AT1G76620

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005669
Physical Location & Seq
Forward (+)
28756521 .. 28759332
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76620.1

Sequence Viewer

Length: 1584 bp
ATGATGGCTCTGATGAATTGTGCATATTCATCTTCTTCTTCGTCTTCGTCTACTTCCTCTCCCACTTCTTCATCATCTTCTTCTTCATATTCTCCAAGGAACAGAGAGAGCATGTTTTTGCAGAGTGTAGTTGATGCGTCACCTTCAAGTTTCTCTTCCGACAGAAAATCTATTTTACCAATTGAGAAGACTTTAATAAGACATCAGAATTCCAGTCTCTGCTTGATCCCAAAGTCTTCAGAAGAATTGAAGAAGGAGATTGCATCAATTGAGATAGAGATTCTACACATGGAACGATATCTCTTATCTCTTTACAGAAAATCATTTGAGCAACAACTTCCTAATTCCTTCTCTAATCTCTCTGTTACTACTACCTTACCTCGTTCTGTGACTACTTCACCAACATCACTAACTCATTATCAAGCTTACCAAAAACCAATCTCATATCCTCGGAGTTTCAACACTAGTTTAAAAGCCTTATCTTCAAGGGAAGGGACTAGAGTAGTTTCTGGTACTCACAGTCTTGGAGAGCTTTTAGGATCATCTCATATAGTTGATCATAACAACTTCATAAACCCGAATAAACTCTCTGAAGATATCATGAGATGCATTTCTTCTGTGTATTGTACACTTTCAAGAGGATCAACGAGTACCACTTCCACTTGTTTTCCTGCTTCTCCGGTTTCCTCCAATGCCTCAACCATTTTCTCTTCCAAATTTAATTATGAAGATAAATGGAGCTTGAATGGCGCAAGTGAGGATCATTTTCTGAATCACTGTCAAGATCAAGACAATGTTTTGCCTTGTGGTGTTGTTGTCATAGAGGCTCTTAGAGTACATTTAGACGATGGTAGTTTCGGTTACGCTGCTCTTATGCTTCAAAACTTCAGATCACTGGTTCAAAATCTTGAGAAAGTTGATCCGAGTAGAATGAAACGCGAAGAGAAGCTCGCGTTTTGGATAAACATTCACAATGCTCTTGTGATGCATGCTTACTTAGCTTATGGAACTCACAACCGCGCAAGAAACACATCGGTTTTGAAGGCAGCTTATGATATTGGAGGCTATAGAATAAACCCTTATATCATCCAAAGCTCAATCTTAGGAATCAGACCTCATTACACATCACCATCACCTTTACTCCAAACTCTGTTTTCCCCTTCAAGAAAGTCGAAAACTTGCAGTGTTAGACACATTTACGCATTGGAATACCCCGAGGCTTTAGCTCATTTCGCTATTTCTTCAGGGGCATTCACTGATCCTACAGTTCGAGTTTACACCGCGGATAGAATCTTCCGGGATCTAAGACAAGCGAAACAAGAGTATATCAGAAGTAATGTTCGTGTTTACAAAGGGACAAAGATTTTGTTGCCAAAGATCTTTCAACATTATGTTAAAGACATGTCAATGGATGTGTCTAAGCTCATGGAAGCAACATCTCAATGTTTACCAGAAGATGCAAGGAAAATTGCTGAGAAATGTTTGAAAGAGAAGAAGAGTAAGAATTTTGAATGGTTACCGGAAAATTTGAGCTTCCGGTATGTTATCGCCGGAGAATTGGTCGGAGCAAGAAATAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

527

Amino Acids

59.17

Weight (kDa)

9.23

Isoelectric Point (pI)

58.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 69 - 111 4.9e-07 Leucine-zipper of ternary complex factor MIP1
DUF547 PF04784 309 - 443 5.9e-33 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 50
AccII CGCG 4 cut(s) 939, 953, 1020, 1283
AciI CCGC 3 cut(s) 1018, 1281, 1283
AclWI GGATC 7 cut(s) 220, 547, 649, 768, 914, 1253, 1308
AcsI RAATTY 4 cut(s) 208, 716, 1504, 1525
AcuI CTGAAG 4 cut(s) 222, 612, 871, 1227
AfaI GTAC 4 cut(s) 514, 628, 652, 837
AflIII ACRYGT 1 cut(s) 1401
AhlI ACTAGT 1 cut(s) 464
AjiI CACGTC 1 cut(s) 1581
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 7 cut(s) 220, 547, 649, 768, 914, 1253, 1308
AlwNI CAGNNNCTG 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 1214
ApeKI GCWGC 2 cut(s) 866, 1046
ApoI RAATTY 4 cut(s) 208, 716, 1504, 1525
ArsI GACNNNNNNTTYG 2 cut(s) 1348, 1380
AspLEI GCGC 2 cut(s) 752, 1022
AsuC2I CCSGG 1 cut(s) 1298
AsuHPI GGTGA 4 cut(s) 132, 390, 1119, 1125
AvaI CYCGRG 1 cut(s) 1214
BbsI GAAGAC 3 cut(s) 36, 194, 228
BbvI GCAGC 2 cut(s) 853, 1058
BccI CCATC 2 cut(s) 842, 1138
BclI TGATCA 1 cut(s) 556
BcnI CCSGG 1 cut(s) 1298
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 2 cut(s) 465, 498
BfmI CTRYAG 2 cut(s) 1066, 1263
BglII AGATCT 1 cut(s) 1377
BisI GCNGC 2 cut(s) 867, 1047
BlsI GCNGC 2 cut(s) 868, 1048
Bme1390I CCNGG 1 cut(s) 1298
BmeT110I CYCGRG 1 cut(s) 1214
BmgBI CACGTC 1 cut(s) 1581
BmrFI CCNGG 1 cut(s) 1298
BmsI GCATC 5 cut(s) 124, 272, 596, 975, 1447
BpiI GAAGAC 3 cut(s) 36, 194, 228
BpuEI CTTGAG 1 cut(s) 929
BpuMI CCSGG 1 cut(s) 1298
BsaJI CCNNGG 4 cut(s) 95, 449, 1215, 1281
BsaWI WCCGGW 3 cut(s) 679, 1519, 1536
BsaXI ACNNNNNCTCC 6 cut(s) 43, 73, 1125, 1155, 1545, 1575
Bse1I ACTGG 2 cut(s) 213, 900
BseDI CCNNGG 4 cut(s) 95, 449, 1215, 1281
BseGI GGATG 2 cut(s) 1086, 1417
BseMII CTCAG 1 cut(s) 1464
BseNI ACTGG 2 cut(s) 213, 900
BseXI GCAGC 2 cut(s) 853, 1058
Bsh1236I CGCG 4 cut(s) 939, 953, 1020, 1283
BsiHKCI CYCGRG 1 cut(s) 1214
BsiSI CCGG 5 cut(s) 680, 1297, 1520, 1537, 1551
BslFI GGGAC 2 cut(s) 508, 1369
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 2 cut(s) 508, 1369
BsmI GAATGC 1 cut(s) 1250
BsoBI CYCGRG 1 cut(s) 1214
Bsp1407I TGTACA 1 cut(s) 626
BspACI CCGC 3 cut(s) 1018, 1281, 1283
BspCNI CTCAG 1 cut(s) 1465
BspFNI CGCG 4 cut(s) 939, 953, 1020, 1283
BspHI TCATGA 1 cut(s) 600
BspPI GGATC 7 cut(s) 220, 547, 649, 768, 914, 1253, 1308
BsrGI TGTACA 1 cut(s) 626
BsrI ACTGG 2 cut(s) 213, 900
BssECI CCNNGG 4 cut(s) 95, 449, 1215, 1281
BssT1I CCWWGG 1 cut(s) 95
Bst4CI ACNGT 3 cut(s) 521, 779, 1267
Bst6I CTCTTC 4 cut(s) 160, 715, 936, 1490
BstAUI TGTACA 1 cut(s) 626
BstC8I GCNNGC 2 cut(s) 951, 990
BstDEI CTNAG 6 cut(s) 830, 997, 1102, 1304, 1419, 1473
BstDSI CCRYGG 1 cut(s) 1281
BstEII GGTNACC 1 cut(s) 1515
BstF5I GGATG 2 cut(s) 1086, 1417
BstFNI CGCG 4 cut(s) 939, 953, 1020, 1283
BstHHI GCGC 2 cut(s) 752, 1022
BstMAI GTCTC 1 cut(s) 221
BstMWI GCNNNNNNNGC 3 cut(s) 747, 998, 1232
BstNSI RCATGY 3 cut(s) 115, 992, 1405
BstPI GGTNACC 1 cut(s) 1515
BstSCI CCNGG 1 cut(s) 1296
BstSFI CTRYAG 2 cut(s) 1066, 1263
BstUI CGCG 4 cut(s) 939, 953, 1020, 1283
BstV1I GCAGC 2 cut(s) 853, 1058
BstV2I GAAGAC 3 cut(s) 36, 194, 228
BstX2I RGATCY 2 cut(s) 1300, 1377
BstYI RGATCY 2 cut(s) 1300, 1377
BtgI CCRYGG 1 cut(s) 1281
BtrI CACGTC 1 cut(s) 1581
BtsCI GGATG 2 cut(s) 1086, 1417
BtsI GCAGTG 1 cut(s) 1189
BtsIMutI CAGTG 4 cut(s) 775, 893, 1189, 1254
Cac8I GCNNGC 2 cut(s) 951, 990
CaiI CAGNNNCTG 1 cut(s) 219
CciI TCATGA 1 cut(s) 600
CfoI GCGC 2 cut(s) 752, 1022
Cfr42I CCGCGG 1 cut(s) 1284
CseI GACGC 1 cut(s) 126
Csp6I GTAC 4 cut(s) 513, 627, 651, 836
CviAII CATG 6 cut(s) 112, 289, 601, 989, 1402, 1426
CviQI GTAC 4 cut(s) 513, 627, 651, 836
DdeI CTNAG 6 cut(s) 830, 997, 1102, 1304, 1419, 1473
DraI TTTAAA 1 cut(s) 471
Eam1104I CTCTTC 4 cut(s) 160, 715, 936, 1490
EarI CTCTTC 4 cut(s) 160, 715, 936, 1490
Eco130I CCWWGG 1 cut(s) 95
Eco32I GATATC 2 cut(s) 299, 598
Eco57I CTGAAG 4 cut(s) 222, 612, 871, 1227
Eco88I CYCGRG 1 cut(s) 1214
Eco91I GGTNACC 1 cut(s) 1515
EcoO65I GGTNACC 1 cut(s) 1515
EcoRI GAATTC 1 cut(s) 208
EcoRV GATATC 2 cut(s) 299, 598
EcoT14I CCWWGG 1 cut(s) 95
EcoT22I ATGCAT 2 cut(s) 611, 990
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 6 cut(s) 115, 292, 604, 992, 1405, 1429
FalI AAGNNNNNCTT 2 cut(s) 139, 171
FaqI GGGAC 2 cut(s) 508, 1369
FatI CATG 6 cut(s) 111, 288, 600, 988, 1401, 1425
FbaI TGATCA 1 cut(s) 556
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 2 cut(s) 867, 1047
FokI GGATG 2 cut(s) 1073, 1424
Fsp4HI GCNGC 2 cut(s) 867, 1047
FspBI CTAG 2 cut(s) 465, 498
GlaI GCGC 2 cut(s) 751, 1021
GluI GCNGC 2 cut(s) 867, 1047
HapII CCGG 5 cut(s) 680, 1297, 1520, 1537, 1551
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 752, 1022
Hin1II CATG 6 cut(s) 115, 292, 604, 992, 1405, 1429
Hin6I GCGC 2 cut(s) 750, 1020
HinP1I GCGC 2 cut(s) 750, 1020
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 4 cut(s) 280, 772, 1107, 1290
HpaII CCGG 5 cut(s) 680, 1297, 1520, 1537, 1551
HphI GGTGA 4 cut(s) 132, 390, 1119, 1125
Hpy166II GTNNAC 5 cut(s) 51, 629, 1276, 1348, 1448
Hpy188III TCNNGA 6 cut(s) 601, 636, 782, 788, 908, 1164
Hpy8I GTNNAC 5 cut(s) 51, 629, 1276, 1348, 1448
HpyAV CCTTC 6 cut(s) 153, 247, 358, 485, 1036, 1170
HpyCH4III ACNGT 3 cut(s) 521, 779, 1267
HpyCH4IV ACGT 1 cut(s) 1580
HpyCH4V TGCA 7 cut(s) 23, 121, 263, 609, 988, 1182, 1460
HpyF10VI GCNNNNNNNGC 3 cut(s) 747, 998, 1232
HpyF3I CTNAG 6 cut(s) 830, 997, 1102, 1304, 1419, 1473
HpySE526I ACGT 1 cut(s) 1580
Hsp92II CATG 6 cut(s) 115, 292, 604, 992, 1405, 1429
HspAI GCGC 2 cut(s) 750, 1020
Ksp22I TGATCA 1 cut(s) 556
KspI CCGCGG 1 cut(s) 1284
LmnI GCTCC 2 cut(s) 738, 1565
Lsp1109I GCAGC 2 cut(s) 853, 1058
LweI GCATC 5 cut(s) 124, 272, 596, 975, 1447
MaeI CTAG 2 cut(s) 465, 498
MaeII ACGT 1 cut(s) 1580
MaeIII GTNAC 5 cut(s) 138, 364, 388, 860, 1515
MfeI CAATTG 2 cut(s) 180, 267
MflI RGATCY 2 cut(s) 1300, 1377
MmeI TCCRAC 2 cut(s) 183, 1543
Mph1103I ATGCAT 2 cut(s) 611, 990
MseI TTAA 4 cut(s) 194, 470, 720, 1395
MslI CAYNNNNRTG 2 cut(s) 1406, 1441
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 5 cut(s) 680, 1297, 1520, 1537, 1551
MspR9I CCNGG 1 cut(s) 1298
MunI CAATTG 2 cut(s) 180, 267
Mva1269I GAATGC 1 cut(s) 1250
MvnI CGCG 4 cut(s) 939, 953, 1020, 1283
MwoI GCNNNNNNNGC 3 cut(s) 747, 998, 1232
NciI CCSGG 1 cut(s) 1298
NlaIII CATG 6 cut(s) 115, 292, 604, 992, 1405, 1429
NmuCI GTSAC 2 cut(s) 138, 388
NsiI ATGCAT 2 cut(s) 611, 990
NspI RCATGY 3 cut(s) 115, 992, 1405
PaeI GCATGC 1 cut(s) 992
PagI TCATGA 1 cut(s) 600
PciI ACATGT 1 cut(s) 1401
PctI GAATGC 1 cut(s) 1250
PfeI GAWTC 4 cut(s) 280, 772, 1107, 1290
PfoI TCCNGGA 1 cut(s) 1296
PkrI GCNGC 2 cut(s) 868, 1048
PscI ACATGT 1 cut(s) 1401
PspEI GGTNACC 1 cut(s) 1515
PstNI CAGNNNCTG 1 cut(s) 219
PsuI RGATCY 2 cut(s) 1300, 1377
RsaI GTAC 4 cut(s) 514, 628, 652, 837
RsaNI GTAC 4 cut(s) 513, 627, 651, 836
RseI CAYNNNNRTG 2 cut(s) 1406, 1441
SacII CCGCGG 1 cut(s) 1284
SaqAI TTAA 4 cut(s) 194, 470, 720, 1395
SatI GCNGC 2 cut(s) 867, 1047
ScrFI CCNGG 1 cut(s) 1298
SfaNI GCATC 5 cut(s) 124, 272, 596, 975, 1447
SfcI CTRYAG 2 cut(s) 1066, 1263
Sfr303I CCGCGG 1 cut(s) 1284
SgrBI CCGCGG 1 cut(s) 1284
SmiMI CAYNNNNRTG 2 cut(s) 1406, 1441
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
SpeI ACTAGT 1 cut(s) 464
SphI GCATGC 1 cut(s) 992
SsiI CCGC 3 cut(s) 1018, 1281, 1283
SspMI CTAG 2 cut(s) 465, 498
StyD4I CCNGG 1 cut(s) 1296
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 521, 779, 1267
TaiI ACGT 1 cut(s) 1583
TaqI TCGA 2 cut(s) 1172, 1270
TatI WGTACW 2 cut(s) 626, 835
TfiI GAWTC 4 cut(s) 280, 772, 1107, 1290
Tru1I TTAA 4 cut(s) 194, 470, 720, 1395
Tru9I TTAA 4 cut(s) 194, 470, 720, 1395
TscAI CASTG 4 cut(s) 782, 900, 1189, 1261
TseFI GTSAC 2 cut(s) 138, 388
TseI GCWGC 2 cut(s) 866, 1046
Tsp45I GTSAC 2 cut(s) 138, 388
TspDTI ATGAA 7 cut(s) 18, 29, 60, 75, 559, 741, 947
TspRI CASTG 4 cut(s) 782, 900, 1189, 1261
XapI RAATTY 4 cut(s) 208, 716, 1504, 1525
XceI RCATGY 3 cut(s) 115, 992, 1405
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 2 cut(s) 465, 498
Zsp2I ATGCAT 2 cut(s) 611, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.