.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 218 3.2e-30 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 2 cut(s) 526, 656
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 570
AciI CCGC 5 cut(s) 60, 75, 289, 292, 315
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 312
AcyI GRCGYC 2 cut(s) 167, 527
AfaI GTAC 4 cut(s) 267, 388, 455, 658
AfeI AGCGCT 1 cut(s) 25
AfiI CCNNNNNNNGG 1 cut(s) 244
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 2 cut(s) 32, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AluBI AGCT 2 cut(s) 146, 283
AluI AGCT 2 cut(s) 146, 283
Alw26I GTCTC 3 cut(s) 57, 76, 121
AlwI GGATC 1 cut(s) 390
Ama87I CYCGRG 2 cut(s) 242, 370
Aor13HI TCCGGA 1 cut(s) 570
Aor51HI AGCGCT 1 cut(s) 25
AoxI GGCC 4 cut(s) 312, 330, 491, 612
ApaI GGGCCC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 143, 539
AsiGI ACCGGT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 2 cut(s) 26, 529
AspS9I GGNCC 5 cut(s) 330, 491, 492, 564, 612
AsuC2I CCSGG 2 cut(s) 272, 410
AsuHPI GGTGA 1 cut(s) 532
AvaI CYCGRG 2 cut(s) 242, 370
AvaII GGWCC 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 495
BaeI ACNNNNGTAYC 1 cut(s) 648
BanI GGYRCC 2 cut(s) 526, 656
BanII GRGCYC 2 cut(s) 444, 495
BbvI GCAGC 2 cut(s) 155, 526
BccI CCATC 1 cut(s) 517
BceAI ACGGC 3 cut(s) 180, 375, 599
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 2 cut(s) 272, 410
BcoDI GTCTC 3 cut(s) 57, 76, 121
BfoI RGCGCY 2 cut(s) 27, 530
BglI GCCNNNNNGGC 1 cut(s) 320
BglII AGATCT 2 cut(s) 255, 557
BisI GCNGC 5 cut(s) 144, 290, 293, 315, 540
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 5 cut(s) 145, 291, 294, 316, 541
Bme1390I CCNGG 2 cut(s) 272, 410
Bme18I GGWCC 1 cut(s) 564
BmeT110I CYCGRG 2 cut(s) 242, 370
BmgT120I GGNCC 5 cut(s) 330, 491, 492, 564, 612
BmiI GGNNCC 5 cut(s) 441, 493, 528, 565, 658
BmrFI CCNGG 2 cut(s) 272, 410
BpmI CTGGAG 1 cut(s) 269
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 2 cut(s) 272, 410
BsaBI GATNNNNATC 1 cut(s) 589
BsaHI GRCGYC 2 cut(s) 167, 527
BsaJI CCNNGG 5 cut(s) 162, 303, 357, 369, 660
BsaWI WCCGGW 2 cut(s) 192, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse118I RCCGGY 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 589
BseAI TCCGGA 1 cut(s) 570
BseDI CCNNGG 5 cut(s) 162, 303, 357, 369, 660
BseGI GGATG 1 cut(s) 14
BseJI GATNNNNATC 1 cut(s) 589
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 646
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 2 cut(s) 155, 526
Bsh1236I CGCG 1 cut(s) 77
Bsh1285I CGRYCG 2 cut(s) 193, 623
BshFI GGCC 4 cut(s) 314, 332, 493, 614
BshNI GGYRCC 2 cut(s) 526, 656
BshTI ACCGGT 1 cut(s) 192
BsiEI CGRYCG 2 cut(s) 193, 623
BsiHKCI CYCGRG 2 cut(s) 242, 370
BsiSI CCGG 6 cut(s) 193, 272, 397, 409, 530, 571
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 3 cut(s) 57, 76, 121
BsmBI CGTCTC 2 cut(s) 57, 121
BsnI GGCC 4 cut(s) 314, 332, 493, 614
BsoBI CYCGRG 2 cut(s) 242, 370
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 444, 495
Bsp13I TCCGGA 1 cut(s) 570
Bsp143I GATC 5 cut(s) 250, 255, 382, 446, 557
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 5 cut(s) 60, 75, 289, 292, 315
BspANI GGCC 4 cut(s) 314, 332, 493, 614
BspCNI CTCAG 1 cut(s) 645
BspEI TCCGGA 1 cut(s) 570
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 5 cut(s) 441, 493, 528, 565, 658
BspPI GGATC 1 cut(s) 390
BspT107I GGYRCC 2 cut(s) 526, 656
BsrFI RCCGGY 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 5 cut(s) 162, 303, 357, 369, 660
BssMI GATC 5 cut(s) 250, 255, 382, 446, 557
BssNI GRCGYC 2 cut(s) 167, 527
Bst6I CTCTTC 3 cut(s) 45, 160, 602
BstACI GRCGYC 2 cut(s) 167, 527
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 2 cut(s) 279, 632
BstDSI CCRYGG 2 cut(s) 162, 357
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 2 cut(s) 27, 530
BstHHI GCGC 2 cut(s) 26, 529
BstKTI GATC 5 cut(s) 253, 258, 385, 449, 560
BstMAI GTCTC 3 cut(s) 57, 76, 121
BstMBI GATC 5 cut(s) 250, 255, 382, 446, 557
BstMCI CGRYCG 2 cut(s) 193, 623
BstMWI GCNNNNNNNGC 5 cut(s) 289, 320, 329, 338, 359
BstSCI CCNGG 2 cut(s) 270, 408
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 155, 526
BstX2I RGATCY 2 cut(s) 255, 557
BstYI RGATCY 2 cut(s) 255, 557
BsuRI GGCC 4 cut(s) 314, 332, 493, 614
BtgI CCRYGG 2 cut(s) 162, 357
BtsCI GGATG 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 334
CfoI GCGC 2 cut(s) 26, 529
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 5 cut(s) 330, 491, 492, 564, 612
CseI GACGC 2 cut(s) 156, 342
Csp6I GTAC 4 cut(s) 266, 387, 454, 657
CspAI ACCGGT 1 cut(s) 192
CviQI GTAC 4 cut(s) 266, 387, 454, 657
DdeI CTNAG 2 cut(s) 279, 632
DinI GGCGCC 1 cut(s) 528
DpnI GATC 5 cut(s) 252, 257, 384, 448, 559
DpnII GATC 5 cut(s) 250, 255, 382, 446, 557
DraI TTTAAA 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 3 cut(s) 45, 160, 602
EarI CTCTTC 3 cut(s) 45, 160, 602
Eco24I GRGCYC 2 cut(s) 444, 495
Eco47I GGWCC 1 cut(s) 564
Eco47III AGCGCT 1 cut(s) 25
Eco88I CYCGRG 2 cut(s) 242, 370
EcoO109I RGGNCCY 1 cut(s) 492
EcoT38I GRGCYC 2 cut(s) 444, 495
EgeI GGCGCC 1 cut(s) 528
EheI GGCGCC 1 cut(s) 528
Esp3I CGTCTC 2 cut(s) 57, 121
FaiI YATR 4 cut(s) 92, 213, 311, 509
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 5 cut(s) 144, 290, 293, 315, 540
FokI GGATG 1 cut(s) 21
FriOI GRGCYC 2 cut(s) 444, 495
Fsp4HI GCNGC 5 cut(s) 144, 290, 293, 315, 540
GlaI GCGC 2 cut(s) 25, 528
GluI GCNGC 5 cut(s) 144, 290, 293, 315, 540
GsuI CTGGAG 1 cut(s) 269
HaeII RGCGCY 2 cut(s) 27, 530
HaeIII GGCC 4 cut(s) 314, 332, 493, 614
HapII CCGG 6 cut(s) 193, 272, 397, 409, 530, 571
HgaI GACGC 2 cut(s) 156, 342
HhaI GCGC 2 cut(s) 26, 529
Hin1I GRCGYC 2 cut(s) 167, 527
Hin6I GCGC 2 cut(s) 24, 527
HinP1I GCGC 2 cut(s) 24, 527
HinfI GANTC 2 cut(s) 206, 227
HpaII CCGG 6 cut(s) 193, 272, 397, 409, 530, 571
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 7 cut(s) 190, 232, 255, 307, 439, 451, 635
Hpy188III TCNNGA 4 cut(s) 136, 372, 555, 571
Hpy8I GTNNAC 1 cut(s) 357
Hpy99I CGWCG 2 cut(s) 172, 396
HpyAV CCTTC 5 cut(s) 45, 133, 511, 529, 647
HpyCH4V TGCA 2 cut(s) 183, 580
HpyF10VI GCNNNNNNNGC 5 cut(s) 289, 320, 329, 338, 359
HpyF3I CTNAG 2 cut(s) 279, 632
Hsp92I GRCGYC 2 cut(s) 167, 527
HspAI GCGC 2 cut(s) 24, 527
KasI GGCGCC 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 570
KpnI GGTACC 1 cut(s) 660
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 5 cut(s) 250, 255, 382, 446, 557
LmnI GCTCC 2 cut(s) 288, 439
Lsp1109I GCAGC 2 cut(s) 155, 526
MalI GATC 5 cut(s) 252, 257, 384, 448, 559
MboI GATC 5 cut(s) 250, 255, 382, 446, 557
MboII GAAGA 7 cut(s) 32, 39, 147, 357, 523, 526, 619
MflI RGATCY 2 cut(s) 255, 557
MhlI GDGCHC 2 cut(s) 444, 495
MluCI AATT 1 cut(s) 499
Mly113I GGCGCC 1 cut(s) 527
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 3 cut(s) 213, 255, 417
MroI TCCGGA 1 cut(s) 570
MseI TTAA 2 cut(s) 198, 627
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 6 cut(s) 193, 272, 397, 409, 530, 571
MspR9I CCNGG 2 cut(s) 272, 410
MssI GTTTAAAC 1 cut(s) 628
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 5 cut(s) 289, 320, 329, 338, 359
NarI GGCGCC 1 cut(s) 527
NciI CCSGG 2 cut(s) 272, 410
NdeII GATC 5 cut(s) 250, 255, 382, 446, 557
NlaIV GGNNCC 5 cut(s) 441, 493, 528, 565, 658
PaeR7I CTCGAG 1 cut(s) 242
PfeI GAWTC 1 cut(s) 227
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 5 cut(s) 145, 291, 294, 316, 541
PleI GAGTC 1 cut(s) 200
PluTI GGCGCC 1 cut(s) 530
PmeI GTTTAAAC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 200
PspN4I GGNNCC 5 cut(s) 441, 493, 528, 565, 658
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 5 cut(s) 330, 491, 492, 564, 612
PspXI VCTCGAGB 1 cut(s) 242
PsuI RGATCY 2 cut(s) 255, 557
RsaI GTAC 4 cut(s) 267, 388, 455, 658
RsaNI GTAC 4 cut(s) 266, 387, 454, 657
SaqAI TTAA 2 cut(s) 198, 627
SatI GCNGC 5 cut(s) 144, 290, 293, 315, 540
Sau3AI GATC 5 cut(s) 250, 255, 382, 446, 557
Sau96I GGNCC 5 cut(s) 330, 491, 492, 564, 612
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 272, 410
SduI GDGCHC 2 cut(s) 444, 495
SetI ASST 7 cut(s) 37, 135, 148, 285, 522, 658, 666
SfoI GGCGCC 1 cut(s) 528
Sfr274I CTCGAG 1 cut(s) 242
SinI GGWCC 1 cut(s) 564
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 128, 242
SmoI CTYRAG 2 cut(s) 128, 242
Sse9I AATT 1 cut(s) 499
SsiI CCGC 5 cut(s) 60, 75, 289, 292, 315
SspDI GGCGCC 1 cut(s) 526
StyD4I CCNGG 2 cut(s) 270, 408
TaqI TCGA 3 cut(s) 135, 243, 554
TaqII GACCGA 1 cut(s) 609
TasI AATT 1 cut(s) 499
TauI GCSGC 3 cut(s) 292, 295, 317
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 2 cut(s) 198, 627
Tru9I TTAA 2 cut(s) 198, 627
TseI GCWGC 2 cut(s) 143, 539
TspDTI ATGAA 2 cut(s) 32, 524
TspGWI ACGGA 4 cut(s) 7, 96, 103, 556
VpaK11BI GGWCC 1 cut(s) 564
XhoI CTCGAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.