pycom06g00100

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012234
Physical Location & Seq
Reverse (-)
133805 .. 134521
717 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00100.1

Sequence Viewer

Length: 717 bp
ATGGGTGGATGTTTGTCCATAGTCGTCGTCACGAGGAGTAGTTCTAGTGGAAGAGATGATGATGATTCCTCGTCCACTGCCAAAGTAATCTACATGAACGGATTTATCCGCGACTATCCAGTTCCCCTTAAAGTCTCCAAAGTCCTTGAGGCCGACCGATCATCGTCTTCGTCTTCGTCTTCGTTTATATGCCATTCCGACCGTTTGTACTACGACAATTACATCCCGGCTTTAGATTCCGAAGATGAGCTGGAGGCCAATCAGATCTATTTCGTATTCCCCAGATCCAATCTTCAACGCCGCCTCAGTGCCACCGATATGGCGGCCTTGGCAGTCAGAGCCACCCTCGCCCTCCAGAAAATTAATATCAATGCTTCTCCTTCTTCCTCCTCCTCCTCCAAGAAGGAAAAGGGAAATAAGGGAGGTGGACGCCGTAATTACAGCAAGGCCCGCATTTCTCCGGTAATTGAGATGACTAATGATAAATATGAAAACTCGTTGGCCGAGGACGACGGAGGTTATTTGAGCATGAATGAATTAACGATTGGAGGTGGAGGGTCATCAGCGTATAAAGGCCAAAAGATGAGTAGTAGCGCCCGCGGCGGTGGGTCATCGTCGGCAAGGAAATTGCAGAGATACACATCGAGAAAGGCAAAGTTGGCCGCTCGTTCTTTTAGGCTCAGATTGTTAACCATTCACGAAGGCTCCGTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

239

Amino Acids

25.79

Weight (kDa)

9.59

Isoelectric Point (pI)

68.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 234 5.8e-29 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 665
AccII CGCG 2 cut(s) 111, 600
AciI CCGC 8 cut(s) 109, 301, 323, 451, 598, 600, 603, 663
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 2 cut(s) 501, 660
AcyI GRCGYC 1 cut(s) 430
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 1 cut(s) 296
AjuI GAANNNNNNNTTGG 2 cut(s) 528, 560
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 279
AoxI GGCC 7 cut(s) 150, 255, 324, 447, 501, 574, 660
ArsI GACNNNNNNTTYG 1 cut(s) 693
AseI ATTAAT 1 cut(s) 363
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 227
BauI CACGAG 1 cut(s) 31
BbsI GAAGAC 3 cut(s) 159, 165, 171
BceAI ACGGC 1 cut(s) 417
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 597
BglII AGATCT 1 cut(s) 264
BisI GCNGC 4 cut(s) 301, 324, 601, 663
BlsI GCNGC 4 cut(s) 302, 325, 602, 664
Bme1390I CCNGG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 1 cut(s) 706
BmrFI CCNGG 1 cut(s) 227
BpiI GAAGAC 3 cut(s) 159, 165, 171
BplI GAGNNNNNCTC 2 cut(s) 330, 362
BpmI CTGGAG 2 cut(s) 272, 338
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 640
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 3 cut(s) 327, 504, 598
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 1 cut(s) 689
Bse1I ACTGG 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 640
BseDI CCNNGG 3 cut(s) 327, 504, 598
BseGI GGATG 2 cut(s) 14, 222
BseJI GATNNNNATC 1 cut(s) 640
BseMII CTCAG 2 cut(s) 319, 694
BseNI ACTGG 1 cut(s) 119
BseRI GAGGAG 4 cut(s) 49, 379, 382, 385
Bsh1236I CGCG 2 cut(s) 111, 600
Bsh1285I CGRYCG 2 cut(s) 157, 202
BshFI GGCC 7 cut(s) 152, 257, 326, 449, 503, 576, 662
BsiEI CGRYCG 2 cut(s) 157, 202
BsiSI CCGG 2 cut(s) 227, 461
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 7 cut(s) 152, 257, 326, 449, 503, 576, 662
Bsp143I GATC 3 cut(s) 158, 264, 284
BspACI CCGC 8 cut(s) 109, 301, 323, 451, 598, 600, 603, 663
BspANI GGCC 7 cut(s) 152, 257, 326, 449, 503, 576, 662
BspCNI CTCAG 2 cut(s) 318, 693
BspFNI CGCG 2 cut(s) 111, 600
BspLI GGNNCC 1 cut(s) 706
BspPI GGATC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 665
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 327, 504, 598
BssMI GATC 3 cut(s) 158, 264, 284
BssNI GRCGYC 1 cut(s) 430
BssSI CACGAG 1 cut(s) 31
BssT1I CCWWGG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 46
BstACI GRCGYC 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 451, 598
BstDEI CTNAG 2 cut(s) 305, 680
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 2 cut(s) 14, 222
BstFNI CGCG 2 cut(s) 111, 600
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 3 cut(s) 161, 267, 287
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 3 cut(s) 158, 264, 284
BstMCI CGRYCG 2 cut(s) 157, 202
BstMWI GCNNNNNNNGC 6 cut(s) 329, 338, 347, 450, 600, 659
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 2 cut(s) 111, 600
BstV2I GAAGAC 3 cut(s) 159, 165, 171
BstX2I RGATCY 2 cut(s) 264, 284
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 2 cut(s) 264, 284
BsuRI GGCC 7 cut(s) 152, 257, 326, 449, 503, 576, 662
BtgI CCRYGG 1 cut(s) 598
BtsCI GGATG 2 cut(s) 14, 222
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 2 cut(s) 75, 313
Cac8I GCNNGC 2 cut(s) 451, 598
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 1 cut(s) 448
Cfr42I CCGCGG 1 cut(s) 601
CseI GACGC 1 cut(s) 438
Csp6I GTAC 1 cut(s) 208
CviAII CATG 2 cut(s) 94, 529
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 305, 680
DpnI GATC 3 cut(s) 160, 266, 286
DpnII GATC 3 cut(s) 158, 264, 284
EaeI YGGCCR 2 cut(s) 501, 660
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 2 cut(s) 97, 532
FaiI YATR 8 cut(s) 20, 95, 188, 190, 320, 489, 530, 570
FatI CATG 2 cut(s) 93, 528
FauI CCCGC 2 cut(s) 458, 605
Fnu4HI GCNGC 4 cut(s) 301, 324, 601, 663
FokI GGATG 2 cut(s) 21, 209
Fsp4HI GCNGC 4 cut(s) 301, 324, 601, 663
FspBI CTAG 1 cut(s) 45
GlaI GCGC 1 cut(s) 595
GluI GCNGC 4 cut(s) 301, 324, 601, 663
GsuI CTGGAG 2 cut(s) 272, 338
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 7 cut(s) 152, 257, 326, 449, 503, 576, 662
HapII CCGG 2 cut(s) 227, 461
HgaI GACGC 1 cut(s) 438
HhaI GCGC 1 cut(s) 596
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 2 cut(s) 97, 532
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HinfI GANTC 2 cut(s) 65, 236
HpaI GTTAAC 1 cut(s) 690
HpaII CCGG 2 cut(s) 227, 461
Hpy166II GTNNAC 3 cut(s) 75, 428, 690
Hpy188I TCNGA 6 cut(s) 199, 241, 264, 338, 683, 716
Hpy188III TCNNGA 4 cut(s) 31, 355, 645, 698
Hpy8I GTNNAC 3 cut(s) 75, 428, 690
Hpy99I CGWCG 3 cut(s) 29, 515, 619
HpyAV CCTTC 3 cut(s) 390, 397, 695
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4V TGCA 1 cut(s) 631
HpyF10VI GCNNNNNNNGC 6 cut(s) 329, 338, 347, 450, 600, 659
HpyF3I CTNAG 2 cut(s) 305, 680
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 2 cut(s) 97, 532
HspAI GCGC 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 690
KspI CCGCGG 1 cut(s) 601
Kzo9I GATC 3 cut(s) 158, 264, 284
LmnI GCTCC 1 cut(s) 710
LpnPI CCDG 6 cut(s) 132, 236, 240, 295, 368, 474
MaeI CTAG 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 160, 266, 286
MbiI CCGCTC 1 cut(s) 665
MboI GATC 3 cut(s) 158, 264, 284
MboII GAAGA 7 cut(s) 63, 159, 165, 171, 254, 284, 375
MflI RGATCY 2 cut(s) 264, 284
MluCI AATT 6 cut(s) 217, 360, 436, 465, 536, 626
MmeI TCCRAC 1 cut(s) 222
MseI TTAA 4 cut(s) 129, 363, 539, 689
MslI CAYNNNNRTG 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 600
MspI CCGG 2 cut(s) 227, 461
MspR9I CCNGG 1 cut(s) 227
MvnI CGCG 2 cut(s) 111, 600
MwoI GCNNNNNNNGC 6 cut(s) 329, 338, 347, 450, 600, 659
NciI CCSGG 1 cut(s) 227
NdeII GATC 3 cut(s) 158, 264, 284
NlaIII CATG 2 cut(s) 97, 532
NlaIV GGNNCC 1 cut(s) 706
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PfeI GAWTC 2 cut(s) 65, 236
PkrI GCNGC 4 cut(s) 302, 325, 602, 664
PshBI ATTAAT 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 706
PspPI GGNCC 1 cut(s) 448
PsuI RGATCY 2 cut(s) 264, 284
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 317
SacII CCGCGG 1 cut(s) 601
SaqAI TTAA 4 cut(s) 129, 363, 539, 689
SatI GCNGC 4 cut(s) 301, 324, 601, 663
Sau3AI GATC 3 cut(s) 158, 264, 284
Sau96I GGNCC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 227
SetI ASST 4 cut(s) 252, 427, 520, 553
Sfr303I CCGCGG 1 cut(s) 601
SgrBI CCGCGG 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 6 cut(s) 217, 360, 436, 465, 536, 626
SsiI CCGC 8 cut(s) 109, 301, 323, 451, 598, 600, 603, 663
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 203
TaqI TCGA 1 cut(s) 644
TaqII GACCGA 1 cut(s) 171
TasI AATT 6 cut(s) 217, 360, 436, 465, 536, 626
TatI WGTACW 1 cut(s) 207
TauI GCSGC 4 cut(s) 303, 326, 603, 665
TfiI GAWTC 2 cut(s) 65, 236
Tru1I TTAA 4 cut(s) 129, 363, 539, 689
Tru9I TTAA 4 cut(s) 129, 363, 539, 689
TscAI CASTG 2 cut(s) 82, 313
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 4 cut(s) 110, 504, 545, 549
TspGWI ACGGA 3 cut(s) 114, 528, 697
TspRI CASTG 2 cut(s) 82, 313
VspI ATTAAT 1 cut(s) 363
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.