Rmu_co8357791.1_g000001

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8357791.1
OG0012234
Physical Location & Seq
Reverse (-)
473 .. 1068
596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8357791.1_g000001.1.cds

Sequence Viewer

Length: 596 bp
cgaacggaagtctcatagaataccctgttcccgtttccgtctcccaagtcctcaaggccgaggaggcagcttctccctcttcctccacggcgtcgtttttcgtctgcaactccgaccggtttaactacgactcttatattcctgctttggtttcggaccaccaactcgacgctgatcagatctacttcgtactcccggttgctaagctccagcggcggctgactgcctcggatatggccgctttggctgttagggccagcctcgccctccaaaatgcgtccacggcttcttcctcgggaaaaaaggatcgcactcgtcgccggaacaagaaccgggtttctcccattctcgttgtcaagtcggagcccagatcggagtacaattttgtggattacgaggctgattttacgattgggccctccaattgtcttatgaagaagaaggtgatggcgccggagaaggctgctttgggggtttcgagatctgggtccgtccggaagttgcagagatactcatctaaaagagtattgaagagggccgttcggtcgtttaaactcagattgaccccaatacaagaaggtaccgaggtattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

197

Amino Acids

21.81

Weight (kDa)

9.96

Isoelectric Point (pI)

61.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21010 AT1G76600
fragaria_vesca FvH4_5g26600
malus_domestica MD07G1142400.v1.1
prunus_persica Prupe.5G001600_v2.0.a1
pyrus_communis pycom06g00100 pycom07g14120
rosa_chinensis RchiOBHm_Chr7g0217541
rosa_laevigata RLG00000002465
rosa_multiflora Rmu_co8357791.1_g000001 Rmu_sc0004284.1_g000003 Rmu_sc0019291.1_g000003
rosa_roxburghii Rroxscaffold_3G00241920
rosa_rugosa Rorug07G0169700
rosa_samantha Rh7AG309400 Rh7BG300200 Rh7CG328600 Rh7DG308900
rosa_wichuraiana Rw7G026240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 580
AccB1I GGYRCC 2 cut(s) 450, 580
AccIII TCCGGA 1 cut(s) 494
AciI CCGC 3 cut(s) 213, 216, 239
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 1 cut(s) 236
AcyI GRCGYC 2 cut(s) 91, 451
AfaI GTAC 3 cut(s) 191, 379, 582
AgeI ACCGGT 1 cut(s) 116
AgsI TTSAA 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 451, 483
AluBI AGCT 2 cut(s) 70, 207
AluI AGCT 2 cut(s) 70, 207
Alw26I GTCTC 2 cut(s) 16, 45
AlwI GGATC 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 294
Aor13HI TCCGGA 1 cut(s) 494
AoxI GGCC 5 cut(s) 56, 236, 254, 415, 536
ApaI GGGCCC 1 cut(s) 419
ApeKI GCWGC 2 cut(s) 67, 463
AsiGI ACCGGT 1 cut(s) 116
Asp718I GGTACC 1 cut(s) 580
AspLEI GCGC 1 cut(s) 453
AspS9I GGNCC 6 cut(s) 156, 254, 415, 416, 488, 536
AsuC2I CCSGG 2 cut(s) 196, 334
AsuHPI GGTGA 1 cut(s) 456
AvaI CYCGRG 1 cut(s) 294
AvaII GGWCC 2 cut(s) 156, 488
BaeGI GKGCMC 1 cut(s) 419
BaeI ACNNNNGTAYC 1 cut(s) 572
BanI GGYRCC 2 cut(s) 450, 580
BanII GRGCYC 2 cut(s) 368, 419
BbvI GCAGC 2 cut(s) 79, 450
BccI CCATC 1 cut(s) 441
BceAI ACGGC 3 cut(s) 104, 299, 523
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 2 cut(s) 196, 334
BcoDI GTCTC 2 cut(s) 16, 45
BfoI RGCGCY 1 cut(s) 454
BglI GCCNNNNNGGC 2 cut(s) 64, 244
BglII AGATCT 2 cut(s) 179, 481
BisI GCNGC 5 cut(s) 68, 214, 217, 239, 464
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 5 cut(s) 69, 215, 218, 240, 465
Bme1390I CCNGG 2 cut(s) 196, 334
Bme18I GGWCC 2 cut(s) 156, 488
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 6 cut(s) 156, 254, 415, 416, 488, 536
BmiI GGNNCC 5 cut(s) 365, 417, 452, 489, 582
BmrFI CCNGG 2 cut(s) 196, 334
BpmI CTGGAG 1 cut(s) 193
Bpu1102I GCTNAGC 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 37
BpuMI CCSGG 2 cut(s) 196, 334
BsaBI GATNNNNATC 1 cut(s) 513
BsaHI GRCGYC 2 cut(s) 91, 451
BsaJI CCNNGG 6 cut(s) 59, 86, 227, 281, 293, 584
BsaWI WCCGGW 2 cut(s) 116, 494
Bse118I RCCGGY 1 cut(s) 116
Bse8I GATNNNNATC 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 494
BseDI CCNNGG 6 cut(s) 59, 86, 227, 281, 293, 584
BseJI GATNNNNATC 1 cut(s) 513
BseMII CTCAG 1 cut(s) 570
BseRI GAGGAG 1 cut(s) 76
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 2 cut(s) 79, 450
Bsh1285I CGRYCG 2 cut(s) 117, 547
BshFI GGCC 5 cut(s) 58, 238, 256, 417, 538
BshNI GGYRCC 2 cut(s) 450, 580
BshTI ACCGGT 1 cut(s) 116
BsiEI CGRYCG 2 cut(s) 117, 547
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 6 cut(s) 117, 196, 321, 333, 454, 495
BsmAI GTCTC 2 cut(s) 16, 45
BsmBI CGTCTC 1 cut(s) 45
BsnI GGCC 5 cut(s) 58, 238, 256, 417, 538
BsoBI CYCGRG 1 cut(s) 294
Bsp120I GGGCCC 1 cut(s) 415
Bsp1286I GDGCHC 2 cut(s) 368, 419
Bsp13I TCCGGA 1 cut(s) 494
Bsp143I GATC 5 cut(s) 174, 179, 306, 370, 481
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 3 cut(s) 213, 216, 239
BspANI GGCC 5 cut(s) 58, 238, 256, 417, 538
BspCNI CTCAG 1 cut(s) 569
BspEI TCCGGA 1 cut(s) 494
BspLI GGNNCC 5 cut(s) 365, 417, 452, 489, 582
BspPI GGATC 1 cut(s) 314
BspT107I GGYRCC 2 cut(s) 450, 580
BsrFI RCCGGY 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 6 cut(s) 59, 86, 227, 281, 293, 584
BssMI GATC 5 cut(s) 174, 179, 306, 370, 481
BssNI GRCGYC 2 cut(s) 91, 451
Bst6I CTCTTC 2 cut(s) 84, 526
BstACI GRCGYC 2 cut(s) 91, 451
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 2 cut(s) 203, 556
BstDSI CCRYGG 2 cut(s) 86, 281
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 5 cut(s) 177, 182, 309, 373, 484
BstMAI GTCTC 2 cut(s) 16, 45
BstMBI GATC 5 cut(s) 174, 179, 306, 370, 481
BstMCI CGRYCG 2 cut(s) 117, 547
BstMWI GCNNNNNNNGC 7 cut(s) 64, 213, 244, 253, 262, 283, 317
BstSCI CCNGG 2 cut(s) 194, 332
BstSLI GKGCMC 1 cut(s) 419
BstV1I GCAGC 2 cut(s) 79, 450
BstX2I RGATCY 2 cut(s) 179, 481
BstYI RGATCY 2 cut(s) 179, 481
BsuRI GGCC 5 cut(s) 58, 238, 256, 417, 538
BtgI CCRYGG 2 cut(s) 86, 281
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 1 cut(s) 453
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 6 cut(s) 156, 254, 415, 416, 488, 536
CseI GACGC 3 cut(s) 80, 178, 266
Csp6I GTAC 3 cut(s) 190, 378, 581
CspAI ACCGGT 1 cut(s) 116
CviQI GTAC 3 cut(s) 190, 378, 581
DdeI CTNAG 2 cut(s) 203, 556
DinI GGCGCC 1 cut(s) 452
DpnI GATC 5 cut(s) 176, 181, 308, 372, 483
DpnII GATC 5 cut(s) 174, 179, 306, 370, 481
DraI TTTAAA 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 236
Eam1104I CTCTTC 2 cut(s) 84, 526
EarI CTCTTC 2 cut(s) 84, 526
Eco24I GRGCYC 2 cut(s) 368, 419
Eco47I GGWCC 2 cut(s) 156, 488
Eco88I CYCGRG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 416
EcoT38I GRGCYC 2 cut(s) 368, 419
EgeI GGCGCC 1 cut(s) 452
EheI GGCGCC 1 cut(s) 452
Esp3I CGTCTC 1 cut(s) 45
FaiI YATR 4 cut(s) 16, 137, 235, 433
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 68, 214, 217, 239, 464
FriOI GRGCYC 2 cut(s) 368, 419
Fsp4HI GCNGC 5 cut(s) 68, 214, 217, 239, 464
GlaI GCGC 1 cut(s) 452
GluI GCNGC 5 cut(s) 68, 214, 217, 239, 464
GsuI CTGGAG 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 5 cut(s) 58, 238, 256, 417, 538
HapII CCGG 6 cut(s) 117, 196, 321, 333, 454, 495
HgaI GACGC 3 cut(s) 80, 178, 266
HhaI GCGC 1 cut(s) 453
Hin1I GRCGYC 2 cut(s) 91, 451
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HinfI GANTC 1 cut(s) 130
HpaII CCGG 6 cut(s) 117, 196, 321, 333, 454, 495
HphI GGTGA 1 cut(s) 456
Hpy166II GTNNAC 1 cut(s) 281
Hpy188I TCNGA 7 cut(s) 114, 156, 179, 231, 363, 375, 559
Hpy188III TCNNGA 3 cut(s) 296, 479, 495
Hpy8I GTNNAC 1 cut(s) 281
Hpy99I CGWCG 3 cut(s) 96, 172, 320
HpyAV CCTTC 3 cut(s) 435, 453, 571
HpyCH4V TGCA 2 cut(s) 107, 504
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 213, 244, 253, 262, 283, 317
HpyF3I CTNAG 2 cut(s) 203, 556
Hsp92I GRCGYC 2 cut(s) 91, 451
HspAI GCGC 1 cut(s) 451
KasI GGCGCC 1 cut(s) 450
Kpn2I TCCGGA 1 cut(s) 494
KpnI GGTACC 1 cut(s) 584
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 5 cut(s) 174, 179, 306, 370, 481
LmnI GCTCC 2 cut(s) 212, 363
Lsp1109I GCAGC 2 cut(s) 79, 450
MalI GATC 5 cut(s) 176, 181, 308, 372, 483
MboI GATC 5 cut(s) 174, 179, 306, 370, 481
MboII GAAGA 5 cut(s) 71, 281, 447, 450, 543
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 2 cut(s) 179, 481
MhlI GDGCHC 2 cut(s) 368, 419
MluCI AATT 2 cut(s) 381, 423
Mly113I GGCGCC 1 cut(s) 451
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 2 cut(s) 137, 341
MroI TCCGGA 1 cut(s) 494
MseI TTAA 2 cut(s) 122, 551
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 6 cut(s) 117, 196, 321, 333, 454, 495
MspR9I CCNGG 2 cut(s) 196, 334
MssI GTTTAAAC 1 cut(s) 552
MunI CAATTG 1 cut(s) 423
MwoI GCNNNNNNNGC 7 cut(s) 64, 213, 244, 253, 262, 283, 317
NarI GGCGCC 1 cut(s) 451
NciI CCSGG 2 cut(s) 196, 334
NdeII GATC 5 cut(s) 174, 179, 306, 370, 481
NlaIV GGNNCC 5 cut(s) 365, 417, 452, 489, 582
NmeAIII GCCGAG 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 116
PkrI GCNGC 5 cut(s) 69, 215, 218, 240, 465
PleI GAGTC 1 cut(s) 124
PluTI GGCGCC 1 cut(s) 454
PmeI GTTTAAAC 1 cut(s) 552
PpsI GAGTC 1 cut(s) 124
PspN4I GGNNCC 5 cut(s) 365, 417, 452, 489, 582
PspOMI GGGCCC 1 cut(s) 415
PspPI GGNCC 6 cut(s) 156, 254, 415, 416, 488, 536
PsuI RGATCY 2 cut(s) 179, 481
RsaI GTAC 3 cut(s) 191, 379, 582
RsaNI GTAC 3 cut(s) 190, 378, 581
SaqAI TTAA 2 cut(s) 122, 551
SatI GCNGC 5 cut(s) 68, 214, 217, 239, 464
Sau3AI GATC 5 cut(s) 174, 179, 306, 370, 481
Sau96I GGNCC 6 cut(s) 156, 254, 415, 416, 488, 536
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 196, 334
SduI GDGCHC 2 cut(s) 368, 419
SetI ASST 5 cut(s) 72, 209, 446, 582, 590
SfoI GGCGCC 1 cut(s) 452
SinI GGWCC 2 cut(s) 156, 488
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 2 cut(s) 381, 423
SsiI CCGC 3 cut(s) 213, 216, 239
SspDI GGCGCC 1 cut(s) 450
StyD4I CCNGG 2 cut(s) 194, 332
TaqI TCGA 2 cut(s) 167, 478
TaqII GACCGA 1 cut(s) 533
TasI AATT 2 cut(s) 381, 423
TatI WGTACW 1 cut(s) 377
TauI GCSGC 3 cut(s) 216, 219, 241
Tru1I TTAA 2 cut(s) 122, 551
Tru9I TTAA 2 cut(s) 122, 551
TseI GCWGC 2 cut(s) 67, 463
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 3 cut(s) 20, 27, 480
VpaK11BI GGWCC 2 cut(s) 156, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.