Rroxscaffold_3G00241880

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005669
Physical Location & Seq
Reverse (-)
34168268 .. 34173520
5253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00241880.1

Sequence Viewer

Length: 1281 bp
ATGAATTCCCGAAGAGCTTCTGATAATGGGCTTCTTCAAAATATACTTGGCAAGTCTCCTAGACTATTCTCCTACTTTAACCCTATTGCACAGTTGGACAGGATTTTACCTGACCAGGCTTCCCTATGTTGTGACTCGGAGAAGGCACTGTGTTGTAGTGTCATCAACTCTTCTGCTTCAGATGACACTCCAACACCAAAGGACCAGAAAACCGCTGATTCTCGTCATCGCAGTCGCAGCCTTGGGGATCATCTAGGTGCTTCTCGCATTGATAGTTCCCTTAATACTCCGGATAGAGTTTCTGAAGATATTGTAAGATGCATCTCTTCTATTTACTGCAAACTTGCCACTCCCCAAGCTCAAGTTGCACTATCAGCTTCTCCTACTTCATCCTTAGCTTCATCAAGTATATTTTCTTCAAAGAATCCTTGTGACAGTTGGAGTCCACATTGCAATGACGATGCTACAACAGACCATGAAGGGTTCAAAGATAATAATGGACCATACACTACAATGATAGAAGTGTTGAAGATATGCTTAGATGATGACAGCTTCAATTATGCTGCTCAAATGCTACAAAATTTCAGGTCATTGGTTCGAAGTCTTGAGAAGGTTGATGTTCTGAAGATGAGGCGCGAAGAGAAGCTTGTATTCTGGATTAATATTCACAATGCCTTGGTGATGCATGCATTCTTGGCATATGGAACACATAATCGTGTGAAAAGTACTTCAATTTTGAAGGCAGCCTACAACATAGGGGGACATTTCATAAATGCATATGTCATACAGAGCTCCATTTTAGGAATTCGAGCGCACCATGCAGCACCGTGGCTACAATCATTACTGCATTCAGGAAAGAAGTTCAAGACAGGGAGCTTGAGGCACATCTACGCTCTAGAATACCCTGAGCCACTAGTCCACTTTGCTCTATGTTCTGGGGCTTACTCTGACCCAGCGGTTCGAGCATACACTGCAAAGAGTATATTCCAGGATCTGAAACTTGCTAAAAGAGAATTTATTCAAGCCAGTGTCTACACAGATAATGATACAAAGGTTTACCTCCCAAAAATTCTGGAGTACTTCGCAAAGGACATGTCACTATGTATCCGTGGACTCTTGGAAGAAGTAGAGGAATGTCTCTCAGAAATTCAAAAGAAAGCTATCAGCAGCTGTAAGAAGGGGAGGCTTGAGGACAAGTCTGTACATTGGCAACCTCAAAGCTCAACATTTCGGTATGTGATCCATGAGGAATTAGCCACCGTAAGAATAGCAACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

426

Amino Acids

47.65

Weight (kDa)

8.15

Isoelectric Point (pI)

53.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF547 PF04784 209 - 340 1.5e-35 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1032
AccII CGCG 1 cut(s) 636
AccIII TCCGGA 1 cut(s) 289
AciI CCGC 2 cut(s) 213, 956
AclWI GGATC 3 cut(s) 255, 999, 1234
AcsI RAATTY 6 cut(s) 4, 580, 804, 1013, 1068, 1146
AcuI CTGAAG 3 cut(s) 162, 324, 644
AfaI GTAC 3 cut(s) 727, 1079, 1203
AflIII ACRYGT 1 cut(s) 1092
AhlI ACTAGT 1 cut(s) 913
AjnI CCWGG 2 cut(s) 114, 987
Alw21I GWGCWC 1 cut(s) 794
Alw26I GTCTC 2 cut(s) 60, 1142
AlwI GGATC 3 cut(s) 255, 999, 1234
AlwNI CAGNNNCTG 2 cut(s) 994, 1170
Aor13HI TCCGGA 1 cut(s) 289
ApeKI GCWGC 5 cut(s) 237, 563, 743, 821, 1167
ApoI RAATTY 6 cut(s) 4, 580, 804, 1013, 1068, 1146
ArsI GACNNNNNNTTYG 2 cut(s) 1079, 1111
AseI ATTAAT 1 cut(s) 660
Asp700I GAANNNNTTC 2 cut(s) 16, 1017
AspLEI GCGC 2 cut(s) 636, 814
AspS9I GGNCC 2 cut(s) 202, 500
AsuHPI GGTGA 1 cut(s) 691
AsuII TTCGAA 1 cut(s) 598
AvaII GGWCC 2 cut(s) 202, 500
BaeI ACNNNNGTAYC 2 cut(s) 1038, 1071
BanII GRGCYC 1 cut(s) 794
BarI GAAGNNNNNNTAC 4 cut(s) 400, 432, 731, 763
Bbv12I GWGCWC 1 cut(s) 794
BbvI GCAGC 5 cut(s) 249, 550, 755, 833, 1179
BciT130I CCWGG 2 cut(s) 116, 989
BciVI GTATCC 1 cut(s) 1115
BcoDI GTCTC 2 cut(s) 60, 1142
BcuI ACTAGT 1 cut(s) 913
BfaI CTAG 4 cut(s) 60, 254, 896, 914
BfuI GTATCC 1 cut(s) 1115
BisI GCNGC 5 cut(s) 238, 564, 744, 822, 1168
BlsI GCNGC 5 cut(s) 239, 565, 745, 823, 1169
BmcAI AGTACT 2 cut(s) 727, 1079
Bme1390I CCNGG 2 cut(s) 116, 989
Bme18I GGWCC 2 cut(s) 202, 500
BmgT120I GGNCC 2 cut(s) 202, 500
BmrFI CCNGG 2 cut(s) 116, 989
BmsI GCATC 4 cut(s) 308, 330, 451, 672
BpmI CTGGAG 1 cut(s) 1094
Bpu10I CCTNAGC 2 cut(s) 394, 906
Bpu14I TTCGAA 1 cut(s) 598
BpuEI CTTGAG 4 cut(s) 345, 626, 898, 1208
BsaJI CCNNGG 4 cut(s) 241, 675, 827, 1108
BsaWI WCCGGW 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 1026
Bse3DI GCAATG 2 cut(s) 448, 460
BseAI TCCGGA 1 cut(s) 289
BseBI CCWGG 2 cut(s) 116, 989
BseDI CCNNGG 4 cut(s) 241, 675, 827, 1108
BseGI GGATG 1 cut(s) 389
BseMI GCAATG 2 cut(s) 448, 460
BseMII CTCAG 2 cut(s) 897, 1155
BseNI ACTGG 1 cut(s) 1026
BseXI GCAGC 5 cut(s) 249, 550, 755, 833, 1179
BseYI CCCAGC 1 cut(s) 952
Bsh1236I CGCG 1 cut(s) 636
BsiHKAI GWGCWC 1 cut(s) 794
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 774
BsmAI GTCTC 2 cut(s) 60, 1142
BsmFI GGGAC 1 cut(s) 774
BsmI GAATGC 2 cut(s) 689, 847
Bsp119I TTCGAA 1 cut(s) 598
Bsp1286I GDGCHC 1 cut(s) 794
Bsp13I TCCGGA 1 cut(s) 289
Bsp1407I TGTACA 1 cut(s) 1201
Bsp143I GATC 3 cut(s) 247, 991, 1239
BspACI CCGC 2 cut(s) 213, 956
BspCNI CTCAG 2 cut(s) 898, 1154
BspEI TCCGGA 1 cut(s) 289
BspFNI CGCG 1 cut(s) 636
BspPI GGATC 3 cut(s) 255, 999, 1234
BspQI GCTCTTC 1 cut(s) 7
BspT104I TTCGAA 1 cut(s) 598
BsrDI GCAATG 2 cut(s) 448, 460
BsrGI TGTACA 1 cut(s) 1201
BsrI ACTGG 1 cut(s) 1026
BssECI CCNNGG 4 cut(s) 241, 675, 827, 1108
BssMI GATC 3 cut(s) 247, 991, 1239
BssT1I CCWWGG 2 cut(s) 241, 675
Bst2UI CCWGG 2 cut(s) 116, 989
Bst4CI ACNGT 5 cut(s) 93, 150, 437, 828, 1261
Bst6I CTCTTC 4 cut(s) 7, 175, 331, 633
BstAPI GCANNNNNTGC 1 cut(s) 971
BstAUI TGTACA 1 cut(s) 1201
BstBI TTCGAA 1 cut(s) 598
BstC8I GCNNGC 1 cut(s) 687
BstDEI CTNAG 4 cut(s) 394, 538, 906, 1141
BstDSI CCRYGG 2 cut(s) 827, 1108
BstF5I GGATG 1 cut(s) 389
BstFNI CGCG 1 cut(s) 636
BstHHI GCGC 2 cut(s) 636, 814
BstKTI GATC 3 cut(s) 250, 994, 1242
BstMAI GTCTC 2 cut(s) 60, 1142
BstMBI GATC 3 cut(s) 247, 991, 1239
BstMWI GCNNNNNNNGC 7 cut(s) 237, 365, 374, 695, 818, 962, 971
BstNI CCWGG 2 cut(s) 116, 989
BstNSI RCATGY 2 cut(s) 689, 1096
BstSCI CCNGG 2 cut(s) 114, 987
BstUI CGCG 1 cut(s) 636
BstV1I GCAGC 5 cut(s) 249, 550, 755, 833, 1179
BstX2I RGATCY 1 cut(s) 991
BstYI RGATCY 1 cut(s) 991
BsuI GTATCC 1 cut(s) 1115
BtgI CCRYGG 2 cut(s) 827, 1108
BtgZI GCGATG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 389
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 3 cut(s) 146, 969, 1033
Cac8I GCNNGC 1 cut(s) 687
CaiI CAGNNNCTG 2 cut(s) 994, 1170
CfoI GCGC 2 cut(s) 636, 814
Cfr13I GGNCC 2 cut(s) 202, 500
Csp6I GTAC 3 cut(s) 726, 1078, 1202
CviAII CATG 5 cut(s) 476, 686, 818, 1093, 1244
CviQI GTAC 3 cut(s) 726, 1078, 1202
DdeI CTNAG 4 cut(s) 394, 538, 906, 1141
DpnI GATC 3 cut(s) 249, 993, 1241
DpnII GATC 3 cut(s) 247, 991, 1239
Eam1104I CTCTTC 4 cut(s) 7, 175, 331, 633
EarI CTCTTC 4 cut(s) 7, 175, 331, 633
Ecl136II GAGCTC 1 cut(s) 792
Eco130I CCWWGG 2 cut(s) 241, 675
Eco24I GRGCYC 1 cut(s) 794
Eco47I GGWCC 2 cut(s) 202, 500
Eco53kI GAGCTC 1 cut(s) 792
Eco57I CTGAAG 3 cut(s) 162, 324, 644
EcoICRI GAGCTC 1 cut(s) 792
EcoRI GAATTC 2 cut(s) 4, 804
EcoRII CCWGG 2 cut(s) 114, 987
EcoT14I CCWWGG 2 cut(s) 241, 675
EcoT22I ATGCAT 4 cut(s) 323, 687, 691, 778
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 241, 675
FaeI CATG 5 cut(s) 479, 689, 821, 1096, 1247
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 630, 662
FaqI GGGAC 1 cut(s) 774
FatI CATG 5 cut(s) 475, 685, 817, 1092, 1243
FauNDI CATATG 2 cut(s) 700, 778
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 5 cut(s) 238, 564, 744, 822, 1168
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 1 cut(s) 794
Fsp4HI GCNGC 5 cut(s) 238, 564, 744, 822, 1168
FspBI CTAG 4 cut(s) 60, 254, 896, 914
GlaI GCGC 2 cut(s) 635, 813
GluI GCNGC 5 cut(s) 238, 564, 744, 822, 1168
GsaI CCCAGC 1 cut(s) 956
GsuI CTGGAG 1 cut(s) 1094
HapII CCGG 1 cut(s) 290
HhaI GCGC 2 cut(s) 636, 814
Hin1II CATG 5 cut(s) 479, 689, 821, 1096, 1247
Hin6I GCGC 2 cut(s) 634, 812
HinP1I GCGC 2 cut(s) 634, 812
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 5 cut(s) 134, 218, 424, 442, 1113
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 5 cut(s) 446, 919, 1033, 1057, 1112
Hpy188I TCNGA 8 cut(s) 22, 139, 181, 304, 624, 949, 996, 1144
Hpy188III TCNNGA 8 cut(s) 9, 290, 605, 655, 852, 865, 896, 1073
Hpy8I GTNNAC 5 cut(s) 446, 919, 1033, 1057, 1112
HpyAV CCTTC 5 cut(s) 136, 473, 604, 733, 1171
HpyCH4III ACNGT 5 cut(s) 93, 150, 437, 828, 1261
HpyF10VI GCNNNNNNNGC 7 cut(s) 237, 365, 374, 695, 818, 962, 971
HpyF3I CTNAG 4 cut(s) 394, 538, 906, 1141
Hsp92II CATG 5 cut(s) 479, 689, 821, 1096, 1247
HspAI GCGC 2 cut(s) 634, 812
Kpn2I TCCGGA 1 cut(s) 289
Kzo9I GATC 3 cut(s) 247, 991, 1239
LguI GCTCTTC 1 cut(s) 7
LmnI GCTCC 2 cut(s) 797, 873
Lsp1109I GCAGC 5 cut(s) 249, 550, 755, 833, 1179
LweI GCATC 4 cut(s) 308, 330, 451, 672
MaeI CTAG 4 cut(s) 60, 254, 896, 914
MaeIII GTNAC 3 cut(s) 131, 431, 1095
MalI GATC 3 cut(s) 249, 993, 1241
MboI GATC 3 cut(s) 247, 991, 1239
MflI RGATCY 1 cut(s) 991
MhlI GDGCHC 1 cut(s) 794
MluCI AATT 9 cut(s) 4, 556, 580, 732, 804, 1013, 1068, 1146, 1250
MlyI GAGTC 3 cut(s) 128, 451, 1107
MmeI TCCRAC 3 cut(s) 75, 215, 419
MnlI CCTC 8 cut(s) 624, 873, 1070, 1123, 1176, 1183, 1224, 1240
Mph1103I ATGCAT 4 cut(s) 323, 687, 691, 778
MroI TCCGGA 1 cut(s) 289
MroXI GAANNNNTTC 2 cut(s) 16, 1017
MseI TTAA 3 cut(s) 78, 282, 660
MslI CAYNNNNRTG 4 cut(s) 255, 453, 512, 714
MspA1I CMGCKG 3 cut(s) 215, 956, 1170
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 116, 989
Mva1269I GAATGC 2 cut(s) 689, 847
MvaI CCWGG 2 cut(s) 116, 989
MvnI CGCG 1 cut(s) 636
MwoI GCNNNNNNNGC 7 cut(s) 237, 365, 374, 695, 818, 962, 971
NdeI CATATG 2 cut(s) 700, 778
NdeII GATC 3 cut(s) 247, 991, 1239
NlaIII CATG 5 cut(s) 479, 689, 821, 1096, 1247
NmuCI GTSAC 3 cut(s) 131, 431, 1095
NsiI ATGCAT 4 cut(s) 323, 687, 691, 778
NspI RCATGY 2 cut(s) 689, 1096
NspV TTCGAA 1 cut(s) 598
PaeI GCATGC 1 cut(s) 689
PciI ACATGT 1 cut(s) 1092
PciSI GCTCTTC 1 cut(s) 7
PctI GAATGC 2 cut(s) 689, 847
PdmI GAANNNNTTC 2 cut(s) 16, 1017
PfeI GAWTC 2 cut(s) 218, 424
PfoI TCCNGGA 1 cut(s) 987
PkrI GCNGC 5 cut(s) 239, 565, 745, 823, 1169
PleI GAGTC 3 cut(s) 128, 450, 1107
PpsI GAGTC 3 cut(s) 128, 450, 1107
PscI ACATGT 1 cut(s) 1092
PshBI ATTAAT 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 794
Psp6I CCWGG 2 cut(s) 114, 987
PspFI CCCAGC 1 cut(s) 952
PspGI CCWGG 2 cut(s) 114, 987
PspPI GGNCC 2 cut(s) 202, 500
PstNI CAGNNNCTG 2 cut(s) 994, 1170
PsuI RGATCY 1 cut(s) 991
PvuII CAGCTG 1 cut(s) 1170
RsaI GTAC 3 cut(s) 727, 1079, 1203
RsaNI GTAC 3 cut(s) 726, 1078, 1202
RseI CAYNNNNRTG 4 cut(s) 255, 453, 512, 714
SacI GAGCTC 1 cut(s) 794
SapI GCTCTTC 1 cut(s) 7
SaqAI TTAA 3 cut(s) 78, 282, 660
SatI GCNGC 5 cut(s) 238, 564, 744, 822, 1168
Sau3AI GATC 3 cut(s) 247, 991, 1239
Sau96I GGNCC 2 cut(s) 202, 500
ScaI AGTACT 2 cut(s) 727, 1079
SchI GAGTC 3 cut(s) 128, 451, 1107
ScrFI CCNGG 2 cut(s) 116, 989
SduI GDGCHC 1 cut(s) 794
SfaNI GCATC 4 cut(s) 308, 330, 451, 672
SfuI TTCGAA 1 cut(s) 598
SinI GGWCC 2 cut(s) 202, 500
SmiMI CAYNNNNRTG 4 cut(s) 255, 453, 512, 714
SmlI CTYRAG 4 cut(s) 360, 605, 877, 1187
SmoI CTYRAG 4 cut(s) 360, 605, 877, 1187
SpeI ACTAGT 1 cut(s) 913
SphI GCATGC 1 cut(s) 689
Sse9I AATT 9 cut(s) 4, 556, 580, 732, 804, 1013, 1068, 1146, 1250
SsiI CCGC 2 cut(s) 213, 956
SspI AATATT 1 cut(s) 664
SspMI CTAG 4 cut(s) 60, 254, 896, 914
SstI GAGCTC 1 cut(s) 794
StyD4I CCNGG 2 cut(s) 114, 987
StyI CCWWGG 2 cut(s) 241, 675
TaaI ACNGT 5 cut(s) 93, 150, 437, 828, 1261
TaqI TCGA 3 cut(s) 598, 808, 961
TasI AATT 9 cut(s) 4, 556, 580, 732, 804, 1013, 1068, 1146, 1250
TatI WGTACW 3 cut(s) 725, 1077, 1201
TfiI GAWTC 2 cut(s) 218, 424
Tru1I TTAA 3 cut(s) 78, 282, 660
Tru9I TTAA 3 cut(s) 78, 282, 660
TscAI CASTG 3 cut(s) 153, 976, 1033
TseFI GTSAC 3 cut(s) 131, 431, 1095
TseI GCWGC 5 cut(s) 237, 563, 743, 821, 1167
Tsp45I GTSAC 3 cut(s) 131, 431, 1095
TspDTI ATGAA 5 cut(s) 17, 378, 390, 492, 757
TspGWI ACGGA 1 cut(s) 1097
TspRI CASTG 3 cut(s) 153, 976, 1033
VpaK11BI GGWCC 2 cut(s) 202, 500
VspI ATTAAT 1 cut(s) 660
XapI RAATTY 6 cut(s) 4, 580, 804, 1013, 1068, 1146
XbaI TCTAGA 1 cut(s) 895
XceI RCATGY 2 cut(s) 689, 1096
XmiI GTMKAC 1 cut(s) 1032
XmnI GAANNNNTTC 2 cut(s) 16, 1017
XspI CTAG 4 cut(s) 60, 254, 896, 914
ZrmI AGTACT 2 cut(s) 727, 1079
Zsp2I ATGCAT 4 cut(s) 323, 687, 691, 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.