AT1G21060

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005669
Physical Location & Seq
Forward (+)
7371657 .. 7374114
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21060.1

Sequence Viewer

Length: 1554 bp
ATGAATCTCTACAAACAGACAAGAAAGTGCGAGAAAATGATGGGTCTCTGTTCTCCTTCTCCTTCTTCTCACGATTGTTCTTCATCTTCTCCAAGGAACTCGGAGAAGAAGCAGAATGTGATTGTTGCATCACCTTCAAGATTCTCTACCTACACTAAACCGAGTTTATCAATTGAGAAGAGATTACTTACTTACCAGGATTCTAATTGCAACGTTACGCCAAAGTCTTCAGAAGATTTGAGGAAAGAAATTGCTTCACTTGAGTTTGAGATTCTGCGTACAGAACAATATCTGCTCTCTCTGTATAGGACAGCTTTCGATGAACAAGTTTCTTCTTTTTCTCCTCACACTGAAACAAGCTTAGTTTCTAATCAGTTTTTACCCAAATCCGAGCAATCAGATGTTACCAGTGTGTTCAGCTACCATTATCAAGCTTCTCCGGCCTCTGAATGTTCCTCTTTGTGTCCTCCTCGGAGTTTCCAAGCCAGCTTAAAAGCTTTGTCTGCAAGAGAGAAGAGTAGATATGTTTCTAGTAGTCACACTACTCTTGGAGATCTTCTGGGTTCTACTCTTATCGTAGATAACATTGCGAATCCGAGTAGGCTCTCTGAAGATATCTTAAGATGCATATGTTCTGTATATTGTACACTCTCCAGCAAAGCCAGAATCAATTCTTGTCTTCAGGCTTCTCCTTCTTCACCTTCATCTGTCTCTTCCAAAGCCACTTTTGATAGCTTGAACTCGCGCCATGAGGAACGCAAAGAAGCAAATGTGCCAGGCGTTGTTGTAATAGAATCTCTAGAACTTCACTTGGATGATGGTAGTTTCAATCACGCTGCTGTTATGCTCCAAAACTTCAGATCACTAGTTCAAAAACTTGAGAAGGTTGATCCGAGTAGAATGAAACGCGAAGAGAAGCTAGCGTTTTGGATAAACATTCACAATGCCCTCACAATGCATGCGTATCTAGCCTACGGAACTCACAACCGTGCCAGAAACACCTCGGTTTTAAAGGCAGCTTATGATGTTGGAGGCTATAGCGTAAACCCTTACATTATCCAAAGCTCAATCTTAGGCATTCGCCCACATTTTTCACAACCTTTGTTGCAGACATTGTTTTCGCCATCAAGAAAGTCGAAAACTTGTAATGTCAAACACATTTACGCCTTGGAATATCCCGAGGCATTGGCTCATTTTGCTCTTTCTTCAGGATTTTCTACTGATCCTCCGGTTCGGGTTTACACGGCAGATTGCGTATTCCGGGATCTAAGAAAATCCAAAGAAGAGTTTATAAGAAACAATGTTCGTATACACAACGAGACAAAGATATTGTTGCCCAAGATTGTTCATTACTATGCTAAAGATATGTCACTGGAACCATCTGCCCTCATGGAAACAACGGTGAAGTGTTTACCGGACTCCACGAAGAGAACAGCTCAGAAATTGTTGAAGAAGAAGAGCAGAAACATTGAATATTCACCGGAAAACTCAAGTTTCCGTTATGTAATCATCCAGGAACCAACAACAAGAAGACATGATTCTACTATAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1 UPF0047

Protein Analysis

517

Amino Acids

58.26

Weight (kDa)

9.13

Isoelectric Point (pI)

70.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 68 - 108 3.4e-06 Leucine-zipper of ternary complex factor MIP1
DUF547 PF04784 299 - 431 3.5e-32 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1292
AccI GTMKAC 1 cut(s) 1309
AccII CGCG 2 cut(s) 745, 909
AclI AACGTT 1 cut(s) 213
AclWI GGATC 3 cut(s) 884, 1217, 1272
AcuI CTGAAG 5 cut(s) 213, 630, 665, 841, 1191
AfaI GTAC 2 cut(s) 280, 646
AflII CTTAAG 1 cut(s) 619
AgsI TTSAA 6 cut(s) 138, 739, 829, 872, 1450, 1472
AhlI ACTAGT 1 cut(s) 865
AjnI CCWGG 3 cut(s) 195, 775, 1512
AleI CACNNNNGTG 1 cut(s) 987
Alw26I GTCTC 3 cut(s) 50, 715, 1313
AlwI GGATC 3 cut(s) 884, 1217, 1272
Ama87I CYCGRG 1 cut(s) 1178
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 2 cut(s) 836, 1016
ArsI GACNNNNNNTTYG 2 cut(s) 1102, 1134
Asp700I GAANNNNTTC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 747
AsuC2I CCSGG 1 cut(s) 1262
AsuHPI GGTGA 4 cut(s) 123, 690, 1414, 1470
AsuNHI GCTAGC 1 cut(s) 919
AvaI CYCGRG 1 cut(s) 1178
BbsI GAAGAC 3 cut(s) 219, 671, 1537
BbvI GCAGC 2 cut(s) 823, 1028
BccI CCATC 4 cut(s) 34, 812, 1132, 1387
BceAI ACGGC 1 cut(s) 1260
BciT130I CCWGG 3 cut(s) 197, 777, 1514
BcnI CCSGG 1 cut(s) 1262
BcoDI GTCTC 3 cut(s) 50, 715, 1313
BcuI ACTAGT 1 cut(s) 865
BfaI CTAG 5 cut(s) 531, 800, 866, 920, 968
BfmI CTRYAG 1 cut(s) 1036
BfrI CTTAAG 1 cut(s) 619
BglII AGATCT 1 cut(s) 553
BisI GCNGC 2 cut(s) 837, 1017
BlsI GCNGC 2 cut(s) 838, 1018
Bme1390I CCNGG 4 cut(s) 197, 777, 1262, 1514
BmeT110I CYCGRG 1 cut(s) 1178
BmiI GGNNCC 2 cut(s) 1377, 1518
BmrFI CCNGG 4 cut(s) 197, 777, 1262, 1514
BmsI GCATC 2 cut(s) 137, 614
BmtI GCTAGC 1 cut(s) 923
BpiI GAAGAC 3 cut(s) 219, 671, 1537
BplI GAGNNNNNCTC 2 cut(s) 1420, 1452
BpmI CTGGAG 1 cut(s) 637
BpuEI CTTGAG 3 cut(s) 281, 899, 1474
BpuMI CCSGG 1 cut(s) 1262
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 5 cut(s) 92, 470, 1002, 1167, 1179
BsaWI WCCGGW 3 cut(s) 1228, 1414, 1480
BsaXI ACNNNNNCTCC 2 cut(s) 1210, 1240
Bse1I ACTGG 2 cut(s) 408, 1377
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 3 cut(s) 197, 777, 1514
BseDI CCNNGG 5 cut(s) 92, 470, 1002, 1167, 1179
BseGI GGATG 2 cut(s) 820, 1509
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 1451
BseNI ACTGG 2 cut(s) 408, 1377
BseRI GAGGAG 2 cut(s) 333, 459
BseXI GCAGC 2 cut(s) 823, 1028
Bsh1236I CGCG 2 cut(s) 745, 909
BshFI GGCC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 1178
BsiSI CCGG 5 cut(s) 440, 1229, 1261, 1415, 1481
BsmAI GTCTC 3 cut(s) 50, 715, 1313
BsmI GAATGC 1 cut(s) 1077
BsnI GGCC 1 cut(s) 443
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 1178
Bsp1407I TGTACA 1 cut(s) 644
Bsp143I GATC 5 cut(s) 553, 860, 889, 1222, 1264
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 1 cut(s) 1450
BspFNI CGCG 2 cut(s) 745, 909
BspLI GGNNCC 2 cut(s) 1377, 1518
BspOI GCTAGC 1 cut(s) 923
BspPI GGATC 3 cut(s) 884, 1217, 1272
BspQI GCTCTTC 1 cut(s) 1451
BspTI CTTAAG 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 585
BsrGI TGTACA 1 cut(s) 644
BsrI ACTGG 2 cut(s) 408, 1377
BssECI CCNNGG 5 cut(s) 92, 470, 1002, 1167, 1179
BssMI GATC 5 cut(s) 553, 860, 889, 1222, 1264
BssNAI GTATAC 1 cut(s) 1310
BssT1I CCWWGG 2 cut(s) 92, 1167
Bst1107I GTATAC 1 cut(s) 1310
Bst2UI CCWGG 3 cut(s) 197, 777, 1514
Bst4CI ACNGT 2 cut(s) 989, 1402
Bst6I CTCTTC 7 cut(s) 173, 509, 718, 906, 1278, 1421, 1451
BstAFI CTTAAG 1 cut(s) 619
BstAUI TGTACA 1 cut(s) 644
BstC8I GCNNGC 3 cut(s) 487, 921, 960
BstDEI CTNAG 4 cut(s) 361, 1072, 1268, 1437
BstF5I GGATG 2 cut(s) 820, 1509
BstFNI CGCG 2 cut(s) 745, 909
BstHHI GCGC 1 cut(s) 747
BstKTI GATC 5 cut(s) 556, 863, 892, 1225, 1267
BstMAI GTCTC 3 cut(s) 50, 715, 1313
BstMBI GATC 5 cut(s) 553, 860, 889, 1222, 1264
BstMWI GCNNNNNNNGC 4 cut(s) 440, 503, 968, 1196
BstNI CCWGG 3 cut(s) 197, 777, 1514
BstNSI RCATGY 1 cut(s) 962
BstSCI CCNGG 4 cut(s) 195, 775, 1260, 1512
BstSFI CTRYAG 1 cut(s) 1036
BstUI CGCG 2 cut(s) 745, 909
BstV1I GCAGC 2 cut(s) 823, 1028
BstV2I GAAGAC 3 cut(s) 219, 671, 1537
BstX2I RGATCY 2 cut(s) 553, 1264
BstYI RGATCY 2 cut(s) 553, 1264
BstZ17I GTATAC 1 cut(s) 1310
BsuRI GGCC 1 cut(s) 443
BtsCI GGATG 2 cut(s) 820, 1509
BtsIMutI CAGTG 3 cut(s) 348, 415, 1370
Cac8I GCNNGC 3 cut(s) 487, 921, 960
CfoI GCGC 1 cut(s) 747
Csp6I GTAC 2 cut(s) 279, 645
CviAII CATG 4 cut(s) 749, 959, 1390, 1535
CviQI GTAC 2 cut(s) 279, 645
DdeI CTNAG 4 cut(s) 361, 1072, 1268, 1437
DpnI GATC 5 cut(s) 555, 862, 891, 1224, 1266
DpnII GATC 5 cut(s) 553, 860, 889, 1222, 1264
DraI TTTAAA 1 cut(s) 1011
Eam1104I CTCTTC 7 cut(s) 173, 509, 718, 906, 1278, 1421, 1451
EarI CTCTTC 7 cut(s) 173, 509, 718, 906, 1278, 1421, 1451
Eco130I CCWWGG 2 cut(s) 92, 1167
Eco31I GGTCTC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 616
Eco57I CTGAAG 5 cut(s) 213, 630, 665, 841, 1191
Eco88I CYCGRG 1 cut(s) 1178
EcoRII CCWGG 3 cut(s) 195, 775, 1512
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 92, 1167
EcoT22I ATGCAT 2 cut(s) 629, 960
ErhI CCWWGG 2 cut(s) 92, 1167
FaeI CATG 4 cut(s) 752, 962, 1393, 1538
FatI CATG 4 cut(s) 748, 958, 1389, 1534
FauNDI CATATG 1 cut(s) 629
FblI GTMKAC 1 cut(s) 1309
Fnu4HI GCNGC 2 cut(s) 837, 1017
FokI GGATG 2 cut(s) 827, 1496
Fsp4HI GCNGC 2 cut(s) 837, 1017
FspBI CTAG 5 cut(s) 531, 800, 866, 920, 968
GlaI GCGC 1 cut(s) 746
GluI GCNGC 2 cut(s) 837, 1017
GsuI CTGGAG 1 cut(s) 637
HaeIII GGCC 1 cut(s) 443
HapII CCGG 5 cut(s) 440, 1229, 1261, 1415, 1481
HhaI GCGC 1 cut(s) 747
Hin1II CATG 4 cut(s) 752, 962, 1393, 1538
Hin6I GCGC 1 cut(s) 745
HinP1I GCGC 1 cut(s) 745
HindIII AAGCTT 3 cut(s) 358, 432, 495
HinfI GANTC 9 cut(s) 4, 141, 200, 271, 592, 666, 794, 1418, 1538
HpaII CCGG 5 cut(s) 440, 1229, 1261, 1415, 1481
HphI GGTGA 4 cut(s) 123, 690, 1414, 1470
Hpy166II GTNNAC 5 cut(s) 647, 1045, 1240, 1310, 1412
Hpy188III TCNNGA 6 cut(s) 71, 138, 800, 1128, 1178, 1209
Hpy8I GTNNAC 5 cut(s) 647, 1045, 1240, 1310, 1412
HpyAV CCTTC 6 cut(s) 66, 72, 144, 702, 711, 877
HpyCH4III ACNGT 2 cut(s) 989, 1402
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 6 cut(s) 128, 210, 506, 627, 958, 1108
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 503, 968, 1196
HpyF3I CTNAG 4 cut(s) 361, 1072, 1268, 1437
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 4 cut(s) 752, 962, 1393, 1538
HspAI GCGC 1 cut(s) 745
Kzo9I GATC 5 cut(s) 553, 860, 889, 1222, 1264
LguI GCTCTTC 1 cut(s) 1451
LmnI GCTCC 1 cut(s) 852
Lsp1109I GCAGC 2 cut(s) 823, 1028
LweI GCATC 2 cut(s) 137, 614
MaeI CTAG 5 cut(s) 531, 800, 866, 920, 968
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 214, 403, 536, 1368
MalI GATC 5 cut(s) 555, 862, 891, 1224, 1266
MboI GATC 5 cut(s) 553, 860, 889, 1222, 1264
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 2 cut(s) 553, 1264
MluCI AATT 5 cut(s) 171, 205, 249, 670, 1442
MlyI GAGTC 1 cut(s) 1412
MmeI TCCRAC 1 cut(s) 1009
Mph1103I ATGCAT 2 cut(s) 629, 960
MroXI GAANNNNTTC 1 cut(s) 670
MseI TTAA 3 cut(s) 491, 620, 1010
MslI CAYNNNNRTG 3 cut(s) 813, 987, 1353
MspCI CTTAAG 1 cut(s) 619
MspI CCGG 5 cut(s) 440, 1229, 1261, 1415, 1481
MspR9I CCNGG 4 cut(s) 197, 777, 1262, 1514
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 1077
MvaI CCWGG 3 cut(s) 197, 777, 1514
MvnI CGCG 2 cut(s) 745, 909
MwoI GCNNNNNNNGC 4 cut(s) 440, 503, 968, 1196
NciI CCSGG 1 cut(s) 1262
NdeI CATATG 1 cut(s) 629
NdeII GATC 5 cut(s) 553, 860, 889, 1222, 1264
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 4 cut(s) 752, 962, 1393, 1538
NlaIV GGNNCC 2 cut(s) 1377, 1518
NmuCI GTSAC 2 cut(s) 536, 1368
NsiI ATGCAT 2 cut(s) 629, 960
NspI RCATGY 1 cut(s) 962
OliI CACNNNNGTG 1 cut(s) 987
PaeI GCATGC 1 cut(s) 962
PciSI GCTCTTC 1 cut(s) 1451
PctI GAATGC 1 cut(s) 1077
PdmI GAANNNNTTC 1 cut(s) 670
PfeI GAWTC 8 cut(s) 4, 141, 200, 271, 592, 666, 794, 1538
PfoI TCCNGGA 2 cut(s) 1260, 1512
PkrI GCNGC 2 cut(s) 838, 1018
PleI GAGTC 1 cut(s) 1412
PpsI GAGTC 1 cut(s) 1412
PsiI TTATAA 1 cut(s) 1292
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 3 cut(s) 195, 775, 1512
PspGI CCWGG 3 cut(s) 195, 775, 1512
PspN4I GGNNCC 2 cut(s) 1377, 1518
PsuI RGATCY 2 cut(s) 553, 1264
RsaI GTAC 2 cut(s) 280, 646
RsaNI GTAC 2 cut(s) 279, 645
RseI CAYNNNNRTG 3 cut(s) 813, 987, 1353
SapI GCTCTTC 1 cut(s) 1451
SaqAI TTAA 3 cut(s) 491, 620, 1010
SatI GCNGC 2 cut(s) 837, 1017
Sau3AI GATC 5 cut(s) 553, 860, 889, 1222, 1264
SchI GAGTC 1 cut(s) 1412
ScrFI CCNGG 4 cut(s) 197, 777, 1262, 1514
SfaNI GCATC 2 cut(s) 137, 614
SfcI CTRYAG 1 cut(s) 1036
SmiMI CAYNNNNRTG 3 cut(s) 813, 987, 1353
SmlI CTYRAG 4 cut(s) 260, 619, 878, 1489
SmoI CTYRAG 4 cut(s) 260, 619, 878, 1489
SpeI ACTAGT 1 cut(s) 865
SphI GCATGC 1 cut(s) 962
Sse9I AATT 5 cut(s) 171, 205, 249, 670, 1442
SspI AATATT 1 cut(s) 1475
SspMI CTAG 5 cut(s) 531, 800, 866, 920, 968
StyD4I CCNGG 4 cut(s) 195, 775, 1260, 1512
StyI CCWWGG 2 cut(s) 92, 1167
TaaI ACNGT 2 cut(s) 989, 1402
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 318, 1136
TasI AATT 5 cut(s) 171, 205, 249, 670, 1442
TatI WGTACW 1 cut(s) 644
TfiI GAWTC 8 cut(s) 4, 141, 200, 271, 592, 666, 794, 1538
Tru1I TTAA 3 cut(s) 491, 620, 1010
Tru9I TTAA 3 cut(s) 491, 620, 1010
TscAI CASTG 3 cut(s) 355, 415, 1377
TseFI GTSAC 2 cut(s) 536, 1368
TseI GCWGC 2 cut(s) 836, 1016
Tsp45I GTSAC 2 cut(s) 536, 1368
TspDTI ATGAA 6 cut(s) 17, 72, 336, 693, 917, 1337
TspGWI ACGGA 2 cut(s) 990, 1487
TspRI CASTG 3 cut(s) 355, 415, 1377
Vha464I CTTAAG 1 cut(s) 619
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 1 cut(s) 962
XmiI GTMKAC 1 cut(s) 1309
XmnI GAANNNNTTC 1 cut(s) 670
XspI CTAG 5 cut(s) 531, 800, 866, 920, 968
Zsp2I ATGCAT 2 cut(s) 629, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.