RLG00000002465

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012234
Physical Location & Seq
Reverse (-)
34551361 .. 34556660
5300 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002465

Sequence Viewer

Length: 966 bp
ATGGGTGGATGCTTCTCCGTGAGCGCTTTTGAACCTTCTCTTCGTTCTTCGTCTCCCACCGCCAAAGTTGTCTCCGCGAACGGAAGTCTCATGGAATACCCTGTTCCCGTTTCCGTCTCACAAGTCCTCGAGGCCGGGGAGGCAGCTTCTCCCTCTTCCTCCACGGCGTCGTTTTTCGTCTGCAACTCCGACCGGTTTAACTACGACTCTTATATTCCGGCTTTGGATTCCGACCACCAACTCGAGGCTGATCAGATCTACTTCGTACTCCCGGTTGCTAAGCTCCAGCGGCGGCTGACTGCCTCGGATATGGCCGCTTTCGCTGTCAGGGCCAGCCTCGCCCTCCAAAATGCGTCCACGGCTTCTTCCTCGGGAAAAAAGGATCGTACTCGTCGCCGGAACAAGAATCGGGTTTCTCCCATTCTCGTTGTCAAGTCGGAGCCCAGATCGGAGTACGATTTTGTGGATTACGAGGCTGATTTTACGATTGGGCCCTCCAGTTCTCTTATGAAGAAGAAGGTGATGGCGCCGGAGAAGGCTGCTTTGGGGGTTTCGAGATCTGGGTCCGTCCGGAAGTTGCAGAGATACTCATCTAAAAGAGTATTGAAGAGGGCCGTTCGGTCGTTTAAACTCAGATTGACCCCAATACAAGAAGGTACCGAGCAGTCGGTGCCTGTTTCTCTGCCAACTGCTATCCCAATACCTATCTCTGAGGCTCCAACTGCTGTCCTGGTACTCATCTCTGTAACGAATGATGTCCCGTTGTCAATTTCAAGGATGAAACAACCACATTTGTTTAGGCTGTTCCAGAGGGAAGCGCAGCTGTTTCCACTTTTTGCTGAAAGAGGAATTAATACTTCATCATCTGTTAGTGGTGATCAAGGGAATGGTGATGTTAGTGGTGATCAAGGGAATGGTGAGGTTGAATATGAGAATGACCTTGACCTGGAACTCACTGTAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

322

Amino Acids

34.79

Weight (kDa)

8.77

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 218 2.2e-29 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 128
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 3 cut(s) 526, 656, 670
AccII CGCG 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 570
AciI CCGC 5 cut(s) 60, 75, 289, 292, 315
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 312
AcyI GRCGYC 2 cut(s) 167, 527
AfaI GTAC 5 cut(s) 267, 388, 455, 658, 735
AfeI AGCGCT 1 cut(s) 25
AfiI CCNNNNNNNGG 2 cut(s) 244, 946
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 4 cut(s) 32, 607, 774, 926
AjnI CCWGG 2 cut(s) 729, 945
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AluBI AGCT 3 cut(s) 146, 283, 823
AluI AGCT 3 cut(s) 146, 283, 823
Alw26I GTCTC 4 cut(s) 57, 76, 92, 121
AlwI GGATC 1 cut(s) 390
Ama87I CYCGRG 3 cut(s) 128, 242, 370
Aor13HI TCCGGA 1 cut(s) 570
Aor51HI AGCGCT 1 cut(s) 25
AoxI GGCC 5 cut(s) 132, 312, 330, 491, 612
ApaI GGGCCC 1 cut(s) 495
ApeKI GCWGC 3 cut(s) 143, 539, 820
AseI ATTAAT 1 cut(s) 852
AsiGI ACCGGT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 3 cut(s) 26, 529, 820
AspS9I GGNCC 5 cut(s) 330, 491, 492, 564, 612
AsuC2I CCSGG 2 cut(s) 136, 272
AsuHPI GGTGA 5 cut(s) 532, 887, 902, 914, 929
AvaI CYCGRG 3 cut(s) 128, 242, 370
AvaII GGWCC 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 495
BanI GGYRCC 3 cut(s) 526, 656, 670
BanII GRGCYC 2 cut(s) 444, 495
BbvI GCAGC 3 cut(s) 155, 526, 832
BccI CCATC 1 cut(s) 517
BceAI ACGGC 3 cut(s) 180, 375, 599
BciT130I CCWGG 2 cut(s) 731, 947
BclI TGATCA 3 cut(s) 250, 877, 904
BcnI CCSGG 2 cut(s) 136, 272
BcoDI GTCTC 4 cut(s) 57, 76, 92, 121
BfmI CTRYAG 1 cut(s) 957
BfoI RGCGCY 2 cut(s) 27, 530
BglI GCCNNNNNGGC 1 cut(s) 140
BglII AGATCT 2 cut(s) 255, 557
BisI GCNGC 6 cut(s) 144, 290, 293, 315, 540, 821
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 6 cut(s) 145, 291, 294, 316, 541, 822
Bme1390I CCNGG 4 cut(s) 136, 272, 731, 947
Bme18I GGWCC 1 cut(s) 564
BmeT110I CYCGRG 3 cut(s) 128, 242, 370
BmgT120I GGNCC 5 cut(s) 330, 491, 492, 564, 612
BmiI GGNNCC 7 cut(s) 441, 493, 528, 565, 658, 672, 717
BmrFI CCNGG 4 cut(s) 136, 272, 731, 947
BpmI CTGGAG 2 cut(s) 269, 481
Bpu1102I GCTNAGC 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 136, 272
BsaBI GATNNNNATC 1 cut(s) 589
BsaHI GRCGYC 2 cut(s) 167, 527
BsaJI CCNNGG 5 cut(s) 135, 162, 303, 357, 369
BsaWI WCCGGW 2 cut(s) 192, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 946
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 589
BseAI TCCGGA 1 cut(s) 570
BseBI CCWGG 2 cut(s) 731, 947
BseDI CCNNGG 5 cut(s) 135, 162, 303, 357, 369
BseGI GGATG 2 cut(s) 14, 783
BseJI GATNNNNATC 1 cut(s) 589
BseLI CCNNNNNNNGG 2 cut(s) 244, 946
BseMII CTCAG 2 cut(s) 646, 702
BseNI ACTGG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 3 cut(s) 155, 526, 832
Bsh1236I CGCG 1 cut(s) 77
Bsh1285I CGRYCG 2 cut(s) 193, 623
BshFI GGCC 5 cut(s) 134, 314, 332, 493, 614
BshNI GGYRCC 3 cut(s) 526, 656, 670
BshTI ACCGGT 1 cut(s) 192
BsiEI CGRYCG 2 cut(s) 193, 623
BsiHKCI CYCGRG 3 cut(s) 128, 242, 370
BsiSI CCGG 7 cut(s) 135, 193, 218, 272, 397, 530, 571
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 2 cut(s) 244, 946
BsmAI GTCTC 4 cut(s) 57, 76, 92, 121
BsmBI CGTCTC 2 cut(s) 57, 121
BsmFI GGGAC 1 cut(s) 743
BsnI GGCC 5 cut(s) 134, 314, 332, 493, 614
BsoBI CYCGRG 3 cut(s) 128, 242, 370
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 444, 495
Bsp13I TCCGGA 1 cut(s) 570
Bsp143I GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 5 cut(s) 60, 75, 289, 292, 315
BspANI GGCC 5 cut(s) 134, 314, 332, 493, 614
BspCNI CTCAG 2 cut(s) 645, 703
BspEI TCCGGA 1 cut(s) 570
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 7 cut(s) 441, 493, 528, 565, 658, 672, 717
BspPI GGATC 1 cut(s) 390
BspT107I GGYRCC 3 cut(s) 526, 656, 670
BsrFI RCCGGY 1 cut(s) 192
BsrI ACTGG 1 cut(s) 498
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 5 cut(s) 135, 162, 303, 357, 369
BssMI GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
BssNI GRCGYC 2 cut(s) 167, 527
Bst2UI CCWGG 2 cut(s) 731, 947
Bst4CI ACNGT 1 cut(s) 958
Bst6I CTCTTC 3 cut(s) 45, 160, 602
BstACI GRCGYC 2 cut(s) 167, 527
BstAPI GCANNNNNTGC 1 cut(s) 670
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 3 cut(s) 279, 632, 711
BstDSI CCRYGG 2 cut(s) 162, 357
BstF5I GGATG 2 cut(s) 14, 783
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 2 cut(s) 27, 530
BstHHI GCGC 3 cut(s) 26, 529, 820
BstKTI GATC 7 cut(s) 253, 258, 385, 449, 560, 880, 907
BstMAI GTCTC 4 cut(s) 57, 76, 92, 121
BstMBI GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
BstMCI CGRYCG 2 cut(s) 193, 623
BstMWI GCNNNNNNNGC 8 cut(s) 140, 289, 320, 329, 338, 359, 670, 722
BstNI CCWGG 2 cut(s) 731, 947
BstSCI CCNGG 4 cut(s) 134, 270, 729, 945
BstSFI CTRYAG 1 cut(s) 957
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 3 cut(s) 155, 526, 832
BstX2I RGATCY 2 cut(s) 255, 557
BstYI RGATCY 2 cut(s) 255, 557
BsuRI GGCC 5 cut(s) 134, 314, 332, 493, 614
BtgI CCRYGG 2 cut(s) 162, 357
BtsCI GGATG 2 cut(s) 14, 783
BtsIMutI CAGTG 1 cut(s) 954
Cac8I GCNNGC 1 cut(s) 334
CfoI GCGC 3 cut(s) 26, 529, 820
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 5 cut(s) 330, 491, 492, 564, 612
CseI GACGC 2 cut(s) 156, 342
Csp6I GTAC 5 cut(s) 266, 387, 454, 657, 734
CspAI ACCGGT 1 cut(s) 192
CviAII CATG 1 cut(s) 91
CviQI GTAC 5 cut(s) 266, 387, 454, 657, 734
DdeI CTNAG 3 cut(s) 279, 632, 711
DinI GGCGCC 1 cut(s) 528
DpnI GATC 7 cut(s) 252, 257, 384, 448, 559, 879, 906
DpnII GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
DraI TTTAAA 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 3 cut(s) 45, 160, 602
EarI CTCTTC 3 cut(s) 45, 160, 602
Eco24I GRGCYC 2 cut(s) 444, 495
Eco47I GGWCC 1 cut(s) 564
Eco47III AGCGCT 1 cut(s) 25
Eco88I CYCGRG 3 cut(s) 128, 242, 370
EcoO109I RGGNCCY 1 cut(s) 492
EcoRII CCWGG 2 cut(s) 729, 945
EcoT38I GRGCYC 2 cut(s) 444, 495
EgeI GGCGCC 1 cut(s) 528
EheI GGCGCC 1 cut(s) 528
Esp3I CGTCTC 2 cut(s) 57, 121
FaeI CATG 1 cut(s) 94
FaiI YATR 5 cut(s) 92, 213, 311, 509, 930
FaqI GGGAC 1 cut(s) 743
FatI CATG 1 cut(s) 90
FbaI TGATCA 3 cut(s) 250, 877, 904
Fnu4HI GCNGC 6 cut(s) 144, 290, 293, 315, 540, 821
FokI GGATG 2 cut(s) 21, 790
FriOI GRGCYC 2 cut(s) 444, 495
Fsp4HI GCNGC 6 cut(s) 144, 290, 293, 315, 540, 821
GlaI GCGC 3 cut(s) 25, 528, 819
GluI GCNGC 6 cut(s) 144, 290, 293, 315, 540, 821
GsuI CTGGAG 2 cut(s) 269, 481
HaeII RGCGCY 2 cut(s) 27, 530
HaeIII GGCC 5 cut(s) 134, 314, 332, 493, 614
HapII CCGG 7 cut(s) 135, 193, 218, 272, 397, 530, 571
HgaI GACGC 2 cut(s) 156, 342
HhaI GCGC 3 cut(s) 26, 529, 820
Hin1I GRCGYC 2 cut(s) 167, 527
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 3 cut(s) 24, 527, 818
HinP1I GCGC 3 cut(s) 24, 527, 818
HinfI GANTC 3 cut(s) 206, 227, 406
HpaII CCGG 7 cut(s) 135, 193, 218, 272, 397, 530, 571
HphI GGTGA 5 cut(s) 532, 887, 902, 914, 929
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 8 cut(s) 190, 232, 255, 307, 439, 451, 635, 712
Hpy188III TCNNGA 4 cut(s) 372, 555, 571, 808
Hpy8I GTNNAC 1 cut(s) 357
Hpy99I CGWCG 2 cut(s) 172, 396
HpyAV CCTTC 4 cut(s) 45, 511, 529, 647
HpyCH4III ACNGT 1 cut(s) 958
HpyCH4V TGCA 2 cut(s) 183, 580
HpyF10VI GCNNNNNNNGC 8 cut(s) 140, 289, 320, 329, 338, 359, 670, 722
HpyF3I CTNAG 3 cut(s) 279, 632, 711
Hsp92I GRCGYC 2 cut(s) 167, 527
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 3 cut(s) 24, 527, 818
KasI GGCGCC 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 570
KpnI GGTACC 1 cut(s) 660
Ksp22I TGATCA 3 cut(s) 250, 877, 904
Kzo9I GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
LmnI GCTCC 3 cut(s) 288, 439, 721
Lsp1109I GCAGC 3 cut(s) 155, 526, 832
MaeIII GTNAC 1 cut(s) 745
MalI GATC 7 cut(s) 252, 257, 384, 448, 559, 879, 906
MboI GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
MboII GAAGA 7 cut(s) 32, 39, 147, 357, 523, 526, 619
MflI RGATCY 2 cut(s) 255, 557
MhlI GDGCHC 2 cut(s) 444, 495
MluCI AATT 2 cut(s) 768, 849
Mly113I GGCGCC 1 cut(s) 527
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 4 cut(s) 213, 255, 417, 743
MroI TCCGGA 1 cut(s) 570
MseI TTAA 3 cut(s) 198, 627, 852
MspA1I CMGCKG 2 cut(s) 289, 823
MspI CCGG 7 cut(s) 135, 193, 218, 272, 397, 530, 571
MspR9I CCNGG 4 cut(s) 136, 272, 731, 947
MssI GTTTAAAC 1 cut(s) 628
MvaI CCWGG 2 cut(s) 731, 947
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 8 cut(s) 140, 289, 320, 329, 338, 359, 670, 722
NarI GGCGCC 1 cut(s) 527
NciI CCSGG 2 cut(s) 136, 272
NdeII GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 7 cut(s) 441, 493, 528, 565, 658, 672, 717
PaeR7I CTCGAG 2 cut(s) 128, 242
PfeI GAWTC 2 cut(s) 227, 406
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 6 cut(s) 145, 291, 294, 316, 541, 822
PleI GAGTC 1 cut(s) 200
PluTI GGCGCC 1 cut(s) 530
PmeI GTTTAAAC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 200
PshBI ATTAAT 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 729, 945
PspGI CCWGG 2 cut(s) 729, 945
PspN4I GGNNCC 7 cut(s) 441, 493, 528, 565, 658, 672, 717
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 5 cut(s) 330, 491, 492, 564, 612
PspXI VCTCGAGB 2 cut(s) 128, 242
PsuI RGATCY 2 cut(s) 255, 557
PvuII CAGCTG 1 cut(s) 823
RsaI GTAC 5 cut(s) 267, 388, 455, 658, 735
RsaNI GTAC 5 cut(s) 266, 387, 454, 657, 734
SaqAI TTAA 3 cut(s) 198, 627, 852
SatI GCNGC 6 cut(s) 144, 290, 293, 315, 540, 821
Sau3AI GATC 7 cut(s) 250, 255, 382, 446, 557, 877, 904
Sau96I GGNCC 5 cut(s) 330, 491, 492, 564, 612
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 4 cut(s) 136, 272, 731, 947
SduI GDGCHC 2 cut(s) 444, 495
SfcI CTRYAG 1 cut(s) 957
SfoI GGCGCC 1 cut(s) 528
Sfr274I CTCGAG 2 cut(s) 128, 242
SinI GGWCC 1 cut(s) 564
SlaI CTCGAG 2 cut(s) 128, 242
SmlI CTYRAG 2 cut(s) 128, 242
SmoI CTYRAG 2 cut(s) 128, 242
Sse9I AATT 2 cut(s) 768, 849
SsiI CCGC 5 cut(s) 60, 75, 289, 292, 315
SspDI GGCGCC 1 cut(s) 526
StyD4I CCNGG 4 cut(s) 134, 270, 729, 945
TaaI ACNGT 1 cut(s) 958
TaqI TCGA 3 cut(s) 129, 243, 554
TaqII GACCGA 1 cut(s) 609
TasI AATT 2 cut(s) 768, 849
TauI GCSGC 3 cut(s) 292, 295, 317
TfiI GAWTC 2 cut(s) 227, 406
Tru1I TTAA 3 cut(s) 198, 627, 852
Tru9I TTAA 3 cut(s) 198, 627, 852
TscAI CASTG 1 cut(s) 961
TseI GCWGC 3 cut(s) 143, 539, 820
TspDTI ATGAA 3 cut(s) 524, 794, 849
TspGWI ACGGA 4 cut(s) 7, 96, 103, 556
TspRI CASTG 1 cut(s) 961
VpaK11BI GGWCC 1 cut(s) 564
VspI ATTAAT 1 cut(s) 852
XhoI CTCGAG 2 cut(s) 128, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.